Fundamentals Initiation Elongation Telomeres

Of DNA Of DNA Of DNA

Replication Replication Hayflick
3’ AH * 5’ Leading 3’ .M * 5’
* * * * * * .* * * * * * * .B
Telomeres Limit
¥B * * * * * * * .M Strand *M AM * * * * .* ••
00
B-
5’ 3’5’ 3’ 5’ 3’
SHAMPOO
••
Bosom ☒B• GB BB
Centromere • *o•→→* • *o•→→* • *o•→←← •

3’ 5’ .EE#-------Ms 3’ 5’ rep rep rep

* * * * * .*
¥••¥
AH AH AH AH AH AH ,µ µ .µ
Mtg .MG
Origin of replication
.

.* * * * * * * * .* .M * * * * * * * .M
A-T rich area •
•
5’ 3’ 5’ 3’ Ligase Telomeres
¥*☒ • 2 H. Bonds fuse dna ends
Pre-replication Requires less energy together Telomeres Do not code for RNA
Primase DNA Polymerase Type 111 DNA Polymerase Type 1 Prevent gene loss
protein complex Multiple ORIC
Enzyme Needs 3’ OH 5’ 3’ Exonuclease (RNA Primers)
RNA Primers of RNA primer Reads 3’ 5’
Cell Replication (Cycle)
Enables DNA poly. Read 3’ 5’ Synthesizes 5’ 3’
G1 S G2 Mitosis 2 cells Type 111 Synthesize 5’ 3’ Proofreading
DNA Replication to make dna 3’ 5’ Exonuclease
(ATP ) S.S.B.P Replication Bubble
Reads 3’ 5’
3’
Lead
5’
Helicase MiB UM ,M BiH Synthesize 5’ 3’ .M•iM*** MiB .*

"OLD" 3’ 5’ • '
. .
.B MiB um ,B☒B

Bo Bo Bo Bo Bo
3’ 5’ ¥•⇐ * Old 3’ 3’ 5’
Bo Bo Bo Bo Bo Bo Bo Bo Bo Bo New
Bt Bt Bt Bt Bt 3’ 5’ Leading
5’ 3’ ¥•☒@ Bo Bo Bo Bo Bo New Replication Fork
uM.M_Mi@MhM.M * * * * * * * .*
Strand
.

AM * * * * * * * .*
Bo Bo Bo Bo Bo Old * * * * * * * .*
S.S.B.P 3’
.

Semiconservative 5’ 3’ 5’ 3’5’ 3’ 5’ Lag

*• •• HIV T Cells
Nucleases Prevents parental 3’ 5’3’ 5’ 3’ 5’ NRTI’s (Didanosine) Nucleosides
5’ strands from reannealing .B * * rH- B- * * * .B Lagging •

3’
Old 5’
oh 3’Ba Ba Ba Ba Ba po43 - .M * * * * * * * .B Strand A
Bo Bo Bo Bo Bo ¥••¥ Bo Bo Bo Bo Bo Protects from nucleases 5’ 3’
5’ 3’
Bo Bo Bo Bo Bo 3’ 5’ Telomerase
5’ 3’ ¥•☒☒µ
Bo Bo Bo Bo Bo Okazaki Fragments
Old po43 - 5’ Bo Bo Bo Bo Bo RNA Primers Cancer Cells Telomerase * '

3’oh
DNA Stretches Termination BAHARI
DNA Replication Direction Helicase 3’ 5’ RNA iµa•
Unwind Supercoils
Of DNA C C A A T C
•

A A U C C C
5’ 3’ " Replication 8 A

t > t to
G G T T A G G G T T A G G G
Drugs ✗ Bo Bo Bo Bo Bo Bo Bo Bo Bo Bo 5’ 3’ DNA
- Bo Bo Bo Bo Bo Bo Bo Bo Bo Bo Bo Bo
Lipase Nuclease + Bo B
.* * * * * * * *
⑧e
→@
BB
Replication Fork S.S.B.P '

• •
'

Drugs
BB
Bo Bo Bo Bo Bo
Bo
Bei
Bo
Bei
ago RNA DNA
@ Bo Bo Bo Bo B.
Reverse Transcription
Be
-qpB
•%q Cancer Replicate Opoisomerases
Bo Bo Bo Bo Bo B. B. B. B. B.

•• •e
Top 1 Irinotecan/Topotecan 1
No ATP Telomerase Stem Cells
•• Eukaryotic cells
•%• Top 11 Etoposide/tenoposide 11
"

••
* *
* * * * * → 1V Prokaryotic cells
Bacterial Replicate ATP Required
DNA Replication Bi-directional Infections
Top 11 Flouroquinolones

Cell Biology | DNA Replication ☒ '

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