BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

### : Significance of HMG CoA

Viva Question Q51 reductase
2nd year Tca cycle
Purines degradation
Hormones of ant pituitary
External: Dr Suhaib Farooq (MMDC) Lyesh nehan syndrome
Internal: Dr Naveed Najeeb (QAMC) ### : GSD
Diabetes insipidus
### : Urea cycle kis kis organ mn hti Hormones of posterior pituitary
Conns syndrome Alkaptonuria
Beta oxidation ### : Acth function
Topoisomerase inhibitors Burnt diabetes
### : Urea cycle complete Oncogenes
Disease of urea cycle Cancer agent in urinary bladder
Regulation of urea cycle Virus causing cancer
Glycogen degradation Why bp raise in Cushing’s syndrome
Insulin ka main function. No. Of insulin like growth factor
Cloning significance ### : Rna polymerase
FAS steps Hyper ammonia
### : GSD Refsum
Phenylketonuria Glycogen synthesis
B oxidation ### : Regulatory step of glycolysis
Addison disease ### : Urea cycle complete
Estrogen k effects Disease of urea cycle
### : Refsum disease Regulation of urea cycle
Rate limiting steps of glycolysis Glycogen degradation
Cytochrome p450 Insulin ka main function.
Hypothyroidism HHH syndrome
### : Orotic aciduria Cloning significance
HMP FAS steps
Cushing syndrome
### : Mostly internal assessment k
### : Types of hormones
base pay question pochty hin..
Urea cycle
### : Agr arginase ki deficiency hojy
Function of HMG CO A reductase
to kia hoga( arginosuccinate ki
Imp of this step
accumulation hojy gi )
Cushing syndrome
### : Refsum disease

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Rate limiting steps of glycolysis pathways with xenobiotics

Cytochrome p450 Retrovirus with 2 example
Hypothyroidism ### : External
### : GSD Pheochromocytoma
Phenylketonuria Conns disease
B oxidation PTH
Addison disease ### : External
Estrogen k effects Regulation of thyroid hormones
### : Albinism Cause of diabetes mellitus when
### : Orotic aciduria there is increased ACTH
HMP
### : Internal:
Cushing syndrome
### : Types of hormones
HMP shunt
Urea cycle
Its products
Function of HMG CO A reductase
Refsum disease
Imp of this step
Harmons of post. Pituitary
Cushing syndrome
Hirsutism
### : Significance of HMG CoA
Albinism
reductase
Post Transcriptional Modifications
Tca cycle
Purines degradation
Hormones of ant. pituitary
External:
Lyesh nehan syndrome
### : GSD
HMP shunt products
Diabetes insipidus
Regulatory steps of HMP
Hormones of posterior pituitary
Disease name bcz of deficiency of
Alkaptonuria
G6PD
### : Von gierkes mn hyperuricemia
What other complication occur with
kasy hta
hemolytic anemia
Gout
Jaundice types name...how
Uric acid levels
prehepatic occurs?
### : External..
DHEA? abbreviation
Hypothyroidism k patient ko kesy
Androgen names
pata chaly ga by appearance
A patient with tachycardia,
Konsay test hoty hypothyroidism mn
hypertension, insomnia.... diagnosis?
TSH level (first normal then increase)
### : G
How can we relate the uronic acid
Blood sampling Wala Just batana tha

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Centrifuge perform krna tha uses

Firstly on kara Urea cycle with defects of 1st 2 steps
Rpm set kara ### : External
Time set kara Glycogenolysis
Tubes balance kara Fatty acid synthesis
### : *Long practical* Albinism
Urea Tca cycle
Uric acid Alpha keto glutamate
Bilirubin Cortisol synthesis
Total protein Zellweger syndrome
Albumin Western blotting
Cholesterol Albert syndrome
### : Blood glucose Hartnup disorder
Internal: hypoglycemia Hyper natremia
hyperglycemia effects? Gamma glutamyl cycle
Methods for glucose estimation orotic aciduria
External: hypoglycemic shock Histidine metabolism
### : Serum Total protein Tryptophan metabolism
Qs.. ### : ext.. lesch nyhan
internal: hartnup
reagent blotting types
principle tryptophan metabolism
where protein synthesized? int.... urea cycle
hyper& hypo proteinemia causes git hormones
pcr
External: ### : Internal
hypoproteinemia Urea cycle
in which condition protein loss from B oxidation
body? Fructose metabolism
### : Serum cholesterol W oxidation
External: if a person is allergic to External
statins, what alternative drugs can Restriction endonuclease
be administered. Lactic acidosis
### : Internal ### : Internal:
Regulation of gene expression full Pancreatic juice
chapter Chyme
Hmp shunt full significance N ADPH Diabetes milletus and types

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Lipoproteins ### : Non observed

