Nutrient#

Starch Test
reagent : iodine [ yellow brown blue /black]

I ( break
boil
leaf in water down cell walls ) 130sec )

2.) place in hot akhol ( remove chlorophyll) 13min)

) V
3.) rinse with water 130 sec

4.) plate leaf on tile .

Using a dropper , drop a few dropsof iodine

Glucose Test
reagent : Benedicts
reagent

1) Blue Solution no traces

place a small sample
of solution into a test tube

Green /yellow traces

2.) add 2m /
of Benedicts into each test tube of reducing sugar

3.) place in water bath for 10min orange red moderate

4) observe colour
change
Brick -
red
large amount
of reducing sugar

Protein Test :
Biuret A ( copper sulphate) and Biuret Bl either potassium / sodium
reagent :
hydroxide)

1.) add a small amount

of food sample to test tube

2.) add 1mi Biuret A 1m /of Biuret B

of and using a
syringe

3.) place in a water bath for 10min

4) observe colour changes

positive changes to
purple

negative
colour stays blue