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CLASS XII (2019-20)


BIOLOGY (044)
SAMPLE PAPER-1

Time : 3 Hours Maximum Marks : 70


General Instructions :
• There are a total of 27 questions and five sections in the question paper. All questions are
compulsory.
• Section A contains question numbers 1 to 5, multiple choice questions of one mark each.
• Section B contains question numbers 6 to 12, short answer type I questions of two marks each.
• Section C contains question numbers 13 to 21, short answer type II questions of three marks
each.
• Section D contains question numbers 22 to 24, case-based short answer type questions of three
marks each.
• Section E contains question numbers 25 to 27, long answer type questions of five marks each.
• There is no overall choice in the question paper. However, internal choices are provided in two
questions of one mark, one question of two marks, two questions of three marks and all three
questions of five marks. An examinee is to attempt any one of the questions out of the two
given in the question paper with the same question number.

Ans : (a) be rapidly pumped back from


Section A sedimentation tank to aeration tank
1. In majority of sexually reproducing or
organisms, the gametes are [1]
(a) isogametes (b) homogametes AIDS is caused by HIV. Among the following,
which are the modes of transmission of HIV ?
(c) hemigametes (d) heterogametes
(i) Transfusion of contaminated blood
Ans : (d) heterogametes (ii) Shaking hands with infected people
(iii) Sexual contact with infected people
or
(iv) Sharing of food with infected people
Autogamy is defined as the Choose the correct answer from the options
(a) transfer of pollen grains from the anther given below
to the stigma of the same flower (a) (ii) and (iv) (b) (i) and (iii)
(b) transfer of pollen grains from the anther (c) (ii) and (iii) (d) (i) and (ii)
to the stigma of the different flower
Ans : (b) (i) and (iii)
(c) maturation of anther and stigma at
different times 3. A bacterial cell was transformed with a
(d) dehiscence of anther and release of pollen recombinant DNA that was generated using
grains a human gene. However, the transformed cell
did not produce the desired protein. Reason
Ans : (a) transfer of pollen grains from the
could be [1]
anther to the stigma of the same flower
(a) human protein is formed but degraded by
2. Activated sludge should have the ability to bacteria
settle quickly so that it can [1] (b) human gene may have intron which
(a) be rapidly pumped back from bacteria cannot process
sedimentation tank to aeration tank (c) amino acid codons for humans and
(b) be discarded and anaerobically digested bacteria are different
(c) absorb colloidal organic matter (d) All of the above
(d) absorb pathogenic bacteria present in Ans : (b) human gene may have intron which
waste water while sinking to the bottom bacteria cannot process
of the settling tank
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4. Identify organisms A and B that are shown in increase of algal growth imparts a distinct
the diagram of organismic response below [1] green colour to the water body (algal bloom).
These blooms cause deterioration of the
water quality leading to fish mortality.
7. The pedigree chart given below shows a
particular trait which is absent in parents but
present in the next generation irrespective of
sexes. Draw your conclusion on the basis of
the pedigree. [2]

