4200028B.

STS
SERVICE DATA BULLETIN
LAS Technical Support Specialist Group
710 Bridgeport Avenue, MS255
Shelton, CT 06484-4794

DISTRIBUTION: AA INTERNAL DIST. ONLY: ⌧ YES NO

PREPARED BY: Sarah Howden April 21, 2009 PAGE 1 OF 50 PAGES

AA 6/7/800 Optical Alignment & Test Procedure

PROBLEM:

PURPOSE:

Optical alignment and test procedures for the AAnalyst 600/700/800

spectrometers.

MATERIAL REQUIRED:

GENERAL:

Optical alignment uses the 16 bit base modules.

1
 Optical Alignment AAnalyst 600/700/800
Index

1 REQUIRED TOOLS 5

2 BEFORE USING THE MERCURY LAMP 8

2.1 REMOVE THE INSTRUMENT COVERS 8

2.2 REMOVE DETECTOR BOARD 9

2.3 CONFIGURE THE SE4200 BASE MODULE (BM) 9

(not needed with 32 bit software)

2.4 REMOVE BEAM SPLITTER 10

2.5 MONOCHROMATOR ADJUSTMENT 10

2.5.1 SLIT REFERENCE ALIGNMENT 10

2.6 ZERO ORDER ALIGNMENTS OF THE WAVELENGTH DRIVE. 13

2.6.1 MECHANICAL AND ELECTRICAL ZERO ORDER. 13

2.7 INSTALL THE MERCURY LAMP 16

2.7.1 HEIGHT OF PARABOLA P1 16

2.7.2 SIDE OF PARABOLA P1 17

2.8 GRATING ADJUSTMENT 18

2.8.1 HEIGHT ADJUSTMENT OF THE GRATING 19

2.8.2 TILT OF GRATING 20

2.8.3 OPTICAL ZERO OF GRATING 20

2.9 PARABOLA FOCUS ADJUSTMENT 20

2.10 OFFSET WAVELENGTH ADJUSTMENT 24

2
3 SAMPLE BEAM ADJUSTMENT 25

3.1 FLAT MIRROR E4 ADJUSTMENT 25

3.2 TORROID MIRROR T2 ADJUSTMENT 26

3.3 FLAT MIRROR E2 ADJUSTMENT 27

3.4 TURRET ADJUSTMENT 28

3.4.1 REF. POSITION ADJUSTMENTS 28

3.4.2 HEIGHT ADJUSTMENT OF T1 30

3.4.3 SIDE ADJUSTMENT OF T1 31

3.4.4 HEIGHT ADJUSTMENT OF E1 31

3.4.5 SIDE ADJUSTMENT OF E1 32

3.4.6 FOCUS ADJUSTMENT OF T1 32

4 TURRET CALIBRATION 33

5 D2 AND REFERENCE BEAM ADJUSTMENT 34

5.1 BEAM SPLITTER HEIGHT AND SIDE ADJUSTMENTS 35

5.2 HEIGHT, SIDE AND FOCUS ADJUSTMENT OF T3 MIRROR. 35

5.3 PREPARATIONS FOR FINAL REF. BEAM ADJUSTMENT. 36

5.4 HEIGHT AND SIDE ADJUSTMENT OF E3 MIRROR. 37

5.5 D2 LAMP ADJUSTMENT 37

6 DETECTOR ADJUSTMENT 38

6.1 D2 ADJUSTMENT 40

7 WAVELENGTH CALIBRATION 41

3
7.1 CONDITIONS FOR THE WAVELENGTH CALIBRATION 41

7.2 STARTING THE WAVELENGTH CALIBRATION 41

8 FINAL TEST 45

8.1 ENERGY CHECK 45

8.2 MONOCHROMATOR RESOLUTION 46

8.3 COUNT COMPARISON TEST AA700/800. 47

8.4 SPECIFICATION TEST 48

9 RECONFIGURE THE SE4200 BM (not needed with 32 bit software)

10 OPTICAL PATH DRAWING 49

10.1 Form for recording motor steps & other parameters 50

4
1 Required Tools: B312-0322 Optical Alignment kit
Parts of the kit:

B312-0345 Tool Case

B312-0325 Mercury Lamp Assembly
B312-0346 Lamp Power Supply (220/230V)
B312-0331 Lens

(Lens – is attached to the mercury lamp assembly)

B312-0323 Excentric Adjustment Tool

(has a green handle, used to align the detector)

B312-0334 Furnace Compartment Target

(It is placed across the sample compartment)

B312-0338 Standard Linear Mirror Cover (Flat metal cover)

5
B312-0340 Standard Lamp Compartment (looks like a lamp, has a target)
(Place the target closer to the front of the instrument, needs to be the same
depth as the cathode in the lamp is. )

B312-0342 Standard Swiveling Mirror Fixture, (this is placed in the holes that
are used for the cover support). The image on this fixture has to be looked at
from the back of the instrument.

B014-3386 Optical Square (It has a mirrored surface)

B015-6118 Target Fixture

(has a line at a height of 70 mm, used with the 70 mm cross hair fixture)

B015-6119 70 mm Cross Hair Fixture

(Placed in the monochromator so a 70 mm line image appears on the Target
Fixture)

B015-7062 Parabola Adjustment Screw (circular tool placed on the large

parabola screw to enable a fine adjustment)

B015-2698 Spindle Oil 10mL

B085-7815 Socket Wrench 5.5 mm

B085-8791 Universal Joint 1/4"

6
B085-0115 Grip 1/4"

B085-3060 L-Shaped Hexagon Spanner 2,0 mm

B085-9713 Hexagon Spanner 5x320 mm

B085-6368 Torx-Screwdriver T20x200 mm

B085-6955 Torx-Screwdriver T20x90 mm

B085-6583 Torx-Screwdriver T10x80 mm

B085-1395 Feeler Gauge

TM
Additionally you need the following single element Lumina
HCLs:
For Wavelength Calibration
1 N305-0121 Cu Copper HCL
1 N305-0108 Ba Barium HCL
1 N305-0139 K Potassium HCL

For Analytical Specification Test:

