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4 mutations in weed (species) ;

5 chance / random / spontaneous (mutations) ;


6 idea that resistant weeds have selective advantage ; [max 4]

(d) social MARK SCHEME


TOPIC increased
18: CLASSIFICATION, BIODIVERSITY
yield / more food / cheaper food ; & CONSERVATION
environmental PAPER 4: M/J2016 – O/N2022
glyphosate, less hazardous than other weed killers / breaks down in soil
or
SECTION A
less fertiliser used (because weed competition reduced) ; [2]

1 18.1.d, 18.2.b, 18.3.f J16/42/5


[Total: 13]

5 (a) 1 mark-release-recapture ; AW
2 detail of trapping ; e.g. live mammal trap
bait with, food / chocolate / peanut butter
3 detail of marking ; e.g. paint / clipping fur / not to have adverse effects
4 time of second trapping detail ; e.g. not too soon or mixing won’t occur /
not too long after as migration may occur
5 detail of calculation ; e.g. Lincoln / Petersen, index
or
population size = number caught / marked, time 1 x no. captured time 2
number of marked individuals recaptured time 2
6 public reports ; e.g. online site / use of reporting app
7 detail of reporting, time frame / areas ; e.g. raccoon spotting week
8 detail of calculating numbers per unit area / use of computer modelling ; [max 3]

(b) (i) Eukarya ; A Eukaryota R eukaryotes [1]

(ii) 1 (cells) have a nucleus ;


2 (cells) contain membrane-bound organelles ; A mitochondria / ER / golgi
3 ribosomes are, large / 22 nm / 80S ;
4 DNA is linear ;
5 histones present ;
6 ref. to cytoskeleton / microtubules / undulipodia / cilia ; [max 3]

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Cambridge International AS/A Level – May/June 2016 9700 42
© Cambridge International Examinations 2016
(c) max 2 for mp1–4
1 may compete with other species for, food / habitat ;
2 may be predators of other species ;
3 may spread disease to other species ;
4 may reduce population sizes / cause extinction of other species ;
5 may spread, disease / rabies, to humans ;
6 may bite humans ; [max 3]

[Total: 10]

2 18.1.d, 18.2.c, 18.3.f, 18.3.e J16/43/5


6 (a) codominance ;
sex linkage ; [2]

(b) (male) CBCBZaZa ; x (female) CSCSWZA ;

(gametes) CBZa CSZA or CSW ;

CBCSZAZa ; CBCSWZa ;
(male, blue, barred) (female, blue, non-barred)

accept other symbols but only with key [5]

(c) blue colour is, heterozygous / CBCS ;


test cross ;
with non-barred female ;
if all offspring barred, must be ZAZA / homozygous ;
if offspring not all barred, must be ZAZa / heterozygous ; [max 3]

[Total: 10]

7 (a) deamination / amine group removed ; A amino / NH2


ammonia / NH3, formed ;
combined with carbon dioxide ;
Page 6 Mark Scheme Syllabus Paper
Cambridge International AS/A Level – May/June 2016 9700 43

5 (a) 1 mark-release-recapture / AW ; A catch, mark, return, catch


A mark-and-recapture
description (max 3)
2 detail of trapping ; e.g. Longworth / Sherman / live / small mammal
3 detail of marking ; e.g. felt tip pen / clipping fur / not to have adverse effects
4 detail of timing of second trapping ; e.g. not too soon or mixing will not occur /
not too long after as migration may occur / after 24 hours / 1 day (any number
of days up to two weeks)
5 detail of calculation ; e.g. Lincoln Index / Petersen index
or number marked time 1 × no. captured time 2
number of marked individuals recaptured time 2
A symbols in equation if key is given [max 4]

(b) glycogen ;
centrioles / centrosomes ;
(may have) cilia / flagella / microvilli ;
no cell wall ;
no, large / central / permanent, vacuole ; A no tonoplast [max 2]

(c) (i) 1 reduce, other organisms’ abundance / biodiversity ; A endanger, rare


species / water voles A causes extinction
2 alter food, chains / webs ;
3 due to predation ;
4 due to competition ;
5 due to spreading disease ;
6 may change habitat ; e.g. create shade, change soil pH
7 may be toxic / threaten human health ; [max 3]

(ii) culling / hunting / trapping ;


contraceptive measures ;
biological control disease agent ; I introduce new mink-eating predator
I biological control alone [max 1]

[Total: 10]
3 18.1.a, 18.2.a, 18.1.f, 18.3.c N16/43/5
6 (a) key to 4 chosen symbols ;
A any two lettered pairs (e.g. E/e and A/a) identified I symbols for wing length
no eyes and black abdomen must be lower case (e, a)
with eyes and striped abdomen must be upper case (E, A)
allow ecf to max 3 if error in symbols

parents genotypes Eeaa × eeAa ;


gametes Ea ea × eA ea ; A each gamete written twice
F2 genotypes Eeaa eeaa EeAa eeAa ; [4]

(b) cross with, homozygous recessive / black no-eyes, fly ;


A double recessive / aaee (or own symbols) / organism showing recessive
characters or phenotype [1]

© Cambridge International Examinations 2016


Page 10 Mark Scheme Syllabus Paper
Cambridge International AS/A Level – October/November 2016 9700 43

Question Answer Mark Guidance

5(a) three from 3 A interactions between living and non-living


ref. to interactions between, biotic and abiotic (components) ; or between, the community / populations of different
species, and the physico-chemical environment
yes
tree supports, many species / community / many interactions ; A idea of, discrete / self-contained / self-sustaining,
group of species / community
energy flow / food web, occurs on one tree ; A description of three-step food chain on tree

no
idea that tree is part of larger ecosystem / interactions occur with organisms outside
tree ;
part of, savannah / larger, energy flow / food web / nutrient recycling ;

5(b)(i) 105 ; 1 A 104.5 / 104

5(b)(ii) genus Acacia ; 2 R genus name with lowercase first letter


species (Acacia) mellifera / nilotica / tortilis ; R species name with uppercase first letter

5(b)(iii) two from 2


sample sizes in Table 5.1
1 are small ; ora idea that they should be the same
2 sample size varies (between the 3 species) ; ora e.g. large sample size reduces effects of anomalies
or large sample size allows anomalies to be
3 anomaly could be, missed / have disproportionate effect ; indentified / either maximum or minimum for A. tortilis
could be an anomaly / high max for A. nilotica could
be an anomaly

4 small(er) / decreased, sample size would be, less representative / less valid / less
accurate ; ora

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© UCLES 2016
Syllabus Paper
Cambridge International AS/A Level – October/November 2016 9700 43