(a) Organism A- Conformer; Organism B-


Regulator
(b) Organism A- Regulator; Organism B-
Ans :
Partial regulator
(c) Organism A- Conformer; Organism B- The pedigree chart shows that the trait is
Partial regulator autosome-linked and recessive in nature. But,
the parents are carriers (i.e. heterozygous)
(d) Organism A- Partial regulator; Organism
hence, among the offspring, only few show the
B - Conformer
trait irrespective of sex. The other offspring
Ans : (a) Organism A- Conformer; Organism are either normal or carrier.
B - Regulator
8. Sterilisation techniques are generally
5. An antibiotic resistance gene in a vector considered as the full proof contraceptive
usually helps in the selection of [1] method with least side effects. Then, why
(a) recombinant cells (b) competent cells couples keep this as the last option for
(c) transformed cells (d) None of these contraception? [2]
Ans :
Ans : (c) transformed cells
Surgical methods or sterilisation techniques
Section B are generally opted by the male or female
partners as a terminal method to prevent any
6. A lake near a village is suffering from heavy
further pregnancies. This method is used as a
mortality of fishes within the last few days.
last option for contraception because
Consider the following reasons for this. [2]
(i) it is almost irreversible.
I. Lots of urea and phosphate fertilisers
(ii) it involves surgical procedure which
were used in the crops in the vicinity.
many are unwilling to undergo.
II. The area was sprayed with DDT by an
aircraft. 9. A haploid organism produces gametes by
III. The lake water turned green and stinky. mitosis. Does that mean, meiosis never occurs
IV. Phytoplankton populations in the in such organisms? Explain. [2]
lake declined initially thereby greatly Ans :
reducing photosynthesis. In haploid organisms, gametes are produced
Which of the above given reasons can be by mitotic division. In such organisms,
reconciled together to explain the main cause meiosis occurs during the development of
of fish mortality in the lake? zygote. It is the only diploid stage (formed by
Ans : the fusion of two haploid gametes) in the life
Statements I and III are the most probable cycle of a haploid organism that can undergo
reasons of fish mortality in the lake. Due to meiosis. Thus, meiosis may also occur in such
continuous addition of phosphate, nitrate organisms.
and urea rich agricultural run offs, the lake or
become nutrient enriched. Such water bodies Human placenta acts as a structural and
are highly productive and support a dense functional unit between the foetus and
growth of plants and algae. The extensive maternal body. Explain the statement by
giving any
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Ans : individual’s body becomes prone to most


Human placenta acts as a structural and infectious diseases.
functional unit between the foetus and
maternal body. It forms an intimate vascular Section C
connection between them and performs
many important functions. Some of them are 13. Students while performing the experiment
as follows were given two different vectors for two
(i) It facilitates the supply of oxygen and different bacterial colonies that were cultured
nutrients to the embryo. in a chromogenic substrate. They observed
(ii) It secretes hormones that are necessary that the bacterial colonies with cloning vector
to maintain pregnancy, e.g. hCG, hPL, A were colourless while those with cloning
oestrogen, progesterone, etc. vector ‘B’ were blue in colour. Explain the
phenomenon and the procedure behind the
10. How are sticky ends formed on a DNA strand change in colour. [3]
? Why are they so called? [2] Ans :
Ans :
Students were working to differentiate
Restriction enzymes cut the strands of the recombinants and non-recombinants on the
DNA, a little away from the centre of the basis of their ability to produce colour in the
palindromic sites, but between the same presence of a chromogenic substrate.
two bases on opposite strands. This leaves During this method, a recombinant
