Literature Presentation

1. 4~8 members / team;

2. Present Major Points in English (with necessary Chinese explanation);
3. PPT with contribution of each member;
4. Deadline: 11th Jun. Sent to E-mail: ilmare13007@163.com
百合固金汤对结核 Regulations of Bai He Regulation of Baihe Gu
杆菌感染后巨噬细 Gu Jin Tang Drug- Jin Decoction
胞自噬功能的调节 serum on autophagy Medicated-serum on
作用 in macrophages Autophagy in
【摘要】 目的 观 infected by Macrophages with
察百合固金汤对巨 Mycobacterium Mycobacterium
噬细胞内自噬功能 tuberculosis tuberculosis Infection
的调节作用，以探 [Abstract] Research [Abstract] Objective:
寻其可能的抗结核 on regulations of Bai To explore the acting
分枝杆菌作用机制 He Gu Jin Tang mechanism of Baihe Gu
。 Drug-serum on Jin Decoction against
autophagy in mycobacterium
macrophages in order tuberculosis through
to definite the possible observation of its
mechanism of this regulation on autophagy
decoction against in macrophages.
Mycobacterium
tuberculosis.
方法 以百合固金汤 Bai He Gu Jin Tang Methods: This study used
medicated-serum of
含药血清干预感染结 Drug-serum interfered
RAW264.7 Baihe Gu Jin Decoction
核分枝杆菌的 to interfere with
RAW264.7巨噬细胞， macrophages infected RAW264.7 macrophages
0h、6h、12h、24h、 by Mycobacterium infected with
tuberculosis. After 0h, mycobacterium
36h后RT-QPCR法检
6h, 12h, 24h and 36h, tuberculosis, employed
测干预前后巨噬细胞 detecting the mRNA
RT-QPCR to detect the
atg5、atg7、atg8、 expression of atg5, atg7, mRNA expressions of
atg12表达变化；12h、atg8, atg12 by RT- atg5, atg7, atg8 and atg12
24h、36h后Western QPCR; After 12h, 24h 0h, 6h, 12h, 24h and 36h
Blot法检测干预前后 and 36h, detecting the after intervention
respectively, and adopted
巨噬细胞内自噬相关 protein expression of
Western Blot to testify
蛋白LC3-Ⅱ的表达 LC3-Ⅱ by Western
the autophagy-relevant
差异；12h、24h、 Blot and DNA content
protein expression of
36h后QPCR法检测 of Mycobacterium LC3-Ⅱand QPCR to
tuberculosis in
用药前后胞内结核分 quantify the DNA content
macrophages by of Mycobacterium
枝杆菌DNA含量。
QPCR respectively. tuberculosis 12h, 24h and
36h later correspondingly.
结果 药物干预24h后， Compared to control
用药组巨噬细胞atg5、 group, the Bai He Gu Jin
atg7、atg8、atg12表达 Tang drug-serum group
量出现不同程度增加 significantly increased the
(P<0.05)，自噬特异性 mRNA expression of atg5,
蛋白LC3-Ⅱ表达量亦显 atg7, atg8, atg12 and the
著增高(P<0.05)；药物 protein expression of
干预36h后，在自噬特 LC3-Ⅱ after 24h(P<0.05).
异性蛋白LC3-Ⅱ表达量 While after 36h, we found
显著增高(P<0.05)的同 Bai He Gu Jin Tang
时检测到胞内结核分枝 drug-serum significantly
杆菌DNA含量明显减少 up-regulated the protein
(P<0.05)。 expression of LC3-
Ⅱ(P<0.05), and down-
regulated the DNA
content of Mycobacterium
tuberculosis in
macrophages (P<0.05).
Results: Compared with
control group, there was
significant increase in both
the mRNA expressions of
atg5, atg7, atg8 and atg12 of
macrophages (P<0.05) and
the specific autophagy
protein expression of LC3-
Ⅱin the medicated-serum
group 24h after
intervention (P<0.05); while,
notable up-regulated
protein expression of LC3-
Ⅱ(P<0.05) was detected to
coexist with distinct down-
regulated DNA content of
Mycobacterium tuberculosis
in macrophages (P<0.05)
36h after intervention.
结论 百合 All the results come Conclusion: Baihe
固金汤可能 to the conclusion Gu Jin Decoction
通过激活细 that Bai He Gu Jin may assist in treating
胞自噬现象 Tang maybe treat tuberculosis through
发挥抗结核 the tuberculosis by activating autophagy
的作用。 activating in macrophages.