1 N305-0119 Cr Chromium HCL
For Monochromator Resolution
1 N305-0152 Ni Nickel HCL

In the SE4200 base module, the icons from left to right are:
1. Save
2. Print
3. Preview
4. Calibrate Instrument (picture of instrument)
5. Align Lamps (lamp with cable)
6. Slit Parameter (rainbow colors)
7. Lamp Parameter (light bulb)
8. Gain Functions (display with energy bar)
9. Wave Drive (blue waves)
10. Read (stop light)
11. Peak Results (peak shape)
12. Continuous Display (blue display with reading)
13. Stop (stop sign)
14. Optic Alignment (wrench)
15. Direct Command
16. Instrument Info (Letter “I”)

7
2 Before You Start
To complete the optical alignment procedure described below, the atomizer must
be in the uninstall position for the AAnalyst 700/800 (use the ACU base
module to do this), the THGA must be swung out in the AAnalyst 600 (there
are two latch positions) and the instrument must be powered on. In addition, take
out all of the HCL/EDL lamps. The optical alignment must be performed in a dark
area.
The base modules are located in: C:\Program Files\WinLab32-
AA\Diagnostics\AAnalyst 600 or 700 or 800 (choose the instrument you are
using). To close a base module, click in the upper left hand corner of the window.

2.1 Remove the Instrument Covers

. • Power off the instrument. Unplug or turn off the circuit breaker.
.
. • Take off the AS-800.
.
. • Completely remove the top tilt-back cover. First remove the two
side screws, so it can be lifted up first.
.
• Remove the top-right side cover (two small screws on each side on
top). Do not remove all of the screws in between!

. • Disconnect the lamp compartment piston and remove the cover

(two screws). Do not lose the spacer that enables the piston to
move freely!
.
. • Remove the exhaust tube for the D2 lamp from the back wall of
the burner compartment.
.
. • Remove all internal optic covers. (held on by lock down clamps)
.
. • Remove the black cover of the lamp holder. This cover is difficult
to put back, due to all of the cables that are under it.
.
. • Remove the burner compartment assembly including the burner
door (two screws on the left and right hand sides - two screws in
the back). This leaves nothing surrounding the sample
compartment. Keep the two sections together!
.
. • Remove the baffle on the right side of the sample compartment.
This has a square cut out. Mark the position on the optical base
before removing and note the orientation. This baffle must be
replaced before the back light is turned off, so the back light
can be centered through it.

8
Warning! Risk of 450 Volts DC shock on the HCL lamp connectors
when the instrument is powered on!

2.2 Remove Detector Board

. • Remove the two clamps from the ferrite plate (mounted with a clip
to the spectrometer controller board front cover).
.
. • Unplug the flexible ribbon detector cable connector from the
Instrument Controller board. Be careful with the pins!

. • Remove the four screws shown in the figure above to remove the
detector (use the short Torx driver found in the alignment kit).
.
. • Take off the detector plate with detector board. Be careful; make
sure the quartz window of the detector is not damaged. Place Kim
Wipes or other similar material around it and store in a safe place.

2.3 Configure the SE4200 Base Module (BM)

. • Power on the instrument without any lamp installed.
.
• Access the SE4200 base module: C:\Program Files\WinLab32-
AA\Diagnostics\AAnalyst 600 or 700 or 800 (choose the instrument
you are using)

Note: For AAnalyst 700/800 it is necessary to run the SE4200 BM in

double beam (Flame) mode. The default is furnace mode. If the
base module is started again, flame mode must be selected
again.

9
 • To change the operation mode from single to double beam mode while
the instrument is initialized, go to the TOOLS pull down menu and
open the OPERATION MODE window and change to Flame.

2.4 Remove the Beam Splitter

. • Remove the fixed beam splitter including the bracket plate from the
optical base (AAnalyst 700).
.
. • Remove the swiveling beam splitter (AAnalyst 800) from the light
path by using the command in the Lamp Parameter window. The
command is at the very bottom of the window. If the base module
is started again, the beam splitter will have to be moved out
again.

2.5 Monochromator Adjustment

2.5.1 Slit Reference Alignment

• Open the Slit Parameter window by clicking on the rainbow in the
tool bar of the SE4200 BM. (Rainbow icon, 6th one from left hand
side.)

• Select the 2.0 nm Low slit position.

10
• Place target fixture (B015-6118) in front of the slit assembly.

• Move the slit motor with single steps until the 70 mm height line on the
target fixture matches the 70 mm height mark on the slit assembly.
There is a “hatch” line on the slit assembly at the 70 mm position. Look at
the fixture at a low position near the optical bench to get accurate optical
height.

Note: Clicking on the right arrow moves the slit down toward the optical
base.

Take a close look at the figure below! Notice that there are two rectangles for
each slit size (sample & reference). The position of the sample and reference
rectangles will reverse between the exit and entrance slits. The exit and
entrance slits are also not at the same height, but the sample portion for
the entrance & exit slits are centered on the 70 mm line.

11
 View from Parabola to Slit Assy.

• Press the Save button to store the value into the FLASH RAM on the
Lamp Controller Board. The number previously saved is printed on the
label that is underneath the instrument cover. (See the last page)

Note: There is no need to adjust the Hall sensor board to set the reference
position of the slit, just write down the number of steps for the
reference position. You will need this number if a new Lamp
Controller Board is installed.

• Go back and forth between several slit positions to confirm

proper slit alignment. The 70mm height line on the target fixture
must match the 70mm mark on the slit assembly.

Note: If the above test fails it may be that the slit gear is pushed up against
the slit housing too tightly causing it to bind and miss steps. If this
occurs, loosen the set screws on the stepper motor slit drive and
reposition the motor to reduce the contact pressure and provide easier
movement.

12
 • Repeat going back and forth between slit positions to reconfirm
proper operation.

2.6 Zero Order Alignments of the Wavelength drive.

2.6.1 Mechanical and electrical zero order.
The mechanical and electrical zero order of the wavelength drive is performed in
one step.
The grating arm must be parallel to sine bar! A gap of approximately 0.5mm
occurs between the arm and the sine bar as a result of the ball at the end of the
shaft. This gap must be uniform (parallel) over the entire length of the
mechanism. Make sure the ball is still connected to the end of the shaft!

• Open the Wavedrive window by clicking on the blue waves in the

tool bar of the SE4200 BM. (The “waves” icon, 9 over from left)

13
• In the Wavedrive window (left side), press Find Ref. Pos.

