RQ:

How does increasing concentration of sucrose solution (0.2 g/mL, 0.4 g/mL, 0.6
g/mL, 0.8 g/mL, 1 g/mL) affects the rate of osmosis, measured by the change in
mass (g) in carrot slices?

2. Hypothesis

Null hypothesis: If concentration of sucrose increases, then the rate of osmosis

increases too.

Alternative: If concentration of sucrose increases, then the rate of osmosis

decreases.

3. Variables:

Independent variable Dependent variable Controlled variables

Concentration of sucrose Rate of osmosis • Mass of each
carrot slice
• Species of carrot
• Sucrose
concentrations
Concentration of the First, weigh each carrot • To maintain this
sucrose solution will be slice before placing variable we have
0.2 g/mL, 0.4 g/mL, 0.6 them in the solutions. to cut them in a
g/mL, 0.8 g/mL, 1.0 After soaking them for way so every slice
g/mL. 40 minutes, take them will be the same
out, pat gently with a mass using the
By using different paper towel to remove equipment. In this
concentrations the extra solution, and case every carrots
osmotic gradient will be weigh them again. mass should be
shown that are Calculate the mass 0.80g.
experienced by carrot change by subtracting • Control this part
slices. Each of the the initial weight from by using the same
concentrations will be the final weight. Repeat type of carrot,
measured by measuring this process five times because different
 cylinder within 25 ml of for each concentration types shows
sucrose solution. level of the external different results.
Typically, the solubility solution. • To maintain the
ratio is considered as 1 consistency the
part solute to 25 parts different sucrose
solvent. This solution solutions have to
will affect the carrot be the same in
then. mass. In this case
25ml each one.

4. Method:
Materials and Equipment:

Materials Quantity
Carrot 1
Concentration of sucrose 0.2 ml , 0.4ml, 0.6ml, 0.8ml, 1ml
Measuring cylinder 1
Electronic scales 1
Timer 1
Tray 1
Knife 1
Paper towels 20
Graduated cylinder 1

Step-by-step method type:

• Get the carrot and cut it into slices.
• Cut the slices till 25 pieces.
• Weight all the slices initial mass using electronic scales.
• Organize pieces as 5 for each concentration.
• Bring the concentrations and organize by the %.
• Label the petri dishes with the solutions that are being used.
• Place a few carrot slices in each solution and Set a timer for 40 minutes.
• After 40 minutes weigh all the pieces and divide them depending on solution
percentages.
• Weigh the mass of each slice and record the data.
• Try for 5 times to each solution.
5. Data

Raw data:
Name: Quantative data. Collection of variables

Initial mass of Concentration Final mass of Time/Duration

carrot of sucrose carrot Min/minutes
(5 slices per solution (5 slices per
trial) mL/milliliters trial)
g/gramms g/gramms
Trial 1 0.80 0.2 1 slice – 1.05 40
2 slice – 1.33
3 slice – 0.92
4 slice – 1.08
5 slice – 1.12
Trial 2 0.80 0.4 1 slice – 1.12 40
2 slice – 0.75
3 slice – 0.94
4 slice – 1.04
5 slice – 0.91
Trial 3 0.80 0.6 1 slice – 0.76 40
2 slice – 0.73
3 slice – 1.17
4 slice – 1.05
5 slice – 1.01
Trial 4 0.80 0.8 1 slice – 0.64 40
2 slice – 1.06
3 slice – 0.79
4 slice – 0.6
5 slice – 0.62
Trial 5 0.80 1.0 1 slice – 0.54 40
2 slice – 0.46
3 slice – 0.93
4 slice – 1.09
5 slice – 0.55
Processed data:

Concentratio Change in mass of slice of carrot (g) Average Average

n of external change full
trial 1 trial 2 trial 3 trial 4 trial 5
solution in mass final
(g/mL) (g) mass
0 +0.25 +0.53 +0.12 +0.28 +0.32 0.3 1.1
0.2 +0.22 -0.05 +0.14 +0.34 +0.11 0.152 0.92
0.4 -0.04 -0.07 +0.37 +0.25 +0.21 0.144 0.94
0.6 -0.14 +0.26 -0.01 -0.2 -0.18 -0.054 0.74
0.8 -0.26 -0.34 +0.13 -0.29 -0.25 -0.336 1.19

Part 2

Null hypothesis: If concentration of sucrose increases, then the rate of osmosis

increases too.

Alternative: If concentration of sucrose increases, then the rate of osmosis

decreases.

Raw data:

Mass of carrot Concentration of Time for each Trial numbers

slices (grams/ g) sucrose trial for each
(milliliter/ ml) (minutes/min) concentration
per carrot
0.80 0.2 40 5
0.80 0.4 40 5
0.80 0.6 40 5
0.80 0.8 40 5
0.80 1.0 40 5
Processed data