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Current Traditional Medicine, 2023, 9, e270622206405


REVIEW ARTICLE
ISSN: 2215-0838
eISSN: 2215-0846

Therapeutic Benefits of Miracle Tree Moringa oleifera: A Complete Overview

Faridi Shabnam1,*, Isha Rani2,*, Dhiman Vivek1, Rajat Goyal1,2 and Rupesh Kumar Gautam1
1
MM School of Pharmacy, MM University, Sadopur (Ambala), Haryana, 134007, India; 2MM College of Pharmacy,
MM (Deemed to be University), Mullana (Ambala), Haryana, 133203, India

Abstract: Generally, Ayurvedic or herbal products are considered safe to use. According to a re-
ARTICLE HISTORY
port, more than 50% of the global population depends upon herbal products. To meet the global de-
mand for ayurvedic products, more and more herbal plants are analyzed for their active constituents
Received: January 18, 2022 and health benefits. Moringa oleifera is one such plant that is analyzed for its therapeutic benefits.
Revised: April 09, 2022
Accepted: April 19, 2022 Various parts of this plant, such as the leaves, roots, seed, bark, fruit, flowers, and immature pods,
act as cardiac and circulatory stimulants with antipyretic, antitumor, anti-inflammatory, antiepilep-
DOI: tic, diuretic, antiulcer, antispasmodic, antihypertensive, cholesterol-lowering, antidiabetic, antioxi-
10.2174/2215083808666220627121322
dant, antibacterial, hepatoprotective, and antifungal activities. This review is about the chemical
Current Traditional Medicine

composition of this tree’s market preparation and its different applications.

Keywords: Moringa oleifera, miracle tree, horseradish tree, phenolic compounds, antioxidant, anticancer.

1. INTRODUCTION Carlquist suggested the same based on wood anatomy [9].


Following Fig. (2) describes the taxonomical classification
Moringa oleifera, belonging to the family Moringaceae, of Moringa oleifera.
also called ‘drumstick tree’ or ‘horseradish tree’, is a peren- Each part of this plant is of higher nutritive value. Alka-
nial tree belonging to tropical and subtropical regions of the loids, tannins, steroids, phenolic acids, saponins, flavonoids,
world [1, 2]. The Moringaceae family has a total of 13 spe- terpenes, etc., are the various phytochemicals present in this
cies, such as Moringa oleifera, Moringa arborea, Moringa plant [10]. The leaves of this tree are loaded with minerals
longituba, Moringa borziana, Moringa pygmaea, Moringa and vitamins and can be used to treat malnutrition and pro-
hildebrandtii, Moringa drouhardii, Moringa peregrine, mote lactation in mothers [11]. Moringa seeds consist of low
Moringa stenopetala, etc. Out of all these, M. oleifera is the molecular weight cationic proteins, which are useful in in-
most utilized and cultivated species. M. oleifera can grows dustrial water purification and hardness removal. Moringa
and survives in harsh climatic conditions [3, 4]. The plant is seed floor can be used to clarify water [12, 13] and is also
referred to as the “miracle tree” because of the enormous used as a food supplement for humans and livestock feed. It
positive impact it has on people’s livelihoods. It is reported is also called a miracle tree because of its ability to treat var-
that every plant part (seeds, flowers, stems, leaves, and roots) ious disorders like diabetes, cancer, dementia, stress, arthri-
is a great source of nutrients and produces major essential tis, etc., without causing any side effects. It can also be used
medicinal principles, curing a range of diseases. This tree to treat several psychiatric and autoimmune disorders [14].
may also serve as a nutritional and therapeutic substitute due
to its high nutritional contents and various medicinal charac- Moringa leaf is totally loaded with full of nutrients;
teristics [5]. It is cultivated all across the world as it can therefore, we can use it as an alternative to imported food
grow under severe drought and mild frost conditions. North- supplies to treat malnutrition in poor countries. It should be
west India is among the major producer of this tree [6]. The consumed either fresh or dry. Dried leaves can be stored for
Oleifera species of the Moringa tree are depicted in Fig. (1). a long time without loss of nutritional value and can be used
regularly. Many companies across the world manufacture
Out of all the 13 Moringa species, Moringa oleifera is various products of Moringa leaves, such as Moringa Tea,
the only genus in the family Moringaceae [7]. The word Moringa Tablets, Moringa Capsules, Moringa leaf Powder,
“Moringa” is derived from Southern Indian (Malayalam) Moringa Soaps, and Moringa face wash. Some beverages are
word 'Muringo' [8]. Olson, in 2002, suggested grouping of also available in the market prepared by Moringa leaves.
Moringa species based on the use of DNA sequences, while Thus, it is necessary to hygienically dry and process Moringa
leaves for further use [15].
*Address correspondence to these authors at the MM College of Pharmacy,
1.1. Moringa oleifera: Growth Characteristics
MM (Deemed to be University), Mullana (Ambala), Haryana, 133203,
India; Tel: 82788 23717; E-mail: isha12th@gmail.com and M. oleifera can grow and survive in harsh climatic condi-
MM School of Pharmacy, MMU- Sadopur (Ambala), Haryana, 134007,
India; E-mail: shabnam@mmambala.org
tions. It is cultivated all across the world as it can grow under
severe drought and mild frost conditions [4]. The optimum

