Enzyme Concentration

Variables:

Independent variable - Enzyme concentration (potato size)

Dependent variable - The height of foam (bubbles)

Control variable - Temperature, amount of hydrogen peroxide

Hypothesis:

Null Hypothesis - The lesser the concentration of the enzyme, the level of foam created
increases after adding the catalyse.

Hypothesis - With more enzyme concentration (potato) the level of foam created will increase
and it can be shown through the bubbles that appear on the surface of the solution.

Experiment instruments needed:

- 3-4 cups/ (plastic cups are fine) plastic cups
- Potatoes
- Hydrogen peroxide (500 ml)
- Knife
- Weighing scale
- Stopwatch (phone timer)
- Gloves

Precautions:
- Make sure that you are wearing gloves (contact with peroxide can cause burns and
damage eye tissues)
- Make sure not to spill hydrogen peroxide on any cutlery (through away any in contact)
- Use goggles/glasses (if you have any )
- For girls tie your hair

Method:
1. Cut the potato in the preferred grams/ pieces.
2. Put 100 ml of hydrogen peroxide in a glass bowl.
3. Have a stopwatch ready and drop the potato piece into the hydrogen peroxide.
4. Immediately measure the height of the solution and start the timer for 2 minutes.
5. After 2 minutes, measure the new height and record the change.
 6. Repeat this experiment for 25g, 50g, 75g, 100g, 125g potato piece
7. Repeat the experiment, for each potato size, 4 times

Evaluation- note the temperature down

Cc
50 gram potato
Trial number Initial level (cm) After 2 min (cm) Change (cm)

1 4.5 5.5 1

2 4.8 5 0.2

3 4.5 5 0.5

4 4.5 5 0.5

Total average 18.3/4 20.5/4 2.2/4

=4.5 =5.1 =0.55

Shaista (weight of potato was around 75g)

Trial number Initial level (cm) After 2 min (cm) Change

1 6.6 7.4 0.8

2 7.1 7.6 0.5

3 6.9 7.5 0.6

Average 6.86 7.5 0.63

Maanasa - 25g potato

Trial number Initial level (cm) After 2 min (cm) Change

1 5.4 5.8 0.4

2 5.5 6.1 0.6

3 5.5 5.9 0.4

4 5.3 5.9 0.6

Average 5.425 5.925 0.5

Riya Chouhan - 125 gm potato (100ml hydrogen peroxide each)

Trial number Initial level (cm) After 2 min (cm) Change

1 5.1cm 7.3 cm 2.2cm

2 5cm 7.1cm 2.1cm

3 5cm 6.9 cm 1.9cm

4 5.2cm 7.5 cm 2.4cm

Total average 5.025 7.2 2.15

Rhea Kassam - 100g potato

Trial Number Initial level (cm) After 2 min (cm) Change

1 5 cm 6 cm 1 cm

2 4.5 cm 5 cm 0.5 cm

3 4 cm 5 cm 1 cm

4 4 cm 5 cm 1 cm

Total average 4.375 cm 5.25 cm 0. 875