Capillarys 3 TERA - Service Manual (v1.15P1!22!03-2019)

CAPILLARYS 3 TERA & OCTA
SERVICE MANUAL
Capillarys 3 TERA & OCTA – Service Manual Page 1 of 120 v1.15 P1 – Date: March 18th, 2019
Document history summary:
Version Author Date

1.04B SEBIA Service Department February 17th, 2016
1.05A SEBIA Service Department April 11th, 2016
1.06B SEBIA Service Department April 20th, 2016
1.08A SEBIA Service Department August 08th, 2017
1.09 SEBIA Service Department September 25th, 2017
1.09 SEBIA Service Department September 27th, 2017
1.15 SEBIA Service Department August 20th, 2018
1.15 P1 SEBIA Service Department August 28th, 2018
1.15 P1 SEBIA Service Department December 14th, 2018
1.15 P1 SEBIA Service Department January 28th, 2019
1.15 P1 SEBIA Service Department March 18th, 2019
1.15 P1 SEBIA Service Department March 22th, 2019
LATEST UPDATES OF THE SERVICE MANUAL – Version 1.15 P1 –March 18th, 2019
- FILLING POSITION – Page 74

- HBA1C CAPILLARY TUBES – Pages 93 to 94
- SCHEMATICS – Pages 101 to 115
LATEST UPDATES OF THE SERVICE MANUAL – Version 1.15 P1 –March 22th, 2019
- SCHEMATICS – Page 115
NOTE: most of the pictures and screenshots are common to both instruments.
Except for the screenshots of the optical menus:
CAPILLARYS 3 TERA: 13 channels (reference + 12 capillaries).
CAPILLARYS 3 OCTA: 9 channels (reference + 8 capillaries).
Please find at the page 110 the list of all the differences from TERA
TO OCTA.
SUMMARY
SECTION 1: WARNING ........................................................................................................................... 5

SECTION 2: SPECIFICATIONS .............................................................................................................. 7
1. TECHNICAL SPECIFICATIONS ........................................................................................................ 8
2. COMPUTER SPECIFICATIONS...................................................................................................... 10
SECTION 3: EXTERNAL DESCRIPTION .............................................................................................. 11
1. EXTERNAL VIEW ......................................................................................................................... 12
2. EXTERNAL HARDWARE DESCRIPTION ........................................................................................ 12
SECTION 4: INTERNAL DESCRIPTION ............................................................................................... 13
1- UNIT DESCRIPTION ..................................................................................................................... 14
1.1 MODULE ....................................................................................................................... 14
1.2 MODULE DESCRIPTION ................................................................................................. 15
1.3 MODULE POSITION ....................................................................................................... 16
2- ELECTRONICS ............................................................................................................................. 17
2.1 BLOCK DIAGRAM........................................................................................................... 17
2.2 EXPLODED VIEW ........................................................................................................... 18
SECTION 5: SOFTWARE ...................................................................................................................... 19
1. SOFTWARE MENU ARCHITECTURE ............................................................................................ 20
2. MENU ......................................................................................................................................... 21
2.1 MAIN MENU ................................................................................................................. 21
2.2 MAIN REAGENT COMPARTMENT ................................................................................. 22
2.3 SECONDARY REAGENT COMPARTMENT ....................................................................... 23
2.4 DISPOSABLES & WASTE ................................................................................................ 24
2.5 MAINTENANCE & CLEANING ........................................................................................ 25
2.6 PRIMING WINDOW (only when using service password) ............................................. 26
2.7 SHUTDOWN WINDOW (only when using service password) ........................................ 27
3- SETTINGS & ADVANCED SETTINGS............................................................................................. 28
3.1 TIME .............................................................................................................................. 29
3.2 NETWORK ..................................................................................................................... 30
3.3 CUSTOM ........................................................................................................................ 31
3.4 SCHEDULE ..................................................................................................................... 32
3.5 PARAMETERS ................................................................................................................ 33
3.6 TEST MODE ................................................................................................................... 35
3.6.1 Migration ....................................................................................................... 35
3.6.2 Fluidics ........................................................................................................... 36
3.6.3 Arm ................................................................................................................ 37
3.6.4 Loaders .......................................................................................................... 38
3.6.5 Racks .............................................................................................................. 39
3.6.6 Injection ......................................................................................................... 40
3.6.7 Conveyor ........................................................................................................ 41
3.6.8 Containers...................................................................................................... 42
3.7 FIRMWARE UPDATE ...................................................................................................... 43
3.7.1 Update the instrument firmware release ...................................................... 43
3.7.2 PSOC Boards Firmware .................................................................................. 45
3.8 LOG ............................................................................................................................... 46
3.9 OPTICAL ........................................................................................................................ 47
3.9.1 Acquisition ..................................................................................................... 47
3.9.2 Calibration ..................................................................................................... 47
3.9.3 Monochromator ............................................................................................ 48
3.10 LANGUAGE .................................................................................................................... 50
3.11 COMMAND ................................................................................................................... 51
SECTION 6: HOW TO ........................................................................................................................... 52
1. HOW TO SAVE THE CAPILLARIES ................................................................................................ 53
2. HOW TO ACTIVATE THE CAPILLARIES ........................................................................................ 54
3. HOW TO BACKUP THE PARAMETERS AND ERROR LOG FILE (LOG_IHM) ................................... 55
4. HOW TO SWITCH FROM LCD SCREEN TO VNC (COMPUTER SCREEN) AND USE VNC ................ 55
5. HOW TO VISUALIZE THE LOG FILE .............................................................................................. 55
6.1 INSTRUMENT LOG FILES ............................................................................................... 55
6.2 PHORESIS LOG FILES (shutdown cycle, migration conditions) ...................................... 56
SECTION 7: ADJUSTMENTS & CALIBRATIONS ................................................................................. 57