Lynch syndrome Which acids form neurotransmitters
Xeroderma pigmentosa Types of lipoprotein
Concept of uncoupling Enzyme for Degradation of
ETC complexes lipoprotein and it's cofactor
Cushing syndrome Aminotransferase and their value
Diabetes insipidus Level of cholesterol
Why liver cannot use ketone body 2 tests for cholesterol
for energy? Reverse cholesterol
Git hormones Names of branched chain amino
### : Internal: acids
Phenylketonuria Anticoagulants
Glycolysis regulation ### : Testosterone production
Replication fork Maple syrup syndrome
Macrophages me foam cells ki Cori’s cycle
formation Gamma glutamyl cycle
Cushing CTP(cytidine triphosphate) forfamtin
### : Internal: glucogen synthesis Leshnyhan syndrome
Alkaptonuria Application of PCR
Orotic aciduria
External: ### : External:-
Digestion of proteins Bioenergetics ki definition
Aik bnday ny khaana khaaya energy
### : Internal
kesy milay gi usy ?
Translation process poora
Metabolic Alkalosis
Arginase ki deficiency sy kia hoga
Glycogen storage diseases
Git kay hormones
### : External
External
RFLP
Endopeptidase
DNA proof reading
Glucose 6 phosphatase ji deficiency
VLDL role
sy kia hota
Purine salvage pathway
### : External
Post translational modifications
Post translational modifications
Protein translation termination
Internal ### : External...
Hmp shunt wth imp
Anion gap
Mutations and types (only names)
Respiratory alkalosis
Dna repair mechanisms (names)
Tumor suppressor gene

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Addison's disease ### : External

Cushing syndrome What happen after well fed state?
### : External Cholesterol synthesis
RFLP Internal
Cloning Chemiosmotic hypothesis
Inhibitors of protein synthesis Maple syrup urine disease
### : Genetic code Refsum disease
RNA polymerase ### : Internal
FAS synthesis Uronic acid pathway
Orotic aciduria Gene
Cystic fibrosis Types of gene
Ketolysis Cholesterol synthesis
Copy sa sun rhy hn Thyroid hormone synthesis
### : External ### : Internal
XP Glycolysis k regulatory steps
Xanthine Oxidase inhibitor CPS 1 r 2 ka difference
Internal Transcription k inhibitors
Glycogen Synthesis Protein hormones ka Mechanism of
Cholesterol Synthesis action ( dno ne pcha )
### : Internal Net Energy of palmitic acid
Alkaptonuria Xenobiotics
TCA cycle External
Inhibitors of topoisomerase Rho independent r dependent
External What is promoter
Orotic aciduria G proteins system
SCID ### : Internal
Ketolysis Hormone classification
### : *Internal* : Plasmid
Alkaptonuria Urea cycle
Krebs cycle ### : Internal
Anterior pituitary hormone Homocystinuria
*External* Fatty acid synthesis
Cholesterol regulations Ectopia lentis
Chylomicrons Urea cycle k enzymes r defects
Cholelithiasis ### : External
Homocystinuria Operons

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Internal Rafsum disease

BUN and it level Phenylketonuria
Urea level Hyperthyroidism
Urea cycle kahan hota hai Lipoprotein lipase
Uric acid formation Inhibitor of ETC and there complex
Salvage pathway ADA k function
Glycogenolysis Orotic aciduria
### : Internal
Inhibitors of ETC Chill scene
Uncouplers Sir cooperative hn kafi
Difference b/w inhibitors and ### : Internal
uncouplers Topoisomerase inhibitors
Glycogen storage diseases Urea cycle
Alkaptonuria Glycolysis regulated steps
### : *External* Cytochrome p450 function
Fatty liver ### : Internal
Insulin synthesis + hyperinsulinemia Krebs cycle steps
Restriction endonucleases Alkaptonuria
Topoisomerases Phospholipids ki degradation
*Internal* ADA Deficiency
Glycogen storage diseases ### : *INTERNAL*
Farber disease xeroderma pigmentosum
Purine de Novo synthesis lecyh nyhan syndrome
Adenosine deaminase deficiency Fatty acid synthesis
Location of carbomyl phosphate *EXTERNAL*
synthetase 1 fatty liver............
### : External ### : Biochem practical creatinine
copy khol k samney jo aya wo sunlia What is creatinine?
2 sawal, zada baten ki aur bhej dia Creatinine clearance
Internal Prerenal, renal and post renal cause
Hogness box of increase in creatinine
Refsum disease ### : Internal
MSUD Translation complete with inhibitors
Glycolysis irreversible steps Glycoproteins
HGPRT deficiency External
### : Internal Protein targeting

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Free energy change Gigantism