After pressing Find Ref. Pos., the wavelength drive moves back until the Hall
sensor enters the magnetic field and stops. In that position, the grating arm
must be parallel to the sine bar.

• Place the feeler gauge between the sine bar and grating arm. The
blade should just fit through the gap if aligned correctly and have the
same fit over the entire length of the bar.

14
 If the grating arm looks like the diagram below:

Grating Arm

• Loosen the Torx screw that attaches the Hall sensor, and move the
sensor closer to the stepper motor.

If the grating arm looks like the diagram below:

• Loosen the Torx screw of the Hall sensor and move the Hall sensor
closer to the back of the spectrometer.

• If the grating arm looks like the figure below, and the gap is 0.5 mm, it
does not need to be adjusted.

. • Tighten the setscrew of the Hall sensor (if it was loosened).

.
. • If an adjustment was made, test by pressing Find Ref. Pos.
again.

15
2.7 Install the Mercury Lamp
Remove the fiber optic from the slit assembly. (It is put back after
focus is finished.)
If the fiber optic is in place, the top section of the slit will appear as if the light is
not going through the slit. (Be careful not to damage the tip of the fiber optic.)

. • Remove the fiber optic from the slit assembly board. Loosely put
Kim Wipes around the fiber optic. Use a T9 Torx.
.
. • Install the lens onto the mercury lamp assembly.
.
. • Mount the mercury lamp on the side of the instrument frame in front
of the exit slit.
.
. • Plug in the power cord of the mercury lamp into the output socket of
the mercury lamp power supply (B001-4672).
.
. • Connect the mercury lamp supply power cord to the power outlet
on the rear of the instrument. Do not use the COOLING SYSTEM
outlet because this is software controlled.
.
. • Align the position of the mercury lamp to produce a bright, uniform
light on the parabola surface. Use a piece of white paper (i.e.
business card) in front of the parabola P1 (it is not necessary to
remove the parabola mask, but it must stay level or the image will
be distorted). Make sure the light is bright, even, and passes
through only one slit, otherwise the light will not be seen in the
sample path. It is very important to get a bright image.

2.7.1 Height of Parabola P1

. • Select the 2.0 nm Low slit position in the Slit Parameter window.
.
. • Place the optical square (mirror) (B014-3386) between the
parabola P1 and the grating, facing the parabola and directing the
reflected beam onto the entrance sample slit (slit on right side).
.
. • Using a 3mm L-shape Allen wrench adjust the height of the
parabola with the top and bottom screw on the holder of the
parabola to ensure full overlapping of the light image on the
entrance slit. To assure the proper adjustment is made always use
both screws going back and forth. On the exit slit (left slit), the
sample slit is on the top. On the entrance slit (right slit), the

16
 sample slit is on the bottom. Center the sample slits to the 70
mm line. See picture on the next page.
.
. • Remove the optical square (mirror).

2.7.2 Side of Parabola P1

. • If installing a new parabola, make sure the mask is on the parabola
before proceeding.
.
. • Open the Wavedrive window by clicking on the blue waves in the
tool bar of the SE 4200 BM.

17
Using the wavelength entry (left side of window), of the Wavedrive window,
type in 40 nm and press the Set button.

. • Continue stepping the wavelength drive in small increments to get

the smaller, left-hand image just touching the left-side edge of
the grating mask (looking at the grating from directly in front of it -
not from behind it). With the light in this position, the right-side
edge of the larger image must fall off the right side of the grating
mask by 5mm. (Verify the above using a card with a 5mm line
drawn on it.)
.
. • If the above adjustment is not correct make a side-to-side
adjustment on P1 using the left and right adjustment screws. This
procedure increases/decreases the distance between the two
images.
.
. • Repeat the above two steps to achieve the proper alignment as
shown in the drawing below.
.
. • Recheck the height adjustment of the parabola P1.
.

5 mm

2.8 Grating Adjustment

The height and tilt of the grating must be aligned to ensure that both the white
and green light are at exactly the same height as the entrance slit. Use the
2.0nm Low slit position.

18
2.8.1 Height Adjustment of the Grating

. • Using the wavelength entry field of the Wavedrive window, drive the
wavelength drive to approximately 0 nm. Change the wavelength
drive position by typing in different wavelengths (close to 0 nm) or
by manually moving the wavelength drive (best access point:
damping wheel on the shaft of the stepper motor) so that the light
image is half overlapping the slit.
.
. • Use a 2 mm Allen key. With white light (0 nm) adjust the height of
the grating such that the light image has the same height as the
entrance sample slit. Match the 70 mm line to the middle of the
sample portion.
.
. • If the image is too low, carefully turn the height screw counter-
clockwise.
.
. • If the image is too high, carefully turn the height screw clockwise.

19
2.8.2 Tilt of Grating
. • Find the wavelength reference point by clicking on the Find Ref.
Pos. button in the Wavedrive window.
.
. • Use the wavelength entry field in the Wavedrive window to drive the
wavelength to 546.1nm. Change the wavelength drive position by
typing in different wavelengths (± 546.1nm) or by manually moving
the wavelength drive (best access point: damping wheel on the
shaft of the stepper motor), so that the green light image is half
overlapping the entrance sample slit.
.
. • With green light (546.1nm) adjust the tilt of the grating, so that the
light image has the same height as the entrance slit
.
. • If the image is too low, carefully turn Tilt screw counter-clockwise
.
. • If the image is too high, carefully turn Tilt screw clockwise.

Caution: Be careful when removing the screw driver, so the

grating arm ball does not bounce onto the sine bar!

• If a large tilt adjustment was required in the previous step, than the
white light image may need to be readjusted for height.

2.8.3 Optical Zero of Grating (at 0 ‫ג‬, light is centered in slit)

. • In the Wavedrive Window press Find Ref. Pos.
.
. • Carefully adjust the optical zero screw, on the right hand side of the
grating, so white light (0 wavelength) is centered (side to side) in
the entrance of the sample slit.
.
. • Verify step 2.8.1 and 2.8.2, (height and tilt of grating).

