MKBN 211

Antibiotikum vatbaarheidstoetsing

MKBN211
Antibiotic susceptibility testing
 Studie materiaal / Study material

• Praktiese handleiding / Practical Manual:

– Eksperiment 26 / Experiment 26
– Bladsy 81 – 82 / Page 80 – 81
• Prescott 10
– Hoofstuk 9 / Chapter 9
– Afdeling 9.2; 9.3; 9.4 / Section 9.2; 9.3; 9.4
– Bladsye 190 – 199 / Pages 190 – 199
Antibiotikum vatbaarheidstoetsing /
Antibiotic susceptibility testing
 Inleiding / Introduction

• Verskeie metodes • Various methods

– Verdunningvatbaarheids – Dilution susceptibility
-toets vir MIK test for MIC
• MIK vs MLK? • MIC vs MLC?
– Skyfdiffusietoetse – Disk diffusion test
(Kirby-Bauer) (Kirby-Bauer)
– E-toets MIK en diffusie – E-Test MIC and
diffusion
 Inleiding / Introduction

• Skyfie diffusie toets • Disk diffusion test

– Kirby-Bauer toets
– Metode vir hierdie
eksperiment
– Mat van mikro-
organisme
– Op Mueller-Hinton agar
– Skyfie bevat antibiotika
– Stel bloot en neem
metings
– Kirby-Bauer test
– Method for this
experiment
– Lawn of micro-
organism of interest
– On Mueller-Hinton agar
– Disk with antibiotic
– Expose and take
measurement
Metode / Method
Metode / Method

Measure diameter of
inhibition zone in
mm
 Interpretasie van die resultate /
Interpreting the results
• Voorbeeld van • Example of results
resultate

Bacteria 1
Inhibition zone (mm)
Erythromycin (E 15) 18
Gentamycin (CN 10) 23
Ampicillin (AMP 10) 13
Kanamycin (K 30) 24
 Interpretasie van die resultate /
Interpreting the results
• Vergelyk met tabel • Compare to table
 Interpretasie van die resultate /
Interpreting the results
• Voorbeeld van • Example of results
resultate – Compare diameter to
– Vergelyk deursnee antibiotic in table
met antibiotikum in – Determine
tabel – S – Susceptible
– Bepaal: – I – Intermediate
– S – Vatbaar – R – Resistant
– I – Intermediêr
– R – Weerstandig
– Pay attention to
– Let op na Ampicillin Ampicillin
 s s ss

s
s

Bacteria 1 Inhibition zone (mm) Result

Erythromycin (E 15) 18 I
Gentamycin (CN 10) 23s S
Ampicillin (AMP 10) 13s R
Kanamycin (K 30) 24s S
Eksperimentele werk /
Experimental work
 Eksperimenteel / Experimental

• Kulture gebruik / Cultures used:

– Escherichia coli
– Staphylococcus aureus
– Bacillus subtilis
• Antibiotika gebruik: / Antibiotic used:
– Trimethoprim (W)
– Ampicillin (AMP)
– Vancomycin (VA)
– Chloramphenicol (C)
 Resultate / Results

• Beskikbaar in XLS dokument /

Available in XLS document:
• Bereken gemiddeld van die 4 metings per
antibiotika en per mikro-organisme /
Calculate average of the 4 measurements
per antibiotic and micro-organism
• Eindig met tabel met 3 mikro-organismes
met elk 4 antibiotikum resultate /
Ends with table with 4 micro-organisms
with 4 antibiotic results each
 Resultate / Results

• Vergelyk met Tabel 26.1 en klassifiseer as

S, I of R /
Compare to Table 26.1 and classify as S, I
or R