PCE-210E Ver. 5.

45N

8-5 RS-232C Output

●Output format

Output Order Item Name Output digits Output code

1 STX STX
2 Sample No. 5 digits 00000
3 Date 10 digits yymmddhhmm
4 WBC 5 digits 000.0
5 LY 5 digits 000.0
6 MO 5 digits 000.0
7 GR 5 digits 000.0
8 LY% 5 digits 000.0
9 MO% 5 digits 000.0
10 GR% 5 digits 000.0
11 RBC 5 digits 00.00
12 Hgb 5 digits 00000
13 HCT 5 digits 000.0
14 MCV 5 digits 000.0
15 MCH 5 digits 000.0
16 MCHC 5 digits 00000
17 RDW 5 digits 000.0
18 PLT 4 digits 0000
19 PCT 5 digits 0.000
20 MPV 5 digits 000.0
21 PDW 5 digits 000.0
22 WBC Histogram 3 digits X 200 data 000 X 200
23 RBC Histogram 3 digits X 200 data 000 X 200
24 PLT Histogram 3 digits X 200 data 000 X 200
25 ETX ETX

Forwarding Speed : 9,600 bps

Start bit : 1 bit
Data bit : 8 bit ASCII Code
Parity bit : None
Stop bit : 1 bit

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 PCE-210E Ver. 5.45N

9 MEASUREMENT PRINCIPLE

9-1 Electric Resistance (Detection part)

Measurement method of PCE-210 is electric resistance.

The following figure explains Electric Resistance. Blood cells float in electrolyte solution
(Diluent Solution) and when each blood cells which is regarded as insulator pass through
Orifice, resistance will changed. This method counts the change. Depending on the size of
resistance, it is counted as RBC / WBC / PLT and electric noise.

During measurement of samples, electric current passes between the electrode(+) inside and
the electrode(-) outside in Chamber through Orifice. A sample in Chamber is suctioned by
regular negative pressure. Volume of resistance changes depending on volume of blood cells.

Blood cells are sorted out depending on size and volume. By diluting a sample properly, RBC
and PLT are differentiated as volume of RBC is different with that of PLT.

WBC can not be counted properly just by dilution because the number of WBC is much smaller
than other parameters such as RBC and PLT. So Lyzing Solution must be put into a sample to
clear RBC before WBC count.

WBC can be classified into some subordinate cells such as Lymphocyte and Granulocyte.
Lyzing Solution also affects WBC itself, and it makes WBC contracted. By this action of Lyzing
solution, these WBC subordinate cells can be discriminated by the volume of each cell with
the method of Electric Resistance.

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