1. 6 & 8 WAY VALVES ..................................................................................................................... 58
1.1 MECHANICAL ADJUSMENTS ......................................................................................... 58
1.2 ROTATION ENCODER CALIBRATION .............................................................................. 60
2. DISTRIBUTOR & SEGMENT LOADER ........................................................................................... 61
2.1 MECHANICAL ADJUSTMENT OF THE DISTRIBUTOR TRAY ORIGIN (only if required) ..... 61
2.2 SEGMENT LOADER – MECHANICAL ADJUSTMENT........................................................ 64
2.3 SEGMENT LOADER – DETECTION CALIBRATION ........................................................... 65
3. INJECTION FORK ......................................................................................................................... 67
3.1 MECHANICAL ADJUSTMENT OF THE INJECTION FORK – SN < 149 (only if required) .... 67
3.2 MECHANICAL ADJUSTMENT OF THE INJECTION FORK – SN > 149 (only if required) .... 70
3.3 DRAINING POSITION ..................................................................................................... 71
3.4 FILLING POSITION ......................................................................................................... 74
3.5 DILUTION SEGMENT TRANSFER .................................................................................... 76
3.6 ANODE TANK UNDERNEATH CAPILLARIES (TERA) ........................................................ 77
3.7 ANODE TANK UNDERNEATH CAPILLARIES (OCTA) ........................................................ 78
3.8 DILUTION SEGMENT INJECTION (TERA) ........................................................................ 78
3.9 DILUTION SEGMENT INJECTION (OCTA) ....................................................................... 80
4. SYRINGE ..................................................................................................................................... 81
4.1 SYRINGE INSTALLATION ................................................................................................ 81
4.2 FORCE SENSOR CALIBRATION ....................................................................................... 82
4.3 FORCE SENSOR TEST ..................................................................................................... 82
5 RACK HOLDER ............................................................................................................................ 83
5.1 BELT .............................................................................................................................. 83
5.2 RACK LOADING.............................................................................................................. 83
5.3 RACK UNLOADING ........................................................................................................ 86
6 NEEDLE ARM .............................................................................................................................. 88
6.1 TUBE.............................................................................................................................. 88
6.2 WELL ............................................................................................................................. 90
6.3 DILUENT ........................................................................................................................ 91
6.4 CLEANING ..................................................................................................................... 92
6.5 ANTISERA TUBE ............................................................................................................. 92
6.6 IT PREDILUTION TANK ................................................................................................... 93
6.7 HBA1C CAPILLARY TUBES .............................................................................................. 93
7 BARCODE READER ...................................................................................................................... 95
7.1 CALIBRATION: RackTubeNReadingCalib.sh ................................................................... 95
7.2 DEFAULT SETTING ......................................................................................................... 96
7.3 MODIFY THE SETTING ................................................................................................... 97
7.4 INSTALLATION OF A NEW BARCODE READER ............................................................... 98
8 DCN ............................................................................................................................................ 99
9 CATHODE TANK ........................................................................................................................ 100
9.1 FILLING SENSOR (C24) ................................................................................................. 100
9.2 OVERFLOW SENSOR (C32) .......................................................................................... 100
SECTION 8: GLOSSARY..................................................................................................................... 101
SECTION 9: SCHEMATICS ................................................................................................................. 103
1- ELECTRONICS ........................................................................................................................... 105
1.1 CAPILLARYS 3 TERA (SN < 149) - CAN BUS & POWER SUPPLY..................................... 105
1.2 CAPILLARYS 3 TERA (SN < 149) - LAYOUT & WIRING .................................................. 106
1.3 CAPILLARYS 3 TERA (149 ≤ SN ≤ 220) - CAN BUS & POWER SUPPLY ........................... 107
1.4 CAPILLARYS 3 TERA (149 ≤ SN ≤ 220) - LAYOUT & WIRING ......................................... 108
1.5 CAPILLARYS 3 TERA (221 ≤ SN ≤ 518) - CAN BUS & POWER SUPPLY ........................... 109
1.6 CAPILLARYS 3 TERA (221 ≤ SN ≤ 518) - LAYOUT & WIRING ......................................... 110
1.7 CAPILLARYS 3 TERA (SN ≥ 519) - CAN BUS & POWER SUPPLY..................................... 111
1.8 CAPILLARYS 3 TERA (SN ≥ 519) - LAYOUT & WIRING .................................................. 112
1.9 CAPILLARYS 3 OCTA - CAN BUS & POWER SUPPLY ..................................................... 113
1.10 CAPILLARYS 3 OCTA - LAYOUT & WIRING ................................................................... 114
2- FLUIDICS ................................................................................................................................... 115
SECTION 10: DIFFERENCES FROM TERA TO OCTA ..................................................................... 116
SECTION 1: WARNING
SECTION 1: WARNING AND SAFETY NOTES
Follow all warnings and instructions marked on or supplied with the instrument. This service manual includes all information
and warnings necessary for reliable and safe operation and maintenance. If the instrument is used in any other way than that
specified by the manufacturer, operator’s safety may be compromised.
The instrument has been designed to be used indoors.
Do not use the instrument near water or in wet locations.
Do not place the instrument on an unstable cart, stand or table. Otherwise personal injury or damage to the instrument
might occur.
This instrument should be operated from the type of power source indicated on the instrument’s label and in this instruction
manual. Before switching the instrument on, check that the voltage setting corresponds to the main power source. If you are
unsure of the type of power available, consult your local power company.
The instrument is equipped with a 3-wire grounding-type plug. The plug should only be connected to a grounded power
outlet. Never use a ground adapter plug to connect the instrument to a socket lacking a ground connection terminal.
Never interrupt ground leads or disconnect the ground terminal inside or outside the instrument.
Use only fuses with appropriate rating value and of specified type. Make sure that the total amperage of all appliances
plugged into the power outlet does not exceed the outlet protection.
If an extension cord is used with this instrument, ensure that the total instrument amperage rating does not exceed the
extension cord power rating.
Disconnect the instrument from any power source before opening for testing, cleaning, adjustments, servicing or
maintenance.
Do not remove any covers when the instrument is connected to the main power source as this may expose dangerous live
terminals.
Never defeat interlock switches. They are provided to prevent access to unsafe areas.
Any adjustment, maintenance and servicing of an uncovered instrument without disconnecting it from power outlet must
only be made by qualified and trained personnel, aware of the risks involved.
If it is believed that the instrument has been damaged and is unsafe, avoid using it and contact Sebia’s authorized
representative.
Manufacturer's Responsibility
Sebia or Sebia’s authorized representative are responsible only for defects in material and services if:
• Adjustments, alterations or servicing have been made by authorized personnel.

• Electricity supply is in accordance with instrument specifications.
• Instrument is used according to instructions.
The manufacturer is not responsible for damage from misuse, abuse, accident and disasters, improper return shipping, or
adjustments, alterations and servicing done by unauthorized personnel.
The characteristics of CAPILLARYS 3 are described in this manual. SEBIA has the right to modify them without prior notice.
SECTION 2: SPECIFICATIONS
SECTION 2: SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS
Migration Liquid-flow
: capillary electrophoresis in 12 silica capillary
tubes, migrations takes place in fully controlled temperature
conditions using a Peltier device.
Detection Deuterium
: lamp, LED, optical grating, C.MOS diode array
detector, optical fibers.
Loading Primary
: maximal capacity of 180 tubes (i.e. 15 sample
racks), uninterrupted throughput on sample racks (12
positions available).
Up to 30 reagent cups can be managed by an automatic
loading system.
Identification By
: RF-ID tags on the sample racks, on primary sample
tubes, on control samples and calibrators tubes according to
the procedure.
Sampling All
: techniques except HEMOGLOBIN(E) and HbA1c:
(see the document Samples are taken directly from uncapped tubes (without
"Characteristics of tubes cap, 13 to 16 mm in diameter, 75 to 100 mm high) or from
to use according to the 1.5 mL micro-tubes positioned on empty primary sample
instrument" for additional tubes with 13 mm diameter used as holders, sample
information) volume: 20 to 40 µL.
HEMOGLOBIN(E) and HbA1c techniques:
Samples are taken after homogenization directly in capped
tubes (from 11 mm to 13 mm in diameter, caps with 17 mm
maximum diameter and capped tubes with 90 mm maximum
high), aspiration of calibrators and blood controls in capped
conical tubes placed on adapters, minimal volume of sample
in primary tubes: 1 mL, sample volume for analysis: 20 µL.
Data processing by the - 2,400 patients per analysis program,
PHORESIS software - :control and correction,
- statistical calculations generated by analysis program,
- abnormal values detected,
- curves traced in graph format, data printouts,
- unidentified curves highlighted,
- immunofixation images and quantitative specific protein
results imported and printed,
SQL client / server database: unlimited storage capacity of
patients database, networking capabilities for multi-clients
management,
- curve search and edit.
Display touch
: screen user interface,
Output connections CAPILLARYS
: 3 AUTOMATED INSTRUMENT:
- USB port (not used),
- Ethernet port1 for connection to PC (and PHORESIS
software),
- serial port,
- SD card (not used)
HARDWARE MODULE:
- Connections to analyzer, printer, keyboard, mouse,
modem, network, bar code reader, scanner.
Dimensions L.: 90 cm x H. 54 cm x D. 67 cm
Weight 75
: kg
Installation category 2:
Ingress protection IP
: 20
Sound pressure level <: 60 dB(A)
Power 350
: VA
Power supply 100
: - 240 V, 50/60 Hz
Heat : BTU2
750
1 Not intended for direct connection to telecommunication networks

2 BTU
(British Thermal Unit) = 1055 J
2. COMPUTER SPECIFICATIONS
PC MINIMUM REQUIREMENTS
• 3.2 GHz Intel® Core i5 processor, Windows® 7 (32 or 64 bits)
• 23-inch SVGA screen with a definition of 1920 x 1080
• Memory RAM 4.0 GB
• Graphic card 65536 colors
• Ports: USB (printer, keyboard, mouse, barcode reader, scanner), RJ45

(Ethernet), COM (RS232 to analyzer for troubleshooting)
• CD/DVD player
• Keyboard, mouse
• Two Ethernet (network) cards
SECTION 3: EXTERNAL DESCRIPTION
SECTION 3: EXTERNAL DESCRIPTION
1. EXTERNAL VIEW
Figure 1: Front view

1
3
4
2. EXTERNAL HARDWARE DESCRIPTION
It consists of:
- On the front panel (see figure 1):
• 1 - touch screen,
• 2 - conveyor lids (left & right),
• 3 - reagent lid
• 4 - reagent cup (dilution segment) compartment
- On the right panel (see figure 1):
• 5 - micro-plate compartment door.
- On the rear panel:
• 6 - ON/OFF switch
• 7 - A.C. power source inlet with fuse compartment,
• 8 - Ethernet port,
• 9 - COM port,
• 10 - SD card port (not used).
SECTION 4: INTERNAL DESCRIPTION
SECTION 4: INTERNAL DESCRIPTION
1- UNIT DESCRIPTION
1.1 MODULE
It consists of:
- On the left, reagent lid opened:
• Primary reagent compartment.
- In the middle, reagent lid opened:
• Needle arm,
• Rack holder,
• Fluidics metal panel,
• Secondary reagent compartment.
- On the right, reagent lid opened:
• Distributor tray,
• Injection fork,
• Migration assembly,
• Optics.
- At the back, rear cover removed:
• Fluidics.
1.2 MODULE DESCRIPTION
- Needle arm
• 4x motors
• 3x translation encoders
• 1x barcode reader rotation origin opt sensor
• 1x barcode reader
• 1x flex piercing needle (sample probe)
- Conveyor
• 1x motor
• 3x opto sensors (the first one on the right, single rack introduction is not used)
• 4x rack stoppers (electromagnets)
• 1x RFID PCB
- Rack holder
• 3x motors
• 1x rotation origin opto sensor
- Distributor tray
• 1x motor
• 1x rotation encoder
- Injection fork
• 2x motors (3x if S/N < 150)
• 0x rotation encoder (1x if S/N < 150)
- Migration
• 12x capillaries
• 1x Peltier assembly
- Optics
• 13x emission optical fibers
• 13x reception optical fibers
• 1x blue LED (used at 420nm)
• 1x deuterium lamp (used at 200nm)
• 1x monochromator
• 1x motor
• 1x rotation encoder
• 2x electromagnets
- Fluidics
• 3x motors
• 2x rotation encoders
• 1x translation encoder
• 1x force sensor
• 1x 8-way valve
• 1x 6-way valve
• 1x syringe
• 1x liquid pump (P2)
• 1x air pump (P1)
- Reagents
• 1x waste container
• 1x wash solution container
• 1x H2O container
• 4x flexible reagent container positions: buffer, H2O, hemolyzing solution…
• 3x cleaning solution tube positions
• 4x secondary reagent vial positions (3 cooled and 1 at ambient temperature)
• 1x IT antisera cartridge (cooled compartment)
• 1x Peltier assembly
- Loader
• 2x motors
• 4x sensors (2 origin opto sensors, and 2 sensors to detect the presence of segments and micro plates)
1.3 MODULE POSITION
2- ELECTRONICS
2.1 BLOCK DIAGRAM
2.2 EXPLODED VIEW
SECTION 5: SOFTWARE
MAIN MENU
START LID SETTINGS SHUTDOWN

