CONCLUSION

In conclusion , The standard solution of phosphorus can be prepared by dilution of

stock solution. The stock solution was diluted into 5 standard solution. The concentration of
standard 1 is 0.2 mg/L , following by 0.4 mg/L for standard 2 , 0.6 mg/L for standard 3 , 0.8
mg/L for standard 4 and 1.0 mg/L for standard 5. Then, allow 30 minutes for all the solution
for colour development. Next , the average absorbance for 5 standard solutions is 0.105 for
standard 1 , 0.207 for standard 3 , 0.390 for standard 3 , 0.493 for standard 4 and 0.547 for
standard 5. The standard deviation for 5 standard solutions is 0.004 for standard 1 , 0.006 for
standard 2 , 0.002 for standard 3 , 0.003 for standard 4 and 0.015 for standard 5. The
absorbance for all the solutions calculated by UV-Vis spectrophotometer. To find the

gradient of the equation , we used m=

(
Σ x i y i−
n )
Σ xi × Σ y i

. The value Σ x i is 3.00mg L- and

2
2 ( Σ xi )
Σ xi −
n
2
the value Σ y i is 1.742. The value of Σ x i and Σ x i y i is 2.2 and 1.2792. From all the value , we
can get the value of gradient ,m. The linear equation for y=0.5831x-0.00155 by using least
square method. From the graph , it can be seen that , the higher the concentration of solution ,
the higher the absorbance. Beside that , an unknown sample also has been prepared by the lab
instructor.The absorbance and the concentration of unknown sample are calculated from the
linear equation. After the calculation , the absorbance of the unknown is 0.410 and the
corresponding concentration is 0. 7058mg L-1