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Answer all questions. Write the answers on the answer sheets provided.
(c) Name the reaction that breaks down polysaccharides into simple sugars. [1]
Hydrolysis
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X Y Z
(a) Classify amino acids X, Y and Z based on their side chains. [3]
X : Basic amino acid
Y : Polar amino acid
Z : Non-polar amino acid
(b) Identify two functional groups of an amino acid and state their characteristics.
[2]
Functional group Characteristic
Amino group Basic
Carboxyl group Acidic
(c) Name the bond that joins two amino acids together. [1]
Peptide bond
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Answer all questions. Write the answers on the answer sheets provided. Begin each answer on
a new page of the answer sheet.
(b) Describe the structure of DNA based on Watson and Crick model. [7]
- DNA consists of two polynucleotide strands
- the two strands twist around each other, forming a double helix
- the two strands are antiparallel, one strand runs from 5’ to 3’ while the
other strand runs from 3’ to 5’
- each strand consists of a sequence of nucleotides linked together by
phosphodiester bonds
- each strand has a sugar-phosphate backbone made of alternating
deoxyribose and phosphate groups
- the two strands are held together by hydrogen bonds between the
complementary base pairs
- adenine pairs with thymine by two hydrogen bonds
- guanine pairs with cytosine by three hydrogen bonds
- one complete turn of the double helix consists of ten base pairs
- the distance between two base pairs is 3.4 Å
(Any 7)
(c) Explain the principles of protein separation using gel electrophoresis. [5]
- polyacrylamide gel electrophoresis is used to separate proteins
- involves the movement of proteins through the gel under the influence
of electric field
- smaller proteins move faster
- negatively charged proteins move towards the anode// positively
charged proteins move towards cathode
- highly charged molecules move faster
- larger pore size of gel allows molecules to move faster
- higher voltage supplied to the gel causes proteins to move faster
(Any 5)
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