OPERATOR & ICONS – CURRENT TECHNIQUE MAIN REAGENT SECUNDARY REAGENT DISPOSABLE MAINTENANCE
INSTRUMENT WORKING DATE – & CHANGE OF COMPARTMENT COMPARTMENT & WASTE & CLEANING
NAME PHORESIS CONNECTION TECHNIQUE
SETTINGS ADVANCED
SETTINGS
SECTION 5: SOFTWARE
PARAMETERS TEST UPDATE LOG OPTICAL MINICYCLE LANGUAGE SCRIPT TOUCHPAD DCN BARCODES COMMAND TESTS
TIME MODE
Capillarys 3 TERA & OCTA – Service Manual

NETWORK
MOTHERBOARD CALIBRATION MECHANICAL SETTINGS
1. SOFTWARE MENU ARCHITECTURE
CUSTOM MIGRATION ACQUISITION MOTORS

BOARDS STATUS FLUIDICS
FLUIDICS MONOCHROMATOR POWERS
UPGRADE BOARDS
SCHEDULE RACKS
ARM NEEDLE
DISTRIBUTION
CUPS
LOADERS RACKS
LOG_IHM DISTRIBUTOR INJECTION
RACKS
Page 20 of 120
LOG_AUTOMATE
INJECTION MICRO-PLATES NEEDLE - BARCODE READER
COUNT _ANALYSE ACKS
CONVEYOR INJECTION
IMG_PANEL
CONTAINERS PRESSURE & VACUUM
FLUIDIC NEEDLE
FLUIDIC SYRINGE
FLUIDIC CATHODE
FLUIDIC ANODE
DCN
BARCODE READER
DILUTIONS
v1.15 P1 – Date: March 18th, 2019

2. MENU
2.1 MAIN MENU
First menu displayed when turning ON the Capillarys 3.
Date
User’s name and
instrument’s name
Instrument status
Displays main reagent

compartment
information
Displays secondary
reagent compartment
information
Displays disposables
& waste information
Displays maintenance
& cleaning information
Cycle’s name and status
With service
With service
password: opens the
password: opens the
priming dialog
shutdown dialog
window
window
Without service
Without service
password: starts the
password: starts the
priming
shutdown
Opens the lid Displays the settings
2.2 MAIN REAGENT COMPARTMENT
Lot number
Expiration date
Level in %
Name
To validate and return

to the main menu
The colour of the container depends of the type of reagent inside compartment (given by the RF-ID tags).
Only the reagents used according to the selected technique/in progress are back lighted.
When one container is empty or the expiration date reached, the container icon on the main menu will be coloured in orange
or red instead of green and the container into Capillarys 3 will be flashing.
Note: Since the version 1.06, pressing “+” or “-“ for more than 3 seconds will set the container to 100% or 0% directly.
2.3 SECONDARY REAGENT COMPARTMENT
Lot number
Expiration date
Level in %
Name
Position
CDT
To validate and return

to the main menu
2.4 DISPOSABLES & WASTE
Click on the icons to reset the individual counters or waste container level.
The dilution segment waste counter can be reset automatically when taking out the dilution segment bin for more than 10s.
The waste container level can be reset from the main compartment window.
2.5 MAINTENANCE & CLEANING
The last maintenance will be the date of the latest service maintenance.
This service maintenance must be done at least once a year (preventive maintenance).
Click on the name of the cleaning cycle to be performed.

The cycles “decontamination” and “Capiclean” must be done at least once a week.
Each of these two cycles takes approximately 11 minutes.
Note: For the moment, the indicators stay greyed whatever if the cycle is done or not.
2.6 PRIMING WINDOW (only when using service password)
1. Normal priming (regular daily priming or once the shutdown cycle has been performed)
2. Fast priming (once the regular priming has already been done)
Note: Since the version 1.06, the secondary reagents (IT diluent, antisera, CDT diluent or Neonat diluent) are no longer
required to perform a priming.
2.7 SHUTDOWN WINDOW (only when using service password)
1. Normal shutdown (regular daily priming)
2. Rinsing shutdown (normal shutdown + rinsing fluidics with water)

Required: 6x reagent containers filled with water
3. Transport shutdown (normal shutdown + rinsing fluidics with water + draining of the fluidics)
Required: 6x reagent containers filled with water, then 6x empty reagent containers
Note: Since the version 1.06, the buffer, hemolyzing solution and secondary reagents (IT diluent, antisera, CDT diluent or
Neonat diluent) are no longer required to perform a shutdown.
3- SETTINGS & ADVANCED SETTINGS
From the main menu, select “Settings”, click on the password box and enter “4644”.
Note that it takes few seconds to get the display of the advanced settings.
This dialog window is divided in two parts: “Settings” and “Advanced Settings”.
The first one is displayed every time and the second one only when using the service password.
In the next pages, description of the settings from the left hand top corner to the right bottom corner.
3.1 TIME
When installing Capillarys 3, check the date and time.
If the time does not change at all, check the soldering of the quartz on the motherboard.
If the time does not change when the instrument is OFF, check the soldering of the capacitor on the motherboard.
Note: since the version 1.15, the date and time are synchronized by Phoresis (when the connection is ONLINE).
Quartz
Capacitor
3.2 NETWORK
Click on “DHCP” to switch from dynamic to static IP address.

The dynamic IP address is active when the check icon is shown, be careful to select this option only when the Capillarys 3 is
connected to a DHCP server.
If no Ethernet cable is plugged when powering ON the instrument, the setup will be set to static IP address (192.168.0.200 by
default).
Set-up the connection on Phoresis (available only when Phoresis is OFFLINE):
List of the ports used on the PC and the instrument (must be opened):
Port 21: used for the ftp link Port 22: used for the SSH link
Port 80: used for the http link Port 4532: instrument
Port 5000: used for the connection Phoresis / Instrument
Port 5432: used for the connection Phoresis / PostgreSQL
Port 5900: used for the VNC connection
3.3 CUSTOM
This menu allows the customer to:
- customize some warning levels,

- activate or deactivate a capillary,
- enable or disable the alarm when the lid must be opened (reagent containers will still be flashing in green)
- set the waiting time before to inject a segment which is not full.
3.4 SCHEDULE
Capillarys 3 is able to perform priming, shutdown, Capiclean and bleach cycles automatically.
Select the analysis programs, days and time for each working day Capillarys 3 should start automatically.
Same principle for the automatic shutdown and cleaning cycles.
Note: if during the idle mode, the instrument is awake by touching the screen, it will not start automatically after.
In that case, the priming must be started manually.
3.5 PARAMETERS
Select “Parameters” from the “Advanced settings” menu. Search by number

Search by name
Search by criteria
Modification zone: Enter the value

and click on “Valid” icon.
Modify: click to change the value of

the parameter for the selected
technique
Customize: click to change the value

of the parameter for all the
techniques
Replace: to replace the default value

Add: to add entered value to the default value
Subtract: to subtract the entered value to the default value
Do nothing: to delete the customized value (default value will be applied)
After modifying the value of the parameter, click on “Close Edition Customized” and click on the “Exit” button.
Note: If the “Close Edition Customized” button is not clicked, the value of the parameter will be taken into account only after
the next priming.
Select “Yes” with the check icon to the question “Do you want to save this parameter?”
3.6 TEST MODE
3.6.1 Migration
Upper part of the test mode is dedicated to the High Voltage:

It allows to test the High Voltage, by setting the voltage and current then switch ON/OFF the High Voltage.
It also displays the voltage and current values.
Note: When testing the HV, always set the current to maximum 1000µA.
Second part is dedicated to the capillaries temperature:

Set the requested temperature in °C (from 30 to 45°C) and switch ON/OFF the regulation.
Press “View Regulation Curve” to see the temperature (red) in real time and the regulation curve (blue).
Note: 45°C must be reached in 60 seconds (from ambient temperature)
The bottom part is dedicated to the light sources used during migration (deuterium lamp and 420nm blue LED).
It also allows to switch ON/OFF the capillaries fan, UV filter and shutter.
3.6.2 Fluidics
Full: Empty:
Empty: Full:
Empty: Full:
In the “fluidics” tab, we can switch ON/OFF the twelve electro-valves and the two pumps.
The value of the pressure and vacuum sensors are displayed in the bottom of the page.
Note: the pressure offset must be within 800 and 1300 mBars, and vacuum offset must be within 0 and 5 mBars.
The right area concern the sensors status and the motors. By selecting one of the two tabs, it will switch between these two
windows.
In the motor window, select the motor you want to drive (M15, M16 or M17).
Depending of the selected motor, the arrows may change to indicate the direction of the movement.
The origin of the syringe is the lower position (full) and the force sensor zero volume position is the upper position (empty).
In the sensors window, you can see the status of the end waste, syringe, pump 2 and over flow sensors.
3.6.3 Arm
- Origin: 8000
- Not in origin: 14000
In this tab, there are four parts. Each part matches with a motor and its encoder.
To command a motor, four buttons are used:
• The one on the left is to move a motor to its origin.

