SERVICE MANUAL

CE4200 SERIES
UV-VISIBLE DETECTOR

EDITION NO : 1
RELEASE NO : 5
DATE OF RELEASE : July 2009

APPLIES TO SOFTWARE RELEASE:

R0056

CE4200 from S/No. 151-101

CE4201 from S/No. 153-101
CE4300 from S/No. 161-101

PART NO: 4200 00 02

CECIL INSTRUMENTS LIMITED

SALES AND SERVICE

CECIL INSTRUMENTS LIMITED

MILTON TECHNICAL CENTRE
CAMBRIDGE CB24 6AZ

Telephone: +44 (0) 1223 420821

Fax: +44 (0) 1223 420475
Email: info@cecilinstruments.com
 SAFETY WARNING
THE FOLLOWING SERVICE INFORMATION IS ONLY TO BE USED BY
SUITABLY QUALIFIED PERSONNEL.

FOR YOUR OWN SAFETY, DO NOT PERFORM ANY SERVICING UNLESS YOU
ARE QUALIFIED TO DO SO.

WARNING:

MAINS VOLTAGE EXISTS AT SEVERAL POINTS WITHIN THE INSTRUMENT.

TAKE EXTREME CARE AND OBSERVE NORMAL SAFETY PRECAUTIONS
WHEN WORKING ON THE INSTRUMENT IF IT IS CONNECTED TO THE MAINS
AND THE TOP COVER HAS BEEN OPENED.

WARNING: DEUTERIUM ARC LAMP

THE LAMP EMITS CONCENTRATED ENERGY IN THE ULTRAVIOLET REGION.

PROTECT THE EYES FROM EXPOSURE TO THIS ENERGY.

WHEN THE LAMP IS OPERATING, THE ENVELOPE IS VERY HOT; ALLOW TO

COOL BEFORE HANDLING.

WARNING: DANGER OF SHORT CIRCUITING BATTERY

SOME MODELS HAVE A LITHIUM BATTERY BT1 MOUNTED ON THE MPU PCB,
NEAR EPROM U3. CARE MUST BE TAKEN TO ENSURE THAT EXPOSED
METAL PARTS OF THE I.C. EXTRACTION TOOL DO NOT MAKE ELECTRICAL
CONTACT WITH THE BATTERY.
Contents
1 INSTALLATION
1.1 LOCATION
1.2 STACKING
1.3 COLUMN AND INJECTION PANEL
1.4 CONNECTION TO MAINS POWER
2 MAINTENANCE
2.1 OPENING THE INSTRUMENT
2.2 CHANGING THE DEUTERIUM LAMP
2.3 ALIGNING THE DEUTERIUM LAMP
2.4 ABSORBANCE CALIBRATION
2.5 WAVELENGTH CALIBRATION
2.6 WAVELENGTH ADJUSTMENT
2.7 CLEANING THE FLOW CELL
2.8 RESOLUTION VALIDATION
2.9 STRAY LIGHT MEASUREMENT
3 FAULT FINDING
3.1 POWER FAILURE
3.2 ERROR MESSAGES
3.3 FAILURE OF START UP TEST AND CALIBRATION ROUTINE
3.4 NOT POSSIBLE TO SET ABSORBANCE ZERO
3.5 NOT POSSIBLE TO SET ZERO TRANSMISSION
3.6 UNSTABLE BASELINE, ABSORBANCE ZERO DRIFT
3.7 SUDDEN CHANGES IN BASELINE
3.8 WAVELENGTH NOT REPRODUCING
3.9 DEUTERIUM LAMP DOES NOT STRIKE
3.10 CHECKING THE DEUTERIUM LAMP CURRENT
3.11 REPLACING THE POWER TRANSISTOR
3.12 MAINS AND POWER SUPPLY FUSES
3.13 POWER SUPPLY TEST POINTS
3.14 REPLACING THE MPU AND DETECTOR INTERFACE PCB’S
3.15 REPLACING THE KEYBOARD
3.16 REPLACING THE POWER SUPPLY PCB
3.17 REPLACING THE PHOTO DIODE PCB
4 EXTERNAL CONNECTIONS
4.1 PARALLEL PRINTER
4.2 CONTROL I/O
4.3 SIGNAL OUT
4.4 SERIAL RS-232
5 PART NUMBERS
5.1 CELLS
5.2 SPARES AND CONSUMABLES
5.3 ELECTRICAL COMPONENTS
5.4 MECHANICAL COMPONENTS
6 FIGURES
FIGURE 1 THE FRONT PANEL
FIGURE 2 THE REAR PANEL
FIGURE 3 DEUTERIUM LAMP MOUNTING
FIGURE 4 LAYOUT OF 4200/4300 23 00 PHOTODIODE PCB
FIGURE 5 LAYOUT OF 4200 55 00 CONTROL PANEL PCB
FIGURE 6 LAYOUT OF 4200 75 00 DETECTOR INTERFACE PCB
FIGURE 7 LAYOUT OF 1201 80 00 HPLC MPU PCB

4200/4201/4300 Service Index : 2P Page 1

 FIGURE 8 LAYOUT OF 4200 66 00 DETECTOR POWER SUPPLY PCB
FIGURE 9 DETECTOR WIRING DIAGRAM
FIGURE 10 POWER SUPPLY WIRING DIAGRAM
FIGURE 11 FLOW CELL ASSEMBLY
7 SOFTWARE RELEASE UPDATE INFORMATION

8 HARDWARE CHANGES

9 TECHNICAL SPECIFICATION
9.1 SCANNING CE4300
9.2 DUAL WAVELENGTH CE4200
9.3 UV/VISIBLE DETECTOR CE4201

4200/4201/4300 Service Index : 2P Page 2

1 Installation
1.1 Location
The Cecil UV/Visible HPLC Detector is designed to provide the best possible
performance using available technology. To obtain the ultimate performance from the
instrument the environmental conditions should be as stable as possible. It should be
located in a position that is free of drafts with as near constant temperature as possible
and should not be placed in direct sunlight.

1.2 Stacking
The Detector is designed as part of the Adept series Modular HPLC System, as such
the individual components may be stacked with other units of the system. Note the
Detector(s) should always be on the top of the stack.

1.3 Column and Injection Panel

The column and injector are mounted on a panel which fits on the top cover just
behind the flow cell compartment. The panel is attached to the top cover with the two
screws supplied. A column cover is provided to shield the column from drafts when
working at very high sensitivity.

