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Protozoans

Characteristics Unicellular
Animal-like protists
Division: Fission
No cell wall w/ outer membrane (periplast) Cytoplasm: ectoplasm (outer) and endoplasm (inner)
Contains at least 1, and some several nuclei
Some contain vacuoles: for storage and transport
Hemotogranules Special organs for locomotion
-Sarcodina/Rhizopoda = Amoeba (pseudopods)
-Mastigophora/Flagellata
-Ciliophora/Ciliata
-Sporozoa = no definite locomotory organelle

Class Rhizopoda

Trophozoites Motile Feeding stage Diarrheic/liquid


stool Destroyed by iodine
Cyst Non-motile Nonfeeding stage
Well-formed stool Infective stage
Characteristics w/ cystic stage except Entamoeba gingivalis
Inhabit the large intestine except Entamoeba gingivalis
Commensals except Entamoeba histolytica
Life cycle Trophozoite > Precystic > Cystic > Metacystic stage
Entamoeba True amoeba
Peripheral chromatin (trophozoite and cyst) Chromatoidal
bars (cyst)
Endolimax/Iodamoeba Other amoeba
No peripheral chromatin
No chromatoid bars

Entamoeba

Endolimax nana

Smallest amoeba
Size = RBC
[Trophozoite]
-Karyosome: large round dot
[Cyst]
-Usually oval
-Nuclei: 4 (cross-eyed)
Iodamoeba butschlii ,Iodamoeba williamsi

[Trophozoite]
-Karyosome: w/ cluster of achromatic granules
[Cyst]
-Large iodine-staining glycogen vacuole

Entamoeba gingivalis

Associated w/ gum infection


[Trophozoite]
Karyosome: small and centrally located
Pseudopodia: similar to E. histolytica but does not exhibit true progressive locomotion

Opportunistic Amoeba

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