Introduction to Analytical Chemistry

Introduction
Analytical chemistry is the science of obtaining, processing, and communicating
information about the composition and structure of matter. In other words, it is the
art and science of determining what matter is and how much of it exists. or it define
as studies and uses instruments and methods used to separate, identify, and
quantify matte , So it is the science that concerned with the separation,
identification, and determination of the relative amounts of the components making
up a sample.

Applications of Analytical Chemistry

Analytical chemistry used in many fields:
 In medicine, analytical chemistry is the basis for clinical laboratory tests
which help physicians diagnosis disease and chart progress in recovery.
 In industry, analytical chemistry provides the means of testing raw materials
and for assuring the quality of finished products whose chemical
composition is critical. Many household products, fuels, paints,
pharmaceuticals, etc. are analysed by the procedures developed by analytical
chemists before being sold to the consumer.
 Enviermental quality is often evaluated by testing for suspected
contaminants using the techniques of analytical chemistry.
 Forensic analysis - analysis related to criminology; DNA finger printing,
finger print detection; blood analysis.
 Bioanalytical chemistry and analysis - detection and/or analysis of
biological components (i.e., proteins, DNA, RNA, carbohydrates,
metabolites, etc.).

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Classification of analysis
Qualitative analysis: An analysis in which we determine the identity of the
constituent species in a sample.
Quantitative analysis: An analysis in which we determine how much of a
constituent species is present in a sample.

Sample analysis
Analytes: The constituents of interest in a sample.
Matrix: All other constituents in a sample except for the analytes.

Classifying Analytical Techniques

Classical techniques
Mass, volume, and charge are the most common signals for classical techniques,
and the corresponding techniques are:
1- Gravimetric techniques.
2- Volumetric techniques.
3- Coulometeric techniques.

Instrumental techniques
1- Spectroscopic methods - measuring the interaction between the analyze and
electromagnetic radiation (or the production of radiation by an analyze).

2- Electroanalytic methods - measure an electrical property (i.e., potential, current,

resistance, amperes, etc.) chemically related to the amount of analyze.

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Basic Tools and Operations of Analytical Chemistry
Basic Equipment
Measurements are made using appropriate equipment or instruments. The array of
equipment and instrumentation used in analytical chemistry is impressive, ranging
from the simple and inexpensive, to the complex and costly.
Equipments for Measuring Mass (Analytical Balance)
An object’s mass is measured using a balance. The most common type of balance
is the mechanical balances which are replaced by the electronic balances.

Electronic balance

Equipment for Measuring Volume

Volumetric flask is designed to contain a specified volume of solution at a stated
temperature, usually 20 °C.

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Pipette is used to deliver a specified volume of solution. Several different styles of
pipets are available.

Burette is volumetric glassware used to deliver variable, but known volumes of

solution. A burette is a long, narrow tube with graduated markings, and a stopcock
for dispensing the solution.

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Equipment for Drying
Many materials need to be dried prior to their analysis to remove residual
moisture. Depending on the material, heating to a temperature of 110–140 °C is
usually sufficient. The processes can be accomplished using a laboratory oven
capable of providing the required temperature,

Conventional laboratory oven used for drying materials.

Example of a muffle furnace used for heating samples to maximum
temperatures of 1100–1700 °C.

Filtration
In gravimetric analysis, the mass of product from a reaction is measured to
determine how much unknown was present. Precipitates from gravimetric analyses
are collected by filtration

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 Filtering a precipitate.The conical funnel is supported by a metal ring attached to
a ring stand, neither of which is shown.

Preparing Solutions
Preparing a solution of known concentration is perhaps the most common activity
in any analytical lab. Two methods for preparing solutions are described in this
section. A stock solution is prepared by weighing out an appropriate portion of a
pure solid or by measuring out an appropriate volume of a pure liquid and diluting
to a known volume.

Preparing Solutions by Dilution

Solutions with small concentrations are often prepared by diluting a more
concentrated stock solution. A known volume of the stock solution is transferred to
a new container and brought to a new volume.

