91195 Prac Slides

Practical 1 (week 2)
Hand Sanitiser Results

Faculty of Science
 FACULTY OF SCIENCE 2

Discussion Points hand Sanitiser Prac

Most people had at least 3 and often 4-5 types of bacteria on their hands, at
time 0.

This included Staph epidermidis, Staph aureus, micrococcus, Streps, Bacillus,

E.coli(maybe)

We saw a few fungus contaminations

To identify the bacteria more fully, we could plate the organisms onto
other types of differential and selective media.
Do more biochemical tests. PCR and Sequencing

We saw a big range of numbers at time 0

From 0 to ‘too many to count’
 FACULTY OF SCIENCE 3

Discussion Points hand Sanitiser Prac

Why this difference in initial cell number?

• Some students said they sanitised their hands coming into class,
this could have reduced their initial reading
• We did not time how long they all swabbed their hands
• How wet the swabs where
• Did they all swab under their fingernails and between the fingers

Overall the numbers of bacteria fell from time 0 to time 5 mins and
then started to increase by 60 mins
There were some outliers- with some seeing a fall at 60 mins but not
at 5 mins.

By 5 and 60mins the number of different colony types on the plates

had fallen. Mostly this was staph epidermidis.
 FACULTY OF SCIENCE 4

Hand Sanitiser Data- comparison

All sanitisers combined (Mean+SEM)
✱✱✱✱ Statistics:
One-way Anova
ns Repeated measures, Comparing the
mean of every group to every other
1000
✱✱✱✱ group
Tukey's multiple comparisons test
800
Bacterial counts

600 Anyone who wrote ‘too many to count’

A value of 800 was used.

400 **** p < 0.001

200

0
0 5 60
TIme (mins)
 FACULTY OF SCIENCE 5

Hand Sanitiser Data- individual sanitisers

Dettol
SOAP ✱✱✱ Organic UTS Sanitiser
ns ✱✱ ✱
ns

ns ns ✱✱✱ ns ✱ ns
1000 1000
✱✱✱✱ 1000 1000
800 800 800 800
Bacterial counts

Bacterial counts
600
Bacterial counts

Bacterial counts
600 600 600
400
300
400 400 400
200
200 200 200
100

0 0 0 0
0 5 60 0 5 60 0 5 60 0 5 60
TIme (mins) TIme (mins) TIme (mins) TIme (mins)

**** p < 0.001, ** p < 0.01 * p < 0.05

FACULTY OF SCIENCE 6

Hand Sanitiser Data-

All combined
1000 Each line represents an individual
students data at time 0, 5, and 60
750
Bacterial counts

500
250

200

100

0
0 5 60
TIme (mins)
 FACULTY OF SCIENCE 7

Hand Sanitiser Data- Dettol Data presented four

ways Dettol Dettol
✱✱✱✱
Dettol
✱✱✱✱
Dettol
✱✱✱
✱✱✱

ns ns ns ns

✱✱✱✱ ✱✱✱✱ ✱✱✱✱ ✱✱✱✱

1000 200 200 1000
800 750
Bacterial counts

Bacterial counts
150 150 500
600

% change
% change

250
100 100
400 100
80
50 50 60
200
40
20
0 0 0 0
0 5 60 0 5 60

%
%

%
%
%

in
0

in
in
0

in

TIme (mins) TIme (mins)

m
m
m
m

60
5

60

Bacterial Counts TIme (mins) TIme (mins)

% Change. 0 is set to 100%

In 2- showing Median with 95% confidence
intervals, in 3- is mean and SEM
 FACULTY OF SCIENCE 8

Hand Sanitiser Data- all data combined presented

four ways
All sanitisers combined All combined (median +
(Mean+SEM) 95% Confidence Intervals)
All combined
✱✱✱✱ All combined
(mean and SEM) 1000
ns 10000
10000 750
5000

Bacterial counts
✱✱✱✱
1000 5000
500
800
Bacterial counts

% change
600 200 250
100
150
400
200
100
50
200 100
50

0 0 0 0
0 5 60
0 5 60

%
%

%
%
%

TIme (mins)

in
0

in
in
0

in

TIme (mins)

m
m

60
5

60

TIme (mins)

Bacterial Counts % Change. 0 is set to 100%

In 2- showing Median with 95% confidence
intervals, in 3- is mean and SEM