2215-08/23 $65.00+.00 © 2023 Bentham Science Publishers

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Therapeutic Benefits of Miracle Tree Moringa oleifera e270622206405 Current Traditional Medicine, 2023, Vol. 9, No. 2

Fig. (1). Oleifera species of the Moringa tree.

growth requirements of Moringa oleifera [16, 17] are depict- trees can also be cultivated [16]. Tree cuttings with 4-5cm
ed in Table 1. diameter and 1m length can be cultivated. But this method
has a drawback; plants grown with this method have a much
weaker root system. Such plants are also affected by drought
and winds. The nutritional composition of Moringa also var-
ies according to the geographical area in which it grows. For
example, Indian Moringa is slightly different in nutritional
composition as compared to Moringa grown in Nigeria. In
the hot-humid season, Vitamin A was predominant, but in
the cold-dry season, Vitamin C and iron were the predomi-
nant phytochemicals present [17, 18].
Soil is also one of the major factors that affect the nutri-
tional composition of Moringa. For example, studies re-
vealed that the plants grown under poultry manure are found
to possess high phosphorus, potassium, sodium, and manga-
nese compared to those grown using fertilizer alone. Plants
grown using fertilizers alone have a different nutritional
composition compared to the one to which fertilizers are
applied in combination [19].
1.2. Moringa oleifera: Essential Phytochemicals
The essential phytochemicals of Moringa oleifera [18]
are described in Table 2.
Fig. (2). Taxonomical classification of Moringa oleifera.
Table 2. Essential phytochemicals possessed by Moringa spe-
Table 1. Optimum growth requirements of Moringa oleifera. cies.

Region Tropical/Sub-tropical Active


Parts Used Nutritional Value
Constituents
0 0
Temperature 25 C- 35 C
Dried Leaves Vitamin A 10 times more than in carrots
Soil Sandy/ loamy soil Dried Leaves Calcium 17 times more than in milk

Dried Leaves Protein 9 times more than in yogurt


pH Slightly alkaline
Dried Leaves Potassium 15 times more than in bananas
Rainfall 250mm- 300mm
Dried Leaves Iron 25 times more than in spinach

Dried Leaves Vitamin C 7 times more than in oranges


Moringa seeds are directly sown in sandy/ loamy soil
with slightly alkaline pH at 2cm depth and are allowed to
germinate for 5- 12 days [14]. Moringa can also be propagat- 1.3. Moringa oleifera: Chemical Composition
ed from saplings and placed inside a plastic bag. Saplings are
allowed to grow to about 30 cm in height and are then trans- The chemical composition of Moringa oleifera [18] is
planted into the soil [18]. But transplantation should be done described in Table 3.
with extreme care without damaging the taproots. Moringa
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Table 3. Chemical composition of Moringa oleifera.

