EFFECTENZTM M 1000

TREATING REAL LIFE STAINS,

PROVIDING REAL VALUE
INTRODUCING EFFECTENZ™ M 1000 THE RESULTS
We at DuPont are committed to develop EFFECTENZ™ M 1000 delivers visibly EFFECTENZ™
products that meet consumer needs. comparable results to premium priced Cleaner clothes,
That’s why the consumer is at the heart mannanase, helping you supply consumers better value.
of our innovation. with the results they are looking for – for
less. Fabric vibrancy is protected, by targeting
Look no further than the
The best products in a cost conscious mannan gum resulting in a detergent with
market offer value without compromising long-term deep-clean benefits. EFFECTENZ™ laundry
on performance. Consumers are consistently product family to meet
exposed to gum based stains, due to the Adding EFFECTENZ™ M 1000 to your consumer needs for
prevalent use of mannans in convenience detergent formula provides deep-clean performance yet value.
foods. The challenge in consumer laundry benefits at 30˚C; enabling you to deliver on Price sensitive cleaning
therefore is tackling these stains whilst the sustainable washing needs of consumers solutions to give you
providing value. without compromising on performance. cost containment –
without compromising
EFFECTENZ™ M 1000 has been created DELIVERING VALUE on performance.
to enable the detergent manufacturers to Through our commitment to innovation
respond to the consumer demand for an we have created an enzyme ingredient to
effective detergent at an affordable cost. help you meet changing consumer needs.
EFFECTENZ™ M 1000 fits perfectly into the
A CLOSER LOOK AT PERFORMANCE EFFECTENZ™ family as a value based
EFFECTENZ™ M 1000 is a granular mannan stain treatment. Offering a functional
mannanase for laundry applications, addition to existing detergents or a viable
designed for integration alongside amylases. alternative to premium mannanase.
Performance goes beyond the stains that
are visible. EFFECTENZ™ M 1000 removes WHAT’S NEXT?
invisible gum-based residue, providing a Because we put the consumer’s needs at
deep-clean effect. the heart of our innovation and collaboration,
we help to ensure detergents make a
EFFECTENZ™ M 1000 remains effective in difference across the globe. For more
a wide spectrum of wash conditions including information, speak to your representative
temperature, cycle time, water hardness and directly or visit www.biosciences.dupont.com
in presence or absence of bleach. Making Alternatively, send us an email:
it a functional addition or viable alternative fabricandhouseholdcare@dupont.com
to premium mannanase products for
detergent formulae.

CS-73 (LOCUST BEAN GUM): EFFECTENZ™ M 1000 VS COMPETITIVE MANNANASE IN

COMMERCIAL DETERGENT
Fig.1: Statistical Performance Fig.2: Visible Perception

80%

75%

70%
Avg (% SRI(dE))

65%

60%
EFFECTENZ™ M 1000
55%
Competitive mannanase
50%

45%
without with 0.15% with 0.15%
40% mannanase EFFECTENZ™ Competitive
0 0.05 0.1 0.15
M 1000 mannanase
Mannanase dose (% w/w)

Fig.1 & Fig.2: EFFECTENZ™ M 1000 matches competitor mannanase cleaning performance.
Machine type: Miele PW 6065, Wash cycle: Universal Cotton cycle, Detergent dosage: 7.63 g/liter, Water hardness: 250ppm, Temperature: 30oC.
 TOGETHER, WE CAN INSPIRE CLEANER IDEAS.

WHAT’S NEXT?
For more information, speak to your representative directly or visit:
fhc.biosciences.dupont.com

Alternatively, send us an email:

fabricandhouseholdcare@dupont.com

Copyright © 2014 DuPont. All rights reserved.

, DuPont™, EFFECTENZ™ M 1000, and all products with ® or ™ are registered trademarks or trademarks of E. I. du Pont de Nemours and Company or its affiliates.
EFFECTENZ™ P 1000
Protease Enzyme for Granular Detergents

INTRODUCTION 120

100
EFFECTENZ™ P 1000 enzyme is a low-dust, granular alkaline
protease for use in the formulation of detergent products. 80

% Relative Activity
EFFECTENZ™ P 1000 protease hydrolyzes insoluble protein
60
stains into soluble peptides and amino acids which then can be
removed easily from fabric. It is useful in removing common 40
household stains such as blood, grass, milk, and gravy.
20

EFFECTENZ™ P 1000 protease is a high-efficiency enzyme 0

6 7 8 9 10 11 12
derived from a genetically modified Bacillus subtilis strain. pH
The product works in conjunction with surfactants, builders,
and other laundry chemicals to enhance the removal of most Figure 1:
EFFECTENZ™ P 1000 pH Activity Profile
stains from all types of cloth surfaces under a wide variety of Assay temperature: 25°C
conditions.

