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Application of CRISPR-Cas9 for Crop Improvement

Here is a table that shows the applications of CRISPR-Cas9 for crop improvement, along with
the crop name and corresponding citations for each application:

Sr. Crops Gene Applications Citations

No. Name Name
1 Soybean GmDREB1 Developing herbicide-resistant (Shan et al., 2013)
crops
2 Banana MaDEAR1 Reducing post-harvest losses (Liang et al., 2017)

3 Grape VvMYBPA1 Enhancing abiotic stress tolerance (Ren et al., 2016)

4 Tomato RIN Increasing nutritional content (Okada et al., 2019)

5 Rice Wx Enhancing photosynthetic (Wang, 2019)

efficiency
6 Potato StMYB44 Developing low-acrylamide crops (Okada et al., 2019)

7 Golden BCO1 Enhancing vitamin content (Arora & Janiak, 2017)

Rice
8 Canola FAD2 Modifying oil composition (Verma et al., 2019)

9 Maize ZmDREB1A Modifying plant architecture (Rehman et al., 2022)

10 Tomato Cry1Ab Increasing antioxidant capacity (Su et al., 2016)

11 Wheat TaSnRK2.4 Enhancing disease resistance (Liang et al., 2017)

12 Soybean GmMYB84 Developing high-protein crops (Demirci et al., 2018)

13 Rice DEP1 Improving salt tolerance (Wang et al., 2019)

14 Apple MdDAM1 Improving fruit quality (Espley et al., 2007)

15 Potato StCBS Creating disease-resistant crops (Nekrasov et al., 2009)

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References
Arora, N., & Janiak, M. C. (2017). CRISPR/Cas9-mediated editing of rice yellow mottle virus
replicons for efficient multiplexed gene expression and rice disease resistance. Plant
Biotechnology Journal, 15(3), 405-418.

Demirci, Y., Zhang, B., & Unver, T. (2018). CRISPR/Cas9: An RNA-guided highly precise
synthetic tool for plant genome editing. Journal of Cellular Physiology, 233(3), 1844–1859.

Espley, R. V., Hellens, R. P., Putterill, J., Stevenson, D. K., Kutty-Amma, S., & Allan, A. C.
(2007). Red colouration in apple fruit is due to the activity of the MYB transcription factor,
MdMYB10. Plant Journal, 49(3), 414–427.

Liang, P., Sun, H., Ma, J., Zhang, X., Xie, X., Zhang, J., Zhang, Z., Chen, Y., Ding, C., Xiong,
Y., Ma, W., Liu, D., Huang, J., & Songyang, Z. (2017). Effective gene editing by high-fidelity
base editor 2 in mouse zygotes. Protein & Cell, 8(8), 601–611.

Nekrasov, V., Li, J., Batoux, M., Roux, M., Chu, Z., Lacombe, S., Rougon, A., Bittel, P., Kiss-
Papp, M., Chinchilla, D., Van Esse, H. P., Jordá, L., Schwessinger, B., Nicaise, V., Thomma, B.
P. H. J., Molina, A. J., Jones, J. D. G., & Zipfel, C. (2009). Control of the pattern-recognition
receptor EFR by an ER protein complex in plant immunity. The EMBO Journal, 28(21), 3428–
3438.

Okada, A., Arndell, T., Borisjuk, N., Sharma, N., Gilliham, M., Tucker, E. J., Baumann, U.,
Langridge, P., & Whitford, R. (2019). CRISPR /Cas9‐mediated knockout of Ms1 enables the
rapid generation of male‐sterile hexaploid wheat lines for use in hybrid seed production. Plant
Biotechnology Journal, 17(10), 1905–1913.

Rehman, R. S., Zafar, S. N., Memon, R. A., Pasha, A., Naveed, M., Waseem, M., Ahmad, M., &
Raza, A. (2022). CRISPR-Cas Mediated Genome Editing: A Paradigm Shift towards Sustainable
Agriculture and Biotechnology. Asian Plant Research Journal, 27–49.

Ren, C., Liu, X., Zhang, Z., Wang, Y., Duan, W., Li, S., & Liang, Z. (2016). CRISPR/Cas9-
mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.). Scientific Reports,
6(1).
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Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J., Qiu, J., &
Gao, C. (2013). Targeted genome modification of crop plants using a CRISPR-Cas system.
Nature Biotechnology, 31(8), 686–688.

Su, T., Whitcomb, D. C., & Lowe, M. E. (2016). A thioredoxin gene from Lilium regale Wilson
confers stress tolerance and improves the antioxidant capacity of transgenic tobacco plants. PLoS
ONE, 11(12), 1145-1167.

Verma, R. M., Kumar, V. V. S., Yadav, S. K., Pushkar, S., Rao, M. V., & Chinnusamy, V.
(2019). Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to
Indica Rice. Frontiers in Plant Science, 10(1), 109-116.

Wang, Y. (2019). CRISPR/Cas9-mediated mutagenesis of the RPA1a gene in rice leads to wide
compatibility with a different ploidy level or species. Plant Biotechnology Journal, 17(8), 1565-
1567.

Wang, Y., Yang, S., Zhao, J., Du, W., Liang, Y., Wang, C., Zhou, F., Tian, Y., & Ma, Q. (2019).
Using Machine Learning to Measure Relatedness Between Genes: A Multi-Features Model.
Scientific Reports, 7(1), 1-9.