S. No. Category Chemical constituents References

1 Flavanoids Rutin, Quercetin, Rhamnetin, Kaempferol, Apigenin, Myricetin, etc. [20-23]

2 Glucosinolates [4-O-(α-L-rhamnopyranosyloxy)-benzyl glucosinolate], 4-[(α-L-rhamnosyloxy) benzyl] isothio- [24-26]


cyanate (GMG-ITC), 4-O-(α-L-rhamnopyranosyloxy)-benzyl glucosinolate, etc.

3 Phenolics Gallic acid, Ellagic acid, Ferulic acid, Caffeic acid, o-coumaric acid, Chlorogenic acid, Gentisic [27-30]
acid, Syringic acid, ρ-coumaric acid, Sinapic acid, etc.

4 Terpenes Lutein, E-luteoxanthin, 13-Z-lutein, 15-Z-β-carotene, E-zeaxanthin, Lupeol acetate, β-amyrin, α- [31-33]
amyrin, etc.

5 Alkaloids Marumoside A and B, Pyrrolemarumine-4″-O-α-L-rhamnopyranoside etc. [34, 35]

6 Sterols β-sitosterol-3-O-β-D-galactopyranoside, β-sitosterol, Campesterol, Stigmasterol, etc. [36, 37]

Fig. (3). Chemical structures of chemical constituents (Capmesterol, Stigmasterol, B-sitosterol, and Marumoside A).

The chemical structures of these chemical constituents ment of anemia, an effective substitute for iron tablets [Beef
are described in Figs. (3-6). (2 mg Iron) < Moringa leaf powder (28 mg Iron)] [39].
Moringa leaves can provide 25.5 to 31.03 mg of zinc per kg
1.4. Moringa oleifera: Nutritional Characteristics [40]. It can fulfill the daily requirement of zinc, which is
essential for the production of sperm and the synthesis of
1.4.1. Leaves DNA and RNA. Moringa leaves also have a low calorific
The leaves of this tree are loaded with minerals and vita- value and can be used in the diet of obese patients [41].
mins and can be used to treat malnutrition and promote lacta- Leaves of Moringa are generally used in many countries as
tion in mothers. Moringa leaves are rich in vitamins, miner- vegetables. The addition of Moringa leaves to cow feed in-
als, PUFA, saturated fatty acids, and antioxidants [18, 19]. creases milk production by about 43%-65% [42]. The pres-
Moringa leaves consist of minerals (calcium, potassium, ence of phytosterols in leaves can promote the activity of
zinc, magnesium, iron, and copper), vitamins (beta-carotene mammary glands [43].
of vitamin A, vitamin B, such as folic acid, pyridoxine, and 1.4.2. Pods
nicotinic acid, vitamin C, D, and E), PUFA (omega-3 and
omega-6), tannins, sterols, terpenoids, flavonoids (quercetin, Moringa pods consist of 46.78% fiber, 20.66% protein,
glucosides, rutinosides, and malonyl glucosides), saponins, 30% amino acids, palmitic acid, linoleic acid, and oleic acid.
anthraquinones, alkaloids, reducing sugar and glucosinolates β-carotene and sterols in the plant pods are potent inhibitors
[37]. Moringa leaves are loaded with calcium [8 ounces of of ROS (reactive oxygen species) intermediates and thus
milk (300-400 mg calcium) < Moringa leaves (1000 mg Cal- help in preventing cancer. These constituents further reduce
cium)] [38]. Moringa leaf powder can be used for the treat- the plasma cholesterol level and enhance the fecal excretion
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Fig. (4). Chemical structures of chemical constituents (Quercetin, Apigenin, Rutin, Kaempferol, Rhamnetin, and Myricitine).

Fig. (5). Chemical structures of chemical constituents (Alfa Amyrin, Beta Amyrin, and 15-Z B Carotene).
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Fig. (6). Chemical structures of chemical constituents (Gallic Acid, Ellagic acid, Syringic acid, Ferulic acid, p-Coumaric acid, Sinapinic acid,
and Caffeic acid).

