Colegio de Sta. Lourdes of Leyte Foundation Inc.

Tabontabon,Leyte
College of Nursing

SAPROPHYTES CULTURE
USING BAMBOO AND
RICE / CULTIVATING BIO
ORGANIC FERTILIZER
THROUGH THE USE OF
BENEFICIAL BACTERIA
SUBMITTED BY:
RAAGAS, JELLA MARIE
PABO, JANE MARTIN
QUILAQUIL, JENNY MAE
TUMBAGA, ELANIE
CELSO, JANELLA
OBENA, CLAIRE

SUBMITTED TO:
DR. RUSTICO B BALDERIAN
I.INTRODUCTION

Composting is often described as an aerobic process in which organic waste materials are
degraded by activities of microorganism into much simpler nutrients and produce organic
fertilizer at the end of the process. It is a decomposition process which involves succession of
different microbial population to breakdown organic matter and used as their energy supply.
Decomposition of biodegradable organic wastes by microorganism community changes physical
and chemical parameters of compost. In order to produce mature compost in a short time,
presence of correct microorganism is needed. This improvement process can be done with the
addition of IMO into compost to enhance the composting process. IMO is a beneficial member
of the soil microorganism including bacteria, yeast and fungi collected from non-cultivated soil.
It has a high content of microorganism in the soil. Since microbial community played an
important role in decomposed organic matter and lead to compost production, study upon
diversity of microorganism during composting process in necessary. In this study we are
producing IMO from cooked rice and molasses to identify the effect of IMO produce on the
composting process.

II. OBJECTIVES

 Promote plant health and growth by providing plants with beneficial microorganisms that aid in
nutrient absorption, root development, and disease resistance.
 Enhance soil fertility by decomposing organic matter, increasing nutrient availability, and
promoting nutrient cycling.
 Suppress harmful pathogens, pests, and diseases by introducing beneficial microorganisms that
outcompete or inhibit their growth, providing a natural defense mechanism for plants.
 Accelerate the composting process and help in the efficient breakdown of organic waste
materials, leading to the production of high-quality compost for soil enrichment.
 Promote sustainable agriculture by reducing reliance on synthetic fertilizers and pesticides,
promoting natural soil and plant health, and minimizing environmental impact.

III. MATERIALS
 Bamboo
 Rice
 Tupperware
 Mollases
 Biodegradable material
 Rice cooker
 Spoon
  Mask
 Gloves
 Manila Paper
 Rubber band

VI. PROCEDURE
1. Remove the green outer layer of the bamboo and make an opening. Keep the removed part of the
opening to use as a lid.
2. Sterilizes the bamboo’s inner and outer part.
3. Cook a cup of rice in a rice cooker and let it cool.
4. Put the rice in a cleaned bamboo tube and cover it with a manila paper as the first layer and a long
plastic cellophane as the second layer, each layer being tied on both sides with rubber bands.
5. Place the covered bamboo into a dim and cool area for 3-5 days, letting the good (white) molds to grow
and multiply.
6. Remove the moulds together with the rice from the bamboo and place it in a container and have an
enough molasses.
7. Measure the rice of moulds as well as the molasses, both having the same measurement.
8. Mix the rice of moulds and the molasses and store it for 14 days.
9. After the storage period, filter the mixture in a strainer. Place the strained product into a container and
mix it with waste materials; specifically dried leaves and sayote peels. Then wait for one month to
determine its effectivity on decomposing waste materials.

V. RESULTS AND CONCLUSION

When our group made the bamboo and rice culture, it took us several efforts to complete it since
there are always black molds that grows in every culture that we cultivate. Our group thought that the
reason could be that we always wait 5 days before checking for our culture. Our group attempted to make
another bamboo and rice culture, but this time we only waited 3 days before seeing the results of what we
cultivated. After those 3 days of waiting, there were no black molds that grew, which confirmed what we
had thought. It was the only challenge that our group encountered when doing the culture. When we
achieved the rice culture, we collected the rice with indigenous microorganism on it and placed it in a
container with the molasses and biodegradable materials. After that, the microorganisms consume the
biodegradable material, converting it into simpler forms and releasing by-products. These by-products are
normally non-toxic and can be used by other organisms or contribute to ecosystem nutrient cycle.
In summary, the microorganisms break down and change the biodegradable materials added to
the IMO into simpler organic molecules, which are then absorbed by the bacteria themselves, returning
essential nutrients to the environment. Our group completed the work properly and effectively by
following the instructions provided to us. A reason to believe in the successful cultivation of bio-organic
fertilizer through the application of beneficial bacteria.