2.9 Parabola Focus Adjustment

The parabola mirror has to be adjusted for rotation and focus so that the focus of
the beam is exactly at the slit disk. This alignment is very important because the
resolution of the optical system is directly related to the precision of the focus
alignment! (Proper focus adjustment is verified by a scan with a nickel lamp.)
When exchanging the parabola, check the parabola rotation in its mount. There
is a “hash” mark on the parabola that should be 70 mm above the optical base.
This mark must be on the side of the sample compartment, not to the back of the
instrument.

20
Note: The parabola is not symmetrical to its axis, a sharp rectangular image at
the entrance slit can be obtained only if the hash mark appears on the side
of the furnace compartment.)
To monitor the focus point, it is necessary to have the E4 flat optic mounted at
the entrance slit.

The focus of the Parabola can be adjusted with either white or green light using
the 0.2 nm Low slit! DO NOT USE A 2.0 SLIT, IT MUST BE 0.2 NM!
. • Carefully cover the mercury lamp to prevent stray light from
entering the slit area. Also, make sure the heat from the hot
mercury lamp remains adequately ventilated. Try using aluminum
foil to prevent stray light.
.
. • Using the wavelength entry of the Wavedrive window, drive the
wavelength drive to 546.1 nm (green light).
.
. • Place a white card between E4 and T2. (Or where the baffle was
removed from, to the right of the sample compartment).
.
. • Slightly loosen the two set screws locking the parabola in position.
.
. • By moving the wavelength drive manually clockwise and counter-
clockwise (use the damping wheel), the light image can be moved
over the entrance slit. This creates a light image on the white card
at the baffle.
. • One of the following 4 images will appear on the white card:

21
.

1. Focus and rotation maladjusted:

A more or less narrow light image is moving from the lower left corner
to the upper right corner (or vice versa) or from the lower right corner
to the upper left corner (or vice versa).

2. Focus maladjusted:
A more or less narrow light image is moving from the left side to the right
side (or vice versa).

22
3. Rotation maladjusted:
A more or less narrow light image is moving from the top to the bottom (or
vice versa).

4. Correctly adjusted:

Over the entire area, the light appears smoothly, reaching a maximum intensity
and then disappearing. The image appears to collapse on itself, but there is no
movement from left to right, up and down, or diagonally.

. • By carefully rotating the parabola, and using the parabola

adjustment key for a forward and backward adjustment, optimize
the parabola rotation and focus. Perform the rotation alignment first.

23
 Turn the large back center screw slowly to correct side to side
movement, this moves the mirror in and out. Rotate the mirror
to correct up and down movement, but keep the “hash” mark
close to its 70 mm position.

. • At the end of the alignment tighten the parabola locking screws.

Make sure the epoxy or copper inserts are underneath the set
screws to prevent damage to the parabola shaft. These
adjustments may result in a slight height change and it may be
necessary to readjust the parabola height at this time.

At this point, make sure the height, side-to-side and zero order adjustments
of P1 are still OK.

• Important: Reinstall the fiber optic! This makes

sure, that the sample beam is guided through
only. Block the input to the fiber optic with a
thick piece of paper to eliminate dual images.

2.10 Offset Wavelength Adjustment

There is no need to calibrate the wavelength accuracy at this time because it’s
done during the wavelength calibration procedure.

24
3 Sample Beam Adjustment

The sample beam of the spectrometer optics will be aligned in the following
steps. White light, with the 2.0 nm L slit at the Ref. Position of the wavedrive
assembly, must pass through the entrance slit.

• Take out the baffle at the right hand side of the burner compartment. (This
was previously done with the monochromator adjustments.)

3.1 Flat Mirror E4 Adjustment (small mirror to right of

the slits)
. • Place a white card or piece of paper in front of Torroid T2.
.
. • Perform the horizontal (side) adjustment of E4 (by turning the
adjustment screw) to center the light image onto Torroid T2 (this
is not the height adjustment - no fixture is needed).

25
. • Now make the height adjustment (screw next to the top arrow) on
E4 using the cross-hair and 70mm target fixtures.
.
. • Place the 70 mm cross-hair fixture (B015-6119) in the
monochromator between the wavelength drive assembly and the
parabola. Adjust the cross hair fixture position to get a sharp
image of the hair line on the target fixture at T2. Try to use the
same position for the cross-hair fixture for height adjustments of
other optics.
.
. • Adjust the height screw on E4 to superimpose the cross-hair image
onto the lined fixture in front of T2.
.
. • Remove the 70 mm cross hair fixture that is in the monochromator.
.
. • Remove the target fixture from the front of Torroid T2.

3.2 Torroid Mirror T2 Adjustment (right side of sample

compartment)

. • Place the furnace compartment target (B312-0334) in the furnace

compartment.
.
. • Looking down at the optical bench from in front of the instrument,
you will see a small pin below the flat optic E2 (left side of the
sample compartment). Align the L-shape side of the fixture
directly above the center of this pin. (A newer System has a hole
instead of a pin.)
.
. • With the fixture in the above position, the light image should appear
square and focused. Slide the fixture back-and-forth in the
compartment to confirm the focal point.
.

26
. • If the focus is not correct (image is not clear), than loosen the
screws holding Torroid T2 (right side of sample compartment) to
the optical bench and move the optic forward and backward.
.
. • If the shape is not square, than the rotation is incorrect and the
rotation screws need to be adjusted.
.
. • After alignment is completed tighten the lock down screws.
.
. • Remove the furnace compartment target.

. • Now, using a 2 mm Allen key, adjust the side-to-side movement

of T2 to get the light image centered on the flat mirror E2 (left
side of sample compartment).

. • Place target fixture (B015-6118) in front of flat mirror E2

.
. • Place 70 mm cross hair fixture (B015-6119) in the monochromator
in the center of the wavelength drive assembly and position it to get
a sharp image on the target fixture at E2.
.
. • Using a 2 mm Allen key adjust the height of T2 to superimpose
the image of the cross-hair onto the target fixture.
.
. • Remove the target fixture from in front of the optic.