Albinism Food intake ... digestion
### : Internal Composition of gastric juice
Names of glycogen storage diseases Internal
Mutation and types pribnow box
Homosysteinuria.. enzyme deficient Lipids storage disease
symptoms Cox 1 and Cox 2
### : External Steroid hormone
Conn's syndrome ### : Internal
Hertnup disease • Glycogen Storage Disease
Main focus Performa r copy Sy hi • Lipid Storage Disease
sunty • Cyclo oxygenase Pathway, and it's
Internal inhibitors
Urea cycle enzymes defeciencies and • Lesch Nehan Syndrome
diseases • Purine Salvage Pathway
Resume disease • Purine Degradation
Plasmid • De Novo Synthesis of Purines
Glycogen synthesis • Phenyl Alkaptonuria
### : Apoliproterin types • HHH syndrome
Centrifuge precaution • What is differences between
Nonstandard amino acids of urea Uncouplers and inhibitors
cycle • What two processes are actually
CK types and disorder uncoupled and how does it happen
Creatinine kinase formation ### : Internal:
Uric acid level Anterior pituitary hormones
Calcium levels tetany Lesch nehan syndrome
### : *Internal* Thyroid hormone synthesis
Pyruvate dehydrogenase complex Tyrosine products
Citric acid cycle Albinism
Hyperthyroidism
*External* External:
Just checked progress card Tyrosine kinase/ insulin working
Then asked kuch sunao gy? mechanism
Chlo Conn’s syndrome bta do Regulation of gene expression
### : External copy wala ache se kr k jana... wahin
Operon se sun rahe
### : Internal:

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Glycogenolysis process Retrovirus with 2 example

ETC inhibitors ### : External
Initiation phase of replication Regulation of thyroid hormones
enzymes Cause of diabetes mellitus when
XP there is increased ACTH
Lesch nyhan syndrome ### : Refsum’s disease
Topoisomerases Homocystinuria
2 3 or they lkn sb genetics se they Chemiosmotic hypothesis
wo kr lena Respiratory alkalosis
External: ### : Acth function
Chilomicrons Burnt diabetes
Lipoproteins Oncogenes
Cholelithiasis Cancer agent in urinary bladder
### : Von gierkes mn hyperurecimia Virus causing cancer
kasy hta Why bp raise in crushing syndrome
Gout No. Of insulin like growth factor
Uric acid levels ### : External
### : HbA1c ... presentation ka Fructose metabolism
question tha Celiac sprue
Tyosin ki fate Lipid digestion
### : Significance of HMG CoA Internal
reductase Glycolysis
Tca cycle Uraea cycle
Purines degradation Pribnow box
Hormones of ant.pituitary Post pit harmone
Lyesh nehan syndrome
### : External
### : GSD
Chylomicrons
Diabetes inspidus
Purines degradation
Hormones of posterior pituitary
Lesch nyhan
Alkeptonuria
Hypogonadism
### : External.. Retroviruses
Hypothyroidism k patient ko kesy Internal
pata chaly ga by appearance
Branch chain amino acids
Konsay test hoty hypothyroidism mn
metabolism
TSH level (first normal then increase)
Chyle and chyme
How can we relate the uronic acid
Steroid hormones mechanism of
pathways with xenobiotics
action

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

Prostaglandin synthesis DNA gyrase

### : Internal ### : (Internal)
Beta oxidation #Lipids storage diseases
Palmitate sy kitny ATP bnty #Effect of LH and FSH on males
Post translational modification #Neimann pick disease
### : Water intoxication #Homocystinuria effects
Acidosis and organs involved in acid
base balance (External)
GSD’s Transcription steps
PCR
Cushing disease vs cushing syndrome Boys ka chill scene hi tha External
### : GSD.. waly main
Degradation of phospholipase...
### : External
Translation inhibitor..
Con syndrome
Pribnow box ..
Acromegaly
Electron transport chain ..
Aur sath main ye Kay Tum Sab larkay
### : *Internal*
kyn nahi parhtay
### : Internal: glycolysis steps,
Gluconeogenesis Precursors
Anterior pituitary hormones,
Adrenal hormones
glucokinase Vs hexokinase, ..
Functions of Androgens
Hodgness box ### : External: citric acid cycle
Prostaglandin synthesis w inhibitors steps, cytochrome p450, essential
Phenylketonuria amino acids, Transcription definition.
### : *Internal* ### : External: genetic basis of
ETC inhibitor disease and examples
Cholstreol synthesis secondary aldosteronism
Steroid hormone mechanism of
action Internal: inhibitors of ETC
### : internal classification of hormones
glycogen storage disease k name biomedical importance of urea cycle
phenylketonuria types of hyper ammonemia
anterior pituitary hormone k name post transcriptional modification
cholestrol synthesis ### : External
### : Internal Inhibitors of purine synthesis
Fatty acid synthase Regulation of hmp shunt
Complex 5 Internal

