observe

be inspired
reveal
ATOLIGOMER

BE INSPIRED
The Sea and its benefits for the skin…
When sea water becomes
therapeutic: The Sea, mysterious and fascinating, is the origin
René Quinton, a XIXth of life. From the beginning of time, Man has
century scientist, tried to tame her. The rolling of her waves
described the appea- charged with spray soothes the spirit and
rance of life as follows:
in the pre-Cambrian invites you to set voyage, while the violence
oceans, individual of her storms quickly remind you of her
cells organised force and vitality.
themselves to
form multi-celled
creatures and The iodised air of the open sea…
enclosed between
themselves an
interior fluid which Its enlivening properties, revitalising and
remained unchanged soothing, are in essence due to its richness
in its composition: in trace elements. In the final analysis,
sea water.
there is nothing surprising about that since
It was a dog, aptly named only the Sea contains all the elements of
Sodium, who was the first Mendeleïev’s periodic table.
guinea pig for his historic
experiments: Quinton tapped his
blood and perfused him with 10 litres of
sea water in 12 hours! The dog eliminated
9 litres, limped a bit... and carried on
normally.

In a few weeks, Quinton proved that the

marine isotonic plasma, thanks to its
richness in soluble vital elements, is
THE physiological liquid of living beings;
sea water is not toxic, it can replace
all the interior fluid and is a major
therapeutic agent.
OBSERVE
Our daily life, especially for the city dwellers amongst us, has little
opportunity to take the waters. The skin is faced daily with numerous
attacks: ageing, tobacco, stress, pollution… against which it must be
protected.

Sea water became an accepted The minerals and trace elements play a fundamental role in the correct
therapy encouraged by numerous enzymatic function of the skin. They reinforce its protection systems
doctors. and maintain cutaneous balance. But their level undergoes strong
variations, fades and sometimes is even exhausted.
In 1907, the first marine clinic was
inaugurated. It was a complete
success and a world media Mineral deficiency rapidly leads to cutaneous disorders, sensitive skin,
event. reactive skin, inflammation and irritation with the most severe cases
The headline in The Intransigent of cutaneous stress sometimes leading to Psoriasis.
read: "The work of Pasteur has
given us a concept of disease,
that of René Quinton gives us
a concept of health”.

To transport the minerals as close as possible to the cells and revitalise

fatigued and stressed skin, CODIF Recherche et Nature have developed
Atoligomer.
Atoligomer is obtained by an original process, concentrating all the minerals
and trace-elements present in sea water, while taking care to desodify the
extract to re-constitute the best of sea-water.

What is sea water ?

“It is a serum which attacks no microbe in particular, but which gives the organic cell the
power to overcome all of them". (The Intransigeant,1907)
THE BENEFITS OF SEA WATER FOR THE SKIN…

On average the ocean contains 35 grams of salt per kilogram of sea water. If you consider the total volume
of the ocean, that represents 320 tonnes per square meter on the visible surface. Sodium Chloride (NaCl)
is only one of a great number of salts in the solution. 71 of the 92 natural elements have been detected in sea
water. Some perhaps have yet to be discovered, if they are present in too low a quantity.

Elements making up Sea Water

(in blue on the chart) Atoligomer is a concentrate of atomised Sea Water,
partially desodified, which contains all the macro elements
Mendeleïev’s classification and trace elements present in sea water.

Concentration of principal elements (mg/l) Sea Water Atoligomer

Chlorides 19 497 304 000

Magnesium 1318 85 500
Sodium 11 049 76 200
Potassium 408 26 600
Calcium 422 1280
Iron < 0.001 41
Zinc < 0.001 11
Manganese < 0.001 4.6
Copper < 0.001 2.3
Iodine 107 < 50

Comparison between the relative composition of sea water and blood serum

Sea water
Blood serum
Quinton saw this just by comparing the composition
Relative composition

of the interior fluid of multi-celled animals with sea

water.
The composition of sea water, very close to that of
blood serum, explains the success of the first marine
clinics, the ancestors of our Thalassotherapy and
Thermal Spa centres.

Na K Ca Mg Cl SO4 HPO4 HCO3

Roles of macro elements and trace elements in the skin

Strengthening weak
Hydration Immune System Anti Free Radicals Collagen Synthesis
hair and nails

Calcium
Copper
Magnesium
Manganese
Potassium
Selenium
Silicium
Sodium
Zinc
In-vitro Test: effect of Atoligomer on the vitality of dermal fibroblasts

Human fibroblasts were cultured in different media for 42 hours

The vitality of the cells is evaluated by the capacity of 0,14

the mitochondria to reduce a substance called MTT

(units of optical density)

0,12
(tetrazolium salt) during cellular respiration. The reduction +450%

Cellular Vitality
0,10
of MTT is followed by optical density (a violet compound 0,08
when it is reduced). 0,06
+350%
0,04
Medium 1 : isotonic saline medium (0.9% NaCl)
0,02
Medium 2 : saline medium supplemented with Calcium (1mM)
Medium 1 Medium 2 Medium 3 3
0

Ca+Mg Atoligomer
and Magnesium (0.5mM)
Medium 3 : saline medium comprising 0.9% Atoligomer

Thanks to its richness in sea minerals, Atoligomer stimulates the vitality of dermal fibroblasts.

