NAME: …………………………………………………………………………………….………….

INDEX NUMBER: …………………………………… SIGNATURE: ………………………….

530/3
BIOLOGY PAPER 3
(PRACTICAL)
Jul/Aug. 2023
Uganda Advanced Certificate of Education
BIOLOGY PAPER THREE
(PRACTICAL)
3 HOURS 15 MINUTES
Instructions
 Attempt all questions.
 Answers to the questions must be written in the spaces of the question
paper provided.
 Use sharp HB pencil for drawings.

FOR EXAMINERS’ USE ONLY

QUESTION Marks Examiner’s Signature & No.

Question No. 1

No. 2

No. 3

TOTAL

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1. You are provided with specimen K which is a freshly killed animal.
(a) Examine the external features of the specimen and classify with 2 reasons
each, the phylum and class of the specimen
(i) Features of the phylum
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Reasons (02 marks)
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(ii) Features of the class
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Reasons (02 marks)
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(b) From your observations of the external features. State with reasons, the sex of
specimen.
Sex of the specimen…………………………………………………………… (01 mark)
Reasons (02 marks)
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(c) (i) Examine the antennae, and describe how it is adapted to its function (03
marks)
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(ii) Measure the length of the antenna the rest of the body. Record your results
in mm (02 marks)

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(iii) Explain the significance of the ratio of the measurement in (c) (ii) in
promoting the survival of the specimen in its habitat (02 marks)

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(iv) Using a razor blade, cut off the antenna from its base. Draw the first 5
segment as observed from the microscope at medium power. Do not label
(03 marks)

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(d) Place the specimen dorsal side uppermost, and cut along the right lateral side
of the specimen, displace the alimentary canal to the left to expose the
structures on the ventral cuticle and structures involved with removal of waste
products. Draw and label (18 marks)

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 2. You are provided with specimen P and solution A and distilled water.
You are required to find out the effect of solution A on the specimen P.
Label petri dishes 1,2,3,4,5. Measure the solutions A and distilled water
according to the table below.

Volume of Volume of Number of Percentage volume Percentage

solution A distilled plasmolyzed of concentration number of
(cm3) water (cm3) cells of solution A (%) plasmolyzed cells
(%)
10 0

8 2

5 5

2 8

0 10

Peel off strips of the lower epidermis of the leaf of specimen P and put them
in each of the solutions in the petri dishes 1to 5. And leave it to stand for 15
minutes. After 15 minutes. Mount each epidermal strip from each solution
and view under medium power of a microscope. Count 20 purple colored
cells, out of which, count the number of plasmolyzed cells.

(a) Compute the percentage concentration of solution A and percentage

number of plasmolyzed cells and complete the table (07 ½ marks)

(b) Plot a graph to show the relationship between percentage concentration of

solution A and percentage plasmolysis (07 ½ marks)

(ON THE GRAPH PROVIDED)

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 (c) From the graph, state the percentage concentration of solution A that
would show 50% plasmolysis on the epidermal strip of specimen P (01
mark)

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(d) From the graph, explain the effect of the concentration of solution A on
the cells of epidermal strip of specimen P (05 marks)

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(e) Describe how important the physiological process investigated in the
experiment is to the plant (03 marks)

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 3. You are provided with specimen Q which is a plant organ.
(a) Cut it longitudinally and examine it. Describe the internal structure. (03
marks)

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(b) Remove one of the leaves of specimen Q. strip off a piece of the inner most
layer and place it on a slide with a drop of water ad cover it with a cover
slip. Examine under the medium power of the microscope.
(i) Describe the structure of a cell clearly seen (02 marks)

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(ii) Now examine under lower power of microscope. Count and record the
number of cells in the field of view (02 marks)
From the left to right ……………………………………………………………….

From top to the bottom ……………………………………………………………….

(c) (i) Remove the slide from the stage, measure the field of view using a
transparent ruler and record your results.
Field of view ………………………………………………………… mm (01 mark)
(ii). Convert the diameter of the field into micrometer (µm). show your working (1
marks)

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(iii) Calculate the actual length of the one cell in micrometer (µ). Show your
working (03 marks)

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(d) Return the slide having the tissue on the stage. Draw one cell from the
tissue being observed under medium power of a micrometer (03 marks)

(e) (i) Measure the length of your drawing in mm and record it (01 mark)

Length of the drawing ……………………………………………………………. mm

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 (ii). Calculate the magnification of your drawing. Show your working (03
marks)

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(iii). Amount a little piece of specimen Q and view under the medium power
of a microscope. Draw and label three (3) adjacent cells (06 marks)

(f) Explain how the structure of each of the cells from the specimen relates
to their function (04 marks)
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END

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