1.4 Connection to Mains Power

The unit is connected to the mains supply, using the mains cable provided, which fits
the power input socket on the rear panel. Note the mains voltage setting should be
adjusted to the correct voltage of the mains supply with the switch mounted on the
transformer housing on the rear left of the unit. The instrument leaves the factory set
for 230V operation. The instrument may now be switched on by the mains on/off
switch mounted on the rear panel of the instrument.

Note: The instrument performs a calibration procedure at switch on.

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2 Maintenance
2.1 Opening the instrument
a Remove the two M3 screws from the rear outer corners of the lid.

b Gently raise the lid from the rear of the instrument and slide it out backwards.

Note: To replace the lid, ensure that the front edge locates under the front lip of the
instrument prior to replacing the two screws.

c Remove the two counter sunk screws and washers from either end of the side
panels and remove the side panels.

2.2 Changing the Deuterium Lamp

The deuterium lamp is a specially selected lamp for use in the detectors and is fitted
with a life indicator, which indicates its hours of use.

Caution!
The lamp and lamp housing become very hot while the instrument is switched
on. Wait for these items to cool before attempting this maintenance procedure.
Do not touch the lamp while it is hot. Wear gloves when handling the lamp to
prevent oily residues being left on the envelope.
Caution!
The deuterium lamp emits harmful ultraviolet radiation. It is imperative that you
switch the instrument off before attempting this maintenance procedure.

a Switch the instrument off and wait for 30 minutes for the deuterium lamp and
housing to cool down.

b Loosen the lamp housing retaining screw (Figure 2 H) and lift off the housing.

c Carefully undo the spring anchor screw (A) to release the lamp retaining
spring (B).

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 d Using a small screwdriver, release the lamp wires from their clamping screws
and remove the lamp by lifting it upwards.

e Take the new deuterium lamp and feed the wires downwards and connect them
to the connector block as follows:

Connect the red wire to the centre terminal

Connect the blue wires to the two outer terminals

f The wires should be looped under and behind the lower end of the lamp.

g Lower the lamp so that its side arm rests in the V of the holder. Tension the
lamp retaining spring (B) over the side arm of the lamp using the spring
anchor screw (A) threaded through the end loop of the spring, and replace the
screw. The lamp should now be held in position.

h Adjust the lamp alignment as described.

2.3 Aligning the Deuterium Lamp

When a new lamp is fitted, small adjustments may have to be made to the lamp
height, and to lateral alignment to ensure maximum energy transmittance and
minimum noise and drift. This is a two-step process; a coarse alignment of the lamp
with the slot in the monochromator is followed by fine alignment to maximise the
energy shown on the electronic display.

Caution!
Be aware that the lamp and lamp housing become very hot while the instrument
is switched on.
Caution!
The deuterium lamp emits harmful ultraviolet radiation. It is imperative that you
wear special anti-UV goggles to avoid damaging your eyes.
Caution!
The HPLC detector has no internal parts that are serviceable by an operator. The
only reason for removing the casing is to align the deuterium lamp. The lid and
right side panel are removed during this procedure. Do not remove any other
parts of the casing. Do not touch any internal parts of the instrument.

2.3.1 Coarse Alignment

a Remove the right-hand side panel of the instrument by removing the two
screws and cup washers that secure it in the frame. Note the position and order
of all components, for later re-assembly.

b Remove the top cover of the instrument. Note the position and order of all
components, for later re-assembly.

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 c The right hand side monochromator end cover is secured by a single M3 screw
adjacent to the filter motor. Remove the screw and carefully lift out the end
cover. Note the position and order of all components, for later re-assembly.

d Use the techniques described for the fine adjustment (next section) to position
the lamp so that the beam falls symmetrically onto the centre of the slot (A).

e Replace and secure the lamp housing. Replace the instrument side panel.
Note: You can check noise and drift performance by using the dummy cell.

2.3.2 Fine Alignment

a Hold down the OFFSET key during power up. Release the OFFSET key when
the message ‘Service Calibration’ appears. The instrument powers up in
service mode.

b Fit the dummy cell into the holder in the cell compartment.

c Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Calibration’
and press E to select. The instrument will calibrate.

d Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Beam Mode’
and press E to select. Use the arrow keys to select 1, sample beam, and press
E.

The instrument will perform a zero and switch to transmittance readings. It

will display 100%. Allow it to warm up for 30 minutes.

e Remove the lamp housing by slackening the retaining screw (Figure 2, H) and
lifting off the housing.

f Refer to figure 3, slightly loosen the two locking screws (2).

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 g Use screw (1) to adjust the lamp height for maximum transmission (highest %
reading) as shown by the front panel display.

h Slightly loosen the locking screw (3).

i Use screw (5) to adjust the horizontal position of the lamp for maximum
transmission (highest % reading) as shown by the front panel display.

j Adjust the position of the lamp until the maximum energy is achieved. Tighten
the locking screws after the adjustment.

k Press the MODE key and select ‘Service Mode’. Use the arrow keys to select
‘Beam Mode’. Select 2, double beam

The instrument will perform a zero and switch back to absorbance readings.

2.4 Absorbance Calibration

The absorbance calibration may be checked using a set of neutral density filters that
are available from Cecil Instruments Limited with certificates traceable to NPL
standards. The instrument is also required to have the validation software option, and
a special cell holder to mount the filters in the sample compartment. Results are sent
to the parallel printer.

The following procedure can be used to check the absorbance accuracy:

a Using the Mode button select ‘Validation’, press E to select.

b Using the ARROW keys select ‘Abs Validation’, press E to select.

Using the ARROW keys, set the wavelength to either 510nm or 430nm. Press
E to select.

c Select the type of filter, the number of filters, and the tolerance.

d When prompted, enter the absorbances shown on the validation certificate.

e With the special cell holder fitted in the beam press the ‘Zero’ button.

f Insert each filter in turn and note the value. The results are sent to the printer
connected to the printer port in the rear panel of the detector.

Note: Insert the filters with the identifiers on the upper end and with the open side of
filter holders facing the rear of the instrument.
If validation is unavailable manually read the absorbance of the filters and
make a note.

Check the noted values against the calibration certificate taking into account the
certified uncertainty and the instrument tolerance for each filter.
i.e. Value ± uncertainty ± 0.005A or ±1%.

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2.5 Wavelength Calibration
The wavelength accuracy may be checked using a filter that is available from Cecil
Instruments Ltd, such as Holmium Filter part no. 0202 01 44. This filter can be
supplied, if required, with a test certificate which is traceable to NPL. A fee is charged
for this service. With validation software enabled see Section 2.5.1, if validation
software is unavailable refer to Section 2.5.2
Results are sent to a parallel printer, connected to the printer port on the rear panel of
the detector.

