Name: kelompok 5 activiti 3

Exercise 2: Skeletal Muscle Physiology: Activity 3: The Effect of Stimulus Frequency on Skeletal
Muscle Contraction Lab

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Pre-lab Quiz Results

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Experiment Results Predict Question:

Predict Question 1: As the stimulus frequency increases, what will happen to the muscle force
generated with each successive stimulus? Will there be a limit to this response?

Your answer : d. As the stimulus frequency increases, the muscle force generated by each successive
stimulus will first increase and then decrease as the stimulus frequency becomes very high.

Predict Question 2: In order to produce sustained muscle contractions with an active force value of
5.2 grams, do you think you will need to increase the stimulus voltage? Your answer : b. no

Stop & Think Questions:

Was there any change in the force generated by the muscle during the second stimulated twitch?

Voltage Length Stimulus Active Force Passive Force Total Force

8.5 75 Single 1.83 0.0 1.83

8.5 75 Single 2.40 0.0 2.40

8.5 75 Multiple 3.38 0.0 3.38

8.5 75 Multiple 5.01 0.0 5.01

10 75 Multiple 4.78 0.0 4.78

8.5 75 Multiple 5.02 0.0 5.02

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Correct answer: c. Yes, the second twitch generated more muscle force.

Is the total muscle force generated by the higher frequency stimulation greater than the force
generated in previous stimulations?

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Correct answer: a. Yes, it is greater than the previous stimulations.

Does the force generated by the muscle change with each additional stimulus?

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Correct answer: b. As the stimulus frequency increased, the muscle tension generated by each
successive stimulus also increased, and a limiting maximum value was observed.

Post-lab Quiz Results

You scored 75% by answering 3 out of 4 questions correctly.

1. Which of the following is not one of the ways that the body can increase the force produced
by a skeletal muscle?

Your answer: b. application of high-frequency stimulation by a motor neuron

Correct answer: d. application of higher voltages to the whole muscle

2. When a muscle receives a stimulus frequency that causes non-overlapping twitches to follow
each other closely in time such that the peak tension of each twitch rises in a stepwise fashion up to
a plateau value, the result is known as You correctly answered: c. treppe.

3. In this experiment the isolated skeletal muscle was repetitively stimulated such that
individual twitches overlapped witheach other and resulted in a stronger muscle contraction than a
standalone twitch. This phenomenon is known as You correctly answered: c. wave summation.

4. Wave summation is achieved by

You correctly answered: a. increasing the rate of stimulus delivery (frequency) to the muscle.

Review Sheet Results

1. What is the difference between stimulus intensity and stimulus frequency?

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2. In this experiment you observed the effect of stimulating the isolated skeletal muscle
multiple times in a short period withcomplete relaxation between the stimuli. Describe the force of
contraction with each subsequent stimulus. Are these results called treppe or wave summation?

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3. How did the frequency of stimulation affect the amount of force generated by the isolated
skeletal muscle when thefrequency of stimulation was increased such that the muscle twitches did
not fully relax between subsequent stimuli? Are these results called treppe or wave summation?
How well did the results compare with your prediction?

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4. To achieve an active force of 5.2 g, did you have to increase the stimulus voltage above 8.5
volts? If not, how did youachieve an active force of 5.2 g? How well did the results compare with
your prediction?

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5. Compare and contrast frequency-dependent wave summation with motor unit recruitment
(previously observed byincreasing the stimulus voltage). How are they similar? How was each
achieved in the experiment? Explain how each is achieved in vivo.

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