sticky single-stranded position at the ends. DNA is inserted in the coding sequence of
These overhanging stretches are called sticky an enzyme 3-galactosidase. This results in
ends. These are named so because they form the inactivation of the enzyme (insertional
hydrogen bonds with their complementary inactivation). If the plasmid in the bacterium
cut counterparts. does not have an insert, it gives blue
11. Suggest why only about 1% of the genetic coloured B colonies. The presence of insert
information gets transcribed into functional causes insertional inactivation and therefore,
RNA sequences in most of the mammalian colourless A colonies are produced. These are
cells. [2] marked as recombinant colonies.
Ans : or
Only about 1% of genetic information is A mixture of fragmented DNA was
transcribed into functional RNA sequences electrophoresed in an agarose gel. After
because most of the DNA is ‘junk’, i.e. the staining the agarose gel with ethidium
DNA which does not code for any protein or bromide, no DNA bands were observed.
RNA. Also, some sequences of DNA bases What could be the reason?
only code as non-sense codes, while some Ans :
are introns that code for certain unused
After staining the agarose gel with ethidium
sequences of mRNA. As only some genes get
bromide, no DNA bands were observed
switched on in a cell, the amount of DNA
during electrophoresis, it could be due to the
involved is very little.
following reasons
12. Due to improper functioning, thymus gland (i) DNA sample that was loaded on the
was removed from the body of a 15-year-old gel may have got contaminated with
person. Can you predict the effects that will nuclease (exo or endo both) so that it
be observed in the immune functions of this got completely degraded.
individual? [2] (ii) Electrodes were put in such an
Ans : orientation in the gel assembly that
Thymus is the primary lymphoid organ. anode is towards the wells (where
In thymus gland, immature lymphocytes DNA sample is loaded). Since, DNA
differentiate into antigen-sensitive molecules are negatively charged they
lymphocytes. If thymus gland is removed move towards anode and hence, move
from the body of a person, his immune out of the gel instead of moving into the
systemhere
will to
become weak. Educart
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(iii) Ethidium bromide was not added present. The quantity of human skin pigment
at all or was not added in sufficient melanin determines human skin colour which
concentration and so, DNA was not depends upon the presence of number of
visible. dominant alleles. So, the human skin colour
14. A non-haemophilic couple was informed shows many gradations.
by doctor that there is a possibility of a 15. (i) List any two conditions on which the
haemophilic child to be born to them. Suggest development of a diploid cell depends.
and substantiate the basis on which doctor (ii) If the number of chromosomes present
conveyed this information. Also, identify the in the meiocyte of a plant is 24, then
genotype of parents. [3] workout the number of chromosomes in
Ans : the gamete and zygote of this plant. [3]
The doctor conveyed this information on the Ans :
basis of pedigree analysis. It is a strong tool (i) Zygote, a diploid cell is formed as a
through which inheritance of a specific trait, result of fusion of male and female
abnormality or disease can be traced. gametes. Its development depends on
Since, both the parents are non- the following two main conditions
haemophilic, the genotype of both will be XY (a) Type of life cycle of the organism.
(father) and XXh (mother, carrier of disease). (b) Environment to which it is exposed.
Their probability of having a diseased child (ii) If the number of chromosomes present in
can be predicted with the help of the following meiocyte (2n) is 24, then the number of
cross chromosomes in gamete will be (n)=12
and number of chromosomes in zygote
(2n) will be 24.
16. Refer to the diagram given below and answer
the questions that follows. [3]