Question Answer Mark Guidance

5(c) three from 3 A knock-on effects e.g. insects (needed to) pollinate
idea of, species, inter-related / interact (in reserve) ; plants that elephants eat / insect competitors stunt
trees so easier for elephants to reach

I elephants eat insects


to conserve one species need to protect whole ecosystem ;
elephants / large animals, eat acacia ;
AVP ; e.g. research raises, profile of / money for, game reserve or presence of
research team deters elephant poachers

Total: 11

Question 4 18.1.b, 18.1.f,Answer


18.2.a Mark J17/41/8
Guidance

6(a)(i) C; 1

6(a)(ii) one from 1 A progesterone is low, at end / in E


progesterone concentration decreases ; A oestrogen concentration is low, at end / in E
oestrogen concentration decreases, near end / in D ;

6(a)(iii) corpus luteum ; 1

6(a)(iv) one from 1 A maintains the uterus lining


maintain the endometrium ; R maintains the, endometrium wall / uterus wall
negative feedback / inhibition, of, FSH / GnRH / LH ; I thickens lining
9700/41 Cambridge International AS/A Level – Mark Scheme May/June 2017
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Question Answer Marks

8(a) max 4 of: 4


1 different habitats ;

2 different niches ;

3 many (different) species / large variety of species ;

4 ref. to (much) genetic diversity within a species ;

5 different selection pressures ;

6 ref. to adaptation ;

7 different, climate / rainfall / temperature / soil / topography / conditions ;

8(b)(i) both sites are the same / no (significant) difference between two sites ; 1

8(b)(ii) genera 2 and species 4 ; 1

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Question Answer Marks

8(b)(iii) all figures to 3 d.p. to score but only penalise extra d.p. or rounding error associated with extra d.p. once 3

number on grassland
species n/N (n / N)2
not grazed
Onthophagus pennsylvanicus 6641 0.873 0.762
Canthon ebenus 774 0.102 0.010

Canthon pilularius 108 0.014 0.000


© UCLES 2017 Onthophagus hecate Page 13 of 18
85 0.011 0.000

total 7608 0.772

n / N figures correct / numbers of each species divided by total ;

(n / N)2 calculated and added up ; ecf from incorrect column 1 including figures with fewer / more than 3 d.p.

0.228 ; ecf total figure subtracted from 1

8(b)(iv) greater species evenness on grazed grassland ; ora A mostly, one species / O. pennsylvanicus, on not grazed 2

grazing increases (dung beetle species) (bio)diversity ; ora

if opposite conclusion reached check answer for (iii) and apply ecf for mp2 if D > 0.521

5 18.1.c, 18.2.a, 18.1.f J17/42/8

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Question Answer Marks

8(a) 1. random sampling ; max 4

2. (using) random number generator for coordinates ;

3. in both sites ;

4. measure, percentage cover / (Braun-Blanquet / ACFOR) scale cover ;

5. using (square frame) quadrats ;

6. repeat sampling ;

8(b)(i) family / sub-family ; 1

8(b)(ii) that there is no significant difference (between the two sites) ; 1

8(b)(iii) 3
animal taxon number n (n / N)2
present in N
soil under
brambles
pseudoscorpion 21 0.512 0.262
wireworm 12 0.293 0.086
gamasid mite 7 0.171 0.029
springtail 1 0.024 0.001
total 41 0.378

n / N figures correct or numbers of each species divided by total ;

(n / N)2 calculated and added up ;

total figure subtracted from 1 / 1 – 0.378 = 0.622 ; ecf

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© UCLES 2017 Page 14 of 19
Question Answer Marks

8(b)(iv) apply ecf from (iii) if D is very different to 0.663 / 0.622 2

1. bracken and bramble / both sites, have similar Simpson’s Index of Diversity (D) numbers ;
or
bracken and bramble / type of vegetation, has little effect on soil organism diversity ;

2. soil organisms more abundant under bracken ; ora

6 17.3.e, 18.3.c, 18.1.a N17/42/1


9700/42 Cambridge International AS/A Level – Mark Scheme October/November
PUBLISHED 2017
Question Answer Marks

1(a)(i) three from: 3

1 the numbers of, prey / herbivores / sheep / goats, would increase ;

2 decrease in number of plants / idea of overgrazing ;

3 damage to habitat ; e.g. erosion

4 increased competition (for plants) ;

5 lack of food causes, herbivore numbers to decline / herbivores to migrate away ;

6 reduction in, biodiversity / species diversity / genetic diversity / genetic variation ;

7 AVP ; e.g. increase in other predators / disruption to food web

1(a)(ii) one from: 1

1 snow leopards are, hard to see / camouflaged ;

2 vast habitat to cover (to locate snow leopards) / inaccessible locations / solitary ;

3 AVP ; e.g. difficulty getting permission from some governments to go into certain areas

9700/42 Cambridge International AS/A Level – Mark Scheme October/November


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Question Answer Marks

1(b) three from: 3

1 ban trade (of, leopard / leopard products) ;

2 ban hunting ;

© UCLES 2017 3 detection methods ; e.g. customs / trading officers / wildlife


Page 3 of forensics
20 / extra rangers

4 enforcement measures ; e.g. fines / prison / punishment

5 raise public awareness / education ;

6 ref. to protected areas / national parks ;

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Question Answer Marks

1(c) two from: 2

1 genetic diversity decreases / AW ;

2 increased chance of two harmful recessive alleles coming together / increase in inbreeding depression / decrease in
hybrid vigour ;

3 (some) less likely to survive ;

4 (because) less likely to adapt to change in, environmental conditions / selection pressure ;

1(d) two from: 2

1 appearance / morphology / visible features / looked similar to leopards ;

2 breeding (to produce fertile offspring) ; ora


© UCLES 2017 Page 4 of 20
3 ref. to behaviour ;

2(a)(i) NADP ; 1

2(a)(ii) two from: 2


7 18.1.b, 17.3.e, 18.2.c N17/43/1

9700/43 Cambridge International AS/A Level – Mark Scheme October/November


PUBLISHED 2017

Question Answer Marks

1(a) two from: 2

1 variation in / diversity of, ecosystems / habitats ;

2 number of / (how) many / variety of / diversity of, species ;

3 the (relative) abundance of each species ;

4 genetic diversity / range of alleles, within a species ;

1(b)(i) genes and environment ; 1

1(b)(ii) one from: 1

1 whales, mobile / swim / migrate ;

2 (they inhabit) large, area / distances ;

3 live, underwater / at great depths ;

