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What is Protozoa?
Protozoa are unicellular, eukaryotic, heterotrophic organisms.
They are either free-living or parasites.
There are around 65000 species of protozoans categorised in different groups.
They lack a cell wall.
There are many different cell organelles, that perform various tasks performed by
different organs in higher animals, e.g. mouth, anus, intestinal tract, etc.
There are many protozoa, that cause various diseases in animals and humans, e.g.
Plasmodium (malarial parasite),
Trypanosoma (sleeping sickness),
Trichomonas (trichomoniasis), etc.
Plasmodium (malarial parasite),
Trichomonas (trichomoniasis)
Size and Shape- The size and shape of Protozoa vary greatly, from microbial (1µm) to
large enough and can be seen by the naked eye. The shell of unicellular foraminifera
can have a diameter of 20 cm.
They lack a rigid cell wall, so they are flexible and found in various shapes. Cells are
enclosed in a thin plasma membrane. Some of the species have a hard shell on the outer
surface. In some of the protozoans especially in ciliates, the cell is supported
by Pellicle, which may be flexible or rigid and give organisms the definite shape and
help in locomotion.
Cellular Structure-
They are unicellular having a eukaryotic cell.
The metabolic functions are performed by some specialized internal structures.
They mostly have one membrane-bound nucleus in the cell.
The nucleus has diffused appearance due to scattered chromatin, the vesicular nucleus
contains a central body called endosome or nucleoli.
Nucleoli of apicomplexans have DNA, whereas amoeboids lack DNA in their
endosome.
Ciliates have micronucleus and macronucleus.
The plasma membrane encloses the cytoplasm and other locomotory projections like
flagella, pseudopodia and cilia.
Some of the genera have a membranous envelope called pellicle, which gives a
definite shape to the cell.
In some of the protozoans, epibiotic bacteria attach to the pellicle by their fimbriae.
The cytoplasm is differentiated into outer ectoplasm and inner endoplasm,
ectoplasm is transparent and endoplasm contains cell organelles.
Some of the protozoa have cytostome for ingesting food.
Food vacuoles are present, where ingested food comes.
Ciliates have a gullet, a body cavity which opens outside.
The central vacuole is present for osmoregulation, that removes excess water.
Membrane-bound cell organelles, like mitochondria, Golgi bodies, lysosomes and
other specialised structures are present.
Nutrition;
Protozoa are heterotrophic and have holozoic nutrition. They ingest their food by
phagocytosis.
Some of the protozoan groups have a specialised structure called cytostome for
phagocytosis.
The pseudopodia of amoeboids help in catching the prey.
Thousands of cilia present in ciliates drive the food-laden water into the gullet.
The ingested food comes to the food vacuole and gets acted on by lysosomal
enzymes. The digested food gets distributed throughout the cell.
Locomotion
Most of the protozoa species have flagella, cilia or pseudopodia.
Sporozoa, which don’t have any locomotory structure, have subpellicular
microtubules, which help in the slow movement.
Life Cycle
The life cycle of most of the protozoa alternates between dormant cyst stage and
proliferating vegetative stage, e.g. trophozoites.
The cyst stage can survive harsh conditions without water and nutrients.
It can remain outside the host for a longer duration and get transmitted.
The trophozoite stage is infectious, and they feed and multiply during this stage.
Reproduction
Mostly they reproduce by asexual means.
They multiply by binary fission, longitudinal fission, transverse fission or budding.
In some of the species, sexual reproduction is present.
The sexual reproduction is by conjugation, syngamy or by gametocytes formation.
Protozoa Classification and Examples
Protozoa is a phylum having unicellular heterotrophs.
It comes under Kingdom Protista.
Protozoa are divided into four major groups based on the structure and the part
involved in the locomotion:
Examples
A few diseases caused by fungi are –
Ringworm
Jock itch
Athlete’s foot
Aspergillosis
Candidiasis
Coelenterata
Phylum Coelenterata is a group of aquatic, or marine
organisms and a member of the Animal kingdom. They are
usually found attached to the rocks at the bottom of the
sea. These are the multicellular and simplest group of
invertebrate animals, found in colonies or solitarily.
