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Analytical Biochemistry

Book · January 2013

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Safwan Ashour
Gaziantep University
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ANALYTICAL BIOCHEMISTRY

S. ASHOUR
R. ALKHLIL

2014
 Content
Preface 9
Chapter One
EXPRESSIONS OF ANALYSIS RESULTS
1.1 Solid Samples 11
1.2 Liquid Samples 13
Problems 17
Chapter Two
STATISTICAL ANALYSIS OF RESULTS
2.1 Significant Figures 21
2.2 Rounding Off 25
2.3 Accuracy and Precision 26
2.4 Determinate Errors 26
2.5 Indeterminate Errors 28
2.6 Ways of Expressing Accuracy 29
2.7 Ways of Expressing Precision 29
2.8 The Confidence Limit 34
2.9 Test of Significance 36
2.10 Linear Least Squares 41
2.11 The Correlation Coefficient 42
2.12 Quality Control and Quality Assurance 44
Chapter Three
KINETICS METHODS OF ANALYSIS
3.1 Kinetics 47
3.2 Catalysis 50
3.3 Enzyme Catalysis 54
Chapter Four
SPECTROPHOTOMETRIC METHODS
4.1 Absorption Spectrophotometry 65
4.2 Electronic Spectra and Molecular Structure 73
4.3 Derivatives in Spectroscopy 83
4.4 Flame Photometry 92
4.5 Atomic Absorption Spectroscopy 96
Problems 105
Chapter Five
ELECTROCHEMISTRY
5.1 Potentiometry 108
5.2 Amperometry 129
5.3 Calibration and Quality Control of Gas Electrodes: Pco2 and Po2 132
5.4 Coulometry 133
5.5 Conductometry 134
5.6 Biosensors 136
5.7 Measurement of Potential 143
Chapter Six
CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS
6.1 Advantages of the Separation Methods in Clinical Works 151
6.2 Theoretical Aspects of the Separation In Chromatograph 151
6.3 General Mechanisms of Chromatography 156
6.4 Types of Chromatographic Techniques 166
 6.5 Capillary Electrophoretic Techniques 196
Chapter Seven
ELECTROPHORESIS
7.1 Separation Methods Based Primarily on Charge 206
7.2 Separation Methods Based on Molecular Size and Shape 222
7.3 Detection Methods 224
7.4 Forces That Cause the Acceleration of Proteins 227
7.5 Polyacrylamide Disc Electrophoresis 230
Chapter Eight
AN INTRODUCTION TO CLINICAL LABORATORY IMMUNODIAGNOSTICS
8.1 Uses of Immunoassays 235
8.2 Components of Immunoassays 238
Antibodies 238
Labels 243
Standards, Calibrators and Controls 250
8.3 Assay Formats 252
Competitive and Sandwich Assays 253
Heterogeneous Assays 256
Homogeneous Assays 259
8.4 Assay Systems 263
Manual Microtiter Plate Assays 263
Semiautomated Fluorescence Polarization Immunoassay 265
Chapter Nine
IMMUNOASSAY
A PRESPECTIVE FROM THE CLINICAL LABORATORY
9.1 Background 268
9.2 Radioimmunoassays 274
9.3 Enzyme Immunoassays 284
9.4 Fluorescence Immunoassays 287
9.5 Chemiluminescenct Immunoassay 288
9.6 Nonlabeled Immunoassay 296
Chapter Ten
CLINICAL CHEMISTRY
10.1 The Composition of Blood 297
10.2 Collection and Preservation of Samples 312
10.3 Clinical Analysis 314
10.4 Blood Gas Analyzer 317
10.5 Van Slyk Apparatus 317
10.6 Trace Elements in the Body 319
Chapter Eleven
DRUGS ANALYSIS
NARCOTICS AND DANGEROUS DRUGS
11.1 Classification of Drugs 337
11.2 Gas Chromatogrphy Screening 341
11.3 Thin-Layer Chromatogrphy Screening 346
11.4 Spectrophotometric Measurement Methods 350
References 353
Appendix 355

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