SUMMARY

Dyslipidemia is a lipid metabolism disorder known as one of the risk factors for coronary
heart disease, alongside other risk factors such as hypertension and diabetes mellitus.
Dyslipidemia is characterized by an increase or decrease in lipid levels in the plasma. The
primary lipid abnormalities include an elevation in total cholesterol (TC), triglycerides (TG),
low-density lipoprotein (LDL), and a decrease in high-density lipoprotein (HDL). Elevated
triglyceride levels (TG) can result from the consumption of high-fat foods.

The consumption of high-fat diets, including a mix of duck egg yolks, quail egg yolks,
and palm oil, can lead to the occurrence of dyslipidemia. This condition can be treated
pharmacologically and non-pharmacologically. Pharmacological therapy, such as statins, has
been proven to lower TG levels. In Indonesia, alternative treatments like herbal therapy are
commonly used by the population. Celery leaves are abundant in Indonesia and contain active
compounds such as flavonoids, saponins, tannins, and phytosterols. Several studies have
explored the effects of ethanol extract from celery leaves on reducing TG levels in dyslipidemia
conditions. Researchers attribute the effects to active compounds that inhibit fat absorption in the
intestines and trigger lipoprotein lipase (LPL) in fat metabolism.

This study aims to determine the preventive effects of ethanol extract from celery leaves
on the increase of TG levels in Wistar rats induced with a high-fat diet. The research model
employs a Randomized Controlled Trial (RCT) with a post-test only control group design. Forty-
two experimental animals will be divided into six groups: Group I (normal control) with a
standard diet, Group II (negative control) with a high-fat diet, Group III (positive control) with
simvastatin and a high-fat diet, Group IV with celery leaf ethanol extract at 125mg/kgBW and a
high-fat diet, Group V with celery leaf ethanol extract at 250mg/kgBW and a high-fat diet, and
Group VI with celery leaf ethanol extract at 400mg/kgBW and a high-fat diet. The study will be
conducted for 35 days, including a 7-day adaptation period, 28 days of treatment, and euthanasia
on day 36 for TG level examination using intracardiac blood, with TG levels calculated using
spectrophotometric methods.

TG calculation data will be analyzed using Statistical Product and Service Solution
(SPSS). Normality tests will indicate normal distribution if (p>0.05), and homogeneity tests will
assess the variance between two or more different data sets with a significant value (p>0.05). If
the groups are normally distributed and homogeneous, a One-way ANOVA test will be
performed, considering significance values (p<0.05), followed by post-hoc LSD tests with
significance values (p<0.05). The mean results show a decrease in TG levels in Groups IV, V,
and VI compared to Group II. This indicates that ethanol extract from celery leaves can prevent
the increase of TG levels in rats induced with a high-fat diet.