2.5.1 Note: The precise wavelength value(s) will depend upon the individual filter and its
calibration certificate.

The following procedure can be used to check the wavelength accuracy:

a Using the Mode button select ‘Validation’, press E to select.

b Using the ARROW keys select ‘WL Validation’, press E to select..

c Select the type of filter you are using, the tolerance and the number of
wavelengths.

d When prompted, enter the wavelengths shown on the validation certificate.

e When prompted, put the filter in the beam and press E.

f The results are sent to the printer connected to the printer port in the rear panel
of the detector.

g If the obtained results are outside specification then refer to section, 2.6
wavelength adjustment, to adjust the wavelength offset.

2.5.2 Note: The precise wavelength value(s) will depend upon the individual filter and its
calibration certificate.

Holmium Filter:- 334nm, 361nm, 419nm and 537nm.

Tolerance:- ±1.0nm, ±0,3nm uncertainty

The following procedure may be used to check the wavelength accuracy:

a Using the wavelength button drive the instrument to one of the above
wavelengths.

b Using the mode button select PEEK SEEK and E to enter, and follow the
prompts to run the reference.

c When prompted, insert the filter and press E. Note the reported value on the
screen and repeat the above for the wavelengths to be checked.

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 d The instrument’s tolerance is :- ±1.0nm, ±0,3nm uncertainty when compared
to the filters calibration. If the reported wavelengths are outside specification
then follow section 2.6 (below) to adjust the wavelength offset.

2.6 Wavelength Adjustment

If the wavelength calibration check indicates an error outside specification then the
following will allow the wavelength offset to be adjusted.

a Enter ‘Mode’ and select ‘Service Mode’ (see section 2.3.2)

b Under service mode enter ‘WL Offset’, the display will indicate the current
stored offset value in nm

c Enter a new offset value to bring the measured peaks within specification.

d Check the wavelength calibration again as in section 2.5 above.

2.7 Cleaning the Flow cell

The standard flow cell used with Detectors has a path length of 10mm and a volume
of 8µl. Cells of 10mm path length and 30µl volume and 5mm path length and 4µl
volume are also available which use the same method of construction as the 8µl cell.

a Refer to figure 11. Loosen the two compression screws F which connect the
inlet and outlet tubes.

b Use the cell key to remove one of the end plugs, A.

c Slide out the internal parts of the flow cell noting the order for re-assembly.

d Inspect the bore of the flow cell insert I to be clear of all forms of fibres or
partial obstructions and clean or replace as required.

e The windows should be cleaned with a solvent such as acetone or alcohol and
dried using a soft cloth or tissue.

Note: Take suitable precautions when using solvents.

Alternatively replace the windows with new ones. Once cleaned only handle
them by their edges, finger prints must not be left on the optical faces.

f After cleaning reassemble the cell following steps c to a above.

Note: It may be necessary to replace the window seals.

g Make sure that the p.t.f.e. washer D is fitted between the Kel-F insert and the
window at the outlet end of the cell.

h The end plugs should be tightened evenly ensuring that the insert remains
central with the flats correctly aligned i.e., normal to the inlet and outlet tubes.
These tubes may then be inserted and tightened using the fingers only.

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 i If the alignment should have moved in the assembly or not be accurate then
adjust the end plugs and retighten the fittings as required.

2.8 Resolution Validation

If the instrument has the validation software option, then the instrument optical
bandwidth can be measured. The technique involves scanning the narrow deuterium
emission line at 656nm and measuring the width of this peak at half its height.
The instrument prints the scan, and half the height of the peak is determined and
shown in %T along with the corresponding measured width in nm.

a Press the MODE key. The LED on the key flashes.

b Press the MODE key to run through the options in the menu until the
‘Validation’ option is displayed. Press the E key to select the option.

c Press the ARROW keys until the ‘Res Validation’ option is displayed. Press
the E key to select the option.

d The results are sent to the printer connected to the printer port in the rear panel
of the detector.

2.9 Stray Light Measurement

For this test the instrument is set to single beam mode, entered via the service mode
menu. It requires a 10mm pathlength silica cell containing the test solution of 10g/L
NaI, Sodium Iodide, dissolved in distilled or de-ionised water.
The instrument is also required to have a special cell holder to mount the 10mm cell
in the sample compartment.

a Hold down the OFFSET key during power up. Release the OFFSET key when
the message ‘Service Calibration’ appears. The instrument powers up in
service mode.

b Fit the special cell holder into the cell compartment.

c Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Calibration’
and press E to select. The instrument will calibrate.

d Fill a clean cell with distilled or de-ionised water then place the cell into the
beam, set the wavelength to 220nm.

e Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Beam Mode’
and press E to select. Use the arrow keys to select 1, sample beam, and press
E.

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 The instrument will perform a zero and switch to transmittance readings. It
will display 100%.

f Drain the cell and then rinse out and fill with the aqueous sodium iodide
solution. Insert a cell containing the sodium iodide solution into the beam, the
reading should be less than 0.02%.

g Press the MODE key and select ‘Service Mode’. Use the arrow keys to select
‘Beam Mode’. Select 2, double beam.

The instrument will perform a zero and switch back to absorbance readings.

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3 FAULT FINDING
3.1 Power Failure
a Check the mains lead is firmly plugged into both the instrument power socket
and the mains output socket and that the mains is switched on.

b Check the mains power fuse on the rear panel of the instrument and replace, if
failed, with the correct value fuse. Try to establish the cause of fuse failure.

c Check the fuse in the 13A plug, if such a plug is fitted.

If a replacement fuse blows at switch on the fault lies within the instrument.
The cause of the failure must be determined by a qualified engineer.

d If the instrument appears to start up but there is no display visible on the

screen use the up/down arrow keys to set the screen contrast.

