Lab no 6

Multiple sequence Alignment using Clustal W

Name: Waqas Ali
Roll no :SU91-BASFSM-S23-002
Submitted to: Dr Rabia Nawaz
TA: Miss Mehak
Date : 19-February-2024
INTRODUCTION
What is Clustal W?
Clustal W is a multiple sequence alignment program that is used to align two or more protein
or DNA sequences. It is a popular tool in bioinformatics and is used for a variety of purposes,
including:
Uses of Clustal W
 Gene identifications.
 Phylogenetic analysis
 Protein structure prediction
Lab protocol
Step 1: Searched and opened Mega.
Step 2: The following page appeared

Step 3: File" menu and selecting "Open File Session."

Step 4 : opened the FASTA file .

Step 5: Clicked on Align option.

Step 6 : Align with Clustal W .

Step 7 : pressed ctrl +A.

Step 8 :Alignment “ press ok”

Step 9:Alignment done.

Step 10: Clicked data selected the Export alignment (Mega format).

Step 11:saved file by name waqas.mega.

Step 12 :Clicked ok.

Step 13: ok that confirmation.

Result: Alignment was done.

File saved .