Professional Documents
Culture Documents
Requirement
Reagent preparation;
for 1000 ml saline preparation
1000ml = 58.5 gm of Nacl
so, for 100ml = 58.5/1000 100
5.85gm Nacl
5.85 gm of Nacl in 100ml distilled water is added to make 100 ml of saline
Inoculum preparation
1. using a sterile inoculating loop or noodles touch four or five isolated colonies of
the organism to be tested
Principal
Protocol
1.Prepare the nutrient agar media and autoclave the media sline and glasswares.
3.Inoculate the entire agar surface of each plate, first in horizontal direction and then in
vertical direction to ensure the even distribution of the bacteria over the agar surface,
using the swab.
7. Sterile forceps were used to press the disc in place was marked by antibiotic name.
1. Examine all the plates for the zone of inhibition surrounding the discs.
2. Measure diameter of zone of inhibition in mm, using the ruler on the underside of the
plate.
3. Record the zone size and prepare a graph comparing the zones obtained with the
known concentrations versus the zone of unknown.
4. Find out the approximate number of penicillin units in the culture filtrate.
Fig-3 Fig-4
Precautions