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  • Biological Design 101

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    Dimitri Sotomayor
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    A local farmer has hired a biological engineer to design a biosensor bacterium that can detect a toxic molecule that may be present in the farmer's water supply. The biosensor bacteria would express a red fluorescent protein if the toxic molecule is detected, allowing the farmer to regularly monitor its presence in his soil and water by adding samples to the biosensor strain each week. The engineer must choose biological parts like promoters, ribosome binding sites, and the fluorescent reporter protein to construct a strain that will function as the requested biosensor device.

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    Biological design 101

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    A local farmer has hired a biological engineer to design a biosensor bacterium that can detect a toxic molecule that may be present in the farmer's water supply. The biosensor bacteria would express a red fluorescent protein if the toxic molecule is detected, allowing the farmer to regularly monitor its presence in his soil and water by adding samples to the biosensor strain each week. The engineer must choose biological parts like promoters, ribosome binding sites, and the fluorescent reporter protein to construct a strain that will function as the requested biosensor device.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    2 views6 pages

    Biological Design 101

    Uploaded by

    Dimitri Sotomayor
    A local farmer has hired a biological engineer to design a biosensor bacterium that can detect a toxic molecule that may be present in the farmer's water supply. The biosensor bacteria would express a red fluorescent protein if the toxic molecule is detected, allowing the farmer to regularly monitor its presence in his soil and water by adding samples to the biosensor strain each week. The engineer must choose biological parts like promoters, ribosome binding sites, and the fluorescent reporter protein to construct a strain that will function as the requested biosensor device.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 6

    Biological design 101

    Based on your growing reputation as a creative and smart biological


    engineer, a local farmer of Urcuquí has hired you to design a
    biosensor bacterium, capable of activating the expression of a red
    fluorescent protein in response to the presence of a toxic molecule,
    potentially present in the water he uses to water his crops. It is
    important to mention that he wants to add a sample of the soil and the
    water to your biosensor bacteria weekly, to constantly monitor the
    presence of the toxic molecule in his lands.
    1.- From the list below, choose the parts you
    will need to construct the biosensor strain

    SCP RBS T SIP TFBS TFBS TFBS

    RFP RBS LCP RBS T

    TATF T T LIP
    RBS T

    T LIP TFBS TFBS TFBS


    2.- Organize the parts of your biosensor device
    3.- Prototype the function of your biosensor device (1)
    3.- Prototype the function of your biosensor device (2)

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