Characteristics of Coelenterata
These are mostly aquatic or marine habitat animals.
These species exhibit a tissue-level organization.
The mouth is enclosed by thin and short tentacles.
They are diploblastic animals, in which, the body is
made up of two layers of cells:
Ectoderm – One layer makes up the cells outside the
body
Endoderm – the other forms the inner lining of the body.
They have cavities in their body.
The body is radially symmetrical.
The digestion is both intracellular
and extracellular.
The nervous system and the
circulatory system is absent.
They excrete and respire through
simple diffusion.
The mode of reproduction is
asexual, which is through budding.
The sexual mode of reproduction is
seen only in a few Coelenterates.
Anthozoa
Scyphozoa
Turbellaria
Trematoda
Cestoda
Turbellaria
These are free-living organisms found mostly in fresh water.
The body is dorsoventrally flattened.
Hooks and suckers are not present.
For eg., Planaria, Otoplana
Trematoda
Cestodes (tapeworms)
and Digenes (flukes) cause diseases in
humans and their livestock,
whilst monogeneans can cause serious
losses of stocks in fish farms.
Schistosomiasis, also known as
bilharzia or snail fever, is the second-
most devastating parasitic disease in
tropical countries, behind malaria.
Nemathehelminthes (Nematoda)
The nematodes or roundworms constitute the phylum Nematoda (also called
Nemathelminthes), The word "nematode" comes from a Greek word nema that means
"thread". with plant-parasitic nematodes also known as eelworms. They are a diverse
animal phylum inhabiting a broad range of environments.
The organisms belonging to the phylum Nematoda are also known as “roundworms”.
There are 28000 species of Nematoda identified till date.
They are unsegmented vermiform animals.
The epidermis has dorsal and ventral nerve cords.
The Nematodes present in the soil feed on the bacteria, fungi, and other nematodes,
and play an important role in nutrient recycling.
They also attack the insects and control the pests.
However, they cause severe damage to plants.
They feed on the plant roots and reduce the nutrient uptake and stress tolerance of the
plant.
A spadeful of soil contains numerous Nematodes.
They cause diseases such as Ascariasis, Trichuriasis, Hookworm, Enterobiasis,
Filariasis, and Angiostrongyliasis in humans.
Characteristics of Nematoda
Following are the important characteristics of Nematoda:
Their body is bilaterally symmetrical and triploblastic.
They are cylindrical in shape.
They exhibit tissue level organization.
Their body has a cavity or pseudocoelom.
The alimentary canal is distinct, with the mouth and the anus.
They are sexually dimorphic.
They are devoid of the circulatory system and respiratory system.
They are free-living or parasitic.
Parasitic nematodes cause diseases in the host.
Fertilization is internal and reproduction is sexual.
Their cuticle moults periodically.
The epidermis is synctical and contains dorsal or ventral nerve cords.
The body-wall muscles are longitudinal.
They possess amoeboid sperm cells.
They consist of chemosensory organs called aphids situated on the lips.
Classification of Nematodes
Phasmidia or Secernentea
These are mostly parasitic.
Caudal glands are absent.
Strongylida
They are vertebrate parasites devoid of lips.
The pharynx has no bulb.
They have a well-developed buccal capsule.
They possess a true copulatory bursa.
For eg., Strongylus
Oxyurida
They can be small or moderate in size.
Males have copulatory spicules.
Caudal alae are present.
They can be invertebrates or vertebrates.
The mouth consists of 3-4 simple lips.
For eg., Oxyuris
Ascaridida
These are oviparous, large stout nematodes
living as parasites in the intestine of the
vertebrates.
The pharynx may or may not contain a
posterior bulb.
Mouth possess 3 prominent lips.
There is no buccal capsule.
For eg., Ascaris
Spirurida
These are thread-like organisms that vary in
size from moderate to large.