3.2 Error Messages

Error Error Message

Category Cause
number
E1 Fatal Motor drive fault. Software error.
E2 Fatal Search for light failed. 1. Beam blocked.
Insufficient energy during the 2. Fault on ADC board.
calibration search for light. 3. Filter motor not operating.
E4 Fatal Pass code violation. Re-start the instrument.
Three consecutive incorrect attempts
at pass code entry.
E5 Transient Motor interrupt fail. 1. MPU board fault.
An expected motor step interrupt has 2. Software error.
failed to occur.
E7 Fatal Deuterium lamp failed to strike. 1. Replace deuterium lamp.
Problem with associated circuitry. 2. Replace deuterium lamp fuse.
3. Power supply board fault.
E8 Fatal ADC fault. 1. Connection between ADC and MPU
Excessive time taken to obtain the boards faulty.
next ADC reading. 2. ADC board fault.
3. MPU board fault.
E9 Fatal Fine wl calibration failed. 1. Wavelength motor unreliable
Unable to find deuterium emission 2. Align deuterium lamp.
line at 656nm 3. Sample absorbing at 656nm left in
beam.
E10 Fatal Fine wl calibration failed. 1. External light flooding cell
Wavelength calibration from compartment.
deuterium emission line disagrees 2. Fault with wavelength drive.
significantly with wavelength end stop 3. Deuterium lamp badly aligned.
sensor position. 4. Sample absorbing at 656nm left in
beam.
E11 Fatal Wavelength fault. Wavelength drive unreliable.
Wavelength motor end stop signal on
when expected to be off during coarse
wavelength calibration.

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 E17 Transient Serial communication error. 1. Check application software sets
Baud rate, parity, or data length correct serial parameters.
mismatch between instrument and 2. Check baud rate, parity, etc. are set
connected serial device. correctly.
E19 Transient Serial communication failed. 1. Software error.
2. MPU board fault.
E27 Fatal Motor calibration fault. Software error.
Excessive time taken for motor to stop
during motor calibration.
E28 Fatal Fine wl calibration fault. Sample absorbing at 656nm left in the
Absorbance zero failed at deuterium beam.
peak 656nm at the end of fine
wavelength calibration.
E30 Fatal Motor calibration fault. 1. ADC interrupts too fast.
Too many readings during motor 2. Software error.
calibration.
E31 Fatal Motor calibration fault. 1. Too few readings during motor
calibration.
2. Software error.
E33 Fatal Search for light failed. Excessive External light flooding cell compartment.
energy during the calibration search
for light.
E34 Fatal Deuterium lamp failed. 1. Problem with associated circuitry.
2. Change deuterium lamp fuse.
3. Replace deuterium lamp.
E35 Transient Deuterium lamp fault. 1. Problem with associated circuitry.
Deuterium lamp on when off 2. Power supply board fault.
requested.
E38 Transient Serial port time-out. Serial port may be disconnected.
It has taken longer than 15s to
transmit a character. Recovery is
attempted if the fault is rectified,
otherwise E93 is raised.
E40 Fatal Filter calibration fault. Insufficient 1. Filter mechanism jammed.
range of readings during filter motor 2. Filter motor unreliable.
calibration. 3. Incorrect or damaged filters.
E43 Fatal ADC or associated circuitry fault. 1. ADC board fault.
2. Problem with associated circuitry.
3. MPU board fault.
E44 to Fatal Microprocessor system failure. 1. MPU board fault.
E52 2. Software fault.
E54 Fatal Software error in memory allocation 1. Insufficient RAM available.
2. Software fault.
E60 Fatal RAM failed. 1. MPU board fault.
E61 Fatal PROM checksum failure. 1. MPU board fault.
2. PROM corrupted.
3. PROM incorrectly patched.
E62 Fatal Initialisation error. 1. Insufficient RAM available.
Insufficient software heap or stack? 2. Software fault.
E64 Fatal Filter calibration fault. 1. Filter drive unreliable.
Unable to identify filter pattern. 2. Damaged filter mechanism.
3. External light flooding cell
compartment.
4. Detached grating.

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 E66 Transient Problem with parallel printer. 1. Printer off-line.
It has taken longer than 15s to 2. Printer disconnected.
transmit a character. Recovery is 3. Printer out of paper.
attempted if the fault is rectified, 4. Faulty connection to printer.
otherwise E67 is raised. 5. MPU board fault.
E67 Transient Problem with parallel printer.
It has taken longer than 60s to
transmit a character. All data on this
channel has been discarded. (see E66)
E69 Transient Battery backed RAM corruption. Scan 1. Battery low.
data lost. 2. MPU board fault.
E71 Transient Battery backed RAM corruption. 1. Battery low.
Method data lost. 2. MPU board fault.
E74 Transient Unrecognised battery backed RAM 1. Battery low.
data deleted. 2. MPU board fault.
E75 Transient Battery low. 1. Replace battery.
2. Fault with associated circuit.
E79 Transient Zero T correction undefined. Contact your local service representative.
Default zero T correction applied.
E80 Transient Excessive zero T correction.
E84 Transient Real time clock fault. 1. Time and date not set.
2. Battery low.
E86 Transient Multiple keys pressed. Short circuit between keys.
During key test two or more keys
pressed simultaneously.
E87 to Fatal Microprocessor system failure MPU board fault.
E91
E93 Transient Serial port fault.
It has taken longer than 60s to
transmit a character. All data on this
channel has been discarded. (see E38)
E95 Fatal Calibration fault.
Unable to clear beam. During
calibration there has been a problem
with clearing the beam.
E97 Fatal Excess dark current. 1. External light flooding cell
During a transmittance zero the dark compartment.
current measured has exceeded one 2. ADC board fault.
quarter of the ADC full scale.

3.3 Failure of Start up Test and Calibration Routine

The major reason for such a failure are too little or too much energy reaching the
detector.

a Light beam partially or completely blocked. Error message E2 displayed.

To rectify clear the light beam and switch the instrument off and then on again
to restart the calibration routine.

b Too much light reaching the detector. Error message E33 displayed.
This may be the result of the flow cell compartment lid not fitted correctly or
the flow or dummy cell not being fitted into the cell compartment.

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 c Deuterium lamp failure during calibration.
The error message E7 displayed. Replace the lamp as in section 2.2 first
checking that the deuterium lamp fuse on the rear panel of the instrument.

3.4 Not possible to set Absorbance Zero

Try fitting the dummy cell in the first instance to determine if the fault is due to a
Detector failure of a sample problem.

a Light beam blocked, check flow cell is correctly fitted.

b Air bubbles in flow cell, These can often be dislodged by pinching the p.t.f.e.
outlet tube.

c Solvent highly absorbing at wave length selected. Use a different wavelength

or change the solvent.

d Dirty flow cell, Clean as described in section 2.7

e Check deuterium lamp alignment as in section 2.3

f Check recorder settings and connections.

3.5 Not Possible to set Zero Transmission

Flow Cell compartment lid not fitted correctly.