3.3 Flat Mirror E2 Adjustment (Left side of sample

compartment)

27
The position of the E2 mirror is fixed by two set pins.

. • Place the target fixture (B015-6118) in front of Torroid mirror T1,

the tilting mirror (for lamp alignment). Make sure the fixture rests
on the polished part of optical bench.
.
. • Using a 2 mm Allen key adjust the height of E2 to match the 70 mm
line of the target fixture and the 70 mm line image of the cross-hair
fixture.
.
. • Remove the target fixture and the 70 mm cross hair fixture.
.
. • Adjust side-to-side of E2 (2 mm Allen screw) to center the light
image on Torroid T1. Use a piece of paper the size of the optic for
centering. If T1 is set to a height of 70 mm, the side to side
adjustment is a little easier. Setting the Vertical position to 135
(Pos. Vert. 135, then Set A), makes the mirror straighter rather than
tipped forward.

3.4 Turret Adjustment

Since there is a fixed 8 position lamp housing used in the Analyst 600/700/800,
the linear mirror E1 (moves from side to side), and the tilting mirror T1 is used to
select the up/down lamp positions. The linear mirror is positioned in front of the
selected HCL/EDL lamp position, and the T1 tilting mirror chooses the upper or
lower lamp.

3.4.1 Ref. Position Adjustments

• Open the Lamp Parameter window by clicking on the light bulb in the
tool bar of the SE4200 BM.

28
 • In the Turret-Adjust section of Lamp Parameter, press Ref. Pos.

The stepper motor for the linear mirror E1 moves to the left side Hall sensor
position.

. • Move the Hall sensor position until you get about 1 - 2 mm

distance between the endblock and the black casting on the
linear drive screw. (To the left of the magnet.)
.
. • Press Ref. Pos several times to verify the correct Hall sensor
position.

The stepper motor for the swivel mirror T1 moves to the Hall sensor position. In
this Ref. position the distance between the movable casting and the optic
mount should be 0.5 mm. Use a feeler gauge to check for proper spacing.

29
 • Reposition the Hall sensor to change this distance. Press the
Ref.Pos. button after each Hall sensor adjustment.

These adjustments ensure that the linear mirror (E1), and the Torroid mirror
(T1), stop their movement by the Hall sensor before they reach a physical
stop.

3.4.2 Height Adjustment of T1 (Tilting Torroid)

. • Type 135 in the entry field for Pos. ver. and press the Set A. Pos.
button. This positions T1 to an approximate height of 70mm.
.
. • Place the target fixture (B015-6118) in front of E1 (linear mirror).
.
. • Place the cross-hair fixture in the monochromator and move it until
you see a sharp image of the height line projected on the target
fixture.
.
. • From the Base Module press the Up/Down buttons until the height
line from the jig matches the height line of the target fixture.
.
. • Remove the jigs and write down the number of steps for the
vertical position. (Pos. ver.)
# of steps __________

30
3.4.3 Side Adjustment of T1 (Tilting Torroid)

• In the Lamp Parameter Window type in the Pos. hor entry field 8000
and press the Set A. Pos button.

This will allow adequate room to place the standard linear mirror (B312-0338)
(flat metal target fixture) on the surface of E1.

. • Loosen the two screws for the Side and the Main Fixing Screw
(shown in the picture on page 33, Fixing Screws for Focus and Side
of T1 Mirror Assy.). Center the light image of the reflected beam on
the mask by means of moving the complete T1 mirror assembly.
.
. • Tighten the Side screws and the Main Fixing Screw.

. • If necessary, rotate the T1 mirror by using the 3 fixing screws

(indicated in picture on page 30, showing the 0.5 mm at Ref. Pos.),
until you get a symmetrical image on the optical mask.
.
. • Remove the optical mask. from the surface of E1.

3.4.4 Height Adjustment of E1 (Linear Mirror)

• Remove the metal support bracket for the top Cover.

In the Lamp Parameter Window type in the Pos. hor. entry field 10,000 and the
previously determined steps for the Pos. Vert., and press the Set A. Pos
button. This causes the E1 mirror to move to the casting and touch it and
provides enough room to make the following height adjustment.

. • Place the target fixture (B015-6118) on the pads with the two
holes on top of the optical base in front of the E1 mirror (where the
support bracket was removed from).
.
. • Place the cross-hair fixture in the monochromator and move it until
you see a sharp image of the height line.
.
. • Turn the height screw of the E1 (linear) mirror until the line from the
height jig matches the marked line of the target fixture.
.
. • Remove the cross-hair fixture from the monochromator.
.
. • Remove the target fixture B015-6118.

31
3.4.5 Side Adjustment of E1 (Linear Mirror)
In the Lamp Parameter Window type in the Pos. hor entry field 10,000 and the
previously determined steps for the Pos. Vert. and press the Set A. Pos
button. This causes the E1 mirror to move to the casting and provides enough
room to make the following side-to-side adjustment.

. • Put in the standard swiveling mirror (B312-0342) in the two holes

on top of the optical base in front of the E1 mirror (where the
support for the cover was). Look at the target from the back of
the instrument.
.
. • Turn the side screw of the E1 mirror until the image of light is
centered between the two marked vertical lines of the jig.
.
. • Remove the standard swiveling mirror from in front of the E1
mirror.

3.4.6 Focus Adjustment of T1

. • Insert the lamp alignment fixture (B312-0340) into the first lamp
position. The internal target must be nearer the front of the instrument and
the nearer edge of the fixture should be flush with the front edge of the
lamp compartment housing (do not push the fixture all the way in until it
hits the stop). The target should be at the same depth as the lamp cathode.
.
. • In the Lamp Parameter Window click on Ref. Pos.
.
. • Enter 300 in the Pos. hor. Field and 50 for the Pos. ver. Field.
.
. • Confirm the value by pressing the Set A. Pos. button. The light

32
 image should be visible near the center of the lamp fixture.
.
. • Manually enter step numbers for vertical and horizontal to
center the light image on the lamp fixture.
.
. • Loosen the Main Fixing Screw and the screws for Focus that
hold the mirror to the optical bench.
.

Fixing Screws for Focus and Side of T1 Mirror Assy.

. • Move the complete T1 mirror assembly forward and backward to

get the sharpest image possible on the lamp fixture.
.
. • Tighten the Focus screws.
.
In the Lamp Parameter window press the Ref. Pos. button.

4 Turret Calibration
Since there is no longer a rotating turret, the swivel and linear mirrors must
move to optimize the light throughput for each lamp position. The combination of
step values must be obtained separately for each position using the following
calibration procedure and those values are then stored into the flash prom on the
lamp controller board.

