Essay Communication Skills

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Essay Communication SkillsEssay Communication Skills
 Observation Of Gene Expression Using Various Plasmids
Observation of gene expression using various plasmids (pUC18/lux), and their role in E.
coli transformation

Nawaz Rahman

Panther ID: 5029032

Signature:____________________________________

Lab Partners:

Manuel Vera

Giselle Janoura

Jeniffer Marranca

Section U17

Abstract

Small circular pieces of DNA molecules located inside the nucleoid in bacterial species
(prokaryotes) are known

as Plasmids.Plasmids do not dictate the survival of the host bacteria, but it can give rise
to certain genes being

activated, if required, providing certain benefits. Survival and reproduction in different

environments can be considered

as one of these benefits. In this experiment we add different ... Show more content on
Helpwriting.net ...
Coli

forming into its own small colonies. No growth is also a possible outcome.

The experiment contained a total of six different petri dishes, each containing different
treatments to the E. coli.

The first petri dish held ampicillin resistance gene, luria broth and E. Coli. This served as
a positive control since there

was basically nothing to stop the E. Coli from growing. The petri dish containing E.Coli,
Luria Broth and ampicillin
with no plasmids provided us with the opposite results. There was no growth. Since there
were no plasmids in the dish

all the E. Coli died hence showing no growth.

In general E. Coli growth was directly proportional to the presence of ampicillin. If it so

happens there was

ampicillin in a petri dish with either the lux or ampicillin resistance gene, the result would
depict a colonial growth.

This is because the ampicillin and plasmid attack one another hence some die and the
others live. On the other hand if

there no ampicillin presents the petri dish would depict a lawn growth, because there is
no ampicillin there to replicate.

Introduction

We began the experiment by adding plasmids, a vector, to the bacteria E. Coli to see
the E. Coli growth with the plasmids in its DNA. Our results will conclude for us the
correlation between the transformation of a plasmid and the growth of E. Coli. The
process of
 Hester Prynne Religion
There is a Puritan society in the Scarlett letter which incorporates their organized
religion into the political system. Nathaniel Hawthorne wrote The Scarlet Letter and
inside of the text he implies a criticism and dislike of organized religion. The text itself
critiques the style of organized religion by describing the people as a group of people
with sad coloured garments and grey steeple crowned hats (Hawthorne 142). Hawthorne
pictures the people of the town, as a simple and dark people. The heathen of the story
who divided the code of the town, Hester Prynne, is described as beautiful from regularity
of feature and richness of complexion (Hawthorne 159). He illustrates how he believes
the actions of Hester Prynne might... Show more content on Helpwriting.net ...
Hester Prynne is forced to adhere to the regulations in order to keep her child whom
she loves so much. She says that she will instruct pearl about sin and what to believe.
Hester is a prime example of someone who wishes to resist the oppression, but is forced
by social standards and even economical ones to abide by them. She is paid by the
people who believe strongly from organized religion, so if she resists the entirety of the
oppression from the organized group then she would be out of money, a town to
socialize and live in, and her child which is her happiness she is my torture
(Hawthorne 325). Furthermore, Hester is her torment as well as happiness because if
not for her becoming pregnant, she would be in a normal house with her family and
friends, on the other hand Pearl is her happiness because it is still her child and she
loves her. Then, Hester, being brought up in this society herself, appeals to the emotion
of the minister to try and persuade them to let her keep Pearl. She says that God gave her
into my keeping and that She should have complete care of her (Hawthorne 325).
Furthermore, the organized religious group in The Scarlett Letter is oppressive
because they form their own through and ways of life upon all people of the town, as
well as any visitors to the area. The people are stopped from resisting this by
formulating the idea that there is no way for someone to resist it and