Experiment No 1

Good Laboratory practices and safety measures

Chemicals Required

Glass ware Quantity

Equipment
Experiment No 2

Study of Microscope

Chemicals Required

Glassware Quantity

Equipment

Microscope 4
Experiment No 3

Preparation of Nutrient agar

Chemical requirement:

1. Powdered nutrient agar (50g)

2. Distilled water (Quantity sufficient)

3. Cotton (I pack)

Glassware Quantity

1. Conical flask 10

2. Aluminum foil 1 pack

3. Sterilized Petri plates 10

Equipment:

1. Autoclave 1

2. Laminar flow hood 1

3. Weighing balance 2
Experiment No 4

Preparation of Nutrient broth

Chemical requirement:

1. Nutrient broth powder (50g)

2. Distilled water (Quantity sufficient)

Glassware Quantity

Conical flask (100ml) 10

Beakers 10

Glass Tubes 10

Falcon tubes 10

Stirrer 10

Equipment:

Autoclave 1

Laminar flow hood 1

Weighing balance 2
Experiment No 5

Enumeration of microorganism

Chemical requirement:

1. Nutrient agar powder (50g)

2. Tap/ pond water sample (10ml)

Glassware Quantity

Sterile petri plates 10

Pipette 5

Marking pen 7

Pipette bulb 7

Mechanical pump 7

Equipment:

Water bath 4

Autoclave 1

Laminar flow hood 1

Colony counter 5

Incubator 1
Experiment No 6

Catalase test

Chemical requirement:

1. Bacterial colony

2. H2O2 (20ml)

Glassware: Quantity

Glass slides 10

Wooden applicator sticks 10

Test tube 10

Equipment:

Laminar flow hood 1

Spirit lamp 5
Experiment No 7

Coagulase test

Chemical requirement:

1. Bacterial sample

2. Distilled water (Quantity sufficient)

3. Blood Plasma

Glassware: Quantity

Glass slides 10

Wax pencil 5

Test tubes 10

Holder 10

Spirit lamp 5

Equipment:

Laminar flow hood 1

Spirit lamp 5

Incubator 1
Experiment No 8

Identification of bacteria by Gram staining

Chemical requirement:

1. Methanol (10ml)

2. Crystal violet dye (10ml)

3. Distilled water (Quantity sufficient)

4. Bacterial colony

5. Safranin (10ml)

6. Iodine (10ml)

Glassware: Quantity

Glass slides 10

Bunsen burner 5

Equipment:

Microscope 4

Laminar flow hood 1

Slide Stand 2
Experiment No 9

Microbiology of air

Chemical requirement:

1. Nutrient agar (50g)

Glassware: Quantity

Flasks 100ml 10

Petri plates 10

Beakers 100ml 10

Inoculating loop 5

Equipment:

Autoclave 1

PH meter 3

Laminar flow hood 1

Incubator 1
Experiment No 10

Microbiology of Water

Chemical requirement:

1. Agar (50g)

2. Water sample

3. Distilled water (Quantity sufficient)

Glassware Quantity

Flasks 100ml 10

Beakers 100ml 10

Petri plates 10

Inoculating loop 5

Equipment:

Autoclave 1

Laminar flow hood 1

Weighing balance 4

Incubator 1
Experiment No 11

Fungal Staining

Chemical requirements

1. Lactic acid (70ml)

2. Phenol (70ml)

3. Glycerol (140ml)

4. Cotton blue/Aniline blue (0.2g)

5. Distilled water (Quantity sufficient)

6. Sabouraud dextrose agar (SDA) (50g)

Glassware Quantity

Dropper 5

Inoculating loop 5

Glass slides 10

Cover slips 10

Beakers 100ml 10

Pipette 5

Graduated cylinder 100ml 10

Equipment:

Autoclave 1

Laminar flow hood 1

Compound light microscope 7

Incubator 1
Experiment No 12

Microscopic test for malaria

Chemical requirement:

1. Cedar wood oil (20ml)

2. Romanowsky stains (10ml)

3. Buffered distilled water (Quantity sufficient)

4. Distilled water (Quantity sufficient)

Glassware Quantity

Glass slides 10

Applicator sticks 10

Cover slips 10

Disposable needles 10

Gloves 14

Pipette 5

Equipment:

Laminar flow hood 1

Compound light microscope 7