The approximate number of steps for the horizontal (stepper motor for
mirror E1) and vertical (linear stepper motor for mirror T1) are given in the
table below:

33
 Lamp 1 2 3 4 5 6 7 8

Pos. hor. 300 300 3200 3200 6040 6040 8900 8900

Pos. ver. 80 310 80 310 80 310 80 310

Note: A minimum numbers of steps for the pos. vert. of 50 is required

to allow proper turret adjustment range.
1. Insert the standard lamp compartment (B312-0340) fixture into
lamp position one. The target should be closer to the front.
2. In the Lamp Parameter Window click on Ref. Pos.
3. In the field for Pos. hor. and Pos. ver. enter the values shown
above for lamp position 1.

4. Now click the Set A. Pos. button to activate the values.

5. With the Up/Down & Left/Right buttons center the light image onto
the center hole of the lamp fixture.

5. In the Lamp Parameter Window enter 1 in the Number entry field.

7. Store the values found for the first lamp position by pressing the
Set L. Pos. button.

8. Record the number of steps found for each individual lamp

position on paper. These values will be needed in case the lamp
controller board is replaced. Write down these numbers on the
last page of this procedure.

9. Remove the lamp fixture from position one and place in position
two. Repeat steps three to eight for all lamp positions.

Press the Save button in the Turret- Adjust field to store all of the values into
the flash prom of the lamp controller board.

5 D2 and Reference Beam Adjustment

Note: There is no Ref. Beam and D2 Adjustment for the AA600.
The mercury lamp and 2.0nm L slit are still required for the following
adjustments.

34
5.1 Beam Splitter Height and Side Adjustments

. • Reinstall the fixed beam splitter (AA700) or reposition the

movable beam splitter (AA800) back into the light path by using
the command located in the Lamp Parameter window of the base
module.
.
. • Remove the T3 mirror mount (mirror that directs the D2 light) and
put the 70 mm height fixture in place of the T3 mirror.
.
. • Put the cross-hair fixture back into the monochromator.
.
. • Turn the height screw of the beam splitter until the height line from
the jig matches the height line of the target fixture. A bright light
from the mercury lamp is needed for this adjustment.
.
. • Reinstall the T3 mirror and move the mirror mount, to get side to
side, and front to back, focus pre-alignment inside the D2 lamp
compartment. Use a card in front of the D2 compartment.
.
. • Adjust the side-to-side movement of the beam splitter for the
AA700 by turning the complete assembly so that the image of the
reflected light is symmetrical with respect to T3.
.
. • Adjust the side-to-side movement of the swiveling beam splitter
for the AA800 by turning the eccentric end block until the image of
the reflected light is symmetrical with respect to T3. If the range of
the eccentric end block is too small, move the complete beam
splitter base for rough adjustment.
.
. • Verify the height adjustment of the beam splitter.
.
. • Do not remove the cross-hair target, it is needed for the next step.

5.2 Height, Side and Focus Adjustment of T3 Mirror.

. • Place the height jig in front of the D2 Lamp on top of the two
pads with the holes. (Make sure the pads are flat!)
.
. • Turn the height screw of the T3 mirror until the height line from
the crosshair fixture matches the height line of the target fixture.
.
. • Remove the cross hair fixture.
Turn the side screw of the T3 mirror until the light image is

35
 centered in the entrance window of the D2 lamp assembly.

5.3 Preparations for Final Ref. Beam Adjustment.

. • Exit the SE4200 BM.
.
. • Before turning off the mercury lamp, reinstall the baffle (to the
right of the sample compartment) and center to the light beam.
.
. • Remove all jigs from the optical base.
.
. • Switch off power to the mercury lamp and allow the housing to cool
down.
.
. • Take out the mercury lamp and remove the lens.

• Turn off instrument power (plug or circuit breaker).

.
. • Reinstall the detector board (four screws). Use the short T20 Torx
driver.
.
. • Re-attach the ferrite device onto the instrument controller board
housing and plug in the cable connection to the 4200 Controller
Board.
.
. • Reinstall the monochromator cover.
.
. • Put a Cu HCL into lamp position one.
.
. • Make sure that the D2 lamp is installed and connected.
.
. • Switch power back on to the spectrometer.
.
. • Activate the AA800 Base Module.
.
. • Make sure that the flame mode is selected in the Operation
Mode Window, or no reference counts will be seen!
.
. • Open the Lamp Parameter window and click on the Ref. Pos.
button.
.
. • Open the Slit Parameter window and click on the calibrate
button. You will have to choose the 2.0nm LOW slit to reset the slit
back to this position.
.
. • Select lamp number 1 and click on the Set button to activate.

36
.
. • In the lamp current entry field type in 15 mA for Cu and click on the
Set button.
.
. • Select Beam Splitter In (AAnalyst 800).

5.4 Height and Side Adjustment of E3 mirror.

. • Loosen the E3 (reference beam) optic and move it until the image
of the beam splitter is centered on it.
.
. • Align the height and side-to-side adjustments of E3 so the light
image is centered on the entrance to the fiber optic.

5.5 D2 Lamp Adjustment

. • Open the Lamp Parameter Window and enter 100mA for the D2
lamp current and click the Set button.
.
. • Adjust height, side, and focus of the D2 lamp with the appropriate
adjustment screws so that the focal point of the D2 image is in line
with the focal point of the HCL image as seen in the sample
compartment & the entrance of the fiber optic. Look both places.

• Turn off the D2 lamp.

Note: The final D2 lamp adjustments will be performed using the detector
and Read window.

37
6 Detector Adjustment
The detector adjustment is performed with the primary wavelength from the Cu
HCL using the Sample Beam.

. • Install the Cu HCL into the first lamp position.

.
. • In the Lamp Parameter Window select the lamp number 1 and
confirm with the Set button.
.
. • In the Lamp Parameter Window enter 15 mA in the Lamp Current
field and click the Set button.
.
. • Open the Slit Parameter Window and select the 0.7nm L slit.
.
. • In the Wavedrive Window press the button Find Ref. Pos.
.
. • In the Wavedrive Window enter 324.8nm in the Wavelength
entry field and confirm the entries by pressing the Set button.
.
. • Open the Test pull down menu and disable the ref. beam by
removing the check mark. (This makes sure, the turret is peaking
from the sample beam.)
.
. • In the Wavedrive Window press the Find Max button.
.
. • In the Lamp Parameter Window press the Find Max button.
.
. • Open the Read Window and select:
Signal linAA

38
 Read AA-BG

. • In the Read Window press the Start button.