• The second and third ones are to move a motor to the selected direction.
• The last one is to stop the movement immediately.
Note: If the needle is down, the needle arm cannot move (arrows are greyed).
Move the needle to the home position or press “allow X, Y movements” to be able to move the needle arm.
The status of the DCN is displayed on the bottom of this tab.
Note: this is a raw value, it is not the same from one instrument to another one.
This is only to check that the signal changes when touching the needle.
3.6.4 Loaders
- Origin: 8000
- Detected: 14000
- Not detected: 8500
- Origin: 8000
- Detected: 14000
- Not detected: 9000
3.6.5 Racks
- Origin, close to 7000.

- Not in origin, close to 12000.
3.6.6 Injection
Note: If the injection fork is up, it cannot move forward/backward (arrows on Y axis are greyed).
Move the injection fork down or press “allow Y Inj move” to be able to move injection fork.
3.6.7 Conveyor
- No rack detected, close to 7000.

- Rack detected, close to 12000.
- No rack RF-ID tag detected.
Under you can see status of the three opto sensors of the conveyor and the ID of the rack which is read by RF-ID technology.
At the bottom of this tab, you can switch ON/OFF the four rack blockers of the conveyor.
3.6.8 Containers
Green: RF-ID tag detected, its ID

number is displayed
Red: RF-ID reader failure: Blue: no RF-ID tag detected

check the cable and the RF-ID
board
At the top of this tab, you can see the ID of the six main reagent containers, the waste container and the status of the waste
container sensor.
In the middle you have ID of the secondary reagent containers (diluents and IT tubes).
Underneath, you can light up containers with different colours.
At the bottom you can control the temperature of the secondary reagents compartment,
Press “View Regulation Curve” to see the regulation curve in real time (temperature in red and regulation curve in blue).
3.7 FIRMWARE UPDATE
The first tab “Motherboard” includes the date and the version of the firmware.
The second tab “Board Status” allows us to see the version of the firmware inside each PCB and the version of its boot.
The last tab “Upgrade Boards” is used to upgrade or reload the firmware of the boards.
3.7.1 Update the instrument firmware release
Important: make sure that the shutdown cycle has been performed. If so switch ON the unit and reach the main menu.
Do not perform the priming cycle. Do not perform the firmware update from the Ready mode.
Reach the folder C:\Program Files\Phoresis\Capillarys\Firmware_4

Execute the batch file Upgrade.bat
If the “TFTPD 32” is not installed there will be a message displayed. If so, then, install it.
Note:
The installation file of “TFTPD 32” is located into C:\Program File\Phoresis\Capillarys\Firmware_4.
Once installed, “TFTPD 32” is located into C:\Program Files\Tftpd32
Important: start up “TFTPD 32” to make sure it’s correctly installed and then close it.
(“TFTPD 32” must be closed before to start the script of the firmware upgrade)
The following windows will be displayed:
3 Instrument IP address or Host Name
2
1
In the software “Kitty”, click on SSH_CAPI3 and then click on Load.

The Host Name, displayed will be “EC3-0”.
− If the instrument is using dynamic IP address, fill the Host Name with the serial number of the instrument (4 digits
are needed, example: ec3-0027 for the serial number 27).
− If the instrument is using permanent IP address, replace ec3-0 by the IP address of the Capillarys 3 instrument.
Click on “Open”.
For the very first connection between

the computer and the instrument,
a security alert is displayed.
Click on “Oui” (Yes) to validate.
The upgrade begin automatically, it ends when the instrument restart (approximately 15 minutes)
The connection is then lost and the following error message is displayed. Click on “OK” and the upgrade is done.
3.7.2 PSOC Boards Firmware
The Capillarys 3 consists of 10 motor boards, 3 Power Boards, and 1 Container Board.
In total, 14 PSOC Boards are connected on the CAN Bus.
During the start up the correct communication of all the 14 PSOC Boards is verified.
Therefore, each PSOC Board is numbered, but its number is not always the same:
• During the firmware loading and during the step “Numbering done”, the first board detected is the nearest board from the
motherboard (the monochromator motor board) :
Number PSOC Board Type Designation

1 Motor Board Monochromator
2 Motor Board Segment Loader
3 Power Board Migration PCB
4 Motor Board Distributor
5 Motor Board Rack Y & Injection Y
6 Motor Board Rack Holder
7 Motor Board Injection Z
8 Power Board Conveyor PCB
9 Container Board Container A
10 Power Board Fluidic PCB
11 Motor Board Valves
12 Motor Board Arm X
13 Motor Board Arm Y
14 Motor Board Arm Z
• Once the step “Numbering done” is performed, the boards must be configured. During this set up step, it’s not possible to
communicate with the next board. Therefore, the numbering is “reversed” : the board number one is now the motor board
Arm Z (the farthest from the mother board):
Number PSOC Board Type Designation

1 Motor Board Arm Z
2 Motor Board Arm Y
3 Motor Board Arm X
4 Motor Board Valves
5 Power PCB Fluidic PCB
6 Container Board Container A
7 Power PCB Conveyor PCB
8 Motor Board Injection Z
9 Motor Board Rack Holder
10 Motor Board Rack Y & Injection Y
11 Motor Board Distributor
12 Power Board Migration PCB
13 Motor Board Segment Loader
14 Motor Board Monochromator
Note: the firmware release of the motor board is visible Instrument Firmware 1.15 P1
from the main menu, select successively:
Motor Board Firmware 0.82
“Settings”
Power Board Firmware 0.51
→ (Enter the service password 4644) Update
Container Board Firmware 0.28
→ Select the tab “Board Status”
DCN Board Firmware 0.3
3.8 LOG
This allows you to visualize all the error messages that appeared on the instrument and when it appears.
It allows also visualizing the latest lines of the logfile that happened before the latest error (“log_automate”) or to see the
number of migrations cycles ran on the instrument (“count_analyse”).
Using the script Backup Parameters, available in the S.K.B, it’s now possible to save the Log Files in the PC.
3.9 OPTICAL
3.9.1 Acquisition
From the “ready” mode, select “Advanced settings”, “Optical” and “Acquisition”. Click on “Shutter OFF” and “Filter OFF” and
then click on the blue arrow to start the acquisition. Click on the blue square to stop the acquisition before to leave this window.
Capillary 12 channel
Capillary 1 channel
Reference channel
If the acquisition is not good, dismantle the capillaries and the optical fibers to clean them.
The cleaning of the optical fibers and the capillary detection windows is the same as on Capillarys 2/Capillarys 2 Flex Piercing.
3.9.2 Calibration
As for the acquisition and monochromator, you must be in “ready” mode

before to start the optical calibration.
Select the tab “Calibration” and press on the blue arrow to start the
calibration.
In HbA1c technique,
this is the LED’s
In Protein 6/Immunotyping/CDT, the integration time must be below
current
20ms.
In Hb/HbA1c, the integration time must be below 10ms and the LED
current must be below or equal to 900.
The thirteen optical levels must be as aligned as possible.

The reference channel (on the left) should be at the same level or above
the other capillary channels.
It is normal to observe as a triangle shape (yellow dashed lines).

It is due to the monochromator production method.
Reference channel Capillary 1 channel Capillary 12 channel

3.9.3 Monochromator
Important:
To perform the scan, the deuterium lamp and the 420nm blue LED are used, whatever the technique.
Do not perform the scan if one of the two light sources is not working.
As for the acquisition, the unit must be in “ready” mode.
Press on « Scan ». The scan is made in 2 steps:

- First scan movement for 656 nm peak detection.
- Second, complete scan for all wavelengths.
Wait until the end of the scan.
Correct scan of the monochromator:

• -3 < “mono error” < +3
• position of the blue and green lines
• correct general shape (see example below)
The green line indicates the selected wavelength.

The white/blue line indicates the current wave length position.
Note: If you do not see the white line, it means that it is displayed underneath the green line.
So the current wavelength is the selected one.
Click on “Nm”, the monochromator will move to the selected wavelength which is 200nm by default.
Note: default wavelength is 200nm for Protein 6 / Immunotyping / CDT.

