ANALYSIS OF PROTEIN

CRYSTALLIZATION

1) PROTEIN
WHAT PURIFICATION
IS PROTEIN SAMPLE
Obtaining pure protein samples is
CRYSTALLOGRAPHY? PREPARATION
crucial for successful crystallography

Protein crystallography is a
2) CRYSTALLIZATION
cornerstone technique in
structural biology that allows Crystallization was done using
scientists to determine the three- crystallization robot. Crystallization
dimensional structure of proteins. robots can handle a large number of
samples and increase the chances of
obtaining high-quality crystals and
achieving optimal results

SAMPLE
DIFFRACTION
Protein crystals are exposed to intense X-
ray beams, and the resulting diffraction
patterns are recorded using specialized
detectors. Multiple data sets are
collected by rotating the crystal at
different orientations to capture the full
diffraction information.

MODEL
DATA ANALYSIS
BUILDING
The collected diffraction data
The electron density map is used to
undergoes a complex computational
build an initial model of the protein
process known as phase
structure. This model is refined
determination, where the phases of
iteratively using computational
the diffracted waves are calculated.
algorithms, considering factors such
The electron density map is then built,
as stereochemistry and experimental
refined, and interpreted to determine
restraints.
the protein's atomic structure.

Fahim Amali Bin Shamsul Asuadi (55223221004) CBB30204