Name: Gian Paul Lazo Date: 11-17-22

Section: BSN 1-D Subject: Anaphy Lab

Introduction

Cheek cells are eukaryotic (including a nucleus and other organelles within a
membrane) cells that are easily lost from the mouth lining. As a result, obtaining
them for observation is simple. Using biological stains such as methylene blue, it's
possible to clearly observe and differentiate the different parts of a cell.

Objectives

* What is the purpose of the methylene blue in the cheek cells

* Observing the cheek cell under the microscope

Materials
* Toothpick
* Slide/coverslip
* Methylene Blue

Procedure
1. With a toothpick; gently scrape the inside of your cheek.
2. Smear the cheek material from the toothpick on the clean microscope slide.
3. Place a drop of methylene blue (a stain) on the smear.
4. Place a coverslip on the slide touching one edge of the coverslip on the slide and
slowly lowering it on the slide and examine it under the microscope. The small, oval
structures inside the cells are the nuclei. The angular lines around the nuclei are the
plasma membranes.
5. Draw a single cell in the space provided and label the plasma membrane, nucleus,
and cytoplasm.

Guide Questions
1. What is the significance of using Meth blue in your experiment?
* Methylene blue is extremely helpful in the lab as a biological stain for the cheek cell
being viewed under a microscope.
2. What are the parts of the cheek cell?
* The parts of the cheek cell are cell membrane, Cytoplasm, Nucleus, organelle.

Conclusion

Without using the methylene blue in the experiment it’s very difficult for us to see the
the cheek cell in the microscope. Methylene blue makes the cell easier for us to spot
when observing the cells under the microscope.