3.6 Unstable Baseline, Absorbance Zero Drift

a Air bubbles in the cell. Use only well degassed and filtered solvent. Check for
leaks or a dirty cell. See section 2.7

b Leaking cell or connection causing solvent vapour to enter the light path
causing absorption of energy from the light path.

c Well retained compounds which are eluted from the column after a
considerable delay. Turn off solvent flow, a steady baseline should result If
this is the cause purge the column at a high flow rate or use a stronger solvent.

d If the heat exchanger is not fitted between the column and the cell, temperature
instability could be the cause. Check for drafts etc.

e Failing Deuterium lamp. Replace if required. See section 2.2

f Check instrument stability with a dummy flow cell.

g Check recorder and connections, change recorder and or connections.

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3.7 Sudden Changes in Baseline

a Air bubbles entering flow cell, see 3.6a above.

Check for small leaks that may allow air to enter but not allow solvent to
escape.

b Unstable deuterium lamp. This often gives rise to fairly regular steps in the
baseline. The effect will still be present when the solvent flow is turned off or
the dummy cell is fitted.
Sometimes the lamp can be corrected by switching off and on a few times in
succession.

c Sudden changes in pressure or flow rate of solvent delivery. Check pump

performance.

d Remove flow cell, fit and run with dummy cell.

3.8 Wavelength Not Reproducing

a Dirt on edge of cam. Wipe clean with a cloth.

b Cam arm return spring not functioning properly. Check visually and also
gently lift arm off cam to test spring tension.

c Check the belt tension is not too loose, check pinion gear is locked tight to
drive shaft.

3.9 Deuterium Lamp Does Not Strike

If the D2 indicator is not displayed steady on the screen within a few minutes of
switching on, check the following:-

a Check that the deuterium lamp is in fact off. Remove the cover of the lamp
house. If the lamp glows with a bright blue light (DANGER to eyes from UV
radiation) it has struck and the indicator circuit has failed.
If a red glow is visible inside the lamp structure, this shows that the lamp
heater is on.
Proceed to section b below, if no light can be seen at all proceed to section c.

b The lamp should operate for 1 year from the date of supply. If the lamp is
older, then either replace the lamp, see section 2.2, or check the fuse for the
deuterium supply mounted on the rear panel.

If it has blown there is a fault on the PCB, a short circuit in the deuterium lamp
or a faulty power transistor. Locate the fault by inspection or replacement.

If the fuse is OK then check the HT supply, see section 3.13.f

4200/4201/4300 Service S3 : 9P Page 5

 If the HT supply is OK there may be a fault in the Deuterium lamp or the PCB
is faulty, replace the lamp, then the PCB.

c If no glow can be seen from the deuterium lamp, the heater is open circuit or
there is a fault in the power supply. Either the lamp must be replaced, see
section 2.2, or it may be possible to cure the fault by changing the deuterium
lamp power supply board.

Check the heater winding and rectifier as follows :-

There should be about 12 volts AC on the orange wires on pins 7 and 8 of

connector PL2 on the power supply PCB (terminals 11 and 12 on the
transformer) and about 11 volts mean DC on the outer terminals of the
deuterium lamp connector block, (wires 73 and 76).

3.10 Checking the Deuterium Lamp Current

Measure the DC voltage from TP5 ( a test pin) to TP1 (a test pin) on the power supply
PCB. With the lamp burning adjust VR5 for a reading of 3.83 volts DC.

3.11 Replacing the Power Transistor

The MJN11016 deuterium lamp current regulating transistor is mounted on the heat
sink behind the instrument.

Switch off and disconnect the instrument from the mains supply.

a Remove the four screws that hold the transformer housing to the rear panel

b Slacken the two M4 cap head allen screws (that secure the heat sink to the
transformer housing) approximately two turns then slide the heat sink down
off its fixing.

c Remove the two screws that secure the transistor and then unsolder the two
remaining wires, noting the connections and the 2K2 resistor in the base lead.

d Ensure that the new transistor is insulated from the heat sink by top hat
insulators and a silicone rubber insulator or equivalent. Check the insulation
with a meter. Re-solder the wire connections as noted.

e Re-assemble by reversing the above instructions.

3.12 Mains and power supply fuses

Both fuses are fitted on the rear panel on the instrument.

a The mains fuse is type T3.15A

b The deuterium lamp fuse is type F0.5A

4200/4201/4300 Service S3 : 9P Page 6

3.13 Power supply test points
There are several test points on the power supply pcb, refer to figure 8

All readings are relative to Test Point 1 (TP1), 0 volts

a TP2, +5 volts

b TP3, +12 volts

c TP4, -12 volts

d TP5, +3.83 volts, see note below.

e There is also a +16 volt DC unregulated supply, this can be measured on Pin 1
of connector PL1, again relative to TP1, 0 volts.

f The Deuterium lamp running voltage (HT supply) can also be checked by
measuring the voltage on wire 46, either on Pin 3 of connector PL4 or on the
fuse holder for Fuse FS2, again relative to TP1, 0 volts. This voltage should
be ~120V D.C. (max.140V, min 100V).
Warning this voltage can be lethal. Refer to Figures 8 & 10.

Note: only the voltage measured on TP5 is adjustable, see section 3.10 above.

3.14 Replacing the MPU and Detector Interface PCB’s

Switch off and disconnect the instrument from the mains supply.

a Remove the lid and side panels as in 2.1 above

b Remove the ribbon cables (PL 1 and PL8) from the Keyboard and the ADC

c Remove the screw terminal plugs (PL 10 and PL11) from the Power Supply

d Remove the 6 pin connectors (PL6 &7) from the Wavelength and Filter
motors

e Remove the Ribbon Cable (PL5) from the printer socket

f Remove the two hex nuts that secure 9 way D plug (PL9) to the rear panel

g Remove the 4 M3 nuts and washers that hold the interface and MPU PCB’s to
the left side of the monitor chassis and lift them out carefully.

Note: the MPU plugs into the interface PCB and can be separated once the interface
is clear of the instrument

h Replace the PCB’s by reversing the instructions a to h above.

4200/4201/4300 Service S3 : 9P Page 7

3.15 Replacing the Keyboard

Switch off and disconnect the instrument from the mains supply

a Remove the lid and side panels as in 2.1 above.

b Remove the 4 screws with shake proof washers from each corner of the front
panel and ease the panel forward.

c Detach the keyboard from the front panel by removing the 4 screws on the rear
face of the front panel that attach the control panel to the front panel, noting
the earth bond wire under one of the screws. Release the ribbon cable that
links it to the interface PCB (PL1).

d Replace the keyboard by reversing steps a to c above.

e Reset the screen contrast by use of the up/down keys after the instrument has
been switched on.

