Reactions of Proteins solid by altering the protein

solubility with the addition of a

Proteins reagent to eliminate
interferences or purify them.
 are the most abundant organic
molecules (carbon containing) in the Different ways of precipitation:
living system. They offer structural and
dynamic function. A. Precipitation by salt
 polymers of amino acids that were Principle: Neutral salt like ammonium
linked by covalent peptide bonds. sulfate precipitate proteins by salting
 used mainly for protein synthesis as out which involves the removal of the
dictated by the genes of respective shell hydration causing precipitation of
tissues (differential expression). proteins. Higher the molecular weight
 Proteins are present in all types of body lesser the salt will be required for
fluids. precipitation.
Classification of proteins B. Precipitation by heavy metals
1. Fibrous proteins C. Precipitation by an ionic reagents or
alkaloids
 Contain polypeptide chains
D. Precipitation by organic solvents
organized approximately in
E. Precipitation by heat
parallel long axis, producing
F. Precipitation by strong mineral acids
long fibers or large sheets.
2. Color reactions
 Examples are fibrinogen,
troponin, collagen, myosin
2. Globular proteins
 A protein that is water soluble
and shaped like a sphere or a
globe upon folding.
 Referred to as sphero-proteins
due to their spherical shape.
 Examples are enzymes, peptide
hormones, plasma proteins.
3. Derived proteins
 Proteins that are obtained
through hydrolysis.
 Examples are casein from
skimmed milk, hemoglobin
from meat, gluten from wheat
flour.

Ways to study Proteins

1. Precipitation reaction
 The process of separating a
protein from a solution as a