IV.

Results and Discussion

1. Results
Table 1:

Concentratio
0 10 20 30 40 50 60 X Y
n
0.28 0.414 0.63 0.866 0.934 0.918 0.939 0.721 0.959
Absorbance
8 5 6 6 5 6 7 2 8
0.126 0.34 0.578 0.646 0.630 0.651 0.433 0.671
ΔOD 0
5 8 6 5 6 7 2 8

0.7
f(x) = 0.0134148351648352 x
R² = 0.956430894298507
0.6

0.5

0.4

0.3

0.2

0.1

0
2 0 10 20 30 40 50 60 70

Graph 1: Standard absorbance curve

2. Discussion
Rarely, if ever, do empirical research or statistical software report R-squared indices
of goodness of fit for count data. For the fundamental Poisson regression model and
for more general models, such negative binomial that consider overdispersal data, we
present a number of R-squared measures based on different definitions of residuals.
[1]
The higher the value R2, the more accurate the trend line. In scientific, the R2 must be
above 0.95 to be considered as a reliable statistic.
In the graph above, the R2 value is 0.9564, which is just slightly higher than 0.95. The
R2 value in this experiment could be higher, up to over 0.98. It can be some mistakes
in preparing the BSA solution, which leads to the wrong concentration of the solution.
In addition, mistakes could be found in the process of adding Bradford reagent into
the 96-well plate. Bubbling or not exact volume of reagent is one of the common
mistakes in this experiment.
[1] Cameron, A. C., & Windmeijer, F. A. (1996). R-squared measures for count data
regression models with applications to health-care utilization. Journal of Business &
Economic Statistics, 14(2), 209-220.