The pharynx is devoid of bulb.
The females are larger than males and can be
oviparous or viviparous.
The mouth contains two prominent lips.
For eg., Spirura
Trichuroida
These are commonly known as whip-
worms.
They possess a slender pharynx.
The mouth is devoid of lips.
For eg., Trichuris
Camallanida
These are oviparous, thread-like
organisms.
The males have no bursa.
The bursa of adult females is
degenerated.
For eg., Camallanus
Aphasmidia or Adenophorea
They are free-living organisms.
The excretory system has no lateral canals.
Caudal glands are present.
Phasmids are absent.
Eg., Capillaria, Trichinella
Enoplida
They are mostly marine.
The cuticle contains bristles.
These are Cyanthiform amphids.
For eg., Anticoma
Dorylaimida
The cuticle is smooth without any bristles.
These are mostly terrestrial.
The buccal cavity consists of a protrusible
spear.
It consists of 6-10 labial papillae.
For eg., Trichodoris
Mermithida
The cuticle is smooth.
Amphids are reduced.
In the larval stage, they live as parasites,
whereas, the adult stage is free-living.
For eg., Mermis, Agamermis
Chromedorida
The cuticle is smooth or ringed.
The cuticle is devoid of any bristles.
There is a posterior bulb at the
pharynx.
They are free-living or marine.
For eg., Paracanthonchus
Desmoscolecida
The cuticle is ringed with prominent
bristles.
There are four sensory bristles at the
anterior end.
They are marine or free-living.
For eg., Desmoscolex
Araeolaimida
The cuticle is smooth and might or
might not contain bristles.
The amphids are spiral.
They possess labial papillae.
For eg., Plectus
Monohysterida
The cuticle is smooth, ringed and
contains bristles.
They possess circular amphids.
They can be marine, freshwater, or
terrestrial.
For eg., Monohystera
Worms in Humans
Plasmodium Life Cycle
Plasmodium is a genus of parasitic protozoans that fall under the family
Apicomplexa, the class Aconoidasida and the sporozoan subclass Coccidia.
Plasmodium is known to infect the red blood cells in mammals like humans, birds,
reptiles and so on.
P. knowlesi is the species of plasmodium that causes the infectious disease known as
Malaria.
Malaria occurs when the infected female Anopheles mosquito bites human beings or
any other mammal.
Some other species of plasmodium known to spread Malaria are P. vivax, P. ovale, P.
malariae, and P. knowlesi.
The plasmodium parasite displays a complex life cycle as it uses an insect (mosquito)
as a catalyst to carry and transmit the disease.
Life Cycle of Plasmodium:
Plasmodium parasites have a complex life cycle that includes three stages namely
Gametocytes, Sporozoites and Merozoites.
1) Gametocytes – Stage 1
The male gametocytes called microgametocytes and female gametocytes called
macrogametocytes are transmitted through an anopheles mosquito during a blood
meal.
The gametocytes within the mosquito develop into a sporozoite.
The male and female gametocytes mate inside the gut of the mosquito, and after 15 to
18 days, they form a parasite called sporozoite.
2) Sporozoites – Stage 2
When the infected mosquito feeds on humans, the sporozoites are transmitted
through the saliva into the bloodstream.
After that, the sporozoites enter the liver cells and here they mature into schizonts.
Later, these sporozoites rupture and release merozoites.
3) Merozoites – Stage 3
Over the next one or two weeks, each schizont multiplies in order to form several
other forms known as merozoites.
The merozoites exit from the lever, entering the bloodstream again and here they
attack the red blood cells.
The merozoites grow and multiply more while destroying all blood cells in that
process.
Certain merozoites develop into gametocytes, which is later ingested into the
bloodstream by a mosquito and the whole cycle starts again.
When the red blood cells are destroyed by the merozoites, it releases a toxin that
causes bone-shaking chills and fever.
Extreme cold chills and fever are classic symptoms of malaria in human beings.
Conclusion:
The evolution of plasmodium evolved in parallel with the vertebrate evolution for the
past 120 million years.
