Professional Documents
Culture Documents
Part 1
The major physiologic function of the immune system:
Extracellular bacteria
Intracellular bacteria
Fungi
Viruses
o Intracellular bacteria
o Virus
Extracellular bacteria – innate immunity
1. Complement activation via peptidoglycans (G+) and LPS (G-) ->>
Opsonisation and phagocytosis
Membrane attack complex
Extracellular bacteria – innate immunity
1. Complement activation via peptidoglycans (G+) and LPS (G-) ->>
TH1
o Intracellular bacteria
o Virus
Intracellular bacteria – innate immunity
1. Activation of complement?
The formation of granulomas surrounding infected cells may cause necrosis and
fibrosis of the tissues responsible of clinical symptoms (bacteria is in a latent stage)
o Intracellular bacteria
o Virus
Virus – innate immunity
1. Response of infected cells:
Secretion of Type I interferon ->> Recognition of viral RNA and DNA via TLRs
Latent infections – Viral DNA persists in the host cell but does not replicate
Sometimes, viruses do not cause the disease but the immune system - Hepatitis B
Mechanisms of immune evasion by virus
Guirado E, Schlesinger L.
2013; Frontiers in Immunology
DOI:10.3389/fimmu.2013.00098
Mycobacterium tuberculosis (Mtb)
Live attenuated bacteria
Bacillus Calmette-Guerin (BCG) – Modified Mycobacterium bovis sp. without infective antigens
Protective only against TB meningitis and disseminated TB in children under 5, but not
adult pulmonary TB and it does not stop transmission
BCG does not induce humoral immunity and only partial T-cell response
HIV+ patients, reservoirs for Mtb – Potential bacteria spreaders
Ivanyi I. 2021; Tuberculosis
doi.org/10.1016/j.tube.2020.102021
Mycobacterium tuberculosis (Mtb)
New approaches:
Subunits constructs ->> Fusion of proteins containing both replicating and latent stage
antigens, adjuvants, recombinant formulations and genetically modified
BCG or attenuated Mtb
(Voss G, 2018; Andersen P, 2019; Kauffman SHE, 2020)
Viral vaccines ->> Chimp adenovirus expressing Mtb antigens
(McShane H, 2015; Xing Z, 2019)
Live attenuated bacteria ->> Mtb with gene deletions for certain antigens
(Tameris M, 2019; Spertini F, 2015; Aguilo N, 2016; Arbues A, 2013)
Viremia
www.polioeradication.org
1st February 2023
Strategies for active vaccine development
Type of vaccine Examples
Live attenuated or killed bacteria Bacillus Calmette-Guerin
Cholera
Live attenuated virus Polio - OPV
Yellow fever
Rabies
Inactivated vaccines Polio - IPV
Influenza virus
Subunit (antigen) vaccines Tetanus toxoid
Diphtheria toxoid
Conjugate vaccines Pneumococcus
Haemophillus influenzae
Synthetic vaccines Hepatitis (recombinant proteins)