Default wavelength is 420nm for Hb / HbA1c.
Examples of bad scan of the monochromator:
• If the detection zone is bad then it will generate a value “mono error” that is out of the range :
The purple line (detection zone) is shifted to The purple line (detection zone) is shifted to
the left side → increase P2840 and renew the the right side → decrease P2840 and renew
scan of the monochromator. the scan of the monochromator.
• The UV filter (top side of the monochromator assembly) is blocked during the scan:
3.10 LANGUAGE
When changing the language, the instrument needs to be restarted.
3.11 COMMAND
Direction
Stop Home Position Start
“Powers” tab:
- select the electromagnet, electrovalve, pump or fan
- turn it ON or OFF
“Motors” tab:
- select the motor
- press on “Hold Current”
- select the direction “Left”, “Right”, “Up”, Down”, “Front” or “Back”
- enter a number of “Steps”
- press on “Start” or “Home Position”
Note: the direction must be set correctly to go to the home position (otherwise the motor will go on the opposite direction)
Note: to reach the home position, the number of steps must be high enough (otherwise the motor will stop before to reach
the home position)
Note: in case of error, blockage… use the “Stop” to stops the motor immediately
SECTION 6: HOW TO
SECTION 6: HOW TO...
1. HOW TO SAVE THE CAPILLARIES
Turns ON the Capillarys 3, press on Settings and on MiniCycle.
Select Fluidic Cathode, tick the boxes Drain cathode tank, Filling cathode tank (select Water - 0µl - overflow), Pressure in
cathode tank (select 4000mBars) and press on Start.
Wait 5 minutes, perform the cycles Drain cathode tank and Pressure in cathode tank with 4000mBars and switch OFF the
instrument.
If possible: remove the liquid drop at the extremity of the capillaries with a cotton swab.
2. HOW TO ACTIVATE THE CAPILLARIES
Turns ON the Capillarys 3, reach the ready mode, press on Settings and select MiniCycle
Select Fluidic Cathode, tick the boxes Drain cathode tank, Filling cathode tank (select Wash - 0µl - No Overflow), Pressure in
cathode tank (select 4000mBars) and press on Start.
Select Test Mode - Migration, set a target at 45°C and press on OFF to turn ON the regulation.
Wait 8 minutes, set a target at 3000V and 1000µA and press on OFF to turn ON the High Voltage.
Wait 3 minutes, leave the Test Mode and perform the normal priming.
3. HOW TO BACKUP THE PARAMETERS AND ERROR LOG FILE (LOG_IHM)
Use the script “How to backup and restore the parameters” available in the Sebia Service Knowledge Base
4. HOW TO SWITCH FROM LCD SCREEN TO VNC (COMPUTER SCREEN) AND USE VNC
Use the script “Swap the screen LCD/PC” available in the Sebia Service Knowledge Base
The instrument must be set with the screen on VNC (see previous chapter: how
to switch from LCD screen to VNC).
Start UltraVNC Viewer (available in the Sebia Service Knowledge Base), no

installation required.
Enter the IP address or Host name and click on “Connect”.
The port should be opened in the Capillarys 3 before to be able to start the
connection.
Note: reagents containers are lighted few seconds before the port is opened.
5. HOW TO VISUALIZE THE LOG FILE
6.1 INSTRUMENT LOG FILES
Note: the instrument must be ON in order to visualize the Log File.
The instrument errors Log Files are available on the PC, using an Internet browser (Internet Explorer, Firefox, ...etc).
Enter : http://Instrument_IP into the search bar or into the Windows start icon.
For example :
A Log File scan be visualized in the instrument display :

From the main menu, select “Settings”, enter the service password (4644), reach the menu “Logs”.
Select the Log file to be visualized.
The Log Files can also be saved in the PC using the script “Backup Parameters” available in the Sebia Service Knowledge Base
6.2 PHORESIS LOG FILES (shutdown cycle, migration conditions)
Shutdown cycle :
Phoresis must be connected to the unit until the end of the shutdown in order to collect and update the Log Files.
Phoresis Log files are located into C:\Program Files\Phoresis\Capillarys\Capillarys3Driver\LogsYYYY-MM

It’s the files “Ec3Driver_DDD.log” (they can be opened with NotePad++).
Value = 7
Look at the indication {"Name":"ETATEXTINCTION","Value":"XX"} → beginning of the
shutdown cycle.
Value = 19
→ shutdown cycle
performed until the end.
Migration conditions :
The migration conditions are located into C:\Program Files\Phoresis\Capillarys\Capillarys3Driver\FtpProcessedFolder.
Folder YYYY-MM-DD. It’s the files “Unit SN_analysisDateNNN_NN_NNYYYY.xml”.
Saved information are :

Serial number of the unit Working date Current High Voltage
Reagents lot numbers Migration time Default High Voltage
Letter code program Number of dots on the curve Dots on each channel
Technique number Status of the High Voltage correction Barcode informations
Expected values :
Protein 6 HbA1c Immunotyping 6
Letter code program J @ F
Technique number 8 19 4
Status of the H.V correction 1 0 0
Current High Voltage 8 000 ± 5% 9 400 ± 5% 8 000
Default High Voltage 8 000 9 400 8 000
SECTION 7: ADJUSTMENTS & CALIBRATIONS
SECTION 7: ADJUSTMENTS & CALIBRATIONS
1. 6 & 8 WAY VALVES
1.1 MECHANICAL ADJUSMENTS
It consists of calibrating the origin of the 6 & 8 way valves.

Their respective hole must be aligned in front of the black coupling for the 6 way valve and the grey coupling for the 8 way
valve.
Note: You can use a mirror to see the hole of the valve instead of removing the fluidics metal plate.
Remove the secondary reagent compartment cover (4 screws highlighted in red on the picture below), unscrew the 4 locking
screws of the transparent cover (highlighted in yellow) and remove this cover.
Note: Be careful when putting back the secondary reagent compartment cover, it is easy to smash few tubing against the
fluidics metal plate.
Remove the thermal Styrofoam block, unscrew the 4 locking screws of the fluidic board and pull out the fluidic metal plate
for an easier calibration.
Select “Script” from the “Advanced settings” and “Fluidic”.
Unscrew the two couplings as shown on the picture below.
6-WAY VALVE 8-WAY VALVE

Tank valve Syringe valve
The two holes must be concentric.

Note: Use a flashlight to see them easily.
6-way valve adjustment (Tank valve)
If a container is plugged on position flex 2, remove it before to start the calibration, otherwise it will leak.
Select “EcartOriginCanal1VanneC” (Tank valve adjustment) and adjust the rotation of the valve with the arrows.
When the adjustment is correct press “Next” to save the calibration.
The number of motor steps can be

adjusted by selecting:
- the turtle (little movement)
- the rabbit (larger movement).
Exit icon
8-way valve adjustment (Syringe valve)

Select “EcartOriginCanal1VanneS” (Syringe valve adjustment) and adjust the rotation of the valve with the arrows.
When the adjustment is correct, press “Next” to save the calibration and then “Exit”.
1.2 ROTATION ENCODER CALIBRATION
This calibration must be done every time replacing a valve, its encoder or after doing the mechanical adjustment.
Reach the “Settings”, and select “MiniCycle” in order to renew the encoder calibration of the 6 and 8-way valves:
2. DISTRIBUTOR & SEGMENT LOADER
2.1 MECHANICAL ADJUSTMENT OF THE DISTRIBUTOR TRAY ORIGIN (only if required)
This calibration consists of calibrating the height and the origin position of the distributor tray.
Remove the screw highlighted in red below and the white flat ribbon cable holder shown by the red arrow.
Remove the separator metal plate located between the rack holder assembly and the distributor tray.
Remove the screw and the distributor cover:
Note: If the standby current is disabled, no need to follow both screenshots below.
Select "Loaders" from the “Test Mode” and rotate it to disable the standby current:
Loosen the distributor tray locking screws.
distributor tray
Place the calibration tool on the chassis below the distributor and then slide it frontward.
Move up and rotate the distributor tray so it is placed above the calibration tool and against its pin:
Select "Distributor" from the “MiniCycle”, ticks the box “Distributor to origin” and click on “Start”.
This MiniCycle consists of moving the Distributor motor to its origin.
When the rotation movement is done, the box is automatically unticked and then press “Exit”.
Tighten the two locking screws of the distributor tray and remove the calibration tool.
Note: When tightening the locking screws, hold all the parts together to minimize the mechanical gap.
2.2 SEGMENT LOADER – MECHANICAL ADJUSTMENT
The first step of this adjustment consists of powering ON the electromagnet of the segment loader and adjusting its position.
You must not see the two segment loader clips anymore.
OFF → segment loader clips are visible ON → segment loader clips must not be visible anymore
2.3 SEGMENT LOADER – DETECTION CALIBRATION
From the “MiniCycle”, reach the origin position the injection fork, the distributor tray and the segment loader.
From the “Test Mode”, reach the tab “Loaders” and move down the segment loader until it is totally down, hitting the
segment detection sensor (press the down arrow several times).
Note: all values below must be defined with the segment loader down.
• Detection of the segment on the distributor tray : P1646
Allow the detection of the segment on the distributor after a loading and check that the segment has been well ejected after
an unloading.
The first value “S1” to get is the detection level for a segment placed on the distributor tray.
Place by hand a segment on the distributor tray and use the “MiniCycle” - “Distributor” to rotate the distributor in the
segment loading position (tick the box “Rotate distributor to”, select “Segment loading” and “first”).
In test mode, get the value of the detection level with the segment placed on the distributor tray.
The second value “S2” to get is the detection level with some segments inside the loader.
In the “MiniCycle”, reach the origin of the distributor (first choice).