Thus, there is 25% chance of having a


haemophilic child (male).
or
In pea plants, the colour of the flower is
either violet or white, whereas human skin (i) Label the parts A and B.
colour shows many gradations. Explain (ii) Which parts of flower develop into seed
giving reason, how it is possible. and fruit?
Ans : (iii) What is the function of both the labelled
parts?
In pea plants, the colour of the flower is either
Ans :
violet or white because this trait is controlled
by a single gene having two alleles, one of (i) The part A is seed and B is pericarp.
which (violet) is dominant over the other (ii) The parts of flower which develop into
(white). In the presence of dominant allele, seed and fruit are ovule and ovary,
the flowers will be of violet colour and in the respectively.
presence of both recessive alleles, flowers will (iii) The seed germinate to produce new
be of white colour. plants while pericarp is protective in
Whereas, human skin colour is the type function. It protects the young seed.
of inheritance that is controlled by one or 17. (i) Female reproductive organs and associated
more genes, in which dominant alleles have functions are given below in column I and
cumulative effect. Each dominant allele column II. Fill in the blanks. [3]
expresses a part of the trait and the full trait
is shown only when all dominant alleles are
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Column I Column II 19. Entamoeba histolytica is a protozoan


parasite which causes amoebic dysentery
Ovaries Ovulation in man. This parasite occurs worldwide
Oviduct (i) wherever sanitation and hygiene are poor.
(ii) Pregnancy Comment on the transmission of this
parasite from its reservoir to a host. Also,
Vagina (iii)
identify the symptoms of this disease which
(ii) State the function of the following will be displayed by an affected individual.
(a) Acrosome What will be your advice for measures to be
(b) Sperm tail adopted for prevention of this disease? [3]
Ans : Ans :
Transmission: The disease causing parasite
Column I Column II
is transmitted by mechanical vectors like
Ovaries Ovulation houseflies, cockroaches, etc., from the faeces
Oviduct Fertilisation of the infected person (reservoir) to the food
Uterus Pregnancy and contaminate them.
Symptoms: The patient passes out blood
Vagina Birth and mucus with stools. He also experiences
(ii) (a)Acrosome It contains hydrolytic griping pain in the abdomen (cramps) along
enzymes required to dissolve with fever, constipation, etc.
the membranes of the ovum for Preventive measures
fertilisation. (i) Maintain cleanliness in public and at
(b) Sperm tail It helps in the movement personal level.
of sperm in the female genital tract (ii) Practice hygiene like washing hands
for fertilisation. before eating, keeping food covered, etc.
(iii) Proper medication or vaccination should
18. When Ankit was diagnosed with AIDS, his be taken for treatment and prophylaxis.
colleagues wanted him to leave the workplace
due to the fear of spread of AIDS. However, 20. Appraise yourself with the measures that
his boss was against their decision. He called Indian government has taken to control the
a counsellor to setup a meeting with people vehicular air pollution in Delhi and other cities
to clear their misconceptions. [3] of India such as introduction of CNG and
other alternate fuels, EURO norms, emission
(i) Give the expanded form and causative regulations, etc. Can you compile in your
agent of AIDS. own words, some of the positive outcomes of
(ii) How does transmission of AIDS occur? these regulations on the environment? [3]
(iii) According to your view, demand of
Ans :
Ankit’s colleagues is valid or not?
Provide plausible reasons for the same. Measures to control air pollution and their
Ans : positive outcomes are as follows
(i) Switching over the public transport
(i) AIDS is Acquired Immuno Deficiency and other vehicles to CNG. Since,
Syndrome. It is caused by Human CNG burns efficiently without leaving
Immunodeficiency Virus (HIV). unburnt residues behind, it resulted
(ii) HIV gets transmitted by sexual contact in reduced use of diesel and petrol,
with infected person, by sharing of decreased level of vehicular exhausts
infected needles, by transfusion of containing harmful gases.
contaminated blood and from infected (ii) Use of catalytic converters, electrostatic
mother to her child through placenta. precipitators and other control devices
(iii) No, demand of Ankit’s colleagues is in vehicles. Automobiles, thermal
not valid. AIDS does not spread by power plants, etc., are major causes
handshake, hugs, sharing of food and of atmospheric pollution. Introducing
other articles. catalytic converters in vehicles reduces
the emissions of poisonous gases like
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CO, NO2, etc. Electrostatic precipitators Ans :


help in controlling particulate matters (i) The given pyramid is an inverted
emitted from thermal power plants. ecological pyramid. The base and
(iii) Compulsory and regular check-up the apex of this pyramid producers
of pollution emission of vehicles and (first trophic level) and tertiary level
enforcement of Euro norms (which consumers, respectively.
specify emission levels in vehicles). As a (ii) Examples of such ecological pyramids
result of these changes, the level of air are as follows
pollution in Delhi NCR have reduced (a) Pyramid of number in a tree
considerably. ecosystem.
21. New species are evolving in a short time (b) Pyramid of biomass in a pond
scale of months or few years rather than the ecosystem
million years due to increasing anthropogenic 23. Study the figure given below and answer the
actions. Create a situation using an example following questions. [3]
to predict the outcome of these actions on
the evolutionary process. [3]
Ans :
Human activities, i.e. anthropogenic actions
have enhanced evolution. As a result, new
species of living beings are evolving in a very
short span of time. For example, excessive
or overuse of DDT as a fertiliser in crops
resulted in evolution of DDT resistant (i) Identify the molecule ‘X’ synthesised
mosquitoes. When DDT was used first time, by ‘i’ gene. How does this molecule get
many mosquitoes died, but a few survived. inactivated?
These mosquitoes showed resistance and (ii) Identify the structural gene that codes
reproduced in the presence of DDT. Offspring for β -galactosidase.
produced by these mosquitoes evolved as a (iii) When does the transcription of this
new species which were increasingly resistant gene get stopped?
to DDT. Hence, DDT as a pesticide was not Ans :
effective on newly evolved mosquito
(i) Molecule ‘X’ synthesised by ‘I’ gene is a
population.
repressor protein. It gets inactivated on
combining with an inducer molecule.
Section D (ii) ‘z’ gene codes for β -galactosidase.
(iii) Transcription of gene stops in the
22. The diagrammatic illustration of connection
following conditions
between different trophic levels is given
(a) When substrate lactose is not
alongside. [3]
available.
(b) When energy source, i.e. glucose is
available to the cells.
24.