【关键词】 autophagy in [Keywords] Baihe
百合固金汤 macrophages. Gu Jin Decoction;
；自噬；结 [Key words] Bai He Autophagy;
核分枝杆菌 Gu Jin Tang； Mycobacterium
；巨噬细胞 Autophagy； tuberculosis;
Mycobacterium Macrophages
tuberculosis；
Macrophages
Effect of Xuefu Zhuyu Decoction on
endothelial function in
血府逐瘀汤对冠心病高同型
hyperhomocysteinemia of
Clinical Observation of Xuefu Zhuyu
Decoction Effect on Endothelial Function
半胱氨酸血症血管内皮功能
coronary artery disease: a in Hyperhomocysteinemia of Coronary
影响的临床随机对照研究
randomized controlled clinical Artery Disease
trial
【摘要】目的 To
Abstract：Objective 观察血府逐
study the Abstract：Objectives To study the
intervention function of Xuefu Zhuyu
瘀汤联合西药治疗冠心病高
clinical effect of Xuefu Zhuyu
Decoction combined western medicine on
Decoction combined with western
同型半胱氨酸血症的临床疗
medicine in endothelial function in
效及其对血管内皮功能的影
hyperhomocysteinemia of Hyperhomocysteinemia of coronary artery
coronary artery disease and its disease．Method 80 patients with
响。方法efficacy on将80例冠心病高
endothelial function. Hyperhomocysteinemia of coronary artery
同型半胱氨酸血症患者随机
Methods Eighty patients with disease were divided into treatment group
hyperhomocysteinemia of
分为治疗组与对照组，每组
coronary artery disease were
and control group，40 cases in each group
。The two groups were all given
40例。两组均予单硝酸异山
divided into treatment and control
groups, with 40 cases in each Isosorbide Mononitrate Tablets and
梨酯缓释片、阿司匹林肠溶
group. The two groups were both Aspirin Enteric-coated Tablets，the
片口服，对照组加用叶酸片
given Isosorbide Mononitrate control group were add in Folic Acid，the
Tablets and Aspirin Enteric-coated
，治疗组加用血府逐瘀汤。 treatment group were add in Xuefu Zhuyu
Tablets, with additional Folic Decoction。
Acid for the control group and
Xuefu Zhuyu Decoction for the
treatment group respectively.
两组疗程均为3个 The Period of treatment of With an intervention of three
all groups were three months, the serum
月，观察监测治疗 months，and then observe levels of Hcy, NO,
前后受试者的血清 the levels of Hcy、 NO、 iNOS, SOD, MDA,
Hcy、NO、iNOS、 iNOS、SOD、MDA、 vWF and Ps in
SOD、MDA、 vWF、Ps in plasma after subjects were observed
being treated．Result ① before and after the
vWF、Ps水平的 Within the group after treatment. Results ①
情况。结果 ①治 treatment，the differences For pre and post
疗前后组内比较， of the levels of Hcy plasma treatment comparison
两组同型半胱氨酸 all have statistical within each group,
significance(P<0.05)； there was statistical
水平差异均有统计 comparison between significance in the
学意义（P＜ groups after treatment， differences of the Hcy
0.05）；组间治疗 the Hcy levels difference levels (P<0.05); for
后比较，同型半胱 was statistically significant comparison between
，the treatment group is groups after treatment,
氨酸水平差异有统 better than the control the (changes of )Hcy
计学意义，治疗组 group (P<0.05)； levels in the treatment
优于对照组（P＜ group were lower than
those in the control
0.05）。
group (P<0.05).
② For Intra-group comparison:
②治疗前后组内比较，两组 ②Within the group after treatment，
significant difference was
NO、iNOS、SOD、MDA、 the differences of the levels of NO、
observed between the levels
vWF、Ps水平差异均有统计
of NO, iNOS, SOD, MDA, iNOS、SOD、MDA、vWF、Ps all
vWF and Ps (P<0.05) prior to have statistical significance (P<0.05)，
学意义（P＜0.05）；组间治
and after intervention; for comparison between groups after
疗后比较，NO、iNOS、SOD、
group comparison after treatment，the NO、iNOS、SOD、
MDA、vWF、Ps水平差异有
treatment: the levels of NO, MDA、vWF、Ps levels difference wer
统计学意义，治疗组优于对照
iNOS, SOD, MDA, vWF and statistically significant，the treatment
Ps in the treatment group
组（P＜0.05）。结论 血府逐 group is better than the control group
were significantly lower than (P<0.05)。Conclusions The treatment
瘀汤联合西药治疗冠心病高同
those in the control group
of Hyperhomocysteinemia of
型半胱氨酸血症，疗效满意，
(P<0.05). Conclusion Xuefu
Coronary Artery Disease by Xuefu
可显著改善患者的血管内皮功 Zhuyu Decoction combined western
Zhuyu Decoction with western
medicine was effective in
能，降低血清同型半胱氨酸水
improving the endothelial medicine was satisfactory ，which can
平。 function and reducing the significantly improve the endothelial
serum homocysteine levels in function of patients,and reduce the
patients with serum homocysteine levels。
hyperhomocysteinemia of
coronary artery disease.