Place 10 segments in the loader and get the value of the sensor from test mode.
• Detection of the segment on the loader : P1647
The third value “S3” is the detection level with a segment in the loader.
Let only one segment in the loader and get the value of the sensor.
The fourth value “S4” is the detection level without any segment in the loader.
Remove the segment in the loader and get the value of the sensor.
From the “MiniCycle” – “Distributor”, reach the home position of the segment loader (third choice).
P1646 = [(S1+S2)/2] -100

Reach the parameters and customize both parameters with the calculated values
P1647 = (S3+S4)/2
3. INJECTION FORK
3.1 MECHANICAL ADJUSTMENT OF THE INJECTION FORK – SN < 149 (only if required)
This calibration consists of calibrating the height and the origin position of the injection fork – SN < 149.
Please refer to the previous calibration to see how to remove separator metal plate located between the rack holder
assembly and the Distributor tray.
Note: If the standby current is disable, no need to follow the three screenshots below.
Select "Injec." from the “Test Mode” and click on a direction for each motor to disable the standby current (verify that you can
move the injection fork without risk before clicking on a direction).
Move manually the injection fork to have a good access to the locking screws and unscrew them.
Injection fork
Select "Distributor" from the “MiniCycle”, ticks the box “Distributor to origin” and press “Start”.
This MiniCycle consists of moving the Distributor motor to its origin.
When the rotation movement is done, the box is automatically unticked. Then press “Exit”.
Select "Injection NewTank" from the “MiniCycle”, ticks the box “Injection fork to origin” and click on “Start”.
This MiniCycle consists of moving the Injection fork to its origin.
When the MiniCycle is done, the box is automatically unticked, and then press “Exit”.
Disable the standby current of the Injection elevator and translator motors (M9 and then M8 only!)
Select “Injec.” from the “Mode Test” and click only on indicated directions.
Place back the Injection fork with the calibration tools and adjust manually its position to be in contact with the distributor
tray as shown on the picture below.
Tighten the two locking screws of the distributor tray and the injection fork.
Note: When tightening the locking screws, hold all the parts together to minimize the mechanical gap.
Then move manually and carefully the Injection fork (Y and Z direction) to be able to remove all the calibration tools.
3.2 MECHANICAL ADJUSTMENT OF THE INJECTION FORK – SN > 149 (only if required)
This calibration consists of adjusting the injection fork on its plastic bracket – SN > 149.
Note: If the standby current is disable, no need to follow both screenshots below.
Select “Test Mode”, "Injec." and click on a direction for each motor to disable the standby current (verify that you can move
the injection fork without risk before clicking on a direction).
Move manually the injection fork to get some access.

Remove the anode tank holder (two screws) and the anode tank.
Loosen the three screws holding the injection fork on its holder and push it against the distributor motor metal plate
(underneath the distributor). When the injection fork is aligned on the metal plate, tighten the screws and place back the
anode tank.
3.3 DRAINING POSITION
Note: If the standby current is disable, no need to follow both screenshots below.
Select “Test Mode”, "Injec." and click on a direction for each motor to disable the standby current (verify that you can move
the injection fork without risk before clicking on a direction).
Move manually the injection fork to get some access.

Loosen both screws holding the EV9-12 block, move down the filling/draining metal pipe and tighten the screws.
Note: this script will automatically customize P1854 (filling position). Please note the value of this parameter before to do the
draining position calibration and replace it manually after the calibration to its previous value.
From the “Advanced Settings”, select “Script”, “Injection” and “RemplNelleCuve1”.

Answer “No” to the question below and adjust the draining position using the four arrows below:
Anode tank
draining position
If the two capacitive pins are not centered (too close of the anode tank pin) or not aligned with the anode tank, loosen both
screws holding the anode tank filling/draining assembly and adjust it. Once the adjustment is done, tighten the screws.
Adjust the front/back position of the anode tank so the two Adjust the height of the anode tank to get the metal tubing of the
capacitive sensors are centred inside the anode tank. filling/draining nearly touching the bottom of the anode tank.
Note: the anode tank must still be free on its holder, try to move
it using the mechanical play of the anode tank on its holder
3.4 FILLING POSITION
Using a syringe, fill the anode tank with P6 buffer or water (only if P6 buffer is not available):
- OCTA: 6.5mL
- TERA: 9mL
Select “MiniCycle” - “Fluidic Anode”, tick the boxes “Injection fork to origin”, “Anode tank in filling/draining position”, select
“buffer” and “draining”, then press on “Start”.
Select “Command” – “Motors” – “Inj. Z”:
To reach the home position of the

injection Z:
- Steps: 4000
- Hold Current: Enable
- Direction: “Down”
- Press “home position”
To reach a position:
Direction
- Steps: start with 1100 and
increase (check the current value
of P1854 to have a reference)
- Hold Current: Enable
Home Position Start
- Direction “Up”
- Press “Start”
Go to the “Test Mode” (see next page) to

verify if the liquid has been detected.
Make trials with different values until the

liquid is detected (go back to the home
position before to do a trial with another
value)
The purpose is to adjust the height of the anode tank so the two capacitive pins are detecting the liquid.
To verify if the liquid has been detected, select “Test Mode” – “Fluidics” – “Sensors”:
- Liquid not detected: value “0” and
- Liquid detected: value “255” and
Once the correct number of steps is known:
Customize the following parameters to adjust the height of the anode tank with the new capacitive sensor:
- P1854 (filling into the buffer compartment) => number of steps to detect the liquid
- P1876 (filling into the wash compartment) => P1876 = P1854 + 20
Note: Do not move/slide or adjust the 2 capacitive pins of the sensor.
3.5 DILUTION SEGMENT TRANSFER
Place a dilution segment on the distributor tray (a transparent one is provided with the instrument, it is easier)
From the “Advanced settings”, select “Script”, “Injection” and “SaisieBarrette1”.
The step 1 is only a “pre-adjustment”, press “Next”.
During the step 2:

- Use the up and down arrows to place the segment on the injection fork but only few Millimetres above the distributor tray.
If the segment is not aligned, rotate the distributor.
- When the segment is aligned, adjust the front/back position of the injection fork to pick-up correctly the segment.
Use the up/down arrows to test the segment transfer, it must be smooth/without shaking.
- Before to press “Next”, place back the injection fork to the default height (in brackets).
Segment transfer
3.6 ANODE TANK UNDERNEATH CAPILLARIES (TERA)
ADJUSTMENTS MUST BE DONE WITH THE THERMOSTATING BLOC (PELTIERS, RADIATOR, FANS) PLACED ON THE TOP OF THE CAPILLARIES
Make sure the Distributor tray is into its origin position (home position), prior running the script “NewCuveLavageCapils”.
From the “Advanced settings”, select “Script”, “Injection” and “NewCuveLavageCapils”.
Anode tank
underneath
capillaries
The two electrodes must be aligned with the anode tank.

If necessary, remove the capillaries and move the front electrode (screws highlighted in red) or the cathode tank (screws
highlighted in blue) until the electrodes are aligned:
Move the anode tank to get the electrodes in the middle of the tank and then adjust the depth of the electrodes into the
anode tank:
The height must be adjusted to get the platinum

electrodes in contact with the bottom of the anode tank,
when the anode tank is touching, move down the
injection fork of 110 steps (one rabbit and one turtle).
Once this calibration is done, refer to the section 5. 3.5