(i) Identify the type of ecological pyramid


and explain what do the base and the
apex of this pyramid indicate?
(ii) Give examples of such type of ecological
pyramids with respect to number and With reference to the above diagram of E.coli
biomass. cloning vector pBR322, answer the following
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questions. [3] • Death may also occur in serious cases


(i) In E.coli cloning vector pBR322, a foreign Pneumonia: It is caused by the bacterium
gene segment is to be introduced into Streptococcus pneumoniae and Haemophilus
the ampR region. From the restriction influenzae. It infects alveoli of lungs.
enzymes given below, which one you Way of entry: It enters the body by inhaling
would probably use and why? Barn H1, the droplets released by an infected person
Sal I, Pvu II, Eco RI. and also by sharing glasses and utensils with
(ii) Highlight the reasons for why could an infected person.
not you choose the other remaining Symptoms
restriction enzymes for your work. • It includes fever, chills, cough and
(iii) How will the insertion of this enzyme headache.
aid in RDT process? • In severe cases, lips and finger nail may
Ans : turn greyish to bluish in colour.
(ii) Chikungunya is a viral disease. Its
(i) Restriction enzyme Eco RI would be
causative agent is chikungunya virus.
used, because the restriction site for this
It is transmitted by the bite of Aedes
enzyme is present in our target site, i.e.
aegyptii.
ampR region.
(ii) Other enzymes like Barn H1, Sal I and or
Pvu II cannot be used because their What is an antibody? Give its structure and
restriction site is not present in ampR mention its different types.
region but in tetR region. Ans :
(iii) The insertion of foreign DNA fragment
will inactivate the ampicillin resistance An antibody is a type of protein molecule
gene in the vector. As a result, the produced by the B-lymphocytes in response
recombinant plasmid will become to exposure to an antigen. It has four peptide
ampicillin sensitive. chains. Out of these chains, the two smaller
ones are called as Light (L) chains and the
two longer ones are called as Heavy (H)
Section E chains. So, it is designated as H2L2. Antibody-
25. (i) Typhoid and pneumonia are bacterial mediated response is called as humoral
diseases that infect human beings. Mention immune response.
their causative agents and symptoms. The structure of an antibody molecule is
Also, mention the way in which both the as follows
pathogens infect the human body. [5]
(ii) Give one viral disease and also mention
its causative agent and vector.
Ans :
(i) Typhoid and pneumonia are bacterial
diseases that infect human beings.
Typhoid : It is a disease caused by bacterium,
Salmonella typhi.
Way of entry: It enters the small intestine
through contaminated water and food and
migrates to other organs of the body through
blood. Figure: Structure of an Antibody
Symptoms: Its symptoms include
The different types of antibodies produced
• High fever (39-40°C)
in the body are IgG, IgM, IgA, IgE and
• Weakness
IgD. IgG protects the developing foetus
• Stomach pain
by providing natural passive immunity.
• Constipation
IgM provides immune response to complex
• Headache
organisms like virus and bacteria. IgA is a
• Loss of appetite
component of colostrum and provides passive
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immunity. IgE acts as a mediator in allergic Ans :


response and IgD activates B-cells to secrete Haemophilia, a blood clotting disorder is
antibodies. majorly transmitted from an unaffected
26. A single mutant allele is responsible for the carrier female to 50% of the male offspring.
abnormal form of haemoglobin (i.e. HbS ). If The gene for haemophilia is located on the
it is present in a homozygous state (HbS HbS ), X-chromosome. The possibility of female
person develops sickle-cell anaemia, but if the becoming haemophilic is extremely rare.
allele is found in heterozygous state (HbAHbS ), Condition I
the person only shows few symptoms for the
disease. On the basis of the above situation,
work out a cross for a condition in which a
woman, who is homozygous for allele marries
a male who is heterozygous for the allele.
Assess the probability of having a normal or
a diseased child for each of her pregnancies.
Condition II
Also, analyse the advantage that is conferred
by the heterozygous condition of gene HbS. [5]
Ans :
Cross between homozygous female and
heterozygous male is as follows

