This experiment is likely to have possible errors if do not do it carefully.

Mistakes made here

can ruin the sample and compromise any observations wished to make. One of the most
issues we see is poor slide preparation The experiment requires that all necessary
requirements be completely prepared. It is also necessary to ensure that the equipment is
clean before use. By letting the slide have some air, it will obscure what's in the biomass. As
well as trying to see the object around smudges and fingerprints on the slide. Next is
insufficient magnification. Simply put, if not enlarge the microscope image enough, will not
be able to see what is present. Or may miss smaller filaments. The same is true for being
unable to stay focussed. If the image is blurry, it is most likely out of focus, like this one. In
addition, students frequently make the mistake of using too much light. Quite often, the
picture is simply not in focus. Don't turn the focus knob too far in one direction, or a clear
view might not be available to see. Some people turn the focus knob back and forth and
have a problem finding a clear picture. This is a particular problem for the higher
magnifications, where the focus can be lost much more easily.Another possible error that
could have occurred in the collection of the data is the wrong focus plane. It might be able to
see a sharp image, but not of the object that needs looking at. In this case, don't focus on
the specimen itself but on the dust on the surface of the slide or cover glass. It may also be
the case that the dust is focused on the lens of the condenser, which is beneath the slide.
Simply move the slide back and forth. If the dust stays stationary and does not move, then
do not focus on the slide. Slide not center is another common problem. Maybe nothing can
be seen because the eyes were looking at a place on the slide that does not contain a
specimen at all. If a large and dark specimen is placed on the stage, then the light of the
microscope is not able to pass through the object. Nothing can be seen except a dark
shadow without much detail. A dirty objective can greatly reduce the image quality. This can
be the case, when non-oil immersion objective becomes covered with immersion oil and if it
is not cleaned. Dust and dirt will stick to the oil and block the light.

Therefore, we need to improve the experiment if it would be repeated. First, about the slide
preparation. Handle the slide with care. Hold them by their sides and make a quick visual
check before putting them under the microscope. Always check if there is something that
looks odd or something that shouldn't be there. Begin with the lowest magnification for
sufficient magnification. Maintain the focus on the image. Then move to the highest
magnification for image capture. To stay focused on the image, try to focus most on the item
of concern. Most microscope have two focus knobs. The first focus knob is for coarse focus
and the second is for fine focus. Avoid using too much light. It is important that can reduce
the light so that the details of the subject can be seen clearly. If the image is not focused,
then it is much faster to simply start over again. Rotate the low-magnification objective into
place again and re-focus and re-center the image before moving the higher magnifications. If
the wrong focus plane is selected, it is best to start over and refocus with the low
magnification objective in place. Simply move the slide back and forth. Do not focus on the
slide if the dust remains still and does not move. Always make sure the slide is centred
before starting the observation. Look at the slide on the stage with the lamp turned on. The
specimen should be able to see in the light right above the condenser.
In this experiment, there are differences between prokaryote and eukaryote cells. A
prokaryote cell differs from an eukaryote cell in that one is unicellular while the other is
mostly multicellular. For the eukaryote cells, chicken liver and onion root tip cells were used
in the experiment. The experiment revealed that the liver cells of the chicken have a plasma
membrane and a visible nucleus. Meanwhile, in the onion root tip cell, the same cell as the
chicken's liver cell is found, which has a plasma membrane and nucleus. Additionally to the
cytoplasm and cell wall, plant cells have both. The cell wall is the outer layer that holds the
cell together and protects it from mechanical damage. It’s very strong because it’s made
from cellulose and protein. The second layer of the cell is plasma membrane. Cytoplasm is
the gel-like fluid inside the cell. It is used as a medium for chemical reactions. For prokaryote
cells, plain yoghurt is used as the experimental material. According to the observations,
there is a lack of a cell nucleus or any membrane-encased organelles.