RUSSELL HIGH SCHOOL

LIFE SCIENCES: GRADE 10 MEMO

EXAMINER: M. AMOD PRACTICAL TEST
MODERATOR: F. HAFFEJEE TOTAL: 35 MARKS
DATE: 20-02-2023 TIME: 50 MINS
DNA: CHEMISTRY OF LIFE

Question 1

1.1 A - Proteins √
B - lipids / fats √
C - carbohydrates / glucose √
D - starch / carbohydrates √ (4)

1.2 A - Biurets Test / Millions Test √

C - Benedicts Solution / Fehlings A and B √
D - Iodine √ (3)

1.3 monosaccharides / glucose / saccharides √ (1)

1.4
• Superior source of energy
• Acts as an insulating layer under the skin
• Forms the structural component of cell membranes
• Acts as packing tissue between organs any 1 √√ (2)

1.5
• Do not add egg white to the test tube
• Do not add a protein to the test tube
• Use only water in the test tube any 1 √√ (2)

1.6
 Use gloves
 Use test tube tongs to hold the hot test tubes
 Do not drink the reagents
 Ensure that iodine is not spilled as it stains
 Place the mouth of the test tube facing away from the face
 Be careful when heating the glucose solution to prevent burns
any 3 √ each (3)
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Question 2

2.1.1 Temperature √

2.1.2 Units of sugar √

2.2
 He will use Fehlings A and Fehlings B √ /Benedict’s solution
 which should change from blue to green √ /yellow/orange

2.3
 Use many test tubes at each temperature √
 Repeat the investigation √ Any 2 √ each (2)

2.4
 Use the same amount of starch √
 Use the same amount of amylase √
 Use the same amount of water when making the solution of amylase and
the starch suspension √
 Use the same size and shape of the water bath √
 Use the same amount of water in the water bath √ Any 2 √ each (2)

2.5

Effect of temperature on the number of units of sugar produced

100

90

80

70

60
Units of Sugar

50

40

30

20

10

0
0 10 20 30 40 50 60 70

Temperature (0C)

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Mark Scheme:
Title (both variables): √
Label, unit and scale for x-axis: √
Label, unit and scale for y-axis: √
Correct shape of graph: √
All points correctly plotted: √√ (6)

2.6
 The enzyme becomes denatured √
 so the action of the enzyme decreases √ (2)

2.7 300 C √ and 400C √ (2)

2.8
 The enzyme is from the human body √
 and should function best at human body temperature √ / 370C (2)
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