3.16 Replacing the Power Supply PCB

Switch off and disconnect the instrument from the mains supply

a Remove the lid as in 2.1 above

b Remove the 4 screws and shake proof washers that secure the rear panel to the
rear of the instrument and ease the panel way from the rear chassis.

c Remove the four power supply plugs (PL1, PL2, PL3 & PL4) from the PCB.

d Release the two spring clips that secure the PCB to the inner rear panel then
ease the PCB out of the back of the monitor.

e Replace the PCB by reversing the instructions a to e above.

3.17 Replacing the Photo Diode PCB

Switch off and disconnect the instrument from the mains supply

a Remove the lid and side panels as in 2.1 above.

b Remove the 4 screws with shake proof washers from each corner of the front
panel and ease the panel forward.

4200/4201/4300 Service S3 : 9P Page 8

 c Remove the two M3 screws that secure the cover over the Photo Diode PCB,
in front of the cell compartment

d Remove the four M3 nuts and washers that secure corners of the PCB

e Ease the PCB forward slightly and then unsolder the two wires connected to
terminals P3 & P4, sleeved Red & Blue, the connections from the reference
photo diode, noting the orientation. Refer to Figure 4

f Continue to ease the PCB forward, carefully extracting the sample photo diode
from it’s mounting. Note, the sample photo diode is soldered to terminals P1
& P2 on the rear of the PCB

g Remove the ribbon cable from connector PL1.

h Replace the PCB by reversing the above procedure.

4200/4201/4300 Service S3 : 9P Page 9

4 External Connections
4.1 Parallel printer
25 pin D socket (standard connection)

4.2 Control I/O

8 pin connector Pin 1
pin 1 0V
pin 2 IN 1 Start signal
pin 3 IN 2 Stop signal
pin 4 IN 3 Autozero signal
pin 5 0V
pin 6 OUT 1 Ready signal
pin 7 OUT 2 Start signal
pin 8 OUT 3 Stop signal
Pin 8

4.3 Signal out

4 pin connector
2
pin 1 Out A (channel 1) 4
pin 2 0V
pin 3 Out B (channel 2) 3
pin 4 0V 1

4.4 Serial RS-232

9 pin plug (standard null-modem connection)
pin 1 N/C
pin 2 RXD
pin 3 TXD
pin 4 DTR
pin 5 0V
pin 6 N/C
pin 7 RTS
pin 8 CTS
pin 9 N/C

4200/4201/4300 Service S4 : 1P Page 1

5 Part Numbers
5.1 Cells

8µL, 10mm pathlength flow cell 1200 07 70

4µL, 5mm pathlength flow cell 1200 07 72
30µL, 10mm pathlength flow cell 1200 07 71
1mm pathlength preparative cell 1200 07 73
Spectrosil end windows for cells 1200 48 04
Compression screws for tubing 1200 48 05
Inlet and outlet tube P.T.F.E. 0.012 inch bore 1200 48 06
Key for assembling cell 1200 49 00
Dummy cell 1200 48 00
Filter holder 4200 48 00

5.2 Spares and Consumables

Operating Manual (CE4200, CE4201) 4200 00 01

Operating Manual (CE4300) 4300 00 01
Service Manual 4200 00 02
Short Form Instructions 4100 00 03
Deuterium Lamp 1200 01 42

5.3 Electrical Components

Fuse T3.15A HRC (mains fuse FS1) 5505 01 56

Fuse F0.5A quick blow (deuterium lamp FS2) 292 61 12
HPLC MPU (fitted with 9020 80 76 3V7 battery) 1201 80 00
Detector control panel PCB assembly 4200 55 00
Photodiode PCB (CE4200, CE4201) 4200 23 00
Photodiode PCB (CE4300) 4300 23 00
Sample Detector Assembly (CE4200, CE4201) 4200 12 00
Sample Detector Assembly (CE4300) 4300 12 00
Detector PSU PCB 4200 66 00
Detector Interface PCB 4200 75 00
LCD module 4 line x 20 char 1201 55 07
BDX63C/MJ11016 power transistor (deuterium supply) 2202 40 15
Toroidal mains transformer 4200 25 01

5.4 Mechanical Components

Spec ‘B’ Sample lens 202 04 07

105 tooth belt 4200 14 32
Stepper motor 7.5 degrees (filter) 4200 14 34
Stepper motor 1.8 degrees (wavelength) 4200 14 36
Cell compartment entrance window 505 04 16
Spec ‘B’ lens 1200 03 05

4200/4201/4300 Service S5 : 1P Page 1

6 Figures
Figure 1 The Front Panel
The front panel of the detector is shown below. Note the Rheodyne valve and column
mounting panel are optional accessories.

A Electronic display C Rheodyne valve

B Keypad D Column mounting panel
E Column

Keys

and  Use these keys to scroll through menu options and to increase or decrease a value
( ARROW keys) shown on the electronic display. They are also used to adjust the screen contrast.
E (EVENT CANCEL) This key provides three functions. It is used to accept any entered or default value. It
is also the event marker. When you press the key at any time, other than to accept or
cancel a value, it causes a pen deflection of an attached chart recorder. Holding the
key pressed clears any entered value to 0.
MODE Press this key to display the ‘mode’ menu options, which are mainly concerned with
configuring the detector for use, and setting up pump gradients.
METHOD Press this key to display the ‘method’ menu options, where you can save current
settings and parameters into methods.
SCAN If you have purchased the scanning option (available for the CE 4200 only), use this
key to display the ‘scan’ menu options. They allow you to scan a range of
wavelengths to find the one that is most appropriate for the sample that you are
measuring.
ZERO Zeros the instrument electronic display and signal output.
OFFSET Allows you to change the offset that has been set for the signal out port.
W’LENGTH Allows you to set the wavelength.
RANGE Allows you to change the range that has been set for the signal out port.

