Marine Oil FAMEs on FAMEWAX

Peaks
1. C14:0
2. C14:1
3. C16:0
4. C16:1
5. C18:0
3 6. C18:1 (oleate)
7. C18:1 (vaccenate)
8. C18:2 n6 c
9. C18:3 n3
10. C20:0
11. C20:1 n9
12. C20:2 n6
13. C20:4 n6
14. C20:3 n3
15. C20:5 n3
16. C22:0
1 17. C22:1 n9
18. C24:0
6 19. C22:6 n3
20. C24:1

4 5
11 15
19
7
2 8 13
9 17
10 12 14 16 18 20

0 1 2 3 4 5 6 7 8 9 10
Time (min)

GC_FF00566

Column FAMEWAX, 30 m, 0.32 mm ID, 0.25 µm (cat.# 12498)

Sample Marine oil FAME mix (cat.# 35066)
Conc.: 10 mg/mL total
Injection
Inj. Vol.: 0.5 µL split (split ratio 150:1)
Inj. Temp.: 250 °C
Oven
Oven Temp.: 195 °C to 240 °C at 5 °C/min (hold 1 min)
Carrier Gas H2, constant flow
Linear Velocity: 62 cm/sec
Detector FID @ 250 °C

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