1(c) two from: 2

1 (water) pollution from, industry / boats ;

2 accidents involving / damaged by, boats / fishing gear ;

3 lack of / competition for, food / krill / prey ;

4 noise / vibration, disturbs whale, communication / behaviour / mating ;

5 illegal, whaling / hunting ;

6 reproduction rate is slow / one offspring at a time / long gestation ;

© UCLES 2017 Page 3 of 19


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Question Answer Marks

1(d) two from: 2

toxins (PCBs / DDT) are


1 present in, water / river / sea ;

2 ingested / absorbed by, producers / phytoplankton / algae ;

3 bioaccumulation or toxins, pass up / accumulate up, food chain ;

4 persistent / long-lasting / not broken down (in environment / whale) ;

5 fat / lipid, soluble ;

1(e)(i) two from: 2

1 sodium ions do not enter (neurones / nerve cells / axons) ;

2 (neurones) cannot depolarise or cannot, generate / transmit, impulses / action potentials ;

3 reason for death ;

1(e)(ii) one from: 1

unicellular / not multicellular ;

motile / have flagella ;

8 18.1.e, 17.3.e, 18.3.c J18/41/1


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9700/41 Cambridge International AS/A Level – Mark Scheme May/June 2018
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Question Answer Marks

1(a)(i) any one from: 1


positive correlation ;
as area (of forest) increases, number / population / orangutans, increase(s) ; ora

1(a)(ii) 25 000 – 7300 2


2016–1985 ;
571 (per year) ;

1(a)(iii) 12.78 / 12.8 / 13 (years) ; 1

1(b) any two from: 2

1 hunting / poaching, for, bushmeat / (traditional) medicine / crop protection ;


2 hunting / (live) capture, for pets / trade ;
3 disease ;
4 breeding problems due to, individuals isolated / mates inaccessible / inbreeding in small populations ;

1(c) any three from: 3

1 breeding (programmes) ;
2 release / reintroduction (to, wild / Sumatra/ reserves) ;
3 educate / raise public awareness ;
4 research, diet / breeding / behaviour / genetic diversity ;
5 raise money for, reserves / protecting wild population ;
6 AVP ; e.g. cooperate with governments

9 17.3.e, 18.3.c, 18.3.d J18/42/1

9700/42 Cambridge International AS/A Level – Mark Scheme May/June 2018


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Question Answer Marks

1(a) any 3 of: 3


1 killed by humans ;

© UCLES 20182 difficulty in finding a mate ; Page 5 of 17

3 loss of habitat / habitat fragmentation / habitat polluted / deforestation ;

4 food shortage ;

5 (new) diseases ;

1(b) any 3 of: 3


1 maintains or increases, gene pool / genetic diversity / genetic variation ;

2 maintains or increases, heterozygosity ;

3 hybrid vigour ;

4 reduces / prevents, inbreeding depression ;

5 reduce chances of harmful recessive alleles coming together ;

1(c) any 2 of: 2


1 IVF or description;

2 embryo transfer ;

3 surrogacy or description ;

4 artificial insemination ;

10 17.3.e, 18.3.c, 18.3.d J18/43/1


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Question Answer Marks

1(a)(i) 0.03 ; 1

1(a)(ii) three from: 3

1 more humans result in fewer macaques / ora ;


2 (humans) kill macaques ;
3 (humans) destroy macaque habitat / named example ;
4 (humans) bring disease ;

1(b) four from: 4

1 captive breeding / assisted reproduction ;


2 release into wild ;
3 education ;
4 ban, hunting / trade ;
5 reserves / national parks / zoos ;
6 provide food ;
7 provide healthcare / monitor populations for disease ;
8 limit / ban, on new building ;

11 17.3.e, 18.3.c, 18.3.d, 18.2.a N18/42/1

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Question Answer Marks

1(a)(i) any two from 2


1 deforestation or habitat, destruction / fragmentation ;
2 killed ;
3 disease ;
4 reduction in food supply ;

1(a)(ii) any three from 3


1 captive breeding / AI / IVF / surrogacy ;
2 release into the wild / insurance populations ;
© UCLES 2018 3 education / awareness ; Page 5 of 17
4 (work with Philippine government to) set up reserves ;
5 research, diet / habitat / breeding / behaviour / genetic diversity ;
6 veterinary care / monitor health ;

1(b) any three from 3


1 hybrids formed / fewer warty pigs produced ;
2 may be sterile ;
3 genetically different (from warty pigs) ;
4 (hybrid) less adapted for natural environment ;
5 (hybrid) ref. to susceptibility to, diseases / parasites ;

1(c) Eukarya ; 2
R eukaryote(s) / Eukaryota
class ;

12 17.3.e, 18.3.c N18/43/1

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9700/43 Cambridge International AS/A Level – Mark Scheme October/November 2018
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Question Answer Marks

1(a)(i) any three from 3


1 habitat destruction ;
2 competition for food / food shortage ;
3 predation / predators ;
4 disease / infection ;
5 AVP ; e.g. pollution / pesticide use / removed from wild (to zoos) to conserve species / problems finding a mate

1(a)(ii) any two from 2


1 (captive) breeding ;
2 release into wild / insurance populations ;
3 education / increase awareness (of illegal bird trade) ;
4 research, diet / habitat / breeding / behaviour / genetic diversity ;
5 raise money / work with (Bali) government, to set up reserves ;
6 veterinary / health, care ;

1(b) any four from 4


1 low / little / less / decreased (genetic) (bio)diversity / variation ;
2 low / little / less / decreased, heterozygosity ;
3 harmful recessive alleles may come together ;
4 inbreeding depression ;
5 no / less / decreased, hybrid vigour ;
6 not / less, able to adapt to, new / changed, selection pressure / disease / environment ;

13 18.1.b, 18.1.f, 18.3.b J19/41/8

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Question Answer Marks

8(a) 1 C; 3
2 greater / highest / most, number of (plant + insect) species / species richness ;
3 greater / highest / most, number of habitats ;

8(b) area A because if one species, increases / decreases (in abundance), this may affect the whole, community / food web / 1
ecosystem ;
© UCLES 2018 Page 5 of 14
8(c) any one from: 1
1 species may have, medicinal / economic / aesthetic, benefits ;
2 (to maintain) genetic diversity / genetic variation / alleles ;
3 ref. to ethical / moral / stewardship ;

14 18.1.a J19/42/7
Question Answer Marks

9(a) any seven from: 7


1 reaction / photosynthesis, controlled / affected, by more than one / several, factors ;
2 factor / variable, nearest its minimum value / in shortest supply ;
3 which, prevents increase in rate / restricts rate / makes rate plateau ;

to increase crop yield in glass house:


4 light intensity + temperature + carbon dioxide concentration ;
5 artificial lights / lamps / provide lighting at night ;
6 heaters / open windows / control ventilation ;
7 CO2 from, cylinders / dry ice / combustion / (named) fuel heater ;
8 automation / computerised control / digital control ;
9 ref. to optimum, light intensity / temperature / CO2 concentration / conditions ;
10 AVP ; e.g. water / irrigation / sprinklers
control / optimum, humidity
(named) fertiliser / nitrate / manure

© UCLES 2019 Page 12 of 14


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9700/43 Cambridge International AS/A Level – Mark Scheme May/June 2019
Question PUBLISHED
Answer Marks
Question
7(a) any three from: Answer Marks 3
6(b)(ii) any three from: 3
1mitochondrial
functionalDNA
/ self-contained / specific, entity / unit / area ;
accept ora throughout

21 community
inherited from(ofmother
organisms)
alone ;/ all living organisms of all species / all populations of organisms ;

32 ref. to interactions
recombination ; over, doesn’t occur ;
/ crossing

43 abiotic / physical,
mutations, occur atand, biotic /rate
a constant biological, factors
/ molecular clock; A
; living and non-living

54 mtDNA mutates
idea that linked faster (than nuclear
by, energy DNA) ;cycling / food webs / food chains / nutrient cycle ;
flow / mineral

7(b) 5. many
niche ; copies of mtDNA (per cell) ; 3
6 is smaller
species ; / has fewer genes ;

7 not;associated with histones ;


abiotic
8 mtDNA analysis is quicker ;
15 18.1.d J19/43/7

Question Answer Marks

7 recapture ; 6

small / mobile / AW ;

harm ;

predators ;

mix / disperse / integrate ;

marked ;

16 18.1.c, 18.1.d, 18.1.f J20/41/8

© UCLES 2019 Page 14 of 20


© UCLES 2019 Page 16 of 21
9700/41 Cambridge International AS & A Level – Mark Scheme May/June 2020
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Question Answer Marks

8(a) any four from: 4

1 ref. to use of quadrats ;

2 ref. to size (of quadrat) ;

3 grid marked out ;

4 use of random number generator

5 measure species frequency ;

6 use of key or Braun Blanquet / other named, scale ;

7 measure, species abundance / percentage cover ;

8 repeat sampling ;

9 both sites treated the same ;

8(b)(i) 0.23 and 0.05 ; 1

8(b)(ii) Σ (n÷N)2 = 0.19 ; 2

D = 0.81 ;

8(b)(iii) field A has a higher species diversity than field B ; 1

17 18.2.a, 18.2.b, 18.2.c, 18.2.d J20/42/8

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Question Answer Marks

8(a) 2
5 1 8 6 2 4 3 7 ;;
© UCLES 2020 Page 16 of 20

all correct = 2 marks


four in correct consecutive sequence = 1 mark

8(b) 3
Fungi Plantae

method of nutrition heterotrophic autotrophic ;

storage polysaccharide glycogen starch ;

main component of cell wall chitin cellulose ;

8(c) bacteria ; 1

8(d) RNA or DNA ; 2

single-stranded or double-stranded ;

18 18.1.d, 18.1.e J20/43/8


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Question Answer Marks

8(a) any five from: 5

1 lay out a, line / tape ;

2 (line / tape) runs from low tide area to high tide area ;

3 ref. to use of quadrats ;

4 ref. to interrupted belt transect ;

5 place quadrats at regular intervals ;

6 measure species frequency ;

7 use of key or Braun Blanquet / other named, scale ;

8 repeat sampling ;

8(b)(i) B and C ; 1

8(b)(ii) Pearson’s linear correlation / Spearman’s Rank ; 1

19 18.1.d, 18.1.e N20/42/8

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Question Answer Marks

8(a) any five from: 5

1 mark–release–recapture ;

2 capture (sample of) rats and count them ;


© UCLES 2020 Page 16 of 20
3 ref. to humane traps ;

4 mark so as to not adversely affect rats


or
describe method of marking such as, tagging / using dyes ;

5 return to where they were caught / AW ;

6 allow time to mix (with population) / AW ;

7 capture second sample ;

8 count number caught and number marked ;

9 population size estimate (M1) =


number in first sample (N1 ) ×number in second sample (N2 )
;
number in second sample that are marked (M2 )

10 AVP ; e.g. repeat

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Question Answer Marks

8(b)(i) 6 × 164 2
rs = 1 –
83 − 8
or
984
=1–
504
or
= 1 – 1.95 ;

rs = – 0.95 ;

8(b)(ii) there is a strong correlation ; 2

negative correlation / as the altitude increases the mean height of the soft rush decreases ;

20 18.3.c, 18.3.f N20/43/3

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Question Answer Marks

3(a) any four from: 4


1 geographic(al), isolation / barrier between populations ;
2 no / low / less, (inter)breeding / gene flow ;
3 different mutations occur ;
4 different (named), selection pressures / environment(al conditions) ;
5 natural selection ;
6 genetic drift ;
7 allopatric (sub)speciation ;

3(b) cytochrome c oxidase / (mitochondrial) gene / DNA / base, sequences, 2


(nearly) identical / (very) similar ;
small number of bases different / low percentage difference ;

difference to other species is greater ;


© UCLES 2020 Page 19 of 23
3(c) any two from: 2
1 identify species, quickly / automatically / by non-experts ;
2 compare with, Red List / IUCN list / CITES appendix ;
3 confiscate / destroy / relocate, endangered species / ES products ;
4 prosecute / stop activities of, smugglers / traders / poachers ;
5 prevent entry of, predators / competitors / diseases, that could harm native ES ;

21 18.1.1, 18.1.2, 18.1.5, 18.1.4 J21/41/3

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Question Answer Marks

3(a)(i) any three from: 3


(individuals / organisms / members of a species are similar / same)
1 morphologically ;
2 physiologically ;
3 biochemically ;
4 genetically ;
5 ecologically / occupy same niche ;
6 behaviourally ;
7 can (inter)breed to form fertile offspring ;
8 reproductively isolated (from other species) ;
9 share, an evolutionary lineage / recent common ancestor ;
10 share a, binomial / Linnaean, name ;

3(a)(ii) domain: Eukarya ; 2


kingdom: Animalia ;