4200/4201/4300 Service S6 : 11P Page 1

 Figure 2 The Rear Panel

A B C E F G

H I J K L M

A Deuterium lamp housing H Lamp house retaining screw

B Heat sink
C Transformer housing
D Power supply voltage selector
E Printer port
F Power On/Off Switch
G Control inputs and outputs
I Deuterium lamp fuse
J Power Inlet Socket
K Power supply fuse
L Serial port
M Signal out

4200/4201/4300 Service S6 : 11P Page 2

 Figure 3 Deuterium Lamp mounting

1. Height adjustment thumb screw

2. Height adjustment locking screws
3. Horizontal adjustment locking screw
4. Lamp holder mounting screws
5. Horizontal adjustment thumb screw
6. Spring anchor thumb screw
7. Lamp retaining spring

4200/4201/4300 Service S6 : 11P Page 3

 Figure 4 Layout of 4200/4300 23 00 Photodiode PCB

4200/4201/4300 Service S6 : 11P Page 4

 Figure 5 Layout of 4200 55 00 Control Panel PCB

4200/4201/4300 Service S6 : 11P Page 5

 Figure 6 Layout of 4200 75 00 Detector Interface PCB

4200/4201/4300 Service S6 : 11P Page 6

 Figure 7 Layout of 1201 80 00 HPLC MPU PCB

4200/4201/4300 Service S6 : 11P Page 7

 Figure 8 Layout of 4200 66 00 Detector Power Supply PCB

4200/4201/4300 Service S6 : 11P Page 8

 5 4 3 2 1

HPLC MONITOR INTERFACE (SM)

MONITOR PHOTODIODE REAR PANEL CONNECTIONS

PL5 4200-92-00
PARALLEL PRINTER PARALLEL PRINTER
PL1 4200-91-00 PL8
MONITOR I/F ADC

4200-23-00 SK1 SK1-1 OUTA

ANALOGUE OUT
SK1-2 0V
D D
SK1-3 OUTB
SK1-4 0V

PL3 PL3-1 0V

4200/4201/4300 Service
MONITOR CONTROL PANEL DIGITAL I/O
PL3-2 IN1
PL3-3 IN2
PL3-4 IN3
PL3-5 0V
PL1 4200-90-00 PL1 PL3-6 OUT1
MONITOR I/F CONTROL PANEL
PL3-7 OUT2
PL3-8 OUT3

4200-55-00 PL9 PL9-1 NOT CONNECTED

RS-232 SERIAL
PL9-2 RXD
PL9-3 TXD
PL9-4 DTR
PL9-5 0V
PL9-6 NOT CONNECTED
PL9-7 RTS
C C
POWER PL9-8 CTS
PL9-9 NOT CONNECTED

HPLC MPU

4200-94-00
PL3-1 20 PL11-1 PL2 SK1
+12V +12V DIN41612 CONNECTOR DIN41612 CONNECTOR

PL3-3 21 PL11-3
0V 0V
NOTE:
PL3-4 22 PL11-4 THE HPLC MPU IS MOUNTED DIRECTLY
-12V -12V

S6 : 11P
ONTO THE HPLC MONITOR INTERFACE
PL3-5 23 PL11-5
0V 0V
VIA THE DIN41612 CONNECTORS.
PL3-6 24 PL11-6
D2ENABLE D2ENABLE

PL3-7 25 PL11-7
D2STRIKE D2STRIKE 1201-80-00

PL3-8 26 PL11-8
D2ON D2ON

B PL3-9 27 PL11-9 PL6 B

D2HEATON D2HEATON W/L MOTOR[1..4]

PL3-10 28 PL11-10
BRESET BRESET

M1
4200-93-00 WAVELENGTH MOTOR
PL1-1 30 PL10-1
VMOTOR VMOTOR
PL7 1 P+
FILTER MOTOR[1..4]
PL1-2 31 PL10-2
0V 0V
2 P-
10 PL1-3 32 PL10-3
L VCC VCC
M2
60 PL1-4 33 PL10-4 FILTER MOTOR 5 Q+
N VCC VCC

PL1-5 34 PL10-5 1 P+ 6 Q-
0V 0V

PL1-6 35 PL10-6 3 P-
0V 0V

5 Q+
W100I001.SCH
6 Q-
PL1 54 1 4200-75-00

A 1 A
3 4 FRONT PANEL
2
50 2 5 53 1
3
1 6
MAINS 51 LEFT SIDE FRAME CECIL INSTRUMENTS LIMITED
INPUT 52 1

0
FILTER Title
RIGHT SIDE FRAME CE 4200 MONITOR WIRING DIAGRAM
BASE PLATE BACK PANEL
Size Document Number Rev
B 4200-000 W000I001.SCH 1

Date: Tuesday, November 27, 2001 Sheet 1 of 2

5 4 3 2 1

Page 9
Figure 9 Detector Wiring Diagram
 5 4 3 2 1

1
LIFE INDICATOR
(HOURS)

2
D D

4200/4201/4300 Service
DEUTERIUM LAMP
MONITOR POWER SUPPLY

FS2

PL4-3 46 72
D2 RUN
T1

2
3
TRANSFORMER F0.5A TB1

PL4-10 73 1 2 BLUE
D2 HEATER
BROWN 4 42 RED PL2-1
16V AC1
PL4-1 49 1 2 RED
D2 ANODE
41 RED PL2-2
16V AC2
SW2 PL4-9 76 1 2 BLACK
D2 TRCOL
MAINS 32 YELLOW PL2-3
95V AC1
VOLTAGE 77
15 2A 230V SELECTOR 3 31 YELLOW PL2-4
95V AC2
2 R1
12 2B WHITE 2 22 GREEN PL2-5 PL4-8 75 TR1
15V AC1 D2 TRBASE
C1 MJN11016
1A 21 GREEN PL2-6 2k2
15V AC2
C 220nF 1 BLACK C
250V X2 1B 12 ORANGE PL2-7 PL4-7 74
13V AC1 D2 EMITTER
115V
BLUE 1 11 ORANGE PL2-8
13V AC2

PL3-1 20
+12V +12V

4200-25-01 PL3-3 21
0V 0V

PL3-4 22
-12V -12V
12
61 PL3-5 23
0V 0V

SW1 PL3-6 24
D2ENABLE D2ENABLE
MAINS

S6 : 11P
ON/OFF PL3-7 25
D2STRIKE D2STRIKE

11 PL3-8 26
D2ON D2ON

PL3-9 27
D2HEATON D2HEATON
FS1
T3.15A PL3-10 28
BRESET BRESET\

B B

PL1-1 30
+16V VMOTOR
10
L
PL1-2 31
0V 0V (16)
60
N
PL1-3 32
+5V VCC

PL1-4 33
+5V VCC

PL1-5 34
0V 0V

PL1-6 35
0V 0V

4200-66-00

A A

CECIL INSTRUMENTS LIMITED

Title
CE 4200 MONITOR WIRING DIAGRAM

Size Document Number Rev

B 4200-100 W100I001.SCH 1

Date: Tuesday, November 27, 2001 Sheet 2 of 2

5 4 3 2 1

Page 10
Figure 10 Power Supply Wiring Diagram
 Figure 11 Flow cell assembly

E
OUTLET
C
H
A B D F C B A

G I
F

INLET E

A End plug F Compression screw

B P.T.F.E. washer G Cell body
C Window H Red marker
D Thin P.T.F.E. washer I Insert
E P.T.F.E. tube

4200/4201/4300 Service S6 : 11P Page 11

7 Software Release Update Information

R0048: Initial production release.