of the same. Moringa pods can be used in the treatment of antifungal activity (against Neurospora crassa and Aspergil-
colon cancer and digestive problems [44, 45]. lusniger). Moringa bark can also be used to treat UTIs [53,
54].
1.4.3. Seeds
Moringa seed oil consists of linoleic acid, oleic acid, and 1.5. Moringa oleifera: Therapeutic characteristics
76 % PUFA (Polyunsaturated fatty acids). Moringa seed oil 1.5.1. Anti-diabetic
is a good substitute for olive oil and is used for the control of
cholesterol in the body. Moringa seed oil can be extracted by Moringa can act upon both Type 1 and Type 2 diabetes.
cold pressing technique. The oil comprises mainly behenic Studies revealed that the aqueous extract of Moringa can be
acid, which makes this oil more resistant to rancidity. used to treat STZ- induced type 1 diabetes and type 2 diabe-
Moringa seeds also possess anti-diabetic activity. 50- tes in rats. In the case of STZ- induced diabetes, STZ causes
100mg/kg dose of Moringa seed powder in rats act by reduc- dephosphorylation of ATP, which in turn promotes the syn-
ing the blood glucose level. Moringa seeds also show anti- thesis of superoxide and ROS (Reactive Oxygen Species).
arthritic activity due to their anti-inflammatory property. The This then leads to apoptosis of beta-pancreatic cells, which
seeds also can purify water through the precipitation of results in reduced secretion of insulin. This is the main cause
heavy metals. Seeds also show antimicrobial activity due to of diabetes [55]. Moringa seed powder is also known to re-
the presence of constituents like pterygospermin and benzyl duce the blood glucose levels [56, 57]. Studies revealed that
isothiocyanate [46-48]. the administration of 500mg/kg of Moringa seed powder in
rats stimulates the antioxidant enzymes, which further can
1.4.4. Flowers bring down the level of superoxide and ROS [58]. Thus, can
Moringa flowers can be used commercially to produce be used to treat diabetes. One extract from the moringa leaf
honey as these are a good source of nectar. The flowers also has been shown that it is effective in lowering blood sugar
possess anti-cancer properties that act by inhibiting the levels within 3hrs after ingestion, though less effective than
growth of PC3 cells [49]. Therapeutic activities exerted by the standard hypoglycemic drug, glibenclamide. Kumari et
Moringa flowers are antimicrobial (active against bacterial, al. published a study in 2010. For 40 days, type 2 diabetes
parasitic, and viral infection), antiulcer (due to the presence participants were given 8 g of dried M. oleifera leaf in tablet
of alkaloids, saponins, and tannins), antipyretic, anti- form. The study enlisted the participation of 46 subjects. The
psychiatric (nervous disorders such as hysteria and demen- fasting and postprandial blood glucose levels were 28 per-
tia), anticancer, diuretic, and hepatoprotective [50-52]. cent and 26 percent less, respectively, at the end of the trial.
Additionally, compared to the control group, total cholester-
1.4.5. Bark ol, triglycerides, LDL cholesterol, and very low density lipo-
Moringa bark extract shows antibacterial (Staphylococ- protein cholesterol were 14 percent, 14 percent, 29 percent,
cus aureus, Citrobacter freundii, Bacillus megaterium) and and 15 percent reduced, respectively [59].
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1.5.2. Anticancer tion, reduces cell viability, causes oxidative damage, and
inactivates the PI3K/Akt/Foxo1 pathway, which inhibits
Cancer is the uncoordinated, uncontrolled, and prolifera-
osteogenic induction in rat BMSCs. By restoring cell prolif-
tive growth of tumor cells. Over 24 lac cancer patients are
eration and viability, lowering peroxidative damage, block-
diagnosed every year in India [60]. Yet, there is not a specif-
ing apoptosis, and activating the PI3K/Akt/Foxo1 pathway,
ic cause for this deadly disease. But some factors are known serum-containing Moringa oleifera leaf extracts positively
to promote cancer. For example, smoking, tobacco, radiation
regulated bone marrow mesenchymal stem cells (BMSC)
exposure, and too much sunlight exposure. Treatment of
osteogenic development. These findings support the thera-
cancer includes surgery, chemotherapy, radiotherapy, and
peutic use of Moringa oleifera leaf extracts to treat patients
vaccination (only for cervical cancer). Moringa is a natural
with osteoporosis caused by ageing and peroxidative dam-
product that is known to possess anti-cancer properties.
age, and they also suggest that using Moringa oleifera leaf
Moringa can be used as a specific anti-neoproliferative agent extracts to prevent osteoporosis and reduce fragility could be
[61] that acts specifically on cancer cells. The active constit-
a promising strategy [73].
uents of the Moringa leaves responsible for anti-cancer activ-
ities are quercetin, kaempferol, glucosinolates, niazimicin, In a distinct human trial, Kushwaha et al. (2014) exam-
and benzyl isothiocyanate [62]. Because of its low toxicity ined 30 postmenopausal women who were given 7 g of M.
[63] and the presence of a range of phytochemicals [47], M. oleifera leaf powder daily for three months. A control group
oleifera has a lot of potential as a source of anticancer medi- of 30 postmenopausal women was also included. Significant
cines. Many published studies indicate encouraging results increases in serum glutathione peroxidase (18.0%), superox-
of various extracts from the moringa plant [64]. The anti- ide dismutase (10.4%), and ascorbic acid (44.4%) were
cancer efficacy of M. oleifera leaf extracts was studied in found, along with significant decreases in malondialdehyde
human hepatocellular carcinoma HepG2 cells by Jung et al. (16.3%) and lipid peroxidation (lipid peroxidation). Fur-
in 2015. It was shown that M. oleifera leaf extracts induce thermore, there was a considerable decrease in fasting blood
apoptosis in HepG2 cells. Oral dosing of the leaf extracts glucose levels (13.5%) as well as an increase in haemoglobin
considerably inhibited (upto 50 percent) the proliferation of (17.5%). There were no side effects noted [74].
HepG2 cells and A549 non-small cell lung cancer cells in the Several studies have shown that leaf extracts can de-
hollow fibre experiment. These findings corroborate the use crease liver damage caused by paracetamol. Fakurazi et al.
of soluble M. oleifera leaf extracts as orally administered (2008) found that giving 200 and 800 mg/kg of water and
medicines to treat human liver and lung malignancies [65]. ethanol extracts of M. oleifera leaves reduced acetamino-
Krishnamurthy et al. in 2015 investigated the anti-cancer phen-induced liver injury, as measured by lower AST, ALT,
properties of moringa leaf extract, n-hexane, chloroform, and ALP levels and higher hepatic glutathione levels [75].
ethyl acetate, methanol, and fractions of ethyl acetate ex- Additionally, Fakurazi et al. in 2012 revealed that i.p. admin-
tracts of M. oleifera leaves in vitro and in vivo, using Hep-2 istration of hydroethanol extracts of Moringa oleifera flow-
cell lines and Dalton's lymphoma ascites paradigm in mice, er and leaves at doses of 200 and 400 mg/kg body weight
respectively. One fraction, with a CTC50 value of 12.5 +0.5 guarded against acetaminophen-induced liver disease. The
g/ml, was found to have a possible cytotoxic effect in Hep-2 extracts reduced hepatic lipid peroxidation, improved gluta-
cell lines. When compared to the control group, in vivo trials thione levels, and boosted superoxide peroxidase and cata-
with dosages of 5 and 10 mg/kg, p.o. showed a substantial lase levels, all of which are antioxidant enzymes [76].
reduction in body weight and an increase in overall survival.
These results were likewise equivalent to those obtained in The immunomodulatory effect of Moringa was found use-
animals treated with standard, 5-fluorouracil [66]. In 2021, ful in the treatment of autoimmune disorders like rheumatoid
Xie et al. discovered that Moringa oleifera alkaloid extract arthritis. It acts by reducing the synthesis of TNF-alpha and
has potent inhibitory action against A549 cell proliferation IL-1 [77]. Moringa is also known to possess antimicrobial
and migration, as well as the ability to trigger apoptosis and activity against several microbes, including Bacillus subtilis,
cell cycle arrest in A549 cells. It was also suggested that Staphylococcus aureus, and Vibrio cholera [78]. The thera-
peutic applications of various parts of Moringa oleifera, along
alkaloid extract's effects were mediated by inhibiting the
with their active constituents, are described in Table 4.
activation of the JAK2/STAT3 signaling pathway [67].
1.5.3. Miscellaneous 1.6. Moringa oleifera: Commercial Applications