ENZYME PROPERTIES
TYPICAL CHARACTERISTICS
EFFECTENZ™ P 1000 enzyme is active over a broad range of
pH values from 7 - 12, as shown in Figure 1. Enzyme activities Activity: 4000E Minimum 38,000 GSU/kg
are normalized to pH 10 where activity is 100%. The product Appearance: Off-white, smooth granules
is diluted into a buffer and then assayed at 25°C. Enzyme
Bulk density: 0.8 - 1.0 g/ml
activities at temperatures from 25° - 65°C at a pH of 8.6 are
depicted in Figure 2. All activities are normalized to 100% Granule size: 50 - 20 mesh (0.3 - 0.85 mm
activity at 40°C. In solution, EFFECTENZ™ P 1000 enzyme
is stable at 25°C for 24 hours over a broad range of pH values EFFECTENZ™ P 1000 protease contains no other significant
from 5 - 11, as shown in Figure 3. Thermal stability of diluted enzyme activities or proteins.
EFFECTENZ™ P 1000 enzyme after a 10 minute incubation
in a buffer solution at pH 10 is depicted in Figure 4.
250
UNITS OF ACTIVITY
200
% Relative Activity

The activity of EFFECTENZ™ P 1000 enzyme is expressed 150

in Genencor Subtilisin Units (GSU) per kilogram. Enzyme
activity is determined spectrophotometrically against an 100

internal standard using a peptide substrate. The assay method 50

is availableupon request.
0
25 30 35 40 45 50 55 60 65
Temperature (°C)

Figure 2:
EFFECTENZ™ P 1000 Temperature Activity Profile
Assay pH: 8.6
 120 SAFETY & ENZYME HANDLING
100 Inhalation of dust from granulated enzymes should be
80
avoided. In case of accidental spillage or contact with the skin
% Relative Activity

or eyes, promptly rinse with water for at least 15 minutes.

60
For detailed handling information, please refer to the
40
appropriate Material Safety Data Sheet, the Enzyme
20 Technical Association (ETA) handbook Working Safely
With Enzymes, and the Association of Manufacturers of
0
6 7 8 9 10 11 Fermentation Enzyme Products (Amfep) handbook Guide
pH
to the Safe Handling of Microbial Enzyme Preparations, and
Figure 3: the Soap and Detergent Association (SDA) handbook Work
EFFECTENZ™ P 1000 pH Stability Profile Practices for Handling Enzymes in the Detergent Industry.
24 hours at 25°C

100

80
% Residual Activity

60

40

20

0
25 30 35 40 45 50 55 60 65
Temperature (°C)

Figure 4:
EFFECTENZ™ P 1000 Temperature Stability Profile
10 minutes at pH 10.0

For more information, speak to a member of the Fabric &

USAGE IN DETERGENT FORMULATIONS
The recommended usage of EFFECTENZ™ P 1000 enzyme
is 0.1 - 0.5 DSU/liter of wash liquor. Determination of
the exact amount of EFFECTENZ™ P 1000 required for
laundry applications should be based upon wash conditions,
detergent formulae, amount of detergent used per wash, and
the level of cleaning performance desired.
Household Care team at DuPont Industrial Biosciences.
Website: fhc.biosciences.dupont.com
Email: fabricandhouseholdcare@dupont.com
Copyright © 2012 DuPont. All rights reserved. The DuPont Oval Logo, Genencor®, EFFECTENZ™,
and the Leaf Globe are trademarks or registered trademarks of E. I. du Pont de Nemours and Company
or its affiliates.

