ABSTRACT

Ika Fatmawati.S (A1L118005), Phytochemical Test and Antioxidant Activity of

Ethyl Acetate Extract of Belimbing Wuluh (Averrhoa bilimbi L.) Leaf with DPPH
Method (supervised by Drs. Haeruddin, M.Si. and Wa Ode Mulyana, S.Si., M.Sc.)

A study on "Phytochemical Tests and Antioxidant Activity of Belimbing

Wuluh Ethyl Acetate Extract (Averrhoa bilimbi L.) Using the DPPH Method" was
carried out with the aim of knowing the types of secondary metabolites identified in
the ethyl acetate extract of Belimbing Wuluh leaves and to determine the
antioxidant activity of the ethyl acetate extract of Belimbing Wuluh leaves. starfruit.
250 grams of belimbing wuluh leaves were macerated with technical ethyl acetate
solvent and a thick 12.62% ethyl acetate extract was obtained. To determine the
group of secondary metabolites, the phytochemical method was used and the
antioxidant activity was determined by the DPPH and DPPH methods. The ethyl
acetate extract of belimbing wuluh leaves is known to contain alkaloids, steroids,
tannins and has very low antioxidant activity with an IC50 value of 547.56 ± 0.0196
ppm, while vitamin C has a very strong antioxidant activity with an IC50 value of
2.33 ± 0. 1786 ppm. Based on the two IC50 results between ethyl acetate extract of
starfruit leaves and vitamin C, we can see that the ethyl acetate extract of starfruit
leaves has antioxidant activity which is much lower than the antioxidant activity of
vitamin C.

Keywords: Antioksidants, Belimbing Wuluh Leaves (Averrhoa bilimbi L.),DPPH,

Vitamin C.