3(a)(iii) any two from: 2


1 classification / taxonomy, reflects / shows / depends on, evolutionary / phylogenetic, relationships ;
2 organisms / species, that, are closely related / share recent common ancestor, should be classified together ;
3 help identify, larger taxonomic groups / orders / families / genera ;
4 AVP ;

3(b)(i) (1478 × 103 × 2 =) 304 468 ; 1

3(b)(ii) any three from: 3


1 to, store / analyse / process ;
2 large quantity of data ;
3 to, compare / match, (many) sequences ;
4 to, calculate / measure / quantify, (percentage), similarity / difference ;
5 ref. to fast / accurate / efficient ;

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22 18.1.5, 18.3.6, 18.3.1 PUBLISHED J21/42/3
Question Answer Marks

3(a)(i) any three from: 3


© UCLES 2021 Page 8 of 16

1 multicellular ;

2 have, specialised cells / tissues / organs ;

3 heterotrophic / described ;

4 ref. to nervous system ;

5 vacuoles / vesicles, are, small / temporary / not permanent ;

6 are eukaryotes / have eukaryotic cells ;

7 detail of eukaryote ; e.g. nucleus / (named) membrane-bound organelle / 80s ribosomes

8 ref. to mobility ;

3(a)(ii) any three from: 3

1 records / assesses, biodiversity ;

2 so conservation decisions can be made / AW ;

3 example of conservation action 1 ;

4 example of conservation action 2 ;

e.g. (IUCN) red list of threatened species / identifying endangered species


(CITES) regulation of trade
ref. assisted reproduction or ref. captive breeding
seed banks
place in, zoos / national parks / marine parks / botanical gardens

5 (taxonomic hierarchy is) internationally, recognised / standardised / AW ;

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9700/42 Cambridge International AS & A Level – Mark Scheme May/June 2021
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Question Answer Marks

3(a)(iii) any two from: 2

1 (yes because as number of species has increased) biodiversity has increased ;

2 (idea of no because) numbers of new species that are being discovered are greater than the numbers of species
becoming extinct ;

3 (idea of no because) does not assess number of individuals in a species ;

4 AVP ; e.g. some species only exist in captivity

3(a)(iv) insects 2
1024 945
(×100 ) ;
1 438 805
71 ;

ecf – accept 1 mark if wrong data chosen

insects 1024 945


e.g. (×100 ) = 86
arthropods 1191770

arthropods 1191770
(×100 ) = 83
animals 1 438 805

3(b)(i) any two from: 2

1 habitat loss / habitat fragmentation / urbanisation / building / deforestation ;

2 insecticides / pesticides / farming / agriculture / fertilizers ; I pollution

3 new / increase in, predators / diseases / parasites ;


9700/42 Cambridge International AS & A Level – Mark Scheme May/June 2021
4 food shortage / increased competition (for food) ;
PUBLISHED
Question Answer Marks
© UCLES 2021 Page 9 of 22
3(b)(ii) any two from: 2

1 Bt toxin (only) kills, specific insects / insects that feed on the crops ;

2 reduces use of, insecticides / pesticides ;

3 idea that insects survive to pollinate plants ;

Question Answer Marks


23 18.1.1, 18.1.5, 18.3.5 J21/43/3
4(a) diabetes: 2
insulin from (GM) bacteria ; A ref to, yeast / animal cells

single gene disorder:


gene therapy
or
insert, functional / normal / dominant, allele into, target / somatic / stem, cells or genome ; A gene

R replacing one allele with another

© UCLES 2021 Page 10 of 22


9700/43 Cambridge International AS & A Level – Mark Scheme May/June 2021
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Question Answer Marks

3(a)(i) any two from: 2

1 differences in, morphology / phenotype / characteristics ;

2 differences in, physiology / biochemistry ;

3 genetic differences / AW ;

4 differences in behaviour ;

5 differences in ecological niche ;

6 isolated, populations / species


or
found in different geographical areas / AW
or
reproductively isolated / AW ;

3(a)(ii) any two from: 2

1 mate them together ;

2 see if offspring can interbreed / see if offspring are fertile;

3 ref. to bioinformatics investigation / described ;

3(b) any two from: 2

1 Fungi ;

2 Protoctist(a) ;
9700/43 Cambridge International AS & A Level – Mark Scheme May/June 2021
3 Prokaryota(e) / Prokaryote ; I bacteria
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Question Answer Marks

3(c) any four from: 4


© UCLES 2021 Page 8 of 22
1 hard to control / may become invasive / AW ;

2 may lack predators ;

3 may lack herbivores ;

4 may, prey on / parasitise, local / native, species / organisms ;

5 may compete with, local / native, species / organisms ; A description e.g. eat their food supply

6 may decrease, biodiversity / species richness / species abundance / AW ;

7 may have effects on, food web / ecosystem ;

8 may introduce diseases ;

3(d) any two from: 2

1 ban on African fruit should be lifted ;

2 can export more fruit / can export fruit to Asia / find new overseas markets ;

3 economic benefit / more work / more opportunities for farmers / social benefit ;

N21/41/3
24 18.2.3, 18.2.4, 18.2.6, 18.3.1, 18.3.3
N21/43/3

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Question Answer Marks

3(a) any four from: 4

1 use random sampling (technique) ;

2 repeat and find mean / multiply estimates up for whole area ;

3 (frame) quadrats to measure, plants / vegetation / stationary organisms ;

4 detail of using quadrat ;

5 mark-release-recapture for (named) animals ;

6 detail of, trapping / marking, (named) animals ;

7 calculate / use, Simpson’s index (of diversity) ;

8 species richness and, (relative) abundance / species evenness ;


9700/41 9 AVP ; Cambridge International AS & A Level – Mark Scheme October/November 2021
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Question Answer Marks

3(b) any four from: 4

1 climate change / end of (last) ice age / (global / local) warming ;

2 arctic fox, species / population, not well-adapted / unable to adapt ;

3 hunting, removed / caused extinction of, lynx / large predators ;

4 introduction of, named / alien / invasive / domestic, species / animal ;

5 may, eat / compete with / spread disease to, native, species / animal ;

6 overgrazing problem explained ;

7 may, change / destroy / disrupt, habitats / food webs ;


© UCLES 2021 Page 8 of 21
9700/41 8 AVP ; Cambridge International AS & A Level – Mark Scheme October/November 2021
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Question Answer Marks
9700/42 Cambridge International AS & A Level – Mark Scheme October/November 2021
3(c) any five from: PUBLISHED 5

Question 1 Answer
obtain, healthy / fertile / wild-caught / captive bred, (founder) animals ; Marks