This indicates that for a female to become


haemophilic, the gene should be in a
homozygous condition, i.e. mother of such
female should be carrier and father should be
From the cross, it is clear that 50% offspring are haemophilic.
homozygous for the HbS allele, whereas 50% are Colour blindness is the inability to
heterozygous for both HbS and HbA alleles. distinguish among some or all colours. In this
Above cross shows that a child has equal disorder, mutant forms of genes change the
probability of developing into a diseased light absorbing capacity of sensory receptors
child or a carrier with some symptoms of the inside the eyes. This trait is mainly common
disease. Therefore, the woman has 50% risk in men, but heterozygous woman may also
of giving birth to a diseased child, everytime show symptoms and pass the disorder to
she conceives. some of her sons.
The person with heterozygous condition Condition I
(HbAHbS) for sickle-cell anaemia does not
develop sickle-cell anaemia and malaria,
Due to abnormal shape of haemoglobin, the
malarial parasite is not able to survive and
complete its life cycle.
Hence, in this case, the heterozygous
individuals have more chances of survival,
which is an advantage with respect to the Condition I
homozygous individuals.
or
Haemophilia and colour blindness are sex-
linked recessive disorders determined by the
alteration or mutation in the single gene.
Evaluate the pattern of inheritance of both
disorders in human beings with the help of This cross shows that female can be
a cross. Also, predict the type of inheritance colourblind only in homozygous condition.
shown by these disorders. Both of these disorders show criss-cross
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inheritance, i.e. transmission of disease occurs (d) Expansion of agriculture: In the last
from father to daughters and from mother to few years, due to the expansion of
her sons. agriculture, land available for natural
27. Given below is the type of global biodiversity vegetation is very less. It is also a
representing the proportionate number of major cause for the less number of
species of major taxa of plants. Observe ferns and mosses.
it carefully and answer the questions that (iii) Angiosperms are the most advanced
follows. [5] and algae are the most primitive group
of plants in the given biodiversity.
(iv) Fungi are heterotrophic in nature due
to lack of chlorophyll. They are able to
sustain themselves as a large population
because of their ability to reproduce
both asexually and sexually.
or
Figure: Representation of Global Biodiver- With the help of a flowchart, explain what
sity of Major Taxa of Plants are the various major approaches to conserve
(i) Identify the most endangered group of biodiversity.
plants among all categories. Ans :
(ii) What may be the reason behind such The major approaches to conserve biodiversity
less population of mosses and ferns? can be ex situ or in situ. These are given in
(iii) Name the most advanced and the the following flowchart
most primitive group of plants in the
biodiversity given above.
(iv) Fungi, inspite of being heterotroph are
able to sustain themselves as a large
population. Explain.
Ans :
(i) Lichens are the most endangered plants In situ Conservation
among the given categories. It involves the protection of species in their
(ii) Population of mosses and ferns is less natural habitats.
may be due to the following reasons (i) Biodiversity hotspots are the regions of
(a) Habitat loss: Destruction of habitat high levels of species richness and high
(i.e. moist and shady environment degree of endemism (that is species
as in forests) is the most important confined to that region and not found
cause of extinction of mosses anywhere else).
and ferns. It could be due to The total number of biodiversity
urbanisation. hotspots is 34. Three hotspots, which
(b) Overexploitation: Humans are cover India are Western Ghats/Sri
always dependent on nature for food Lanka, Indo-Burma region and Eastern
and shelter. However, when human Himalayas.
needs turn into greed, it leads to (ii) Protected areas are ecologically and
the overexploitation of natural biodiversity rich regions. India has 18
resources. Many species of mosses Biosphere Reserves, 90 National Parks
and ferns are also used for various and 449 Wildlife Sanctuaries. The first
purposes. Their overexploitation has National Park setup in India was Jim
led to a decrease in their number. Corbett National Park.
(c) Environmental pollution: Many Ex situ Conservation
ferns and mosses cannot grow in It involves the conservation of selected species
very polluted environment. Thus, outside their natural habitats.
environmental pollution is also (i) Protection of gametes can be done by
responsible for their less number. storing material in the places where
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stocks remain viable for a longer period


of time without harming their genetic
variability.
(ii) Protection of threatened species comes
under the category of off-site collections
of various wild and domesticated
species. India has around 800 parks
where animals which have become
extinct in wild are maintained and
has around 1500 botanical gardens to
collect, cultivate and display different
varieties of plant

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