Exercise for part I

I. Direction: Each question below contains one correct answer. Choose the most
suitable response to each question.

1. Which one is a basic amino acid?

A. asp B. ala C. his D. trp E. glu

2. Which one is the most important bond to make the secondary structure of protein
more stable?
A. Hydrophobic bond
B. Ionic bond
C. Hydrogen bond
D. Van der Waals force.
E. Disulfide bond.

3. The enzyme will lose its biological activity when heated, why?
A. The enzyme become amino acid
B. Damaged of primary structure
C. Damaged of spatial structure
D. Lose its solubility
E. Lose activator

4. Which group is consisted all by essential amino acids?

A. leu, phe, lys, ile
B. val, ser, thr, ile
C. gly, tyr, his, asp
D. trp, met, phe, cys
E. ala, lys, thr, leu

5. Which of the following amino acids has positive charge at physiological Ph ?

A. Cys B. Glu
C. Lys D. Trp E. Gly

6. Allosteric enzymes are:

A. monoenzymes B. oligomeric enzymes
C. multienzyme system D. michaelis-menten enzyme

7. hn RNA refers to :
A. the collection of pre-mRNAs B. the collection of pre-tRNAs
C. the collection of pre-rRNAs D. the population of snRNAs
II. Fill up the blanks.

1. The specificity of enzyme-catalyzed reactions includes ___________,

_______________, and _______________.
2. The competitive inhibitor binds reversibly to the active site of an enzyme. Thus
there is_______________ in the Vmax of the enzyme, but Km _______________.
3. The sequence of a DNA fragment is GCTACTAAGC, the base sequence of its
complementary strand is _______________.
4. Three amino acids _______________, _______________ and _______________
contribute to most of the ultraviolet absorbance of proteins, which absorb
maximally at 280 nm.
5. Enzyme can be grouped into _______________,
_______________,_______________,_______________,_______________,
_______________ classes.
6. G-6-P is the branch point for several metabolic pathways, they are
, , and
etc.
7. The mechanism of favism is involved in the deficiency of
(full name of the enzyme).

III. Explain the following concepts.

1. motif
2. Tm value
3. DNA renaturation
4. exon
5. PI
6. peptide unit
7. zymogen
8. isoenzyme
9. the active site of an enzyme
10. feedback inhibition
11. covalent modification

IV. Answer the following questions.

1. What are the action forces maintaining primary, secondary, tertiary, and quaternary
structures of proteins?
2．Please describe the structure of DNA.(double helix)
3．What is enzyme inhibition? What are competitive and noncompetitive inhibition?
What’s the difference between them? Please give an example.
4．Please compare the function of m RNA, r RNA, t RNA.
5．What is michaelis-Menten equation and describe the Km.
6 ． Please write the whole name of the abbreviations and which Vitamine are
includable:

TPP, FAD, FMN, NADH, NADPH, CoA.