PARAMETERS to adjust the other parameters (other
cleaning positions) as detailed below:
P1841 = P1839 - 50
P1842 = P1852 = P1839 + 50
3.7 ANODE TANK UNDERNEATH CAPILLARIES (OCTA)
The injection fork must be parallel to the front electrode. The left top part should touch the front electrode but the anode
tank locker should not. Please find below the example to follow:
When the left top part is in contact with the front electrode, move 100 steps down and save the calibration.
Once this calibration is done, refer to the section 5. 3.5 PARAMETERS to adjust the other parameters (other cleaning
positions) as detailed below:
P1841 = P1839 - 50
P1842 = P1852 = P1839 + 50
3.8 DILUTION SEGMENT INJECTION (TERA)
Remove all the segments on the loading area and place a transparent segment (provided with the instrument) on the loader.
Note: cut half part of the first and last wells of one segment or use the transparent segment
From the “Advanced settings”, select “Script”, “Injection” and “InjectionCapils”.
Segment
injection
Answer “Yes” to load a segment from the loader to the injection fork and start the calibration cycle.
Answer “No” to go to the second question.
Place manually a segment on the injection fork and answer “Yes” to the second question to start the calibration cycle.
Answer “No” to the second question to abort the calibration.
Use the front/back arrows to position the injection fork so the capillaries are in the center of the segment wells, then move
the injection fork up until the capillaries are at the correct depth into the segment wells (see picture above).
Note: Use a mirror to see the capillary n°1 (left). See the capillary n°12 (right) through the hole above the monochromator
3.9 DILUTION SEGMENT INJECTION (OCTA)
On the OCTA, during the injection, the two electrodes will dive in the wells 1 & 12.
We recommend performing two times the injection height calibration, first time to adjust the capillaries alignment and
depth, 2nd time to verify the electrode alignment and depth.
1st time: 2nd time:
4. SYRINGE
4.1 SYRINGE INSTALLATION
Select “Fluidic” from the “Mode Test”, select “Syringe M17” and click on “Origin”.
The syringe motor will move to its origin position:
Follow the manufacturer's recommendations to assemble the syringe:
Place the spacer on the syringe (do not put a white o’ring, it Screw only the top part of the syringe by hand, tighten one
is already inserted in the production line on the black plastic quarter revolution further using pliers:
part above the syringe, a blue tape is there to avoid that the
white o’ring falls during the shipment):
Spacer
Syringe must not be

tightened on the
motor during force
sensor calibration
Black
O’Ring
4.2 FORCE SENSOR CALIBRATION
Pull down the syringe piston of few millimeters before to adjust the force sensor.
Please refer to the section 7. 1. 6 & 8 WAY VALVES CALIBRATION to remove the thermal block and pull out the fluidic metal
sheet.
With a screwdriver, tighten or untighten very slowly in order to adjust the threshold of the force sensor within 5000 and
7000 (never overpass 10000, it could break the force sensor) and put some blocking varnish to block the position.
Place the black o’ring on the syringe motor’s screw and tighten the syringe’s piston on its motor using the Minicap key.
Note: be careful to maintain the syringe well tightened on the top when tightening the piston to the syringe motor.
4.3 FORCE SENSOR TEST
Press “home position” to move the syringe totally down and then “Force sensor zero volume position”. The syringe must go
totally up, without dead volume and without hitting too hard the top part of the syringe.
Position 1 Position 2
5 RACK HOLDER
5.1 BELT
To insure a correct movement of the racks on the belt, it might be necessary to adjust the tension of the belt
The gap between the nut and the extremity of the endless screw is 4 or 5 mm.
Decrease the gap → the belt will be more stretched (more tension)
Increase the gap → the belt will be less stretched (less tension)
5.2 RACK LOADING
Select "Racks" from the “Test Script” and select “EntreeDessusTapis”.
Step 1: Adjustment of the rack to have the bottom pin aligned on the inlet guide.
Then place a sample rack on the inlet tray (in front of the rack entry detection sensor) and click on “Next”.
Step 2: Calibration to load the rack on the rack holder.

Height adjustment (Motor Z) of the Rack in order to obtain a gap of 2 mm between the sample rack carrier and the pin of the
rack, check this adjustment for both sides of the rack.
Click only on indicated directions and then “Next”.
2 mm
Step 3: Height adjustment of the rack for the transfer to the dilution position.
12 mm
5.3 RACK UNLOADING
Remove the rack before to perform this calibration cycle. Place it back on the rack holder as soon as the initialization is over
and the rack holder is placed on the outlet position.
Step 1: Adjust the rack position in order to obtain the same position as on the picture below and click on “Next”.
1 mm
3 mm
Step 2: Adjust the height of the rack to obtain 2mm between the
sample rack carrier and the pin of the rack.
Then click on “Next”.
2 mm
Step 3: Adjust the rotation of the sample rack holder to release the rack.
Note: On the production line, the value applied is 560 for all the instruments.
Then click on “Next” to save the calibration.

Note: Be careful, the sample rack holder must not touch the secondary reagent assembly when coming back to its home
position!
6 NEEDLE ARM
All the needle arm calibrations are located in “Test Script” and “Needle – Bar code reader”.
6.1 TUBE
Place one 5mL tube with an Eppendorf reducer on position 2 of a rack and a conical tube for controls and calibrators without
cap on position 3.
Place one customer’s tube (with or without cap) on position 1 of the rack and another one without cap on position 8.
Place this rack on the conveyor and start the calibration cycle “Tube1”.
The first step is used to adjust the position of the sample probe above the tube on position 1.
The second step is to adjust the maximal depth of the needle used during the DCN calibration.
The third step is to adjust the maximal depth of the needle used with the rack “0” (calibrator and control).
The fourth step is to verify that the needle is well centred above the tube on position 8, if not please renew this script and find a
compromise between tube 1 and 8 or check the needle arm alignment with the rack.
Note: Move down the needle manually to check the centring of the needle above the tube in position 1.
Note: The depth of the needle into the capped sample tubes is the parameter P2378 (by default, it is equal to P2376 – 3000).
6.2 WELL
Place a segment on the distributor (position 1) before to start this calibration script.
Follow the flowchart to adjust the needle arm position and if needed the distributor angle.
Well 12 Y
Well 1
Step 1: adjust the needle above the well 1.
Step 2: adjust the depth of the needle into the well 1, the needle should almost
touch the bottom of the well.
Step 3: verification of the centring of the needle above the well 12.
If the needle is not centered, redo trying to find a compromise between the
well 1 and the 12 or verify, and renew if needed, the mechanical adjustment of
the home position of the distributor.
6.3 DILUENT
Place a 100mL container (without cap or with the pierceable cap) on position S1 before to start the calibration script.
The first step consists of calibrating the needle above the diluent 1.
During the second step, move down the needle to almost touch the bottom of the diluent container.
The diluent container must be free, it must be possible to slide it up around 1 or 2 mm.
6.4 CLEANING
First step is to adjust the needle

centring above the cleaning tube
(10mL).
Note: Move down manually the

needle to be more precise.
Step 2 consists of adjusting the

needle height inside this cleaning
tube. It must be free, let 1mm of
play between the needle and the
bottom of the tube.
6.5 ANTISERA TUBE
During the step 1, adjust the needle above the antisera tube 1.
The step 2 consists of calibrating the height of the needle to reach the bottom of the tube.
6.6 IT PREDILUTION TANK
Start by centering the needle above the IT pre-dilution well and

adjust the height of the needle to reach the bottom of this well.
The needle should be as close as possible of the bottom of the well

without touching it.
6.7 HBA1C CAPILLARY TUBES
From the “Script” menu, press on “Specific operations” and select “MicroTubeCalibOperation.sh”.
Place a rack Cx with two capillary sample tubes (remove and cut the white caps) in positions 1 and 8 on the conveyor belt,
against the rack stoppers.
Confirm the two following messages:
The sample probe goes to the sampling position 1 and the following message is displayed:
Adjust the sample probe height by pressing on “+/-“ (20 steps)

or “Yes/+ / No/-“ (200 steps).
Press “Quit” to continue the adjustment, the sample probe

goes to the sampling position 8.
Verify, re-adjust if necessary and press “Quit” to continue.
The sample probe goes back to the sampling position 1 and

the following message is displayed:
20 steps
200 steps Next Press to restart the adjustment or press to

stop the script.
Exit
When stopping the script, the following message is displayed:
Press to save the parameters and unload the rack, then press to leave the script.
7 BARCODE READER
7.1 CALIBRATION: RackTubeNReadingCalib.sh
At the very beginning, the Capillarys 3 is testing the serial connection with the barcode reader.
If the connection is Ok, it is displaying “Barcode reader detected!”
If the connection is not Ok, the script is asking for turning “On” the barcode reader and configuring it.
Place a rack with 8 tubes with different barcodes in the inlet conveyor.
The rack will be loaded and taken to the dilution position.
Front Back
If necessary, adjust the verticality of the
aim by bending a little bit the white metal
bracket holding the camera of the barcode
reader.
Adjust the tilt of the barcode reader by

clinking in “+/-” button to get the
horizontal line in the middle of the barcode
label.
Adjust the pan of the barcode reader to get

the vertical line on the front side of the
barcode label (as shown on the picture
beside) by rotating the white metal bracket
with the two little screws on the top.
The rack must be pushed against the back side of the

rack holder when adjusting the aim (Red Cross laser)
Press “Change/Test” to read the barcode labels.
When the reading is good, close both conveyor lids and press “Quit” to end the cycle.
After the unloading of the rack, press the door icon to close the window and go back to the scripts list.
7.2 DEFAULT SETTING
Important: the default settings used by Sebia is different from the one given by the manufacturer.
Do not use the “default setting” of the barcode reader manual.
Using the default SEBIA setting, the validated barcode families are Code 39, Code 128, ITF (Interleave Two by Five).
If needed to re-initialize the barcode reader, from the “Advanced settings”, select “Barcodes” and press “Default setting”:
7.3 MODIFY THE SETTING
Reach the “advanced settings”, select “Barcodes”.

