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  • DSP Expt 1

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    reallydestiel
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    Experiment 1 sought to disrupt bacterial cell walls enzymatically using lysozyme. Lysozyme was added to cultures of E. coli and M. luteus to degrade the cell walls. Absorbance readings before and after lysozyme addition showed that the E. coli cell wall was disrupted, decreasing in absorbance, while the M. luteus cell wall remained intact. This confirms that lysozyme is able to enzymatically disrupt bacterial cell walls through degradation of peptidoglycan.

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    Experiment 1 sought to disrupt bacterial cell walls enzymatically using lysozyme. Lysozyme was added to cultures of E. coli and M. luteus to degrade the cell walls. Absorbance readings before and after lysozyme addition showed that the E. coli cell wall was disrupted, decreasing in absorbance, while the M. luteus cell wall remained intact. This confirms that lysozyme is able to enzymatically disrupt bacterial cell walls through degradation of peptidoglycan.

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    © All Rights Reserved
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    9 views4 pages

    DSP Expt 1

    Uploaded by

    reallydestiel
    Experiment 1 sought to disrupt bacterial cell walls enzymatically using lysozyme. Lysozyme was added to cultures of E. coli and M. luteus to degrade the cell walls. Absorbance readings before and after lysozyme addition showed that the E. coli cell wall was disrupted, decreasing in absorbance, while the M. luteus cell wall remained intact. This confirms that lysozyme is able to enzymatically disrupt bacterial cell walls through degradation of peptidoglycan.

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    © All Rights Reserved
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    of 4

    EXPERIMENT 1.

    cEL DiSRUP TION BY NZYMTIC METHO>.

    To disuwpf ullwall uius


    JMio coccu Omd

    E.celiby ysoyme

    Hiyly
    vamte bac
    oualopev
    ins:EDTA
    das tallie titon X.
    o GvaAm -e

    Caslesgen ipopolSacchosidda

    pptidogy Cow accundee : s

    tna i hens.
    a l wall Aramiay

    teud brokuw y ysozye


    K-1-4-gyosicie
    RMymanc
    teinul
    tcd pew

    Guwh agilahsm prefap ast


    mey malhe mefud.
    PAe Coaminnsy M

    s a o awoailabe besics Ruso2ym


    yco ilass

    NAM- NAG Sokaga bvek yAroayn


    fw Gra t e amo-e
    7soye wevks

    PROCEPURE
    l pherthale b
    O-1M phopha bol (5+45md diailed 4,o).

    Mi ero co cemo Reuties amod E.co ltun

    C Berep kl 5 0-TH Ahahali t t


    Pup ysoy m swotim amdand ld tal

    CO-25 m osamdard ysozyme+ 6.75 phosphase huPeu)


    Bloh 0-1M phosphale
    butte, (callbrale 0.00)

    DD 460 m
    oyioayme
    waif 60 nmconcs.
    Adr 100jk
    &tiv tov 15 mc .

    0.7 op be mamtoina d. (CAls acwe, nenundi


    05 0Dt
    plnaine
    add phesphale btta
    OD
    7 0-7
    hece 0D, H &ozqne oD
    kve ulls absevb w.

    A t o dismpluw D value dacaa


    belwuw 0-5 0 7 OD Vuu.
    Take ading By
    Specb phsio mafe.
    dt. HD, 0-00 a 7 Twspame
    Coabrafe JiR

    Blomk, 450 «w, adius phoak lotfa (blank


    000 a absowbamce agge.
    s insivuueud
    450
    450wm (w3 ow

    Blam 0D aud to 0.00 e. coli.


    450m
    Taka tne E. uoli JAA
    tle
    hak OD a

    vidug OD 0-602

    wnel ullue
    hadkhen 100p o tmice
    Miospipalt yso
    dadd to uh. ix wel
    Kp aide kov I miw.

    wwwie, take OD

    iduo o.561
    obrewaly;
    00 dacuaasd, ncicadug l l disA Bptin

    nas ccned

    call ditunum
    ivmatry T Ad msclia
    coloniks Lo

    Rotins
    S o m t

    os >07 odlled pephota bubbo


    Op w os
    d D.5- 0-7 OD vadaut

    0BSER VATION TABLE

    Cultu
    attyso
    addi usvm

    Lamk O.0O
    O0

    E. C
    O 602 o 56

    M. Leutuw O. 6 4 o.29

    T lwall o Mic euews amol E. celi


    eSOLT
    dimupded wmg

    DisCUSSION:
    wall waundy Cown ya
    alrd cal d ww Cwi
    Omd a cls

    w.bvae
    Gulnioe Mama
    wwwww k
    cll nwall cewmish
    o doat uio kow
    aud Gamd
    N awyl munac
    dhains
    iatamel
    a u l auuss amin
    by i / tJnas feptle haus
    i disuupled all
    was
    dkmupy
    wthod
    tedmias RDwaui
    uthod. mpd

    pd ramw
    Lyey
    amd NLgodan actin OD
    mAanad
    5 0 mw wwekugt.

    0-5 0-T OD Amaman ha


    atune amd Rpomial pnas

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