The numbers of counts for the Sample Beam are displayed.

• Open the fixing screw for the horizontal adjustment with the
short Torx screwdriver, put in the excentric tool into the point of
horizontal adjustment and carefully move the detector plate to
get the maximum number of counts for the sample beam.

Fixing Screws for

Vertical Adjustment

. • Tighten the fixing screw for the horizontal adjustment.

.
. • In the Read Window press the Stop button.
.
. • Open the Test pull down menu and enable the Ref. Beam. Do not
press the find max button in the Lamp Parameter window.

Note: The maximum for the Ref. Beam will now be adjusted with the 0.7
nm H slit position.

. • Open the slit parameter window and select the 0.7 nm H slit
position.
.
. • In the Read Window press the Start button.

The numbers of counts for the Reference and Sample Beam are displayed.
(If there are only Sample counts, make sure Flame is selected in Operation
Mode.)
. • Carefully adjust the side-to-side and height of the flat mirror E3 to

39
 get maximum counts for the reference beam (reference counts).
.
. • Block the sample beam.
.
. • Open the fixing screw for the vertical adjustment , put in the
excentric tool into the point of vertical adjustment and carefully
move the detector plate with the excentric tool to zero the counts
for the sample beam (analytical counts).

Note: A maximum of 60 counts is admissible for the sample beam with

the sample beam blocked.

. • Tighten the fixing screws for the vertical adjustment.

.
. • Block the reference beam and check the counts for the
blocked reference beam.

Note: A maximum of 60 counts is admissible for the reference beam with

the reference beam blocked.

Not being able to get below 60 counts probably means the focus adjustment of
the parabola is not correct.

6.1 D2 Adjustment
Note: The final D2 adjustment should be performed after the detector
adjustment.

. • In the Read Window click on the Stop button.

.
. • Open the Slit Parameter Window and select the 0.2nm L slit.
.
. • In the Lamp Parameter Window enter 50 mA in the D2 Lamp
Current field and click on the Set button.
.
. • Open the Read Window and select:
Signal linBG
Read AA-BG

. • In the Read Window click on the Start button.

.
. • Adjust the focus and side-to-side movements of the D2 lamp
with the marked setscrews to get coincidence with the HCL
image and max. counts for both the sample and reference
beams of the D2 light.

40
7. Wavelength Calibration
. • Close the 4200BM and switch off the AAnalyst 600/700/800
spectrometer.
.
. • Re-install all optics covers.
.
. • Reinstall the burner compartment.

Note: A wavelength calibration must be done:

. • After exchanging or adjusting the wavelength drive.
.
. • After exchanging the Zero Power Ram, (IC 126 on the 4200
Controller Board) in which the values for correcting the linearity of
the wavelength drive are stored.

7.1 Conditions for the Wavelength Calibration

The conditions for a successful wavelength calibration are a well aligned:

• Mechanical and electrical zero order

• Optical zero order and

The following Single Element HCLs are required:

Lamp position 1 Cu (Copper 324,80 nm)

Lamp position 2 Ba (Barium 553.60 nm)
Lamp position 3 K (Potassium 766,50 nm)

7.2 Starting the Wavelength Calibration

Make sure the light beam is not blocked by the furnace. Remove the furnace
from the light path with the uninstall command of the ACU base module!

. • Switch on the AAnalyst 600/700/800 spectrometer.

.
. • From the Perkin-Elmer AA WinLab group start the 4200 Base
module by double clicking on the AA600/700/800BM icon.
.
. • Open the Align Lamp window. This will execute an initial

41
 instrument calibration. ( Zero Order )
.
. • Close the Align Lamp window.
.
. • Click on the wrench button in the tool bar of the SE BM.

• In the left hand side of the Optic Alignment window select the radio
button Wave.

• In Cycles type in 27. One sequence consists of 9 cycles:

cycle 1 Zero Order (Pos. 1)

cycle 2 Pos. 1 Copper 324,80 nm.
cycle 3 Pos. 2 Barium 553.60 nm.
cycle 4 Pos. 3 Potassium 766,50 nm.

cycle 5 Pos. 4 No lamp installed

42
 cycle 6 Pos. 5 No lamp installed
cycle 7 Pos. 6 No lamp installed
cycle 8 Pos. 7 No lamp installed
cycle 9 Pos. 8 No lamp installed

The more sequences that are run the better the corrected values. It is
recommended to use 27 cycles yielding 3 sequences.
• press the Start button in the Optic Alignment window.
The Lamps are switched on one after the other.
Below is a print out of the STDOUT.LOG file from a successful wavelength
calibration:
------------------------------------------------------------------------------------------------------------
------------------------------------------------------------------------------------------------------------

Optics = single beam

BGCorrection = Zeeman
LampTable = Lamp table THGA
load wavelength calibration from nvram
On = 1

N nominal / real steps

0 0 1
1 19488 19499
2 33216 33238
3 45990 46019

4 peaks, switch = 1
109: instrument error: "Undefined header" at command CNTRL:SENS:REV?
'
* Instrument connected *
Operation Mode = Flame
Optics = double beam
BGCorrection = D2
LampTable = Lamp table Flame
Start Wavelength Drive Test Cycles

Cycle-Lamp-Nomin-Peak - diff – Energy-Cap. Gain-Slit-Date Time

1 0 0.00 0.00 0.00 5983 32.0 0 0.2L 7.10.1999 08:48:22

2 1 324.80 324.98 0.18 3858 20.0 255 0.7H 07.10.1999 08:49:00
3 2 553.60 553.97 0.37 1404 12.0 255 0.2H 07.10.1999 08:49:30
4 3 766.50 767.03 0.53 7044 12.0 255 0.7H 07.10.1999 08:49:56

