Reviewer Comments:

Reviewer 1

1. in lines 129, describe the most important perineal patterns of this species, relevant
characteristics of the male and juveniles.

The accepted methods for identifying root-knot nematodes perineal patterns

Second-stage juveniles of root-knot nematodes

Many species overlap in body length; therefore, this character is inadequate for
species identification. Due to the small size of the second-stage juvenile, it is difficult
to discern the head morphology precisely. Second-stage juveniles have the same basic
head characteristics as males, but they can be seen clearly only with the scanning
electron microscope (SEM). In general, juveniles are quite similar among species and
therefore may be difficult to use precisely for species identification for root-knot
.nematodes. (Eisenback, 1985)

Male of root-knot nematodes

Root-knot nematodes reproduce asexually, and males are rare in Egypt. When
conditions are unfavorable, such as when there is not enough food or a suitable host,
all females transform into males because they are fed, in what is known as the
phenomenon of intersex .for this cannot use males for identified root-knot nematodes

Eisenback, J.D. Detailed morphology and anatomy of second-stage juveniles, males,

and females of the genus Meloidogyne (root-knot nematodes). In An Advanced
Treatise on Meloidogyne; Sasser, J.N., Carter, C.C., Eds.; State University Graphics
Raleigh: Raleigh, NC, USA, 1985; Volume I, pp. 47–77.
2. in Lines 100 "environment friendly [11]. In this concern, Artemisia spp. has produced about
1000 ..." Artemisia spp. must be in italics.

3. in section

Statistical analysis
"All data collected were directly subjected to analysis of variance (ANOVA) and significant
means separated with Duncan’s Multiple Range Test (DMRT) at P < 0.05 level using the ..."

the percentages do not meet the tests of normality , therefore they need to be transformed
and thus be analyzed, which will give a more accurate result than what they are reporting.

The mortality values are equal to the original values as there is no mortality in the control. The
percentage of mortality is largely used to appearing the effect of treatments in the lab.

4. lines 296-7

"Nineteen phenolic and flavonoids compounds from 20 tested compounds were identified in
A. annua aqueous..." OK, I suggest summarizing in this way, because the nature of these
standard compounds has already been described in  "materials and methods section":
"Nineteen compounds were identified from 20 standards  tested  in A. annua aqueous ..."

5. it is suggested to review the style and language... in some parts of the text. I agree that the
research carried out by the authors is original and this type of review will support its
dissemination and contribution.

Reviewer 2
After revision, the manuscript has been greatly improved, but there are still some details that
need to be revised. My comments are as follows:

1. Line 436 and 440: Correct “ Preicarp” to “Pericarp”

2. Fig. 3: Please add the picture of root galls in M. incognita infected tomato roots from
samples A250, A125, NA250, NA125, L250, L125, NL250, and NL125. 

There is difficulty in photographing the infected roots at different concentrations as the

differences among the different concentrations are too small to be evident. The number of
photos would be large, which leads to confusion and failure to clarify the differences in the
effect of the treatments. So the highest concentrations were adopted to clarify the differences
between the treatments. The infected roots were photographed while they were fresh, and we
do not have these roots saved in formaldehyde for photographing them.

After these revisions, the manuscript can be accepted for publishing.

"