In-vitro Test : effect of Atoligomer on epidermal keratinocytes

Human epidermis cells were incubated in different media for 24 hours at the end of which the cellular
vitality was evaluated using the MTT method.

0,45
(units of optical density)

0,40
Medium 1 : isotonic saline medium (0.9% NaCl) 0,35
Cellular Vitality

Medium 2 : saline medium supplemented with Calcium (1mM) 0,30

+300%
0,25
and Magnesium (0.5mM)
0,20
Medium 3 : saline medium comprising 0.9% Atoligomer 0,15
+100%
0,10
0,05

Medium 1 Medium 2 Medium 3

0
Concentrated in macro elements and trace elements,
Atoligomer stimulates the vitality of epidermal Ca+Mg Atoligomer

keratinocytes.

In-vitro Test : effect of Atoligomer on cellular cohesion

Human epidermal cells were cultured in different media:

Medium without Minerals Saline Medium only containing NaCl Saline Medium containing Atoligomer

High level of cellular mortality Absence of intercellular cohesion Very good vialbility and cellular cohesion

Atoligomer,thanks to its richness in trace elements, stimulates the vitality of skin cells:
fibroblasts and keratinocytes, thus reinforcing the viability and vitality of the skin as well
as its cohesion
REVEAL
Clinical Test : effect of Atoligomer on the local treatment of Psoriasis

The effectiveness of treatment with Atoligomer and UVA light was compared to the traditional
PUVAtherapy (local treatment with 8-methyl psoralene: 8-MOP combined with UVA light).

Protocol:
30 volunteers suffering from
BATHING THERAPY PUVATHERAPY
Psoriasis vulgaris.
Atoligomer : 0.13%
8-MOP : 0.06%

10 min : 5 weeks 10 weeks

Each treatment was made up
of two distinct phases: a stage Atoligomer alone 3 exposures/week 1 exposure/week
8-MOP alone Increasing UVA doses UVA Dose Constant
Atoligomer + 8-MOP (0.4 à 3.9J/cm2) (3.9J/cm2)
of bathing treatment followed
by a stage of PUVAtherapy.

Results:

Duration of treatment required to obtain Overall Efficacy Evaluated by the Patient:

decolourisation of the lesions
Evaluation of Atoligomer efficacy on the number of
14 13
16 squamae, the size of the erythema, reduction in pruritus
and improvement in lesion pigmentation.
11
14
Number of weeks

12
100 87
10
80
Efficacy (%)

8
60 50
6 40
40
4 20
0
Atoligomer 8-MOP 8-MOP
2

alone alone + Atoligomer

0
8-MOP Atoligomer 8-MOP
+ Atoligomer
Atoligomer by itself has 50% efficacy and activates the
The Atoligomer + 8-MOP combination effectiveness of the psoralenes.
produced significant decolourisation of It increases the effectiveness of 8-MOP from 40% to
the lesions in a much shorter period of 89% and the time to obtain decolourisation of the lesions
time: 3 weeks less compared to treatment is clearly reduced.
with 8-MOP by itself.

Atoligomer concentrates the trace elements which give Sea Water its
revitalising and remineralising properties and offers a hydro cure for
your skin.
This concentrate of atomised Sea Water will find its place in numerous
applications:

- treatment of tired and stressed skin

- treatment of fragile scalp
- treatment of broken nails and hair
- hydration of the skin
- treatment of skin that is reactive and subject to Psoriasis

INCI : Sea salt (and) Silica

Marine origin Hydrosoluble active ingredient in-vitro test in-vivo test ECOCERT validation
 Atoligomer

REVITALISING – REMINIERALISING

Cosmetic activities
Improvement in Cellular Cohesion

Source of minerals and trace elements

INCI Sea salt (and) Silica

emulsion : 0.1 to 5%
% utilisation recommended: body wrapping : 5 to 30%
to regenerate sea water : Atoligomer 1,25% + NaCl 2,25%

Technical department : Codif Recherche & Nature

infotech@codif-recherche-et-nature.com
Commercial department: Codif Recherche & Nature
commercial@codif-recherche-et-nature.com
70, rue du Commandant l’Herminier - BP11709
35417 Saint-Malo cedex - FRANCE
Tel : +33-2-23-18-31-07 Fax : +33-2-23-18-31-01
RPDOC067GB

www.codif-recherche-et-nature.com