Modul 1

INTRODUCTION: Pipetting and Reagent Kit Preparation

a. Pipetting
Volume of pipetting: 1 mL

Water mass (ml) Mean SD KV

Micropipette 1.0056
(100-1000 µl) 1.0078
1.0054
1.0045
1.0098
0.9631
0.9762
Measuring 0.9731
pipette 2 ml 0.9698
0.9701

Volumetric 0.9586
pipette 1 ml 0.9620
0.9582
0.9593
0.9659
1. Calculate the accuracy and precision of each pipette!
2. Which micropipette is right to use? Why?
3. Is it important to use the appropriate pipette in piping the solution? Why?

b. Reagent kit preparation and spectrophomoteter UV-Vis

Blank absorbance = 0.05
tion

%Recovery
Nilai nyata 1
1.0066 0.002152 0.002138
Zn Concentration (mg/L) Absorbance 1 Absorbance 2
2 0.201 0.202
4 0.269 0.271
6 0.363 0.363
8 0.455 0.456
10 0.574 0.575
12 0.688 0.689
Sample A 0.601 0.602
Sample B 0.255 0.255
1. Explain reaction between Zn and XO!
2. What is the function of reagents used?
3. Why we use spectrofotometer UV-Vis for this experiment?
4. Write down the steps using UV-Vis Spectrophotometer Instrument!
5. Calculate correlation coefficient, LOD, and LOQ!
6. Calculate the mass of ZnO in sample if the preparation:
a. Sample A in aquadest 100 mL, take 1 mL add to 25 mL
b. Sample B in aquadest 50 mL, take 1 mL add to 10 mL
Absorbance 3
0.203
0.271
0.364 Nilai nyata 1
0.456 0.536 0.127733 0.238219
0.576
0.689 buat tabel lagi dikurangi blanko
0.603
0.255