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  • Restriksi Enzim

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    Tyson Simorangkir77
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    The document contains restriction mapping data from four Macaca species - M. fascicularis, M. mulatta, M. tonkeana, and M. fuscata. It includes primer sequences, sequence sizes, and restriction enzyme analysis. The analysis shows the number of cut sites and positions for enzymes that cut the sequences (e.g. AatI cuts M. fascicularis at position 112) and lists enzymes that were selected but did not cut. A table summarizes the results across the four species.

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    Restriksi enzim

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    The document contains restriction mapping data from four Macaca species - M. fascicularis, M. mulatta, M. tonkeana, and M. fuscata. It includes primer sequences, sequence sizes, and restriction enzyme analysis. The analysis shows the number of cut sites and positions for enzymes that cut the sequences (e.g. AatI cuts M. fascicularis at position 112) and lists enzymes that were selected but did not cut. A table summarizes the results across the four species.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    12 views3 pages

    Restriksi Enzim

    Uploaded by

    Tyson Simorangkir77
    The document contains restriction mapping data from four Macaca species - M. fascicularis, M. mulatta, M. tonkeana, and M. fuscata. It includes primer sequences, sequence sizes, and restriction enzyme analysis. The analysis shows the number of cut sites and positions for enzymes that cut the sequences (e.g. AatI cuts M. fascicularis at position 112) and lists enzymes that were selected but did not cut. A table summarizes the results across the four species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 3

    Macaca fascicularis

    No mispriming library specified


    Using 1-based sequence positions
    OLIGO start len tm gc% any 3' seq
    LEFT PRIMER 191 20 59.97 50.00 6.00 0.00 CCTCAATTGCACACATCACC
    RIGHT PRIMER 350 20 59.94 45.00 2.00 0.00 ATGCCGATGTTTCAGGTTTC
    SEQUENCE SIZE: 1141
    INCLUDED REGION SIZE: 1141

    Table by Enzyme macaca fascicularis

    Enzyme No. Positions Recognition


    name cuts of sites sequence
    AatI 1 112 agg/cct More
    info
    AvaI 1 19 c/ycgrg More
    info

    The following endonucleases were selected but don't cut this sequence:

    AluI, ApaI, BamHI, EcoRI

    Macaca mulatta

    No mispriming library specified


    Using 1-based sequence positions
    OLIGO start len tm gc% any 3' seq
    LEFT PRIMER 744 20 60.10 55.00 2.00 0.00 CCTCCTAAACGACCCAGACA
    RIGHT PRIMER 909 20 59.94 55.00 4.00 2.00 GGCTGCTAGAATGAGGATCG
    SEQUENCE SIZE: 1141
    INCLUDED REGION SIZE: 1141

    Table by Enzyme macaca mulatta

    Enzyme No. Positions Recognition


    name cuts of sites sequence

    The following endonucleases were selected but don't cut this sequence:

    AatI, AluI, ApaI, AvaI, BamHI, EcoRI

    Macaca tonkeana

    No mispriming library specified


    Using 1-based sequence positions
    OLIGO start len tm gc% any 3' seq
    LEFT PRIMER 842 20 60.15 45.00 4.00 0.00 TACGATCCGTCCCCAATAAA
    RIGHT PRIMER 1040 20 59.92 50.00 2.00 0.00 AGGGGTTGGGTTATTGGTTC
    SEQUENCE SIZE: 1141
    INCLUDED REGION SIZE: 1141

    Table by Enzyme macaca tonkeana

    Enzyme No. Positions Recognition


    name cuts of sites sequence

    The following endonucleases were selected but don't cut this sequence:

    AatI, AluI, ApaI, AvaI, BamHI, EcoRI

    Macaca fuscata

    No mispriming library specified


    Using 1-based sequence positions
    OLIGO start len tm gc% any 3' seq
    LEFT PRIMER 87 20 59.97 50.00 3.00 0.00 ATGGTGGAACTTTGGCTCAC
    RIGHT PRIMER 241 20 60.04 55.00 7.00 2.00 AGCGAGTGACTCAGCCGTAT
    SEQUENCE SIZE: 1141
    INCLUDED REGION SIZE: 1141

    Table by Enzyme macaca fuscata

    Enzyme No. Positions Recognition


    name cuts of sites sequence
    AatI 1 57 agg/cct More
    info
    AvaI 1 123 c/ycgrg More
    info

    The following endonucleases were selected but don't cut this sequence:

    AluI, ApaI, BamHI, EcoRI


    Tabel Hasil Peta Restriksi dari Empat Spesies Macaca

    Jenis Macaca
    Macaca mulatta Macaca fuscata Macaca tonkeana
    Enzim Fascicularis
    Posisi Jumlah Posisi Jumlah Posisi Posisi Posisi Posisi
    Pemotongan Pemoto Pemotongan Pemoto Pemotongan Pemotong Pemotongan Pemotong
    Enzim ngan Enzim ngan Enzim an Enzim Enzim an Enzim

    AatI 112 1 - - 57 1 - -

    AluI - - - - - - - -

    ApaI - - - - - - - -

    AvaI 19 1 - - 123 1 - -

    BamHI - - - - - - - -
    EcoRI - - - - - - -

    Kesimpulan :

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