*** lamp 4: no current = disabled

*** lamp 5: no current = disabled
*** lamp 6: no current = disabled
*** lamp 7: no current = disabled
*** lamp 8: no current = disabled
10 0 0.00 0.00 0.00 5963 32.0 0 0.2L 07.10.1999 08:51:07
11 1 324.98 324.98 0.00 3916 20.0 255 0.7H 07.10.1999 08:51:32

43
12 2 553.97 553.97 0.00 1461 12.0 255 0.2H 07.10.1999 08:51:50
13 3 767.03 767.03 0.00 7096 12.0 255 0.7H 07.10.1999 08:52:07
19 0 0.00 0.00 0.00 5989 32.0 0 0.2L 07.10.1999 08:53:17
20 1 324.98 324.98 0.00 3940 20.0 255 0.7H 07.10.1999 08:53:42
21 2 553.97 553.97 0.00 1510 12.0 255 0.2H 07.10.1999 08:54:01
22 3 767.03 767.03 0.00 7109 12.0 255 0.7H 7.10.1999 08:54:20

• test end, 28 cycles, 0

errors*** Calibration results
***
lamp : num nominal- real-peak delta
steps nm steps nm steps nm
0 : 3 0 0.00 0 0.00 0 0.00

1 : 3 19488 324.80 19499 324.98 11 0.18

2 : 3 33216 553.60 33238 553.97 22 0.37

3 : 3 45990 766.50 46022 767.03 32 0.53

4: no correction result

5: no correction result

6: no correction result

7: no correction result

8: no correction result
* points found : 4***
Calibration results ***
lamp : num nominal -real- peak delta
steps nm steps nm steps nm
0 : 3 0 0.00 0 0.00 0 0.00
1 : 3 19488 324.80 19499 324.98 11 0.18
2 : 3 33216 553.60 33238 553.97 22 0.37
3 : 3 45990 766.50 46022 767.03 32 0.53
4: no correction result
5: no correction result

44
 6: no correction result
7: no correction result
8: no correction result
* points found : 4
store wavelength calibration to nvram
-------------------------------------------------------------------------------------------------------------
-------------------------------------------------------------------------------------------------------------
** Press Set WL-Table in the Optic Alignment window. (left
hand side of the window). The corrected values for
wavelength calibration are stored in the nvram (Zero Power
ram) on the 4200 Controller Board.

• Close the Base Module and switch off the spectrometer.

8 Final Test

8.1 Energy Check

After the wavelength calibration is completed, the actual wavelengths and
intensities should be checked:
. • Switch on the spectrometer.
.
. • Start the WinLab 32 for AA application by double clicking on the
icon.
.
. • Open the SE BM
.
. • Compare the table in the STDOUT window with the list below which
indicates a well initialized spectrometer.

Optics = double beam

BGCorrection = D2
LampTable = Lamp table Flame
load wavelength calibration from nvram
On = 1

N nominal / real steps

0 0 0

45
 1 19488 19495
2 33216 33224
3 45990 45992

4 peaks, switch = 1
* Instrument connected *

• Open the Align Lamp window to setup HCLs for different elements to
make sure, the wavedrive is correctly peaking the line!

8.2 Monochromator Resolution

. • Open Align Lamp Window in the Base Module. .
.
. • Setup Ni HCL and close Align Lamp Window.
.
. • Click on the Peak button in the tool bar of the SE BM.

. • Select ”From” 231.00 nm ”to” 233.00 nm.

46
.
. • Select Y-Axis: Counts; X-Axis: nm.
.
. • Check off DARK and LINE in Legend.
.
. • Apply Start and see Wavelength scan.
.
. • Put cursor over upper left corner of X-Y GRAPH for Ni Triplett
.
. • Depress right mouse button -Hold – and drag over the Ni Triplet
and release button.
.
. • Click right mouse button

To print the Graph:

. • Click Print in Peak window.
.
. • Go to File pull down menue and click Preview.
.
. • Go to File pull down menue and click Print .

8.3 Count Comparison Test AA700/800.

A count comparison test is to perform to make sure, that the sample- and the
reference-beam are aligned at their maximum position. If the maximas are not
matching each other, repeat chapter 6, Detector Adjustment on page 32.

. • Start running the SE BM only in Flame Mode.

.
. • Put in the first pos. the Cu HCL.
.
. • Open the Align Lamp window. (The wavelength initialisation occurs.)
.
. • Press the Set up button for the Cu HCL and warm up for 15 minutes.
.
. • Close Align Lamp Window.
.
. •Open the following windows:
.
Lamp Parameter
Slit Parameter
Wavedrive
Read

47
1. Select 0.7 H slit.

2. In Wavedrive window press Find max button.

3. In Turret Adjust press Find max. button.

4. In the Read window, select start.

5. Write down the Analytical Counts and Reference counts in: PEAKED with
REF. BEAM. Column.

6. In Read window press the Stop button.

7. Open the Test pull down menue and disable the Reference beam.

8. In Turret Adjust press Find max. button.

9. Open the Test pull down menue and enable the Reference beam.

10. In Read window press the Start button.

11. Write down the Analytical Counts and Reference counts in: PEAKED with
SAMPLE BEAM

12. In Read window press the Stop button.

Peaked with Ref. Beam Peaked with Sample Beam

Slit Ref. Counts Sample Counts Ref. Counts Sample Counts

0.7H

8.4 Specification Test

• Run the Performance Test (IPV Test) described in 4200003B.SIB
page 76.

10 Optical Path Drawing

The following parts are not assembled in the AAnalyst 600:
Fiber Optic
Beam Splitter
D2 Lamp Assy.
Mirrors E3 (for reference beam) and T3 (for D2 light)

48
49
 Numbers from Optical Alignment
(These need to be recorded & placed on the inside of the top cover)

Date:
Serial Number:
Slit Position (2.0 L):

Horizontal Vertical Horizontal Vertical

Lamp 1 Lamp 5
Lamp 2 Lamp 6
Lamp 3 Lamp 7
Lamp 4 Lamp 8
Furnace Flame

AS Pump Arm Slip:

Date:
Serial Number:
Slit Position (2.0 L):

Horizontal Vertical Horizontal Vertical

Lamp 1 Lamp 5
Lamp 2 Lamp 6
Lamp 3 Lamp 7
Lamp 4 Lamp 8
Furnace Flame

AS Pump Arm Slip:

50