Scribd Logo
Upload

Welcome to Scribd!

  • Tarea 22

    Uploaded by

    Andres ML
    1 views4 pages
    1) The document presents data from a gel filtration chromatography experiment separating various proteins and molecules by molecular weight. 2) It lists the molecular weights, elution volumes, and elution volume/void volume ratios for several proteins and compounds that were run through the column including urease (590 kDa), glutamine (350 kDa), catalase (125 kDa), carbonic anhydrase (13 kDa), and citric acid (5 kDa). 3) A graph is shown plotting the elution volume/void volume ratio against the log of the molecular weight, from which the molecular weights of two unknown protein samples (estimated at 60 kDa and 200 kDa) can be determined

    Original Description:

    Original Title

    TAREA 22(1)

    Copyright

    © © All Rights Reserved

    Available Formats

    XLSX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    1) The document presents data from a gel filtration chromatography experiment separating various proteins and molecules by molecular weight. 2) It lists the molecular weights, elution volumes, and elution volume/void volume ratios for several proteins and compounds that were run through the column including urease (590 kDa), glutamine (350 kDa), catalase (125 kDa), carbonic anhydrase (13 kDa), and citric acid (5 kDa). 3) A graph is shown plotting the elution volume/void volume ratio against the log of the molecular weight, from which the molecular weights of two unknown protein samples (estimated at 60 kDa and 200 kDa) can be determined

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    1 views4 pages

    Tarea 22

    Uploaded by

    Andres ML
    1) The document presents data from a gel filtration chromatography experiment separating various proteins and molecules by molecular weight. 2) It lists the molecular weights, elution volumes, and elution volume/void volume ratios for several proteins and compounds that were run through the column including urease (590 kDa), glutamine (350 kDa), catalase (125 kDa), carbonic anhydrase (13 kDa), and citric acid (5 kDa). 3) A graph is shown plotting the elution volume/void volume ratio against the log of the molecular weight, from which the molecular weights of two unknown protein samples (estimated at 60 kDa and 200 kDa) can be determined

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 4

    BIOGEL CP-5

    kDa mL 140
    125
    UREASA 590 48
    120 110
    GLUTAMINA 350 60
    CATALASA 125 76 100
    ANHIDRASA 13 110 80

    Axis Title
    CITRCROMOC 5 125
    ? -- 90 60
    ?? -- 70 40

    20

    Vol. Total = 135 0


    1 10
    PESO MOLECULAR kD
    48 0.35555556
    60 0.44444444
    76 0.56296296
    110 0.81481481
    125 0.92592593
    90 0.66666667
    70 0.51851852

    kDa Vo/Ve 3
    Ureasa 590 0.3555556
    Glutamina S 350 0.4444444
    Catalasa 125 0.562963
    Anhidrasa Carbonica 13 0.8148148 2.5
    Cit. C 5 0.9259259
    P1 -- 0.6666667
    P2 -- 0.5185185
    2
    PM
    Proteína 1 60 kDa
    Preoteina 2 200 kDa
    1.5
    Ve/Vo

    1 5; Column D;
    0.9259259259259264; Colum
    0.81481481

    0.5

    0
    5; Column D;
    0.9259259259259264; Colum
    0.81481481

    0.5

    0
    1 10

    PM kDa
    BIOGEL CP-5
    125
    110

    76
    60
    48

    10 100 1000
    PESO MOLECULAR kDA

    5; Column D;
    0.9259259259259264; Column D;
    0.814814814814815
    3; Column D;
    0.5629629629629632; Column D;
    0.444444444444444
    1; Column D;
    0.355555555555556
    5; Column D;
    0.9259259259259264; Column D;
    0.814814814814815
    3; Column D;
    0.5629629629629632; Column D;
    0.444444444444444
    1; Column D;
    0.355555555555556

    10 100 1000

    PM kDa

    You might also like