The barcode reader manual is available in the Sebia Service Knowledge Base
Read the manual (section “symbologies”) to get the parameter’s number and the value you have to apply according to the
customer’s barcode labels (family, length, check-digit…).
Parameter number
Value
Selected parameter
Value to apply to the selected

parameter
Save the value of the selected

parameter
See the value of the selected

parameter (yellow)
Most used settings:
ITF (Interleave Two by Five): minimal length (16) and maximal length (17)
Codabar:
- Activation (7) to 1
- Minimal length (18) and maximal length (19)
- Remove the start and stop digits (37) to 1
Code 39: minimal length (12) and maximal length (13)
Example (to setup the barcode reader to accept the reading of ITF barcodes with 12 digits only):
- Enter “16” into the yellow block, “12” into the green block and press on the blue block to save the value.
- Enter “17” into the yellow block, let “12” into the green block and press on the blue block to save the value.
7.4 INSTALLATION OF A NEW BARCODE READER
First time using a new barcode reader, it must be initialized before use.
Place the barcode reader in the instrument and plug its flat cable; then switch “ON” the instrument.
Reach the “Script” – “Leds, lecteur CBs tests” and start “InitBarCodesReader.sh”:
8 DCN
It is mandatory to perform a Capiclean cycle prior to perform the DCN calibration.

Into a sample rack, place an empty 5mL tube on position 1 with an Eppendorf micro-tube filled with 100µL of distilled water
inside this tube (in case of false detections, perform the calibration with 150µL).
Place the rack on the conveyor belt.
Important: the rack must be in front of the inlet opto sensor (On the left side of
the inlet conveyor).
From the “Advanced Settings”, select “MiniCycle” - “DCN”, tick the two highlighted cycles, select the position of the used
buffer container (P6 buffer if possible) and press on “Start” to perform the DCN calibration.
When the cycle is done, verify that the values displayed are corrects:
- Threshold (DetThresh) must be within 20 and 40
- compIDAC must be within 70 and 120
When the values are correct, tick the box “Save DCN parameters” and press “Start”:
9 CATHODE TANK
9.1 FILLING SENSOR (C24)
The platinum wire of the cathode (red line) could disturb the sensor if it is placed in front of it.
To avoid any disturbance, it must pass underneath the sensor, as shown on the picture.
To adjust the level of liquid into the tank (blue line):

- customize the threshold (P3130 => increase the value of the parameter to decrease the volume)
- or adjust manually the position of the sensors (emission and reception) to obtain a correct filling
Liquid level
Platinum wire
9.2 OVERFLOW SENSOR (C32)
The values are not exactly the same from one sensor/instrument to another one.
From the “Advanced settings” reach the “MiniCycle”, select “Fluidic Cathode”, tick the box “Calibrate Overflow Sensor”,
select “Overflow” and press “Start”.
SECTION 8: GLOSSARY
SECTION 8: GLOSSARY
CAN (Bus):
Controller Area Network bus is a communication bus designed to allow microcontrollers and devices to communicate with
each other in applications without a host computer. It allows managing an important quantity of microcontrollers and
sensors, with a fast bit rate.
DHCP:
The Dynamic Host Configuration Protocol is a standardized network protocol used on Internet Protocol (IP) networks. The
network configuration parameters are dynamically distributing (such as IP addresses for interfaces and services).
With DHCP, computers request IP addresses and networking parameters automatically from a DHCP server, through the
network.
No need for the network administrator, or for the user, to configure these settings manually.
IHM:
Interface Human-Machine designates all the visual and graphical aspect displayed in order to inform and interact between a
system and the operator.
KiTTY:
It’s a free and open source terminal emulator, serial console and network file transfer application.
It’s a fork from the version 0.64 of PuTTY. KiTTY is only designed for Windows platforms.
PuTTY:
It’s a free and open-source terminal emulator, serial console and network file transfer application.
It supports several network protocols (including SSH for instance)
It can also connect to a serial port (since version 0.59)
SSH:
Secure SHell (SSH), is a cryptographic (encrypted) network protocol for initiating text-based shell sessions (interface sessions)
on remote machines in a secure way.
This allows a user to run commands on a machine's command prompt without them being physically present near the
machine. It also allows a user to establish a secure channel over an insecure network in client-server architecture.
The standard TCP port 22 has been assigned for using SSH communication.
VNC:
Virtual Network Computing (VNC) is a graphical desktop sharing system that uses the Remote Frame Buffer protocol (RFB) to
obtain the remote control from one computer on another computer.
The link is made through a network connection.
VNC is PC platform-independent. So it can be used on any PC operating System.
SECTION 9: SCHEMATICS
SECTION 9: SCHEMATICS
1- ELECTRONICS
1.1 CAPILLARYS 3 TERA (SN < 149) - CAN BUS & POWER SUPPLY
1.2 CAPILLARYS 3 TERA (SN < 149) - LAYOUT & WIRING
1.3 CAPILLARYS 3 TERA (149 ≤ SN ≤ 220) - CAN BUS & POWER SUPPLY
1.4 CAPILLARYS 3 TERA (149 ≤ SN ≤ 220) - LAYOUT & WIRING
1.5 CAPILLARYS 3 TERA (221 ≤ SN ≤ 518) - CAN BUS & POWER SUPPLY
1.6 CAPILLARYS 3 TERA (221 ≤ SN ≤ 518) - LAYOUT & WIRING
1.7 CAPILLARYS 3 TERA (SN ≥ 519) - CAN BUS & POWER SUPPLY
1.8 CAPILLARYS 3 TERA (SN ≥ 519) - LAYOUT & WIRING
1.9 CAPILLARYS 3 OCTA - CAN BUS & POWER SUPPLY
1.10 CAPILLARYS 3 OCTA - LAYOUT & WIRING
2- FLUIDICS
SECTION 10: DIFFERENCES FROM TERA TO OCTA
SECTION 10: DIFFERENCES FROM TERA TO OCTA
Front electrode:
Cathode Tank:
Anode tank:
Reception fibers:
Emission fibers:
Hydraulic hub 6x2:
Fibers locker:
Temperature regulation (2x foams to replace capillaries N° 1, 2, 11 & 12):
Anode tank DCN pins (same part number but different wiring):
Anode tank locker:

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CAPILLARYS 3 TERA & OCTA

Version Author Date

- FILLING POSITION – Page 74

- SCHEMATICS – Page 115

SECTION 1: WARNING ........................................................................................................................... 5

SECTION 7: ADJUSTMENTS & CALIBRATIONS ................................................................................. 57

The instrument has been designed to be used indoors.

Do not use the instrument near water or in wet locations.

• Adjustments, alterations or servicing have been made by authorized personnel.

1 Not intended for direct connection to telecommunication networks

• 3.2 GHz Intel® Core i5 processor, Windows® 7 (32 or 64 bits)

• 23-inch SVGA screen with a definition of 1920 x 1080

• Memory RAM 4.0 GB

• Graphic card 65536 colors

• Ports: USB (printer, keyboard, mouse, barcode reader, scanner), RJ45

• Two Ethernet (network) cards

Figure 1: Front view

2. EXTERNAL HARDWARE DESCRIPTION

- On the front panel (see figure 1):

- On the right panel (see figure 1):

• 5 - micro-plate compartment door.

- On the rear panel:

- On the left, reagent lid opened:

• Primary reagent compartment.

- In the middle, reagent lid opened:

• Fluidics metal panel,

• Secondary reagent compartment.

- On the right, reagent lid opened:

- At the back, rear cover removed:

1.3 MODULE POSITION

2.1 BLOCK DIAGRAM

START LID SETTINGS SHUTDOWN

Capillarys 3 TERA & OCTA – Service Manual

CUSTOM MIGRATION ACQUISITION MOTORS

v1.15 P1 – Date: March 18th, 2019

2.1 MAIN MENU

First menu displayed when turning ON the Capillarys 3.

Displays main reagent

Cycle’s name and status

Opens the lid Displays the settings

To validate and return

To validate and return

Click on the name of the cleaning cycle to be performed.

1. Normal shutdown (regular daily priming)

2. Rinsing shutdown (normal shutdown + rinsing fluidics with water)

When installing Capillarys 3, check the date and time.

Click on “DHCP” to switch from dynamic to static IP address.

Set-up the connection on Phoresis (available only when Phoresis is OFFLINE):

This menu allows the customer to:

- customize some warning levels,

Same principle for the automatic shutdown and cleaning cycles.

Select “Parameters” from the “Advanced settings” menu. Search by number

Modification zone: Enter the value

Modify: click to change the value of

Customize: click to change the value

Replace: to replace the default value

Upper part of the test mode is dedicated to the High Voltage:

Second part is dedicated to the capillaries temperature:

Note: 45°C must be reached in 60 seconds (from ambient temperature)

• The one on the left is to move a motor to its origin.

The status of the DCN is displayed on the bottom of this tab.

- Origin, close to 7000.

- No rack detected, close to 7000.

- No rack RF-ID tag detected.