Use and/or sale of this product may be covered by one or more patents in the U.S. and other countries
as well as pending patents in the U.S. and other countries.
PACKAGING
The information provided herein is based on data DuPont believes to be reliable, to the best of its
knowledge, applies only to the specific material designated herein as sold by DuPont and does not
EFFECTENZ™ P 1000 enzyme is available in various apply to use of the material in any process or in combination with any other material. It is provided
package sizes. at the request of and without charge to our customers. Accordingly, DuPont cannot guarantee or
warrant such information and assumes no liability for its use. Other than as set forth in a contract of
sale, DuPont makes no warranty, express or implied, as to the material set forth herein, including the
warranty of merchantability or fitness for a particular use.
STORAGE & STABILITY In case this product literature is a translated version of the original and internally approved English
version, DuPont hereby disclaims responsibility for any editorial changes and errors caused by the
EFFECTENZ™ P 1000 enzyme is formulated for long-term translation, and shall not be liable for any losses caused by reliance on the accuracy and reliability of
translated information. The original and internally approved English version is and shall remain the
storage. To retain maximum enzyme activity, this product controlling version of this document.

should be stored at moderate temperatures in the original This document is subject to change without further notice.
containers with the lid closed. REV 01 20FEB2013 EFF P 1000
 PREFERENZ TM S 1000
PREMIUM CLEANING ON A WIDE
RANGE OF STUBBORN STARCH STAINS.
INTRODUCING PREFERENZ™ S 1000
The Fabric & Household Care team at
DuPont is committed to developing
cleaning solutions that help you meet the
needs of your consumers.
As food manufacturers develop healthier
products that contain less fat, thickening
agents – like starch – are being used
as a replacement. This results in more
processed foods containing modified starch
and starch-based stains on the clothes of
consumers, leading to changes in their
laundry needs.
THE CHOICE IN CLEANING PERFORMANCE
PREFERENZ™ S 1000 is a premium granular
amylase for use in laundry applications.
It is designed to expand cleaning
performance on stubborn starch stains and
is effective against all types of starch often
found in fresh and processed foods.
Detergent formulation needs differ by
brand. That’s why PREFERENZ™ S 1000
has been designed for use in regular and
concentrated powder detergents, as well
as in tablets. PREFERENZ™ S 1000 can
easily be integrated into a formulation as a
single or multi-enzyme ingredient and also
offers a high level of stability – even under
stressed conditions.
The Fabric and Household Care team can
help to optimize your detergents with
PREFERENZ™ S 1000.
PREFERENZ™ S 1000 SOIL REMOVAL PERFORMANCE ON RICE STARCH (40°C)
80%
70%
60%
50%
% SR
40%
30%
20%
10%
0%
0% 0.1% 0.2% 0.3%
% w/w
THE RESULTS
PREFERENZ™ S 1000 outperforms other PREFERENZ™
premium starch-removing enzymes in the Preferred stain removal
market. It delivers stain-busting cleaning technology for laundry.
performance on stubborn starch-based soils
like rice and corn starches, in bleach and Consumers have high
expectations when
non-bleach detergents, at a wide range of
it comes to laundry,
washing temperatures – including Europe’s
expecting clean
favorite: 40°C.
clothes first time,
PREFERENZ™ S 1000 – is a great alternative irrespective of stain or
to your regular premium amylase. cleaning condition. The
PREFERENZ™ product
DELIVERING CONVENIENCE family leverages premium
Through continued research and thorough enzyme technology to
testing, Fabric & Household Care offers a deliver optimum laundry
premium amylase for laundry detergents performance.
that addresses the need for convenience
and premium cleaning performance.
PREFERENZ™ S 1000 ensures a great
first-time clean on the most stubborn
starch-based household stains – across a
range of washing conditions.
What’s Next?
Because we put the consumer’s needs
at the heart of our innovation and
collaboration, we help to ensure detergents
make a difference across the globe.
For more information, speak to your
representative directly or visit:
fhc.biosciences.dupont.com
Alternatively, send us an email:
fabricandhouseholdcare@dupont.com
PREFERENZ™ S 1000
Other commercial enzyme
Washing conditions:
40°C, benchscale
Detergent: ECE-2 + bleach (15% Percarbonate + 2.5% TAED)
Water hardness: 20°FH
Stain: Rice Starch (CS-28)
0.4%
 FABRIC & HOUSEHOLD CARE
TOGETHER, WE CAN INSPIRE CLEANER IDEAS.

WHAT’S NEXT?
For more information, speak to your representative directly or visit:
fhc.biosciences.dupont.com

Alternatively, send us an email:

fabricandhouseholdcare@dupont.com

Copyright © 2013 DuPont. All rights reserved.

, DuPont™, PREFERENZ™ S 1000, and all products with ® or ™ are registered trademarks or trademarks of E. I. du Pont de Nemours and Company or its affiliates.