The ability of Moringa to reduce the ROS (reactive oxy- Moringa seeds contain about 40% edible oil called Ben
gen species) can be found useful in preventing cerebral is- oil [101]. This colorless and odorless oil resists rancidity as
chemia [68, 69], which is characterized by obstruction of compared to other botanical oils. After the extraction of oil
blood flow to the brain. Moringa leaf extract stimulates ace- from seeds, the seed cake left behind can also be used as a
tylcholine and other cholinergic neurotransmitter synthesis fertilizer. Ben oil has mosquito repellant properties and can
and thus can prevent dementia [70]. Inhibition of acetylcho- be used in dermal preparations [101]. Seed oil can be used to
line esterase enzyme also improves memory. A study evalu- lubricate industrial machinery [102]. It can also be used in
perfumes, paper, and textile industries. It is a good substitute
ates that the administration of Moringa in rats can reduce
for olive oil [103].
urea and creatinine levels in the blood and, thus, can be used
to prevent renal failure [71]. Moringa can also act as an anti- Chemical coagulants (aluminium sulfate, ferric sulfate)
ulcer agent [72]. Administration of 500mg of Moringa can clarify the water by neutralizing the suspended particles to
reduce gastric acidity by about 86.15%. According to a study form flocs and making them filterable. Moringa seed powder
conducted by Liu et al. in 2021, H2O2 inhibits cell prolifera-
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Table 4. Therapeutic applications of various parts of Moringa oleifera along with their active constituents.