Therefore, the plasmodium has gone through new hosts, which is followed by
adaptation to those hosts, which shows the primary reason for the evolution of the
plasmodium.
Several species of plasmodium have been isolated from the chimpanzees that include,
P. gaboni, P. falciparum, P. ovale and P. reichenowi.
The other species are isolated from gorillas.
Plasmodium parasites found in reptiles are P. mexicanum and P. floridense and
plasmodium in birds include P. relictum and P. juxtanucleare.
Male mosquitoes have plumose
(bushy) antennae and the palpi are as
long as the proboscis and clubbed at the
end.
1) Cyst – Stage 1
Infection occurs by ingestion of mature cysts through fecally contaminated water or
food.
Due to protection from walls, cysts survive several days and sometimes weeks.
They are responsible for transmission.
2) Excystation – Stage 2
Excystation is the process by which cysts transform into trophozoites.
When the cysts enter the ileum of the small intestine of the host, the process of
excystation begins.
Trophozoites are released in the small intestine and from here they migrate to the
large intestine.
3) Trophozoite – Stage 3
Trophozoites are unicellular parasites that measure from 14 to 18 mm in diameter.
They multiply in the small intestine by binary fission to produce cysts that exit via
human stool.
Several trophozoites remain inside the lumen of the small intestine.
The rest attach themselves to the intestinal mucosa, enter the bloodstream and further
grow in the extraintestinal regions of the host like lungs, liver, brain.
Conclusion:
Entamoeba histolytica invasion in human hosts vary from showing zero symptoms to
being fatal.
This parasite, discovered in 1859 by Lambl. S. Chaudin, showed the difference
between pathogenic and non-pathogenic forms of amoeba.
Liver Fluke Life Cycle
Liver flukes, also known as Fasciola hepatica, are parasites that cause the liver fluke
disease known as Fascioliasis in the liver of human beings.
The liver fluke parasite falls under the phylum Platyhelminthes.
The liver fluke disease is caused when immature liver fluke parasites migrate through
the liver and are sometimes caused by the presence in bile ducts, or sometimes both.
Liver fluke infects all grazing animals like sheep, cattle and mammals like human
beings.
Liver fluke parasites occur in regions around water bodies like river banks, slow
streaming rivers, irrigation channels, as all these areas are suitable for hatching eggs of
the liver fluke.
The infestation of these parasites in animals like sheep, cattle have greatly impacted
the agricultural industry.
Life Cycle of Liver Fluke
Conclusion
Liver flukes are primarily large, flat parasitic worms that are found in the liver,
causing a disease known as fascioliasis.
Liver fluke parasites are most prominent in parts of Australia as well as in the regions
with several water bodies like irrigation channels, slow-streaming rivers and so on.
Economic Importance of Sponges
Why are sponges important?
Sponge grounds add structural complexity to those areas in the deep-sea where they
occur, providing habitat and refugia to commercially important species, supporting food
webs, and maintaining deep-sea biodiversity.
They play key roles in important biogeochemical cycles (silicon, carbon, and
nitrogen) and in bentho-pelagic coupling (i.e., in connecting ecological processes
occurring within the water column, or pelagic ‘realm’, with those taking place at (and
within) the seabed, the benthic ‘realm’).
Sponges are believed to constitute an impressive sink for silicon and grazed carbon,
and to act as a net source of particulate matter and nitrogen compounds, which may fuel
deep-sea microbial and invertebrate communities.
Sponges and their associated microorganisms have also been identified as having
significant biotechnological potential.
They are the single richest and most prolific source of marine natural products with
human health (i.e. pharmaceutical) applications.
Thousands of ‘sponge ground’ compounds have been discovered to date, with many
showing potent bioactivity and some, like barettin and ianthelline, possessing useful
anti-fouling and anti-cancer properties.
One of the most interesting potential applications is the use of the intricate silica-
based skeletons of sponges in novel approaches to tissue engineering and regenerative
medicine.