Microtomy Prepared by: Ms.

BR Tsauses
Paraffin & frozen sections Anatomical Pathology 2A
(ANP611S)
Lecture 5 (Practical lecture) March 2022
 • To demonstrate practical skills to be able
Objectives to troubleshoot during and after
sectioning.
• Describe the techniques necessary to
provide quality microscope slides for
clinical and research histology.
Troubleshooting
Troubleshooting
Troubleshooting
Troubleshooting
Troubleshooting
Troubleshooting
 Familiarize yourselves with the contents of
Practical 3: the practical manual and ensure that you
Microtomy are well prepared for the practical.
This practical lecture slides will help you,
combined with the theoretical lectures.
Good luck and enjoy the practical.
Group 1: Monday
Group 2: Tuesday
Be punctual!!!
Questions???
 •Our next lecture
will be on
Principles and
Next lecture Theory of
Histological Stains:
H&E Stains.
 • Suvarna, S. K., Layton, C., Bancroft, J.D. 2013.
Bancroft’s Theory and practice of Histological
techniques, Expert consult . 7th Edition. Churchill
Livingstone, Elsevier.
• Leica Microsystems. Instruction Manual Leica
References RM2235 V1.3 Nussloch: Leica Microsystems, 2008.
• Rolls GO, Farmer NJ, Hall JB. Artifacts in Histological
and Cytological Preparations. Melbourne: Leica
Microsystems, 2008;106.
• Rolls G. 101 Steps to Better Histology. Melbourne:
Leica Microsystems, 2008.
• Culling CFA, Allison RT, Barr WT. Cellular Pathology
Technique. 4th ed. London: Butterworths, 1985.
• Santoianni RA, Hammami A. Nuclear Bubbling an
Overlooked Artifact. The Journal of Histotechnology,
1990;13;135-136.