4(c) any
2 three
ref. to from:
genetic, variation / diversity / testing ; 3

13 DNA analysis
(consider not available
if), sufficient for everyone
/ suitable, habitat / ;area, available ;

24 lifestyle
(consider change ; A e.g.prey
if) sufficient, lose/ food,
weight / stop smoking
available ; / take exercise

35 early
publictreatment ;
safety advice / education ;

46 allows people
(organise) to plan ; e.g.
compensation fororganise
farmers care
who /lose
appoint power; of attorney / take out medical insurance / make will / gift
livestock
property to children / retire early
7 outlaw, killing / disturbing, lynx ;
5 decide whether to have children / ref. (therapeutic) termination ;
© UCLES 2021
8 (plan to) monitor, lynx / other animal / prey, populations ;
Page 9 of 21
6 results may cause, anxiety / stress / panic / depression (if positive)
9 or
(plan to) cull / control / use contraceptives, if population, grows too much / spreads too far ;
reduce worry (if negative) ;
10 AVP ;
7 results may affect ability to get, insurance / credit / jobs ;

Question 8 idea of predictions may not be accurate ; Answer Marks


25 18.2.2, 18.2.4, 18.2.6, 18.2.5 N21/42/5
4(a)(i) 1 crime scene DNA may be present in, small amount / minute quantity ; 2
Question Answer Marks
2 PCR, amplifies / replicates / copies / multiplies, DNA ;
5(a) Fig. 5.1: species ; 3
4(a)(ii) 1 (negative) DNA moves to, positive electrode / anode ; 2
Fig. 5.2: genetic ;
2 smaller / lighter, fragments move, faster / further / longer distance ;
Fig. 5.3: habitat / ecosystem ;

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9700/42 Cambridge International AS & A Level – Mark Scheme October/November 2021
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Question Answer Marks

5(b)(i) plants: 4
1 quadrats ;

2 transects ;

3 ref. to use, ACFOR / Braun-Blanquet


or
estimate percentage cover (of species)
count species
calculate plus species abundance
record species density
AW species frequency ;

insects:
4 pooters / pitfall traps / nets / light traps / sugar traps ;

5 mark-release-recapture ;

accept in either section:

6 (identification) keys ;

5(b)(ii) estimate biodiversity: Simpson’s index (of biodiversity) ; 2

9700/42 test relationship: Pearson’s linearCambridge


correlation International AS & A Level
coefficient / Spearman’s – Mark
rank Scheme
correlation ; October/November 2021
PUBLISHED
Question Answer Marks

5(c) any three from: 3

negative
1 smaller crop area so lower yield ;

2 prairie strip insects may feed on crop ;

3 (so) less, profit / income ; must be linked to mp1 or mp2


© UCLES 2021 Page 15 of 24
positive
4 (soil) more fertile / described, so higher yield ;

5 less / no, fertiliser / phosphorus compounds / nitrogen compounds, used ; ignore minerals

6 (so) more, profit / income


or
reduction in expenses ; must be linked to mp4 or mp5

9700/41 Cambridge International AS & A Level – Mark Scheme May/June 2022


26 18.1.4 PUBLISHED J22/41/1(a)
Question Answer Marks

1(a)(i) Eukarya ; 2
Animalia ;

1(a)(ii) any one from: 1


warning to predators / to deter predators ;

AVP ;

1(b) any three from: 3


9700/41 Cambridge International AS & A Level – Mark Scheme May/June 2022
271 Ca2+ (stays) 18.3.1,
in, cytoplasm 18.3.2
/ sarcoplasm ; PUBLISHED J22/41/8
2 Ca2+, binds / bound, to troponin ;
Question 3 tropomyosin, shifted / does not cover (myosin) binding Answer sites (on actin) ; Marks
4 myosin-actin, cross bridges / bonds, exist / remain ;
8(a)(i)
© UCLES 2021 any
5 three from: / muscle, contracted / shortened / paralysed
sarcomere(s) Page 16;of 24 3
1 habitat loss ;
1(c) 2any for,
threeagriculture
from: / building / roads / forestry / mines / development ; 3
3 hunting / fishing ;
41 collecting
Ca2+, entersplants ;
/ diffuses into, synaptic knob ;
52 humans aid spread of (animal
through (voltage-gated) calcium / plant) disease ; ;
(ion) channels
63 humans
vesicles aid spread acetylcholine
containing of, alien / invasive
; / competing, species ;
74 (named)
move to /pollution
fuse with, / pesticides
presynaptic ; membrane ;
5 exocytosis of acetylcholine ;
8(a)(ii) 29 000  7000 29 000  7000 22 000 2
1 or ;
2000  1950 50 50

2 440 ;
9700/41 Cambridge International AS & A Level – Mark Scheme May/June 2022
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Question Answer Marks

8(b) any seven from: 7


1 (named) food ;
2 (named) medicines ;
3 wood / fibres / paper / rubber ;
4 genetic diversity for future use ;

5 science / technology / research ;


6 education ;
7 aesthetic / wellbeing ;
8 (eco)tourism / attract visitors ;

9 ethical / moral / stewardship ;


10 local cultural significance ;

11 maintain / protect / stability of, food chains / food webs ;

12 pollination / ecosystem services ;

13 protect against, soil erosion / coastal erosion / flooding ;


14 soil formation ;
15 (named) mineral, cycles / recycling OR ref. to stage in cycle of named element ;

16 climate stability ;

9700/42 Cambridge International AS & A Level – Mark Scheme May/June 2022


28 18.2.4, 18.3.1, 18.1.5 PUBLISHED J22/42/10
Question Answer Marks

10(a) any three from: 3

1 marking, not harmful (described) / cannot be removed (described) ;

2 constant population size ;

3 no, births / deaths (described)


© UCLES 2022
or Page 16 of 19
no, immigration / emigration / migration ;

4 (sufficient time for) marked individuals to mix with rest of population ;

5 (penguins are) mobile ;

10(b) any three from: 3

1 loss of habitat / example ;

2 climate change ;

3 predation ;

4 competition for, food / resources / breeding sites / example ;

5 new disease ;

6 pollution / example ;

7 numbers get so low that population can’t recover / AW ;


9700/42
10(c) 13 000 / 1.3  104 ; Cambridge International AS & A Level – Mark Scheme May/June 2022
1
PUBLISHED
Question Answer Marks

10(d) any four from: 4

1 multicellular ;
© UCLES 2022 Page 21 of 22

2 eukaryote / eukaryotic / (cells) contain a nucleus ;

3 specialised cells / tissues / organs ;

4 heterotrophic (nutrition) / described ;

5 nervous system ;

6 cilia / flagella ;