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

GSDs Substance P
Alkaptonuria Malate shuttle
Refsums disease ### : Internal:
Diff b/w Cushing syndrome and Cholesterol synthesis
disease Uncouplers
### : External Albinism
1. genetic bases of disease Hogness box
2. diabetes insipidus External:
Internal Pancreatic hormone
1. erythromycin Somatostatin
2. translation Enkephalin
3. GSDs Substance P
### : External : dopamine Neurotoxin
brakdown Cyp P450 hydroxylase
internal: ### : External
gsds Hybridization
translation Gene extraction
### : External DNA cloning
Intermittenary metabolism Internal
Internal Glycogen synthesis
Glycogen synthesis Git harmones
BCAA degradation Pribnow box
Maple syrup urine disease Cons syndrome
Translation Hyperammonemia
### : Uncouplers ### : External: primosome, general
2-3 BPG definition from genetics
Mutation Internal: purine synthesis, HMP
Refsum disease. shunt, translation with its inhibitors
### : Genetic cuases ### : Internal...
HCG hormone ETC
### : Internal: Inhibitors of ETC
Rate limiting step of glycolysis. Translation
Ant. Pituitary hormone Hyperthyroidism
Lesch neyhan syndrome. External....
Intestinal juice
External: Tumor suppressor genes

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

analogs(purine,nucleotide) Transport of ammonia 2 ways

External copy sai hi sun rhy hain Topoisomerase inhibitors
sara External:
### : Internal Corrie cycle RFLP
Homocystenuria Biotransformation
Cholesterol metabolism steps ### : External: Copy K.E level ki bni
### : Internal: hui haii, sawaal bhi K.E level ke
Hmp shunt, G6PD deficiency, ketone puchun ga
bodies, TCA , arachidonic acid cycle, Regulation of gene expression in
post transcriptional modification, prokaryotes and eukaryotes
synthesis of fructose, phenyl FIGLU
ketonuria, cystinuria GABA ka K.E level ka answer do.
External: Histidinemia
why TCA cycle is anaplerotic, hmp ### :
shunt regulation, what is ogpt, ALT ~Internal: Uronic
function acid pathway Maple syrup
### : Internal....GSD,LSD urine disease GIT
names,urea cycle and defects and hormones
hogness box (names) ~Exter
### : External.....pribnow box,free nal: Oxidative
energy, placental Harmones deamination Hydrogen
### : External pump Metabolic
Thermogenin disorders Ketogenesis
HMP shunt
Internal regulation Cholesterol
synthesis
Uronic acid pathway
Beta oxidation ### : internal
Translation types of lipoproteins
beta oxidation of fatty acids
### : Internal:
precursors of gluconeogenesis
Hormones of Git and their functions
amino acids that undergo
2,3 BPG ki importance
gluconeogenesis (glucogenic)
Beta oxidation of fatty acids
normal urea level and cholesterol
And 2 3 or bhi the...
level
### : Internal:
types of blotting
Glycogen storage diseases names
External:
Urea cycle purpose

QAMC Study Stuff…………………………………………………………21/02/2023

 BIOCHEM PROFF VIVA QUESTION 2ND YEAR Q51

no viva Akhir mn kaha parhty nhi hoo 🥲

### : Internal ### : Non observed mn jo
Glycogen storage disease questions aye unn mn se kuch ye
Fatty acid synthesis thy..
Pheochromocytoma
External ALT and ALP ki serum value male mn
Glycogenolysis
### : Chemiosmotic theory Creatinine ki serum value aur urine
Etc inhibitors mn value
Fatty acid synthase domains
Pheocytochromia 4 causes of hypercalcemia
Pcr
External Specifically Myocardial infarction mn
Synthetic derivatives of purines kon se CK aur LDH ke isoenzymes
Target proteins raise hoon ge.
### : INTERNAL
chemiosmotic theory Free cholesterol gene regulation ke
Alkaptonuria through kin 2 proteins ki formation
Gout and its types ko inhibit karta
Glucagon mechanism of
action Fasting glucose value for normal
adult, define renal threshold of
EXTERNAL
glucose and its value
Regulation of HMP shunt
PCR process
4 adrenal hormones formed from
### : Internal
cholesterol
Uronic acid pathway
### : Observed mn glass pipette use
Refsum disease
karna tha (micropipette ni) aur 5 ml
Cushing syndrome and diasease
diluted serum solution banana tha..
RFLP
uss mn ye karna tha keh takreeban 1
PCR
ml sample lena aur 4 ml serum ka,
External unka solution banana tha phir..
Send up ka paper smna tha first
question koya
What is hunter syndrome baaki Colorimeter tha, uss mn
HMP shunt is regulated by wavelength set karnii thi 530 nm ki..
What is hemophilia ###

QAMC Study Stuff…………………………………………………………21/02/2023