R0049: Spanish and Portuguese added.

R0050: Trigger scan and integration added.

R0052: Peek Seek added under mode

R0054: Digital CSM & PowerStream released

R0056: Release of new Adept Auto Sampler

4200/4201/4300 Service S7 : 1P Page 1

8 Hardware Changes

Release R0050 S/No. 161-101: Introduce CE4300.

4200/4201/4300 Service S8 : 1P Page 1

9 Technical Specification
9.1 Scanning CE4300
Scanning Specification
Wavelength Range
Optical Bandwidth
Digital Resolution
Wavelength Accuracy
Absorbance Range
Scan Speeds
Baseline Flatness
Second Order Filter
Noise
Drift
Overlayed Scans
Scan Trigger
Number of Peak Scans
Scan Storage
Multiple Scans of a Peak
Used with DataStream Software
Variable Wavelength Detection
Wavelength Range
Wavelength Accuracy
Wavelength Reproducibility
Absorbance Range
Absorbance Linearity
Optical Bandwidth
Dual Wavelength Operation
Wavelength Programming
Noise
Drift
Noise – Dual Wavelength
Drift – Dual Wavelength
General Specification
Optical System
Monochromator
Straylight
4200/4201/4300 Service
190-700nm
4nm – constant
1nm
1nm
0.001 to 3A
Up to 1000nm/s – UV scan in 200ms
± 0.002A over most of range
Fast automatic insertion at 370nm
< ± 0.7 x 10–5 A, 230nm, 2 s T/C, using air test cell
< ±0.7 x 10–4 A/h, 230nm, at equilibrium using air test cell
Up to 8 scans may be overlayed
At start of peak, set absorbance value, point of inflection or
at peak
Up to 100 peaks for a single elution
Up to 100 scans password protected
Up to 8 scans of any peak
Data to PC, PC control, scan triggering, Lambda max. scans,
peak purity and purity calculation, graphical presentation,
overlay, normalisation, disk storage, library store and search,
transfer to Excel etc and many other functions.
190-700nm
1nm
± 0.1nm
0.001 to 3A
Better than 1% 0.001 – 2A
4nm – constant
Simultaneous measurement at 2 wavelengths and ratio
Measurements at up to 6 different wavelengths with a time
program and selectable sensitivity
± 0.5 x 10–5 A at 230nm, 2s T/C, using air test cell
±0.3 x 10–4 A at 230nm at equilibrium, using test cell
± 2 x 10–5 A at 250/280nm
± 2 x 10–4 A at 250/280nm, constant temperature
Double Beam
Littrow with 1200 l/mm holographic grating
< 0.02% p.t.p. at 220nm and 340nm
S9 : 3P Page 1
Detectors Two Silicon Diodes
Lamp Deuterium lamp with elapsed time indicator
Taper Beam Optics All energy collected to minimise RI effects
Self Test Automatic at switch-on; continuous monitoring of key
functions
Autozero By control panel or external trigger
Time Constant 0.1 sec – 10 sec, selectable
Wavelength Calibration Automatic at switch-on using deuterium emission line.
Display Four-line display of parameters, menus, prompts,
wavelength, absorbance etc.
Fast Number Entry By use of special number keys
Event Marking From keypad or by external trigger
Method Storage For up to 30 methods, security protected
Automatic Power-Up/Off Pre-programmed to save warm up time
Outputs Two absorbance outputs, or absorbance and absorbance
ratio. Data available to PC.
Inputs Event, autozero
Size and Weight 365 x 290 x 140mm, 17kg
Power Requirements 115V or 230V, 50/60Hz, 50VA

9.2 Dual Wavelength CE4200

All specifications as for the CE 4201 with the following additional specifications

Noise ± 0.35 x 10-5 A peak to peak, 220nm, 2 sec time constant

Drift
Dual Wavelength Operation
Noise, Dual Wavelength
Drift, Dual Wavelength
Programmed Operation
± 3 x 10-5 A/hour at equilibrium
Simultaneous measurements at 2 wavelengths.
Output of absorbance ratio for the 2 wavelengths
± 2 x 10-5 A peak to peak, 230/280nm, 1s time constant
± 1 x 10-4 A/hour at constant temperature
Programs may be entered to change parameters, operating
modes and select up to 6 different wavelengths in any time
sequence.

9.3 UV/Visible Detector CE4201

Optical System Double beam with 1200 lines/mm grating monochromator

Wavelength Range 190-700nm
Wavelength Accuracy ± 1nm
Wavelength Precision ± 0.1nm, single wavelength mode
Optical Bandwidth 8nm
Straylight < .02%, 220nm
Lamp Deuterium lamp with elapsed time indicator
1500 hours or 1 year warranty
Absorbance Range 0.001 to 2A full scale in 11 ranges
Time Constant 0.1, 0.2, 0.5, 1, 2, 5 and 10 seconds
Display Four-line display of parameters, menus, prompts,
wavelength, absorbance etc.

4200/4201/4300 Service S9 : 3P Page 2

Fast Number Entry By use of special number keys
Event Marking From keypad or by external source
Method Storage For up to 30 methods, security protected
Second Order Filter Fast automatic insertion at 370nm
Noise ± 1 x 10-5 A peak to peak, 220nm, 2 second time constant
Drift ± 1 x 10-4 A/hour at constant temperature
Linearity Better than 1%, 0.001 – 2A
Automatic Power-up/Off Pre-programmed from keypad to save warm up time
Sample Flow Cell 8µL x 10mm pathlength – normal
4µL x 5mm pathlength
Wetted surfaces PEEK, Teflon and Fused Silica
Other cells available
Refractive Index Sensitivity < ± 5 x 10-4 A, water to methanol at 350nm
Flow Rate Sensitivity < ± 3.5 x 10-3 A, for flow change 1-2mL/min, 300nm
Outputs Two absorbance outputs 0-1V full scale, 20 bit resolution.
Parallel printer port, Epson ESC/P protocol
Stop/Start of externally connected modules
All data available to PowerStream System Manager.
Inputs Event, autozero, stop/start
Size and Weight 365 x 290 x 140mm, 17kg
Power Requirements 115V or 230V, 50/60Hz, 50VA

4200/4201/4300 Service S9 : 3P Page 3