7 mobile / motile ;

29 18.3.1, 18.3.6, 18.3.3 J22/43/8


9700/43 Cambridge International AS & A Level – Mark Scheme May/June 2022
PUBLISHED
Question Answer Marks

8(a) any two from: 2

1 loss of habitat / described ;

2 climate change / global warming ;

3 predation ; I hunting

4 competition for, food / resources / breeding sites / example ; A not enough food

5 new disease ;

6 numbers get so low that population can’t recover / AW ;

8(b) any three from: 3

1 trade ban (if species is in danger of extinction) ;

2 if species is not (yet) at risk of extinction permit required ;

3 ref. to border controls / checks ; A fines / punishment if caught

4 (provide countries with) lists of species that are, rare / endangered ;

5 encourages governments, to join CITES / to abide by CITES regulations ;


9700/43 6 AVP ; Cambridge
e.g. every International
few years they have aAS & A Level with
conference – Mark
theirScheme
members May/June 2022
PUBLISHED
Question Answer Marks

8(c) any four from: 4

1 captive breeding / description ;

2 assisted reproduction / example ;

3 reintroduce into the wild ;


© UCLES 2022 Page 19 of 23

4 medical care ;

5 education / public awareness ;

6 research ;

7 projects in the field ;

8 ref. to maintaining genetic databases, to avoid inbreeding


or
working with other zoos ;

N22/41/1
30 18.3.1, 18.3.6, 18.3.4
N22/43/1

© UCLES 2022 Page 20 of 23


9700/41 Cambridge International AS & A Level – Mark Scheme October/November 2022
PUBLISHED
Question Answer Marks

1(a) any two from: 2

1 habitat loss ;

2 competition with / disease from, farm / invasive / alien, animals / species ;

3 climate change ;

4 hunting / poaching / killing by humans ;

5 AVP ;

1(b) any three from: 3

1 global / international / worldwide (authority / advisory) ;

2 (to give advice to) conserve / protect, nature / biodiversity / habitats ;

3 assess / categorise, species conservation status ;

4 ref. Red List (of Threatened Species) ;

5 influence, governments / policy ;

6 educate / raise awareness ;

9700/41 7 AVP ; Cambridge International AS & A Level – Mark Scheme October/November 2022
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Question Answer Marks

1(c) any four from: 4

1 mating / IVF / (super-ovulate female plus) AI ;

2 remove embryo (from, mated / artificially inseminated, female) ;

3 check / select, healthy / normal / best, embryos ;


© UCLES 2022 Page 5 of 19

4 may freeze embryos for, storage / later use ;

5 implant embryos in (different female) uterus ;

6 ref. surrogate (mother) ;

7 ref. (named female reproductive) hormone(s) ;

8 AVP ;

31 18.3.6, 18.3.4 N22/42/1

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9700/42 Cambridge International AS & A Level – Mark Scheme October/November 2022
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Question Answer Marks

1(a) any three from: 3

1 Red List ;

2 e.g. threatened / endangered / extinct etc., species ;

3 ref. education / spreads awareness ;

4 provides a global forum for sharing expertise / AW ;

5 provides advice about conservation issues ;

1(b) 1 cattle at edge / old and weak cattle, (easy) for lions to kill (for food) ; 2

2 (so) the remaining cattle can survive / AW ;

1(c) any four from: 4

1 female given, hormone / FSH / gonadotropins ;

2 ref. to superovulation / AW ;

3 (secondary) oocytes / ova, harvested / AW ; Ignore eggs

4 detail of harvest ; e.g. use of fine needle / using ultrasound

5 sperm / semen, added to (secondary) oocytes / ova ;

6 cultured / AW, for several days / until embryo or blastocyst stage ; Ignore number of cells

7 embryo / blastocyst, placed in uterus ;

SECTION B
32 18.2.b, 18.3.c J18/42/9
© UCLES 2022 Page 5 of 19

9700/42 Cambridge International AS/A Level – Mark Scheme May/June 2018


PUBLISHED
Question Answer Marks

9(a) any 8 of: 8


1 no, nucleus / nuclear envelope ;

2 circular DNA / plasmid ; A circular chromosome

3 cell membrane composition different (from, bacteria / eukarya) ;

4 no membrane-bound organelles / named example ;

5 70S ribosomes ;

6 cell wall present ;

7 cell wall does not contain peptidoglycans ;

8 cells divide by, binary / multiple, fission ;

9 (mostly) unicellular ;

10 (some) have histones associated with DNA ;

11 (some) are extremophiles / description ;

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9700/42 Cambridge International AS/A Level – Mark Scheme May/June 2018
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Question Answer Marks

9(b) any 7 of: 7


1 variety / collection, of seeds ;

2 long-term storage ;

3 (storage) to, prevent germination / maintain dormancy ;

4 detail of storage conditions ; e.g. frozen or stored at temperatures below –13 °C dried

5 seeds regularly tested for viability / description ;

6 seeds re-stocked regularly ;

7 maintain genetic diversity / genetic material preserved / acts as a gene bank ;

8 detail of future use ; e.g. can be used after environmental disaster / new diseases / used to increase numbers

9 maintains biodiversity ;

10 research / education ;

11 AVP ; e.g. takes up less space than growing plants / ref. to cheaper to store than whole plants

33 18.2.b, 18.3.d J18/43/9

9700/43 Cambridge International AS/A Level – Mark Scheme May/June 2018


PUBLISHED
Question Answer Marks

9(a) eight from: 8

1 nucleus ;
2 (double) membrane-bound organelles ; A example
3 80S ribosomes ;
© UCLES 2018 Page 18 of 20
4 chloroplasts / mitochondria, have 70S ribosomes ;
5 linear DNA ;
6 DNA associated with, histones / proteins ;
7 chloroplasts / mitochondria, DNA is circular ;
8 unicellular, colonial and multicellular ;
9 cells divide by mitosis ;
10 reproduction can be asexual and sexual ;
11 AVP ;

9(b) seven from: 7

1 useful for species where reproduction is difficult / AW ;


2 example ; e.g. from habitat fragmentation / reproductive physiology
3 increases the rate of, reproduction / offspring production ;
4 IVF ;
5 hormone treatment / superovulation ;
6 (many) oocytes harvested ;
7 (oocytes) mixed with sperm ;
8 embryos placed in uterus ;
9 surrogacy ;
10 detail ; e.g. use a similar species / non-rare breed
11 ref. to portmanteau animal / embryo transfer ;
12 AVP ; e.g. embryo splitting
cryopreservation of sperm
artificial insemination

