How to use Colilert*

Presence/Absence Test Procedure

1
Carefully separate one snap pack from the strip, taking care not to accidentally open adjacent pack.

5
Read the results at 24 hours. Compare each result against the comparator dispensed into an identical vessel.

Open one pack by snapping back the top at the scoreline as shown. Caution: Do not touch the opening of pack. Add the reagent to a 100 mL water sample in a sterile, transparent, nonfluorescent vessel.

3
Aseptically cap and seal the vessel and then shake until dissolved.

If no yellow color is observed, the sample is negative for total coliforms and E. coli. If the sample has a yellow color equal to or greater than the comparator, the presence of total coliforms is confirmed. If color is not uniform, mix by inversion, then recheck. If the sample is yellow, but lighter than the comparator, it may be incubated an additional 4 hours (but no more than 28 hours total). If the sample is coliform positive, the color will intensify. If it does not intensify, the sample is negative. If yellow is observed, check vessel for fluorescence by placing a 6 watt 365 nm UV light within five inches of the sample in a dark environment. Be sure the light is facing away from your eyes and towards the vessel. If fluorescence is greater or equal to the fluorescence of the comparator, the presence of E. coli is confirmed.

these test reactions should be seen in Colilert and Colilert-18:

4
Incubate for 24 hours at 35 0.5C.
COLORLESS Negative for total coliforms and E. coli. YELLOw Positive for total coliforms. FLuORESCEnt* Positive for E coli.
*Use 365nm UV lamp

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Please refer to product insert for further information. *Colilert is a trademark or registered trademark of IDEXX Laboratories, Inc. in the United States and/or other countries. The IDEXX Privacy Policy is available at idexx.com.

How to use Quanti-Tray* and Quanti-Tray*/2000

Quantification method for Colilert*, Coliert*-18, Colisure* and Enterolert*

1
Open snap pack and add reagent to 100 mL of water sample in a sterile vessel.

4
Incubate according to each IDEXX reagents product insert.

Squeeze the upper part of the QuantiTray so that it bends towards the palm. Open the Quanti-Tray by pulling the foil tab away from the well side. Pour the reagent/ sample mixture directly into the Quanti-Tray. Avoid contact with the inside of the Quanti-Tray.

Read each result by comparing it against the QuantiTray Comparator. Count positive wells and refer to the MPN table. Refer to each product insert for specific reading instructions.

Place the samplefilled Quanti-Tray onto the rubber tray carrier of the Quanti-Tray Sealer with the well side (plastic) of the Quanti-Tray facing down to fit into the carrier. Seal according to the Quanti-Tray Sealer instructions.

For more information visit idexx.com/water or call 1-800-321-0207

2011 IDEXX Laboratories, Inc. All rights reserved. 09-71166-02 *Colisure, Enterolert and Quanti-Tray are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries. The IDEXX Privacy Policy is available at idexx.com.