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Methods for the expression of concentration
1-Mole: Sympol (mol)
Is defined as the quantity of given substance that contains as many molecules for
formula units as the number of atoms in exactly

Number of moles for compounds =

Note: Molecular weight of compounds is the sum of the atomic weight of all the
atoms in the molecular formula of the compounds

For example: number of moles of (NH2)2CO =

Number of moles of ion = , for SO4 =

Number of moles of element = , Ag =

2-Molarity: symbol (M)

When we dissolve a substance in a liquid, we call the substance (solute) and the
liquid (solvent), so the molarity of solution defines the number of gram-molecular
weight (or) moles of solute dissolved in 1 liter of solution, or the number of milli
molecular weight in 1 millimeter of solution

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For solid sustances

Thus:

Notes: (wt) means weight in gram unit (gm)

(vol) means volume of solution in milliter unit (ml)
For liquid substances

Also

Example: 1
What is the molarity of a solution containing (16 gm) CH3OH in 200 ml of
solution ? M.wt =32.

Sol.
M= x

= x

=2.5 mol/L

Example:2
Calculate the molarity of H2SO4 solution of specific gravity 1.198 , containing 27
% H2SO4 by by weight ?
At.wt. of H=1 , S=32 , O=16 .

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Sol.
M.wt of H2So4 = (2x1)+32 +(4x16)=98 gm/mol

M=

=
=3.3 mol/L

3- Normality : symbol (N)

The normality of a solution expresses the number of milliequivalents of solute
contained in 1 ml of the solution, or the number of gram equivalents contained in 1
liter.

N=

Number of gram-equivalent weight =

Note: (eq.wt) means equivalent weight. (its unit is )

So, for solid substances

N= x

And, for liquid substances

N=

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 Example :1
How many gram-equivalent of solute are contained in 0.5L of 0.2 N solution?
Sol.

N=

0.2= = 0.1 gm. Equivalent of solute

Example: 2
How many grams of solute are required to prepare 1 liter of 1N solution of NaCl ,
M.wt = 58.45 ?
Sol.

N= x

=58.45 gm.

Note: N=n M
Which , N=Normality
n= Valence number or equivalence number *
M=Molarity
*Valence number of an element is the number of atoms of hydrogen (or its
equivalent ) which one atom of the element combines with or displaces. So, in NaCl:
M.wt =eq.wt
But in H2SO4: eq.wt= M.wt/2 because there are 2 hydrogens (protons) which
displaces.

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Example3

What will be the normality of 3 molar solution of calcium hydroxide Ca(OH) 2?

Solution:

M= = = 1.5 N

Calculation of n
HCl=1 , H2SO4=2 , H3PO4=3, NaOH =1, Ca(OH)2=2 ,
Al(OH)3 =3, CaCl2=2, Na2SO4=2 , Na2CO3=2, CaSO4=2

4-Percentage concentration:
The percentage composition of a solution can be expressed in several ways.
Three of the common methods are defined as follows:

Weight percent = x100

Volume percent = x100

5-parts per million concentration :

For very diluted solution, the concentration is more conveniently expressed is
part per million (ppm). This term is defined by equation:

ppm = x 106 or mg/L

ppm = x 106

Thus, an aqueous solution containing 0.0003 % nickel by weight contains 3ppm of

nickel by weight. How?
0.0003% means 0.0003 gm solute in 100gm solution.

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So, ppm = x 106

= x 106
=3 ppm
The relation between (ppm) and (molarity) is
ppm= molarity x molecular weight x 1000

The relation between (ppm) and (normality) is:

ppm = normality x equivalent weight x 1000

Example:
Aqueous solution of NiCl2 with a concentration of 500 ppm, What is the molarity
and normality of this solution ? At.wt of : Ni=58.69 , Cl=35.5

Diluting solutions:
Example:
You are given a solution of 14.8 M NH3 , How many milliliters of this solution do
you require to give 100ml of 1M NH3 when dilute ?

In this kinds of examples and if you need to get a diluted solution, follow the
dilution equation :
Mi Vi = Mf Vf
14.8 Vi = 1 100
Vi = 6.76 ml

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Home work

Question: 45.57 mL of a solution is diluted to 63.40 mL. The diluted solution is

found to have a concentration of 0.433 N. What was the concentration of the
original solution?

Question: Calculate the weight of Calcium hydroxide present in 250 ml of two

normal solution?

Question: If 40 grams of NaOH with an equivalent weight of 40 is dissolved in

one liter of a solution, does the normality of the solution is one, give the
calculation?

Question: Calculate the molarity of 0.650 N HCl.

Question: What will be the normality of 3 molar solution of calcium hydroxide?

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