34 18.2.b, 18.3.c N18/41/9

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9700/41 Cambridge International AS/A Level – Mark Scheme October/November 2018
PUBLISHED
Question Answer Marks

9(a) any eight from 8


1 no, nucleus / nuclear envelope ;
2 circular DNA ;
3 histone-like / HU / HN-S / INF, proteins associated with DNA ;
4 no membrane-bound organelles ;
5 named example of absent organelle ;
6 70S / 18 nm, ribosomes ;
7 cell wall made of, peptidoglycan / murein ;
8 binary fission ;
9 unicellular ;
10 spherical / rod / spiral / comma / corkscrew, shape or may form, pairs / tetrads / strings / filaments / clusters / palisades ;
11 size detail ;
12 Gram-negative have, extra / second / outer, membrane ;
13 AVP ; (other structural feature)
14 AVP ; (other non-structural feature)

9(b) any seven from 7


1 collect / take, plants / seeds, from the wild ;
2 (from) many countries / international effort ;
3 (especially from) areas at risk from climate change ;
4 grow / cultivate / plant, seeds / plants (in botanic gardens) ;
5 increase, plant / seed, number ;
6 tissue culture / cloning ;
7 (store in) seed bank ;
8 maintain / measure, genetic diversity or ref. to gene bank ;
9 cooperate with, governments / charities / agencies / universities ;
10 for research ;
11 to conserve habitats ;
12 to restore habitats ;
13 to reintroduce species (to wild) ;
14 educate (public) / raise awareness ;
15 raise funds ;
16 AVP ; e.g. prepare suitable habitats / growing conditions

35 18.2.c, 18.3.c N19/42/9


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9700/42 Cambridge International AS/A Level – Mark Scheme October/November 2019
PUBLISHED
Question Answer Marks

9(a) any seven from: 7

Fungi and Animalia


1 eukaryotic cells ;

2 & 3 details of eukaryotic cells ;; e.g. nucleus / linear DNA / chromosomes / histones / 80s ribosomes /
(named) membrane-bound organelles

4 heterotrophic / described ;

5 ref. to glycogen ;

Fungi only
6 some unicellular ;

7 hyphae / mycelium ;

8 multinucleate parts ;

9 ref. to spores ;

10 cell walls of chitin ;

Animalia only
11 multicellular ;

12 specialised cells ;

13 differentiated into, tissues / organs ;

14 some motile ;

15 (some cells have) cilia / flagella ;

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9700/42 Cambridge International AS/A Level – Mark Scheme October/November 2019
PUBLISHED
Question Answer Marks

9(b) any eight from: 8

methods
1 provide as natural environment as possible / described ;

2 storage of, sperm / eggs / gametes ; A sperm banks

3 artificial insemination / IVF ;

4 embryo transfer / surrogate mothers ;

5 can monitor, health of mother / development of foetus ;

6 (international) cooperation between zoos ;

7 genetic records kept / ‘stud’ book ;

8 release into the wild ;

problems
9700/43 9 may be stress in captivity ; Cambridge International AS/A Level – Mark Scheme October/November 2019
PUBLISHED
10 mate may be rejected ;
Question Answer Marks

8 111 glycolysis
reproductive
; cycles may be disrupted (in captivity) ; 6
2 cristae ;
312 &ATP
13 named problems
synth(et)ase ; with release ;; e.g. difficulty in finding food / may not integrate into groups / more susceptible to
4 protonsdisease / very little
/ hydrogen ions natural
/ H+ ; habitat left to release animals into
5 matrix ;
366 Krebs / TCA18.2.d, 18.3.g
/ citric acid, cycle ; N19/43/9

Question Answer Marks

9(a) any eight from: 8


1 not cellular ;
2 contain, nucleic acid / genetic material / DNA / RNA (core) ;
3 DNA may be single-stranded or double-stranded ;
© UCLES 2019 Page 20 of 22
4 (core surrounded by) protein coat / capsid / capsomeres ;
5 may have, external / lipoprotein, envelope / membrane ;
6 20–750 nm ;
7 obligate parasites ;
8 reproduced / replicated, in / by, host cells ;
9 disease-causing / pathogenic ;
10 no, metabolism / respiration / nutrition / excretion / growth ;
11 cannot move / immobile ;
12 have, proteins / enzymes, to help, infection / replication ;
13 (highly) specific to host (cells) ;
14 not (thought to be) living ;
15 AVP ; e.g. lytic / lysogenic, life cycles
antigenic, variability / drift / shift

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9700/43 Cambridge International AS/A Level – Mark Scheme October/November 2019
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Question Answer Marks

9(b) any seven from: 7


1 name 1 ;
2 name 2 ; e.g. WWF / Greenpeace / Nature Conservancy / Wildlife Conservation Society / Oceana / Sea
Shepherd / Conservation International / CITES / IUCN / IFAW / WAZA / World Seed Bank / IPBES
3 raise, funds / donations ;
4 influence, governments / businesses ;
5 ban / reduce, hunting / polluting / oil drilling / mining / deforestation ;
6 research / reports ;
7 conserve, species / populations / habitats / biodiversity OR prevent extinction ;
8 education / publicity campaigns / raise awareness ;
9 hold protests OR take direct action to prevent, development / exploitation ;
10 promote coexistence of wildlife and people ;
11 regulate / legislate for, trade in wild species ;
12 estimate / monitor / categorise, threatened / endangered, species ;
13 detail of CITES trade categories ;

Question Answer Marks

10(a) any seven from: 7


1 action potential / depolarisation / impulse, at pre-synaptic membrane ;
2 (voltage-gated) calcium ion channels open / calcium ions enter (cell / cytoplasm / (motor) neurone / pre-synaptic knob) ;
3 vesicles fuse with pre-synaptic membrane ;
4 acetylcholine / ACh, released, by exocytosis / into synaptic cleft ;
5 (ACh) binds to receptors on, muscle cell membrane / sarcolemma / motor end plate ;
6 sodium ion channels open / sodium ions enter (muscle cell / sarcoplasm) ;
7 depolarisation of, (muscle) cell surface membrane / sarcolemma ;
8 (depolarisation) spreads / transmitted, to / down / via, T-tubules ;
9 depolarisation of (adjacent) sarcoplasmic reticulum (membrane) ;
10 (voltage-gated) calcium ion channels open ;
11 calcium ions, move / diffuse, out of SR / out of cisterna(e) ;
12 calcium ions, move / diffuse, into, sarcoplasm / cytoplasm ;
13 calcium ions, start contraction / bind to troponin ;

© UCLES 2019 Page 13 of 14

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