Body Part Active Constituent Therapeutic Application References

Behenic acid Acne treatment [79]

Vitamin A, C and E Anti-wrinkle [80]


Isothiocyanates (reduces insulin resistance) Anti-diabetic [81, 82]
Leaves
Terpenoids (reduces intestinal glucose
uptake)
Phenolic compounds (increases glucose
tolerance)
Vitamin C and E (prevent hair fall) Nourishment of Hair [83, 84]
Zinc (recovers damaged hair follicles)
Arginine, Cysteine and Methionine (pro-
mote hair growth)
Omega-3 fatty acids (strengthens the hair)
Iron and Vitamin C (boosts immunity) Boosts energy and stamina [85]

Isothiocyanate and Niazimicin (prevent Anti-hypertensive [86]


thickening of arteries)
Isothiocyanates Anti-inflammatory [87]
Vitamin A, C, and E Anti-ageing [79, 80]

Vitamin C and E Anti-Alzheimer [88]


Behenic acid Anti-hyperlipidemia [89]

Pterygospermin, Morngine and Benzyl Anti-microbial [90]


isothiocyanate
Glucosinolates Anti-cancer [78]
Seeds
Anti-oxidants (prevent oxidative damage of Improves fertility [91]
sperm)
Isothiocyanates Anti-inflammatory [87]
Tryptophan Sedative [92]

Zinc Blood sugar regulation [89]


Calcium and Magnesium Cures joint pain [93]
Tannins and Phenols Antibacterial [90, 91]
Alkaloids, Saponins and Tannins Antiulcer [39]

Vitamin A, C, and E Anti-oxidant [94]


Flowers
Isothiocyanates and Thiocarbamates Hepato-protective [95]

Flavonoids Antimicrobial [90, 91]


Bark Tannins, Phenols, and Alkaloid Anti-inflammatory [87]

Peptides Anti-fungal [96]

Terpenoids and Glycosides Anti-pyretic [97]


Terpenoids and Glycosides Anti-malaria [98]

Vitamin B Cures Stomach pain [99]

Saponins Cures tooth decay [82]


SpirochinandAnthonine Anti-bacterial [90, 91]
Roots Campesterol, stigmasterol, β-sitosterol Diuretic [100]
andavenasterol
Steroids Sexual weakness [58]

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CONFLICT OF INTEREST nopharmacol 2008; 116(3): 439-46.
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[18] Aslam M, Anwar F, Nadeem R, Rashid U, Kazi TG, Nadeem M.
Declared none. Mineral composition of Moringa oleifera leaves and pods from dif-
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